{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,21]],"date-time":"2025-11-21T18:58:46Z","timestamp":1763751526432,"version":"3.45.0"},"reference-count":31,"publisher":"BMJ","issue":"12","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clin Pathol"],"published-print":{"date-parts":[[1990,12]]},"abstract":"<jats:p>Abnormal fucosylation of cystic fibrosis mucin was previously shown using peroxidase conjugated lectins on ileal tissue sections. These abnormally fucosylated glycoproteins were investigated further using monoclonal antibodies to fucosyl oligosaccharides based on type 1 and type 2 blood group precursor chains. The results of this study, using monoclonal antibodies to blood group glycoproteins in cystic fibrosis, were negative, yet abnormal fucosylation had been found using lectin histochemistry. Using monoclonal antibodies, lectins, and appropriate enzymes, such as glycosyl hydrolases, it should be possible to delineate further the abnormality found in glycoproteins in cystic fibrosis on appropriately fixed ileal sections, obtained from infants at term presenting with meconium ileus.<\/jats:p>","DOI":"10.1136\/jcp.43.12.1019","type":"journal-article","created":{"date-parts":[[2007,6,9]],"date-time":"2007-06-09T00:50:46Z","timestamp":1181350246000},"page":"1019-1022","source":"Crossref","is-referenced-by-count":5,"title":["Abnormal fucosylation of-ileal mucus in cystic fibrosis: II. A histochemical study using monoclonal antibodies to fucosyl oligosaccharides."],"prefix":"10.1136","volume":"43","author":[{"given":"A","family":"King","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M","family":"McLeish","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S","family":"Thiru","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[1990,12,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1203\/00006450-198301000-00013","article-title":"Human intestinal mucin in cystic fibrosis","volume":"17","author":"Wesley, A.; Forstner, J.; Qureshi, R.; Mantle, M.; Forstner, G.","year":"1983","unstructured":"Wesley A, Forstner J, Qureshi R, Mantle M, Forstner G. Human intestinal mucin in cystic fibrosis. 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Specificity of twelve lectins towards oligosaccharides and glycopeptides related to N-glycosyl proteins. Eur J Biochem 1981;117:41-55.","journal-title":"Eur J Biochem"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"3184","DOI":"10.1021\/bi00712a029","article-title":"Specificity of purified haemagglutinin (lectin) from Lotus tetragonolobus","volume":"13","author":"Pereira, M.E.A.; Kabat, E.A.","year":"1974","unstructured":"Pereira MEA, Kabat EA. Specificity of purified haemagglutinin (lectin) from Lotus tetragonolobus. Biochemistry 1974;13:3184-92.","journal-title":"Biochemistry"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1016\/0003-9861(78)90149-2","article-title":"Immunochemical studies on the combining site of the blood group H-specific lectin from Ulex europaeus seeds","volume":"185","author":"Pereira, M.E.A.; Kisailus, E.C.; Grenzof, F.; Kabat, E.A.","year":"1978","unstructured":"Pereira MEA, Kisailus EC, Grenzof F, Kabat EA. 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Distribution of blood group antigens A, B, H Lewisa and Lewisb in human normal, foetal and malignant colonic tissue. Cancer Res 1985;45:4499-51 1.","journal-title":"Cancer Res; Lewisa and Lewisb in human normal, foetal and malignant colonic tissue"},{"key":"ref_17","first-page":"4820","article-title":"Gastrointestinal cancer-associated antigen in immunoperoxidase assay","volume":"42","author":"Atkinson, B.F.; Ernst, C.S.; Herlyn, M.; Steplewski, Z.; Sears, H.F.","year":"1982","unstructured":"Atkinson BF, Ernst CS, Herlyn M, Steplewski Z, Sears HF. Gastrointestinal cancer-associated antigen in immunoperoxidase assay. 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Med Biol 1982;60:227-36.","journal-title":"Med Biol"},{"key":"ref_20","first-page":"381","article-title":"ABH secretor status in cystic fibrosis-a negative report","volume":"67","author":"Haponik, E.F.; Stokes, D.; Rosenstein, B.J.; Hughes, W.T.","year":"1985","unstructured":"Haponik EF, Stokes D, Rosenstein BJ, Hughes WT. ABH secretor status in cystic fibrosis-a negative report. Eur J Respir Dis 1985;67:381-4.","journal-title":"Eur J Respir Dis"},{"key":"ref_21","first-page":"83","article-title":"Is sulphated blood-group substance in pancreas a factor in cystic fibrosis?","volume":"86","author":"Rolla, G.; Melsen, B.; Sonju, T.","year":"1978","unstructured":"Rolla G, Melsen B, Sonju T. Is sulphated blood-group substance in pancreas a factor in cystic fibrosis? Acta Pathol Microbiol Scand 1978;(Sect A);86:83-6.","journal-title":"Acta Pathol Microbiol Scand"},{"key":"ref_22","first-page":"28","article-title":"Structure of gastrointestinal mucus glycoproteins and the viscous and gel-forming properties of mucus","volume":"34","author":"Allen, A.","year":"1978","unstructured":"Allen A. Structure of gastrointestinal mucus glycoproteins and the viscous and gel-forming properties of mucus. Br Med Bull 1978;34:28.","journal-title":"Br Med Bull"},{"issue":"95-104","key":"ref_23","first-page":"25","article-title":"Human respiratory tract secretions: mucous glycoproteins of nonpurulent tracheobronchial secretions and sputum of patients with bronchitis and cystic fibrosis","volume":"177","author":"Boat, T.F.; Cheng, P.W.; Iyer, R.N.; Carlson, D.M.; Polony, I.","year":"1976","unstructured":"Boat TF, Cheng PW, Iyer RN, Carlson DM, Polony I. Human respiratory tract secretions: mucous glycoproteins of nonpurulent tracheobronchial secretions and sputum of patients with bronchitis and cystic fibrosis. Arch Biochem Biophys 1976;177:95-104.. 25 Frates RC, Kaizu T, Last JA. Mucous glycoproteins secreted by respiratory epithelial tissue from cystic fibrosis patients. Pediatr Res 1983;17:30-4.","journal-title":"Arch Biochem Biophys; Mucous glycoproteins secreted by respiratory epithelial tissue from cystic fibrosis patients"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1172\/JCI114171","article-title":"Increased sulfation of glycoconjugates by cultured nasal epithelial cells from patients with cystic fibrosis","volume":"84","author":"Cheng, P.W.; Boat, T.F.; Cranfill, K.; Yankaskas, JR, Boucher; R.C.","year":"1989","unstructured":"Cheng PW, Boat TF, Cranfill K, Yankaskas JR, Boucher RC. Increased sulfation of glycoconjugates by cultured nasal epithelial cells from patients with cystic fibrosis. J Clin Invest 1989;84:68-72.","journal-title":"J Clin Invest"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1016\/0009-8981(83)90387-X","article-title":"The composition of a mucus glycoprotein from meconium of cystic fibrosis, healthy pre-temi and full-term neonates","volume":"135","author":"Ryley, H.C.; Rennie, D.; Bradley, D.M.","year":"1983","unstructured":"Ryley HC, Rennie D, Bradley DM. The composition of a mucus glycoprotein from meconium of cystic fibrosis, healthy pre-temi and full-term neonates. Clin Chim Acta 1983;135:49-56.","journal-title":"Clin Chim Acta"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1038\/322407a0","article-title":"Chloride ions and cystic fibrosis","volume":"322","author":"Case, M.","year":"1986","unstructured":"Case M. Chloride ions and cystic fibrosis. Nature 1986; 322:407.","journal-title":"Nature"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1203\/00006450-198703000-00005","article-title":"Immunocytochemistry of band 3 protein in kidney and other tissues of control and cystic fibrosis patients","volume":"21","author":"Hazen-Martin, D.J.; Pasternack, G.; Hennigar, R.A.; Spicer, S.S.; Sens, D.A.","year":"1987","unstructured":"Hazen-Martin DJ, Pasternack G, Hennigar RA, Spicer SS, Sens DA. Immunocytochemistry of band 3 protein in kidney and other tissues of control and cystic fibrosis patients. Pediatr Res 1987;21:235-7.","journal-title":"Pediatr Res"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1042\/bj1730333","article-title":"Blood-group-I activity associated with band 3, the major intrinsic membrane protein of human erythrocytes","volume":"173","author":"Childs, R.A.; Feizi, T.; Fukuda, M.; Hakamori, S.","year":"1978","unstructured":"Childs RA, Feizi T, Fukuda M, Hakamori S. Blood-group-I activity associated with band 3, the major intrinsic membrane protein of human erythrocytes. Biochem J 1978;173:333-6.","journal-title":"Biochem J"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"575","DOI":"10.1093\/infdis\/151.4.575","article-title":"Pulmonary disease associated with Pseudomonas aeruginosa in cystic fibrosis: current status of the host bacterium interaction","volume":"151","author":"Pier, G.B.","year":"1985","unstructured":"Pier GB. Pulmonary disease associated with Pseudomonas aeruginosa in cystic fibrosis: current status of the host bacterium interaction. J Infect Dis 1985;151:575-80.","journal-title":"J Infect Dis"},{"issue":"0","key":"ref_30","first-page":"179","article-title":"Bronchial mucus and its glycoproteins in cystic fibrosis","volume-title":"years of cysticfibrosis: collected papers","volume":"1","author":"Reid, L.","year":"1981","unstructured":"Reid L. Bronchial mucus and its glycoproteins in cystic fibrosis. In: Warwick WJ, ed. 1,000 years of cysticfibrosis: collected papers. University of Minnesota: St Paul, MN, 1981:179-83."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1059","DOI":"10.1126\/science.2772657","article-title":"Identification of the cystic fibrosis gene","volume":"245","author":"Riordan, JR, Lap-Chee Tsui; Collins, F.S.","year":"1989","unstructured":"Riordan JR, Lap-Chee Tsui, Collins FS, et al. Identification of the cystic fibrosis gene. 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