{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T13:21:38Z","timestamp":1760188898274,"version":"3.44.0"},"reference-count":12,"publisher":"BMJ","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clin Pathol"],"published-print":{"date-parts":[[1989,4]]},"abstract":"<jats:p>One hundred and sixty six bone marrow aspirates from children with abdominal neuroblastoma were independently examined for the presence of neuroblastoma cells by conventional haematological staining techniques and by indirect immunofluorescence using one or more of five monoclonal antibodies. Results using the two methods were evaluated by comparison with the results of bone marrow, bone and lymph node biopsy specimens taken at the same time, and in the light of the child's clinical course. One antibody, UJ13A, was found to have 98.5% specificity and 85.7% sensitivity, compared with sensitivities for conventional staining of 50% for aspirate alone and of aspirate or trephine biopsy specimen of 71.4%. The remaining antibodies UJ127\/11, UJ223\/8, UJ181\/4 and UJ167\/11, with specificities in the range 94-99%, were found to have sensitivities in the range 47-61%, lower than the sensitivity of conventionally stained aspirate or trephine biopsy specimen. Routine immunofluorescence staining with monoclonal antibody UJ13A should increase the sensitivity of detection of metastatic neuroblastoma.<\/jats:p>","DOI":"10.1136\/jcp.42.4.422","type":"journal-article","created":{"date-parts":[[2007,6,9]],"date-time":"2007-06-09T00:50:46Z","timestamp":1181350246000},"page":"422-426","source":"Crossref","is-referenced-by-count":34,"title":["Monoclonal antibodies for detecting bone marrow invasion by neuroblastoma."],"prefix":"10.1136","volume":"42","author":[{"given":"D W","family":"Rogers","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J G","family":"Treleaven","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J T","family":"Kemshead","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J","family":"Pritchard","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[1989,4,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1215","DOI":"10.1136\/jcp.36.11.1215","article-title":"The detection of bone marrow invasion by neuroblastoma is improved by sampling at two sites with both aspirate and trephine biopsy","volume":"36","author":"TM, I.Franklin; J, Pritchard","year":"1983","unstructured":"I Franklin TM, Pritchard J. The detection of bone marrow invasion by neuroblastoma is improved by sampling at two sites with both aspirate and trephine biopsy. J Clin Pathol 1983;36: 1215-8.","journal-title":"J Clin Pathol"},{"key":"ref_2","first-page":"92","article-title":"Colour atlas of paediatric haematology","author":"Hann, I.M.; Rankin, A.; Lake, B.D.; Pritchard, J.","year":"1983","unstructured":"Hann IM, Rankin A, Lake BD, Pritchard J. Colour atlas of paediatric haematology. Oxford: Oxford University Press, 1983;92-4."},{"key":"ref_3","first-page":"12","article-title":"Use of panels of monoclonal antibodies in the differential diagnosis of neuroblastoma and lymphoblastic disorders","volume":"i:","author":"Kemshead, J.T.; Goldman, A.; Fritschy, J.; Malpas, J.S.; Pritchard, J.","year":"1983","unstructured":"Kemshead JT, Goldman A, Fritschy J, Malpas JS, Pritchard J. Use of panels of monoclonal antibodies in the differential diagnosis of neuroblastoma and lymphoblastic disorders. Lancet 1983;i:12-4.","journal-title":"Lancet"},{"key":"ref_4","first-page":"637","article-title":"cytological and immunological analyses are complementary for the detection of neuroblastoma cells in bone marrow","volume":"54","author":"true","year":"1986","unstructured":"Favrot MC, Frappaz D, Maritaz 0, et al. Histological, cytological and immunological analyses are complementary for the detection of neuroblastoma cells in bone marrow. Br J Cancer 1986;54:637-41.","journal-title":"Br J Cancer"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"742","DOI":"10.1200\/JCO.1984.2.7.742","article-title":"Advanced neuroblastoma: improved response rate using a multiagent regimen (OPEC) including sequential cisplatin and VM-26","volume":"2","author":"Shafford, E.A.; Rogers, D.W.; Pritchard, J.","year":"1984","unstructured":"Shafford EA, Rogers DW, Pritchard J. Advanced neuroblastoma: improved response rate using a multiagent regimen (OPEC) including sequential cisplatin and VM-26. J Clin Oncol 1984;2:742-7.","journal-title":"J Clin Oncol"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"374","DOI":"10.1002\/1097-0142(197102)27:2<374::AID-CNCR2820270221>3.0.CO;2-G","article-title":"A proposed staging for children with neuroblastoma","volume":"27","author":"Evans, A.E.; D'Angio, G.J.; Randolph, J.","year":"1971","unstructured":"Evans AE, D'Angio GJ, Randolph J. A proposed staging for children with neuroblastoma. Cancer 1971;27:374-8.","journal-title":"Cancer"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"591","DOI":"10.1002\/ijc.2910310510","article-title":"Biological characterization and clinical applications of a monoclonal antibody recognizing an antigen restricted to neuroectodermal tissues","volume":"31","author":"Allan, P.M.; Garson, J.A.; El, Harper","year":"1983","unstructured":"Allan PM, Garson JA, Harper El, et al. Biological characterization and clinical applications of a monoclonal antibody recognizing an antigen restricted to neuroectodermal tissues. Int J Cancer 1983;31:591-8.","journal-title":"Int J Cancer"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1002\/ijc.2910310209","article-title":"UJ 127: 1 1 detects a 220,000-240,000 kdal glycoprotein present on a sub-set of neuroectodermally derived cells","volume":"31","year":"1983","unstructured":"UJ 127:1 1 detects a 220,000-240,000 kdal glycoprotein present on a sub-set of neuroectodermally derived cells. Inl J Cancer 1983;31:187-95.","journal-title":"Inl J Cancer"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"575","DOI":"10.1016\/S0046-8177(84)80012-X","article-title":"Neuron-specific enolase in the diagnosis of neuroblastoma and other small, round cell tuimors in children","volume":"15","author":"Tsokos, M.; Chandra, Linnoila R.I.; RS, Triche; T.J.","year":"1984","unstructured":"Tsokos M, Linnoila RI. Chandra RS, Triche TJ. Neuron-specific enolase in the diagnosis of neuroblastoma and other small, round cell tuimors in children. Hum Pathol 1984;15:575-84.","journal-title":"Hum Pathol"},{"key":"ref_10","first-page":"10","article-title":"High-resolution in situ hybridization technique using biotinylated n-myc oncogene probe reveals periodic structure of HSRs in human neuroblastoma","volume":"45","author":"Garson, J.A.; Van den Berghe, J.A.; Kemshead, J.T.","year":"1987","unstructured":"Garson JA, Van den Berghe JA, Kemshead JT. High-resolution in situ hybridization technique using biotinylated n-myc oncogene probe reveals periodic structure of HSRs in human neuroblastoma. Cvtogenet Cell Genet 1987;45:10-5. II Joshi SS, Kessinger A, Mann SL, et al. Detection of malignant cells in histologically normal bone marrow using culture techniques.","journal-title":"Cvtogenet Cell Genet; Detection of malignant cells in histologically normal bone marrow using culture techniques"},{"key":"ref_11","first-page":"303","article-title":"Pritchard Bone Marrows Transplantation","volume":"1","author":"Rokers, Treleaven; Kemshead","year":"1987","unstructured":"Rokers, Treleaven, Kemshead, Pritchard Bone Marrows Transplantation 1987;1:303-10."},{"issue":"1","key":"ref_12","first-page":"351","article-title":"Immunohistochemic; Al performance testing of monoclonal antibodies to neuroblastoma cells on normal adrenals, spinal and sympathetic ganglia, and neural crest tumours","volume":"1","author":"Oppedal, B.R.; Brandtzaeg, P.; Kemshead, J.T.","year":"1987","unstructured":"Oppedal BR, Brandtzaeg P, Kemshead JT. Immunohistochemic;Al performance testing of monoclonal antibodies to neuroblastoma cells on normal adrenals, spinal and sympathetic ganglia, and neural crest tumours. Histopacthologv 1987;1 1: 351-62.","journal-title":"Histopacthologv"}],"container-title":["Journal of Clinical Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1136\/jcp.42.4.422","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,9,25]],"date-time":"2025-09-25T13:14:16Z","timestamp":1758806056000},"score":1,"resource":{"primary":{"URL":"https:\/\/jcp.bmj.com\/lookup\/doi\/10.1136\/jcp.42.4.422"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1989,4]]},"references-count":12,"journal-issue":{"issue":"4","published-online":{"date-parts":[[1989,4,1]]},"published-print":{"date-parts":[[1989,4]]}},"alternative-id":["10.1136\/jcp.42.4.422"],"URL":"https:\/\/doi.org\/10.1136\/jcp.42.4.422","relation":{},"ISSN":["0021-9746","1472-4146"],"issn-type":[{"type":"print","value":"0021-9746"},{"type":"electronic","value":"1472-4146"}],"subject":[],"published":{"date-parts":[[1989,4]]}}}