{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,26]],"date-time":"2025-09-26T00:21:16Z","timestamp":1758846076973,"version":"3.44.0"},"reference-count":16,"publisher":"BMJ","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clin Pathol"],"published-print":{"date-parts":[[1989,3]]},"abstract":"<jats:p>To evaluate the role of the expression of hepatitis B core antigen (HBcAg) in liver cell damage the immunoperoxidase staining pattern of cryostat liver biopsy specimens from 16 chronic carriers of hepatitis B surface antigen (HBsAg) was investigated using three different kinds of anti-HBc antibodies. Polyclonal antibody prepared from recombinant HBcAg seemed to be more sensitive in detecting HBcAg than did monoclonal antibody from the same antigen. The topographical distribution of HBcAg detected by these two antibodies was similar, showing a close correlation to the histological activity of disease. Furthermore, the predominant localisation of cytoplasmic HBcAg usually reflected an active and severe ongoing hepatitis. On the other hand, monoclonal antibody prepared from purified Dane particles resulted in the prominent cytoplasmic staining for HBcAg regardless of histological severity of the hepatitis. The quantitative expression and topographical distribution of HBcAg depended on the type of anti-HBc antibodies used.<\/jats:p>","DOI":"10.1136\/jcp.42.3.284","type":"journal-article","created":{"date-parts":[[2007,6,9]],"date-time":"2007-06-09T00:50:46Z","timestamp":1181350246000},"page":"284-288","source":"Crossref","is-referenced-by-count":11,"title":["Distribution of HBcAg in hepatitis B detected by immunoperoxidase staining with three different preparations of anti-HBc antibodies."],"prefix":"10.1136","volume":"42","author":[{"given":"S","family":"Kakumu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M","family":"Arao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K","family":"Yoshioka","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y","family":"Tsutsumi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M","family":"Inoue","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[1989,3,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1159\/000149918","article-title":"DNA polymerase, and e-antigen in two different categories of hepatitis B antigen carriers","volume":"5","author":"Nordenfeld, E.; particles, Kjellen L.Dane","year":"1975","unstructured":"Nordenfeld E, Kjellen L. Dane particles, DNA polymerase, and e-antigen in two different categories of hepatitis B antigen carriers. Intervirology 1975;5:225-32.","journal-title":"Intervirology"},{"key":"ref_2","first-page":"1","article-title":"Immunohistologic demonstration of hepatitis B viral antigens in liver with reference to its significance in liver injury","volume":"40","author":"Huang, S.N.; Neurath, A.R.","year":"1979","unstructured":"Huang SN, Neurath AR. Immunohistologic demonstration of hepatitis B viral antigens in liver with reference to its significance in liver injury. 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Intrahepatic distribution ofhepatitis B surface and core antigens in chronic hepatitis B virus infection. Gastroenterology 1987;92:220-5.","journal-title":"Gastroenterology"},{"key":"ref_7","first-page":"2773","article-title":"Specificity of T lymphocyte cytotoxicity to autologous hepatocytes in chronic hepatitis B virus infection: Evidence that T cells are directed against HBV core antigen expressed on hepatocytes","volume":"129","author":"Mondelli, M.; Mieli-Vergani, G.; Alberti, A.","year":"1982","unstructured":"Mondelli M, Mieli-Vergani G, Alberti A, et al. Specificity of T lymphocyte cytotoxicity to autologous hepatocytes in chronic hepatitis B virus infection: Evidence that T cells are directed against HBV core antigen expressed on hepatocytes. 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Intrahepatic distribution of hepatitis B virus (HBV)-associated and HLA class I antigens in patients with chronic liver disease B. Acta Hepatol Jpn 1987;28:1 149-56.","journal-title":"Acta Hepatol Jpn"},{"key":"ref_10","first-page":"914","article-title":"Acute and chronic hepatitis revisited","volume":"ii:","author":"group, International","year":"1977","unstructured":"International group. Acute and chronic hepatitis revisited. Lancet 1977;ii:914-9.","journal-title":"Lancet"},{"key":"ref_11","first-page":"1606","article-title":"Hepatitis B virus core antigen: synthesis in Escherichia coli","volume-title":"and application in diagnosis. Proc Natl Acad Sci","volume":"79","author":"Stahl, S.; MacKay, P.; Magazin, M.; Bruce, S.A.; Murray, K.","year":"1982","unstructured":"Stahl S, MacKay P, Magazin M, Bruce SA, Murray K. Hepatitis B virus core antigen: synthesis in Escherichia coli and application in diagnosis. 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