{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T21:09:14Z","timestamp":1792271354703,"version":"4.4.0"},"reference-count":9,"publisher":"BMJ","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clin Pathol"],"published-print":{"date-parts":[[1989,6]]},"abstract":"<jats:p>The API 20NE kit and a simple screening system involving Gram's stain, the oxidase reaction, colistin and gentamicin resistance, and colonial characteristics on a differential agar medium, were used to test 400 strains of Pseudomonas pseudomallei. The API kit identified 390 (97.5%) strains correctly on first testing and all but one of the remainder on second testing. Only one strain was initially misidentified (as Ps cepacia). The screening system was 100% accurate in identifying Ps pseudomallei. In non-endemic areas the API 20NE kit may be used to identify sporadic imported strains of Ps pseudomallei. Such kits may also help to delineate the geographical distribution of melioidosis. In endemic areas the screening tests described offer a cheap, simple, and accurate means of presumptively identifying Ps pseudomallei from clinical specimens.<\/jats:p>","DOI":"10.1136\/jcp.42.6.645","type":"journal-article","created":{"date-parts":[[2007,6,9]],"date-time":"2007-06-09T00:50:46Z","timestamp":1181350246000},"page":"645-648","source":"Crossref","is-referenced-by-count":116,"title":["Identification of Pseudomonas pseudomallei in clinical practice: use of simple screening tests and API 20NE."],"prefix":"10.1136","volume":"42","author":[{"given":"D A","family":"Dance","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V","family":"Wuthiekanun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P","family":"Naigowit","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N J","family":"White","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"239","published-online":{"date-parts":[[1989,6,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"965","DOI":"10.1016\/0002-9343(88)90080-0","article-title":"Melioidosis: a reminder","volume":"84","author":"Morrison, R.E.; Lamb, A.S.; Craig, D.B.; Johnson, W.M.","year":"1988","unstructured":"Morrison RE, Lamb AS, Craig DB, Johnson WM. Melioidosis: a reminder. Am J Med 1988;84:965-7.","journal-title":"Am J Med"},{"key":"ref_2","first-page":"655","article-title":"Septic melioidosis after a visit to south east Asia","volume":"5","author":"Bouvy, J.J.; Degener, J.E.; Stijnen, C.; Gallee, M.P.W.; van der Berg, B.","year":"1987","unstructured":"Bouvy JJ, Degener JE, Stijnen C, Gallee MPW, van der Berg B. Septic melioidosis after a visit to south east Asia. Eur J Clin Microbiol 1987;5:655-6.","journal-title":"Eur J Clin Microbiol"},{"key":"ref_3","first-page":"777","article-title":"Melioidosis in Bangladesh: a case report. Trans R Soc Trop MedHyg","volume":"82","author":"Struelens, M.; Mondol, G.; Bennish, M.; Dance, D.A.B.","year":"1988","unstructured":"Struelens M, Mondol G, Bennish M, Dance DAB. Melioidosis in Bangladesh: a case report. 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Pathology 1979;11: 293-7.","journal-title":"Pathology"},{"issue":"11","key":"ref_6","first-page":"393","article-title":"Melioidosis, with special reference to the dissociation of Bacillus whitmori","volume":"1930","author":"Nicholls, L.","unstructured":"Nicholls L. Melioidosis, with special reference to the dissociation of Bacillus whitmori. Br J Exp Pathol 1930;11:393-9.","journal-title":"Br J Exp Pathol"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"500","DOI":"10.1136\/jcp.32.5.500","article-title":"Identification of Pseudomonas pseudomallei in the clinical laboratory","volume":"32","author":"Ashdown, L.R.","year":"1979","unstructured":"Ashdown LR. Identification of Pseudomonas pseudomallei in the clinical laboratory. J Clin Pathol 1979;32:500-4.","journal-title":"J Clin Pathol"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/0378-1135(83)90009-3","article-title":"Evaluation of the API 20E and Microbact 24E systems in the identification of Ps pseudomallei","volume":"8","author":"Thomas, A.D.","year":"1983","unstructured":"Thomas AD. Evaluation of the API 20E and Microbact 24E systems in the identification of Ps pseudomallei. Veterinary Microbiology 1983;8:61 1-5.","journal-title":"Veterinary Microbiology"},{"key":"ref_9","first-page":"6","article-title":"Advisory Committee for Dangerous Pathogens. Categorisation of pathogens according to hazard and categories of containment","year":"1984","unstructured":"Advisory Committee for Dangerous Pathogens. Categorisation of pathogens according to hazard and categories of containment. London: HMSO, 1984:6."}],"container-title":["Journal of Clinical Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1136\/jcp.42.6.645","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,9,25]],"date-time":"2025-09-25T12:03:28Z","timestamp":1758801808000},"score":1,"resource":{"primary":{"URL":"https:\/\/jcp.bmj.com\/lookup\/doi\/10.1136\/jcp.42.6.645"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1989,6]]},"references-count":9,"journal-issue":{"issue":"6","published-online":{"date-parts":[[1989,6,1]]},"published-print":{"date-parts":[[1989,6]]}},"alternative-id":["10.1136\/jcp.42.6.645"],"URL":"https:\/\/doi.org\/10.1136\/jcp.42.6.645","relation":{},"ISSN":["0021-9746","1472-4146"],"issn-type":[{"value":"0021-9746","type":"print"},{"value":"1472-4146","type":"electronic"}],"subject":[],"published":{"date-parts":[[1989,6]]}}}