{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T17:37:24Z","timestamp":1760204244808,"version":"3.44.0"},"reference-count":8,"publisher":"BMJ","issue":"10","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clin Pathol"],"published-print":{"date-parts":[[1990,10]]},"abstract":"<jats:p>Following the outbreak of Legionnaires' disease in Stafford in 1985, 500 serum samples were submitted to the indirect immunofluorescence antibody test and a latex agglutination. Latex agglutination using ultrasonically disrupted Legionella pneumophila antigens coupled to latex particles, proved a rapid, simple method for detecting circulating antibodies to L pneumophila in a one minute slide latex agglutination test. There was good correlation with the indirect immunofluorescence antibody test (IFAT), and the specificity and sensitivity with respect to a diagnostic result were 98.3% and 97.6%, respectively, using a series of well characterised sera. The latex agglutination test seems well suited as a screening test for presumptive cases of Legionnaires' disease; the latex reagent is easy to prepare and seems to remain stable at 4 degrees C for up to six months.<\/jats:p>","DOI":"10.1136\/jcp.43.10.860","type":"journal-article","created":{"date-parts":[[2007,6,9]],"date-time":"2007-06-09T00:50:46Z","timestamp":1181350246000},"page":"860-862","source":"Crossref","is-referenced-by-count":6,"title":["Use of latex agglutination technique for detecting Legionella pneumophila (serogroup 1) antibodies."],"prefix":"10.1136","volume":"43","author":[{"given":"M G","family":"Holliday","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[1990,10,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1136\/jcp.35.2.211","article-title":"Diagnosis of Legionella pneumophila infections by means of formolised yolk sac antigens","volume":"35","author":"Harrison, T.G.; Taylor, A.G.","year":"1982","unstructured":"Harrison TG, Taylor AG. Diagnosis of Legionella pneumophila infections by means of formolised yolk sac antigens. J Clin Pathol 1982;35:211-4.","journal-title":"J Clin Pathol"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1028","DOI":"10.1136\/jcp.35.9.1028","article-title":"A rapid microagglutination test for the diagnosis of Legionella pneumophila (serogroup 1) infection","volume":"35","author":"Harrison, T.G.; Taylor, A.G.","year":"1982","unstructured":"Harrison TG, Taylor AG. A rapid microagglutination test for the diagnosis of Legionella pneumophila (serogroup 1) infection. 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Med Lab Sci 1987;44: 334-40.","journal-title":"Med Lab Sci"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"465","DOI":"10.1016\/S0140-6736(86)92177-X","article-title":"Latex agglutination test for Legionella antibody","volume":"ii:","author":"Holliday, M.G.","year":"1986","unstructured":"Holliday MG. Latex agglutination test for Legionella antibody. Lancet 1986;ii:465-6.","journal-title":"Lancet"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1174","DOI":"10.1136\/jcp.33.12.1174","article-title":"The diagnosis of Legionnaires' Disease by counterimmunoelectrophoresis","volume":"33","author":"Holliday, M.G.","year":"1980","unstructured":"Holliday MG. The diagnosis of Legionnaires' Disease by counterimmunoelectrophoresis. J Clin Pathol 1980;33: 1174-8.","journal-title":"J Clin Pathol"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1079","DOI":"10.1136\/jcp.25.12.1079","article-title":"Latex agglutination in the diagnosis ofmeningococcal meningitis","volume":"25","author":"Severin, W.P.J.","year":"1972","unstructured":"Severin WPJ. Latex agglutination in the diagnosis ofmeningococcal meningitis. J Clin Pathol 1972;25:1079-82.","journal-title":"J Clin Pathol"},{"key":"ref_8","article-title":"Beyond normality. The predictive value and efficiency of medical diagnosis","author":"Galen, R.S.; Gambino, SR","year":"1975","unstructured":"Galen RS, Gambino SR. Beyond normality. The predictive value and efficiency of medical diagnosis. New York: Wiley John & Sons: 1975."}],"container-title":["Journal of Clinical Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1136\/jcp.43.10.860","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,9,25]],"date-time":"2025-09-25T17:09:56Z","timestamp":1758820196000},"score":1,"resource":{"primary":{"URL":"https:\/\/jcp.bmj.com\/lookup\/doi\/10.1136\/jcp.43.10.860"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1990,10]]},"references-count":8,"journal-issue":{"issue":"10","published-online":{"date-parts":[[1990,10,1]]},"published-print":{"date-parts":[[1990,10]]}},"alternative-id":["10.1136\/jcp.43.10.860"],"URL":"https:\/\/doi.org\/10.1136\/jcp.43.10.860","relation":{},"ISSN":["0021-9746","1472-4146"],"issn-type":[{"type":"print","value":"0021-9746"},{"type":"electronic","value":"1472-4146"}],"subject":[],"published":{"date-parts":[[1990,10]]}}}