{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,13]],"date-time":"2025-12-13T04:38:20Z","timestamp":1765600700396,"version":"3.48.0"},"reference-count":10,"publisher":"BMJ","issue":"11","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clin Pathol"],"published-print":{"date-parts":[[1990,11]]},"abstract":"<jats:p>Serum samples from 20 Indian children with diarrhoea were compared with those from 20 children resident in the United Kingdom who had been diagnosed as having ulcerative colitis, or Crohn's disease, or indeterminate colitis using enzyme linked immunosorbent assays specific for Entamoeba histolytica and Giardia lamblia. More than 50% of the United Kingdom patients had high IgG responses in ELISAs for E histolytica and G lamblia. A confirmatory ELISA showed that the British sera reacted specifically to bovine serum proteins rather than to protozoal antigens. Prior incubation of sera with 5% bovine serum prohibited this reaction. Bovine serum is an integral part of the crude soluble antigen used in most ELISAs for E histolytica and G lamblia and needs to be replaced with purified antigen preparations. The British sera also reacted to other commonly used blocking agents such as bovine serum albumin, casein, and normal sheep serum. These reactions were attributed to uptake of dietary antigens or an enhanced immunological response to these antigens in patients with inflammatory bowel disease.<\/jats:p>","DOI":"10.1136\/jcp.43.11.950","type":"journal-article","created":{"date-parts":[[2007,6,9]],"date-time":"2007-06-09T00:50:46Z","timestamp":1181350246000},"page":"950-952","source":"Crossref","is-referenced-by-count":4,"title":["Non-specific reactions in enzyme linked immunosorbent assays for serum antibody to entamoeba histolytica and Giardia lamblia in non-endemic areas."],"prefix":"10.1136","volume":"43","author":[{"given":"N P","family":"Shetty","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I S","family":"Raj","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R S","family":"Macaden","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[1990,11,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1007\/BF02232727","article-title":"The indirect haemagglutination test for amoebiasis in patients with inflammatory bowel disease","volume":"17","author":"Healy, G.R.; Kraft, S.C.","year":"1972","unstructured":"Healy GR, Kraft SC. The indirect haemagglutination test for amoebiasis in patients with inflammatory bowel disease. Am J Dig Dis 1972;17:97-9.","journal-title":"Am J Dig Dis"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"630","DOI":"10.1016\/0035-9203(82)90227-9","article-title":"The specificity of serum antibodies to Giardia lamblia and to enterobacteria in gastrointestinal disease","volume":"76","author":"Moody, A.H.; Ridley, D.S.; Tomkins, A.M.; Wright, S.G.","year":"1982","unstructured":"Moody AH, Ridley DS, Tomkins AM, Wright SG. The specificity of serum antibodies to Giardia lamblia and to enterobacteria in gastrointestinal disease. 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The National Medical Journal of India 1989;2:1 19-21.","journal-title":"The National Medical Journal of India"},{"key":"ref_5","first-page":"184","article-title":"Diagnosis of giardiasis by specific IgM antibody ELISA","volume":"ii:","author":"Goka, A.K.J.; Rolston, D.D.K.; Mathan, VI, Farthing; M.J.G.","year":"1986","unstructured":"Goka AKJ, Rolston DDK, Mathan VI, Farthing MJG. Diagnosis of giardiasis by specific IgM antibody ELISA. Lancet 1986;ii:184-6.","journal-title":"Lancet"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1080\/01971528708063053","article-title":"The use of 3,3',5,5'-tetramethyl benzidine as a peroxidase substrate in microplate enzyme linked immunosorbent assay","volume":"8","author":"Goka, A.K.J.; Farthing, M.J.G.","year":"1987","unstructured":"Goka AKJ, Farthing MJG. The use of 3,3',5,5'-tetramethyl benzidine as a peroxidase substrate in microplate enzyme linked immunosorbent assay. 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Trans R","volume":"2","author":"Diamond, L.S.; Harlow, D.R.; Cunnick, C.C.","year":"1978","unstructured":"Diamond LS, Harlow DR, Cunnick CC. A new medium for the axenic cultivation of E histolytica and other Entamoebae. Trans R Soc Trop Med Hyg 1978;2:431-3.","journal-title":"Soc Trop Med Hyg"},{"key":"ref_9","first-page":"13","article-title":"Antigen fraction from Entamoeba histolytica strain HK9 for use in ELISA","volume":"3","author":"Grundy, M.S.","year":"1982","unstructured":"Grundy MS. Antigen fraction from Entamoeba histolytica strain HK9 for use in ELISA. Arch Invest Med 1982;3(Suppl 13):249-51.","journal-title":"Arch Invest Med"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"905","DOI":"10.1128\/JCM.23.5.905-910.1986","article-title":"Isolation and identification of a Giardia lamblia-specific stool antigen (GSA 65) useful in coprodiagnosis of giardiasis","volume":"23","author":"Rosoff, J.D.; Stibbs, H.H.","year":"1986","unstructured":"Rosoff JD, Stibbs HH. Isolation and identification of a Giardia lamblia-specific stool antigen (GSA 65) useful in coprodiagnosis of giardiasis. J Clin Microbiol 1986;23: 905-7.","journal-title":"J Clin Microbiol"}],"container-title":["Journal of Clinical Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1136\/jcp.43.11.950","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,13]],"date-time":"2025-12-13T04:36:14Z","timestamp":1765600574000},"score":1,"resource":{"primary":{"URL":"https:\/\/jcp.bmj.com\/lookup\/doi\/10.1136\/jcp.43.11.950"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1990,11]]},"references-count":10,"journal-issue":{"issue":"11","published-online":{"date-parts":[[1990,11,1]]},"published-print":{"date-parts":[[1990,11]]}},"alternative-id":["10.1136\/jcp.43.11.950"],"URL":"https:\/\/doi.org\/10.1136\/jcp.43.11.950","relation":{},"ISSN":["0021-9746","1472-4146"],"issn-type":[{"type":"print","value":"0021-9746"},{"type":"electronic","value":"1472-4146"}],"subject":[],"published":{"date-parts":[[1990,11]]}}}