{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,14]],"date-time":"2025-12-14T06:06:00Z","timestamp":1765692360914,"version":"3.48.0"},"reference-count":35,"publisher":"BMJ","issue":"7","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clin Pathol"],"published-print":{"date-parts":[[1990,7]]},"abstract":"<jats:p>The value of genotypic analysis for routine assessment of leukaemia and lymphoma was shown by the findings in a selected series of 30 cases. T cell receptor (TcR) gene rearrangements were observed in six out of nine cases of CD3+ CD8+ lymphocytoses and provided clear evidence for clonality in this group. The T cell proliferations in two of the remaining cases masked B cell lymphocytic leukaemia and hairy cell leukaemia, while in the third case no cause was found for the polyclonal proliferation. Heterogeneity of phenotype and genotype were observed in peripheral T cell lymphomas: one out of six cases showed TcR gene rearrangement, one case retained its germline configuration, a further case masked B cell lymphoma and the remainder were polyclonal. Genotypic analysis was helpful in the analysis of a tumour of mixed T cell and myeloid phenotype which was shown to be germline for TcR and immunoglobulin genes, consistent with a myeloid origin. Two histiocytic tumours were found to have clonal rearrangement of TcR genes. Nine out of 11 B cell tumours showed immunoglobulin gene rearrangement. It is concluded that genetic analyses are useful in the analysis of T cell, histiocytic, and B cell tumours in which an immunoglobulin phenotype cannot be defined.<\/jats:p>","DOI":"10.1136\/jcp.43.7.548","type":"journal-article","created":{"date-parts":[[2007,6,9]],"date-time":"2007-06-09T00:50:46Z","timestamp":1181350246000},"page":"548-553","source":"Crossref","is-referenced-by-count":10,"title":["Clonality of T cell and phenotypically undefined lymphoid neoplasms: the value of genotypic analyses."],"prefix":"10.1136","volume":"43","author":[{"given":"E","family":"Hodges","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G N","family":"Stacey","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W M","family":"Howell","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D B","family":"Jones","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J L","family":"Smith","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[1990,7,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1093\/ajcp\/77.1.46","article-title":"Predictability of immunologic phenotype by morphological criteria in diffuse aggressive non-Hodgkin's lymphomas","volume":"77","author":"Jaffe, E.S.; Strauchen, J.A.; Berard, C.W.","year":"1982","unstructured":"Jaffe ES, Strauchen JA, Berard CW. Predictability of immunologic phenotype by morphological criteria in diffuse aggressive non-Hodgkin's lymphomas. Am J Clin Pathol 1982;77:46-9.","journal-title":"Am J Clin Pathol"},{"key":"ref_2","first-page":"1216","article-title":"Predictability of immunologic phenotype of malignant lymphomas by conventional morphology","volume":"43","author":"Frizzera, G.; Gail-Peczalska, K.J.; Bloomfield, C.D.; Kersey, J.H.","year":"1970","unstructured":"Frizzera G, Gail-Peczalska KJ, Bloomfield CD, Kersey JH. Predictability of immunologic phenotype of malignant lymphomas by conventional morphology. Cancer 1970;43: 1216-24.","journal-title":"Cancer"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1002\/hon.2900070104","article-title":"Correlation between histology and immunotype in a series of 322 cases of non-Hodgkin's lymphoma","volume":"7","author":"Smith, J.L.; Jones, D.B.; Bell, A.J.; Wright, D.H.","year":"1989","unstructured":"Smith JL, Jones DB, Bell AJ, Wright DH. Correlation between histology and immunotype in a series of 322 cases of non-Hodgkin's lymphoma. Haematol Oncol 1989;7: 37-48.","journal-title":"Haematol Oncol"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"746","DOI":"10.1177\/28.8.7003001","article-title":"Immunohistological analysis of the organization of normal lymphoid tissue and non Hodgkin's","volume":"28","author":"Stein, H.; Bonk, A.; Tolksdorf, G.; Lennert, K.; Rodt, H.; Gerdes, J.","year":"1980","unstructured":"Stein H, Bonk A, Tolksdorf G, Lennert K, Rodt H, Gerdes J. Immunohistological analysis of the organization of normal lymphoid tissue and non Hodgkin's. J Histochem Cytochem 1980;28:746-60.","journal-title":"J Histochem Cytochem"},{"key":"ref_5","first-page":"88","article-title":"B cell malignant lymphomas: an immunologic perspective","author":"Warnke, R.A.; Weiss, L.M.","year":"1987","unstructured":"Warnke RA, Weiss LM. B cell malignant lymphomas: an immunologic perspective. In: Berard CW, Dorfman RF, Kaufman N, eds. Malignant lymphoma. International Academy of Pathology Monographs 1987. Baltimore: Williams & Wilkins Co, 1987:88-103.","journal-title":"International Academy of Pathology Monographs"},{"key":"ref_6","first-page":"425","article-title":"Follicular large cell lymphoma: an inimunophenotype study","volume":"123","author":"Garcia, C.G.; Warnke, R.A.; Weiss, L.M.","year":"1986","unstructured":"Garcia CG; Warnke RA, Weiss LM. Follicular large cell lymphoma: an inimunophenotype study. Am J Pathol 1986;123:425-31.","journal-title":"Am J Pathol"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"169","DOI":"10.7326\/0003-4819-102-2-169","article-title":"Leukaemia of large granular lymphocytes: association with clonal chromosomal abnormalities and autoimmune neutropenia, thrombocytopenia and haemolytic anaemia","volume":"102","author":"Loughran, T.R.; Kadin, M.E.; Starkebaum, G.","year":"1985","unstructured":"Loughran TR, Kadin ME, Starkebaum G, et al. Leukaemia of large granular lymphocytes: association with clonal chromosomal abnormalities and autoimmune neutropenia, thrombocytopenia and haemolytic anaemia. Ann Intern Med 1985;102:169-75.","journal-title":"Ann Intern Med"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"2156","DOI":"10.1084\/jem.162.6.2156","article-title":"T cell receptor chain rearrangements in lymphoproliferative disorders of large granular lymphocytes\/natural killer cells","volume":"162","author":"Rambaldi, A.; Pelicci, P.G.; Allavena, P.","year":"1985","unstructured":"Rambaldi A, Pelicci PG, Allavena P, et al. T cell receptor chain rearrangements in lymphoproliferative disorders of large granular lymphocytes\/natural killer cells. J Exp Med 1985;162:2156-62.","journal-title":"J Exp Med"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"746","DOI":"10.1136\/jcp.41.7.746","article-title":"CD3 + CD8 + T cell lymphocytosis masking B cell leukaemia","volume":"41","author":"Smith, J.L.; Oscier, D.G.; Haegert, D.G.","year":"1988","unstructured":"Smith JL, Oscier DG, Haegert DG, et al. CD3 + CD8 + T cell lymphocytosis masking B cell leukaemia. J Clin Pathol 1988;41:746-52.","journal-title":"J Clin Pathol"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1002\/hon.2900040306","article-title":"Phenotype heterogeneity displayed by T non Hodgkin's lymphoma (T-NHL) cells dispersed from diagnostic lymph node biopsies","volume":"4","author":"Jones, D.B.; Wright, D.H.; Paul, F.; Smith, J.L.","year":"1986","unstructured":"Jones DB, Wright DH, Paul F, Smith JL. Phenotype heterogeneity displayed by T non Hodgkin's lymphoma (T-NHL) cells dispersed from diagnostic lymph node biopsies. Haematol Oncol 1986;4:219-26.","journal-title":"Haematol Oncol"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"723","DOI":"10.1038\/bjc.1988.297","article-title":"Phenotypic and genotypic heterogeneity of peripheral T cell lymphoma","volume":"58","author":"Smith, J.L.; Haegert, D.G.; Hodges, E.","year":"1988","unstructured":"Smith JL, Haegert DG, Hodges E, et al. Phenotypic and genotypic heterogeneity of peripheral T cell lymphoma. Br J Cancer 1988;58:723-9.","journal-title":"Br J Cancer"},{"key":"ref_12","article-title":"Immunological investigation of lymphoid neoplasms. In: Practical methods in clinical immunology","author":"Stevenson, G.T.; Smith, J.L.; Hamblin, T.J.","year":"1983","unstructured":"Stevenson GT, Smith JL, Hamblin TJ. Immunological investigation of lymphoid neoplasms. In: Practical methods in clinical immunology. Edinburgh: Churchill Livingstone, 1983."},{"issue":"3","key":"ref_13","first-page":"603","article-title":"Updated Kiel classification for lymphomas","volume":"292","author":"Stansfield, A.G.; Diebold, J.; Kapanci, Y.","year":"1988","unstructured":"Stansfield AG, Diebold J, Kapanci Y, et al. Updated Kiel classification for lymphomas. 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Adv Cancer Res 1984;42:67-147.","journal-title":"Adv Cancer Res"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1111","DOI":"10.1016\/0161-5890(86)90009-X","article-title":"HLA-DR allogenotyping using exon specific cDNA probes and application of rapid minigel methods","volume":"23","author":"Bidwell, J.L.; Jarrold, E.A.","year":"1986","unstructured":"Bidwell JL, Jarrold EA. HLA-DR allogenotyping using exon specific cDNA probes and application of rapid minigel methods. Mol Immunol 1986;23: 1111-6.","journal-title":"Mol Immunol"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"503","DOI":"10.1016\/S0022-2836(75)80083-0","article-title":"Detection of specific sequences among DNA fragments separated by gel electrophoresis","volume":"98","author":"Southern, E.M.","year":"1975","unstructured":"Southern EM. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 1975;98:503-17.","journal-title":"J Mol Biol"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"655","DOI":"10.1002\/j.1460-2075.1982.tb01223.x","article-title":"The sequence of a human immunoglobulin episilon heavy chain constant region gene and evidence for three non-allelic genes","volume":"1","author":"Flanagan, J.G.; Rabbitts, T.H.","year":"1982","unstructured":"Flanagan JG, Rabbitts TH. The sequence of a human immunoglobulin episilon heavy chain constant region gene and evidence for three non-allelic genes. EMBO J 1982;1:655-60.","journal-title":"EMBO J"},{"key":"ref_19","first-page":"63","article-title":"Rearrangements of immunoglobulin genes correlate with phenotypic markers in B cell malignancies","volume":"2","author":"Foroni, L.; Catovsky, D.; Rabbitts, T.H.; Luzzato, L.","year":"1984","unstructured":"Foroni L, Catovsky D, Rabbitts TH, Luzzato L. Rearrangements of immunoglobulin genes correlate with phenotypic markers in B cell malignancies. Mol Biol Med 1984;2:63-79.","journal-title":"Mol Biol Med"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"521","DOI":"10.1038\/312521a0","article-title":"Sequence and expression of transcripts of the human T-cell receptor f chain genes","volume":"312","author":"Yoshikai, Y.; Anatoniou, D.; Clark, S.P.","year":"1984","unstructured":"Yoshikai Y, Anatoniou D, Clark SP, et al. Sequence and expression of transcripts of the human T-cell receptor f chain genes. Nature 1984;312:521-4.","journal-title":"Nature"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"464","DOI":"10.1038\/316464a0","article-title":"Two tandemly organised human genes encoding the T cell y constant region sequences show multiple rearrangement in different Tcell types","volume":"316","author":"LeFranc, M.-P.; Rabbitts, T.H.","year":"1985","unstructured":"LeFranc M-P, Rabbitts TH. Two tandemly organised human genes encoding the T cell y constant region sequences show multiple rearrangement in different Tcell types. Nature 1985;316:464-6.","journal-title":"Nature"},{"key":"ref_22","first-page":"385","article-title":"The mechanism of chromosomal translocation","volume":"7","author":"Boehm, T.; Baer, R.; Lavenir, I.","year":"1988","unstructured":"Boehm T, Baer R, Lavenir I, et al. The mechanism of chromosomal translocation t (11:14) involving the T-cell receptor of Co locus on human chromosome 14ql 1 and a transcribed region of chromosome Ipl5. EMBO J 1988;7:385-94.","journal-title":"EMBO J; involving the T-cell receptor of Co locus on human chromosome 14ql 1 and a transcribed region of chromosome Ipl5"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1661","DOI":"10.1002\/j.1460-2075.1988.tb02993.x","article-title":"Complex rearrangements within the human Jo-Co\/Ja-Ca locus and aberrant recombination between Ja segments","volume":"7","author":"Baer, R.; B, oehm T.; Yssel, H.; Spits, H.; Rabbitts, T.H.","year":"1988","unstructured":"Baer R, B,oehm T, Yssel H, Spits H, Rabbitts TH. Complex rearrangements within the human Jo-Co\/Ja-Ca locus and aberrant recombination between Ja segments. EMBO J 1988;7:1661-8.","journal-title":"EMBO J"},{"key":"ref_24","first-page":"266","article-title":"A technique for radiolabelling DNA restriction endonuclease fragments to high specific activity","volume":"137","author":"Feinberg, A.P.; Vogelstein, B.","year":"1985","unstructured":"Feinberg AP, Vogelstein B. A technique for radiolabelling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 1985;137:266-7.","journal-title":"Anal Biochem"},{"key":"ref_25","first-page":"1500","article-title":"') gene rearrangements and expression in normal and leukaemic large granular lymphocytes\/natural killer cells","volume":"70","author":"Pellicci, P.-G.; Allavena, P.; Subar, M.","year":"1987","unstructured":"Pellicci P-G, Allavena P, Subar M, et al. T cell receptor (a, f, ') gene rearrangements and expression in normal and leukaemic large granular lymphocytes\/natural killer cells. Blood 1987;70:1500-8.","journal-title":"Blood"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1618","DOI":"10.4049\/jimmunol.138.5.1618","article-title":"Limited diversity and selection of rearranged y genes in polyclonal T cells","volume":"138","author":"Uppenkamp, M.; Pittaluga, S.; Lipford, E.; Cossman, J.","year":"1987","unstructured":"Uppenkamp M, Pittaluga S, Lipford E, Cossman J. Limited diversity and selection of rearranged y genes in polyclonal T cells. J Immunol 1987;138:1618-20.","journal-title":"J Immunol"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1002\/eji.1830180126","article-title":"Non random TcRy variable gene rearrangement in normal human T cells and T cell leukaemias","volume":"18","author":"Migone, N.; Casarati, G.; DiCelle, P.F.; Lusso, P.; Foa, R.; Lefranc, M.","year":"1988","unstructured":"Migone N, Casarati G, DiCelle PF, Lusso P, Foa R, Lefranc M. Non random TcRy variable gene rearrangement in normal human T cells and T cell leukaemias. Eur J Immunol 1988;18:173-8.","journal-title":"Eur J Immunol"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1002\/ajh.2830290206","article-title":"Association of large granular lymphocyte\/ natural killer cell proliferative disease and second haematologic malignancy","volume":"29","author":"Bassan, R.; Rambaldi, A.; Allavena, P.; Abbate, M.; Marini, B.; Barbui, T.","year":"1988","unstructured":"Bassan R, Rambaldi A, Allavena P, Abbate M, Marini B, Barbui T. Association of large granular lymphocyte\/ natural killer cell proliferative disease and second haematologic malignancy. Am JHaematol 1988;29:85-93.","journal-title":"Am JHaematol"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"579","DOI":"10.1182\/blood.V72.2.579.579","article-title":"Acute myeloid leukaemia with T lymphoid features: a distinct biologic and clinical entity","volume":"72","author":"Cross, A.H.; Goorma, R.M.; Nuss, R.","year":"1988","unstructured":"Cross AH, Goorma RM, Nuss R, et al. Acute myeloid leukaemia with T lymphoid features: a distinct biologic and clinical entity. Blood 1988;72:579-87.","journal-title":"Blood"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"381","DOI":"10.1182\/blood.V73.2.381.381","article-title":"CD7 and CD4 - CD8 - acute leukaemia: A syndrome of malignant pluripotent lymphohaematopoietic cells","volume":"73","author":"Kurtzberg, J.; Waldmann, T.A.; Davey, M.P.","year":"1989","unstructured":"Kurtzberg J, Waldmann TA, Davey MP, et al. CD7 and CD4 - CD8 - acute leukaemia: A syndrome of malignant pluripotent lymphohaematopoietic cells. Blood 1989; 73:381-90.","journal-title":"Blood"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1593","DOI":"10.1056\/NEJM198312293092601","article-title":"Immunoglobulin gene rearrangement as unique clonal markers in human lymphoid neoplasms","volume":"309","author":"Arnold, A.; Crossman, J.; Bakhshi, A.; Jaffe, E.S.; Waldmann, T.A.; Korsmeyer, S.J.","year":"1983","unstructured":"Arnold A, Crossman J, Bakhshi A, Jaffe ES, Waldmann TA, Korsmeyer SJ. Immunoglobulin gene rearrangement as unique clonal markers in human lymphoid neoplasms. N EnglJ Med 1983;309:1593-9.","journal-title":"N EnglJ Med"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"980","DOI":"10.1182\/blood.V66.4.980.980","article-title":"Peripheral T cell lymphoma: Immunologic and cell kinetic observations associated with morphologic progression","volume":"66","author":"Winberg, C.D.; Sheibani, K.; Krance, R.; Rappaport, H.","year":"1985","unstructured":"Winberg CD, Sheibani K, Krance R, Rappaport H. Peripheral T cell lymphoma: Immunologic and cell kinetic observations associated with morphologic progression. Blood 1985;66:980-9.","journal-title":"Blood"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1183","DOI":"10.1001\/archderm.1987.01660330094017","article-title":"TcR gene rearrangements in regressing atypical histiocytosis","volume":"123","author":"Headington, J.T.; Roth, M.S.; Ginsberg, D.; Lichter, A.S.; Hyder, D.; Schnitzer, B.","year":"1987","unstructured":"Headington JT, Roth MS, Ginsberg D, Lichter AS, Hyder D, Schnitzer B. TcR gene rearrangements in regressing atypical histiocytosis. Arch Dermatol 1987;123:1183-7.","journal-title":"Arch Dermatol"},{"key":"ref_34","first-page":"18","article-title":"in B cell lymphomas as a cause for defective immunoglobulin production","volume":"169","author":"de Jong D; BMH, Voefdijk; GJB, Van Omman; JC, KluinNelemans; GC, Beverstock; PHM, Kluin","year":"1989","unstructured":"de Jong D, Voefdijk BMH, Van Omman GJB, KluinNelemans JC, Beverstock GC, Kluin PHM. Translocation + (14:18) in B cell lymphomas as a cause for defective immunoglobulin production. J Exp Med 1989; 169:613-24.","journal-title":"J Exp Med"},{"issue":"27","key":"ref_35","first-page":"147","article-title":"Diffuse B cell lymphomas with T cell predominance in patients with follicular lymphoma or pseudo-T cell lymphoma. Lab Invest","volume":"50","author":"Jaffe, E.S.; Longo, D.L.; Cossman, J.; Hsu, S.M.; Korsmeyer, S.J.","year":"1984","unstructured":"Jaffe ES, Longo DL, Cossman J, Hsu SM, Korsmeyer SJ. Diffuse B cell lymphomas with T cell predominance in patients with follicular lymphoma or pseudo-T cell lymphoma. Lab Invest 1984;50:27A-28A. 38 Brada M, Mizutani S, Molgaard H. Circulating lymphoma cells in patients with B and T non-Hodgkin's lymphoma detected by immunoglobulin and T cell receptor gene rearrangement. Br J Cancer 1987;56:147-52.","journal-title":"Br J Cancer; Circulating lymphoma cells in patients with B and T non-Hodgkin's lymphoma detected by immunoglobulin and T cell receptor gene rearrangement"}],"container-title":["Journal of Clinical Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1136\/jcp.43.7.548","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,14]],"date-time":"2025-12-14T06:04:04Z","timestamp":1765692244000},"score":1,"resource":{"primary":{"URL":"https:\/\/jcp.bmj.com\/lookup\/doi\/10.1136\/jcp.43.7.548"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1990,7]]},"references-count":35,"journal-issue":{"issue":"7","published-online":{"date-parts":[[1990,7,1]]},"published-print":{"date-parts":[[1990,7]]}},"alternative-id":["10.1136\/jcp.43.7.548"],"URL":"https:\/\/doi.org\/10.1136\/jcp.43.7.548","relation":{},"ISSN":["0021-9746","1472-4146"],"issn-type":[{"type":"print","value":"0021-9746"},{"type":"electronic","value":"1472-4146"}],"subject":[],"published":{"date-parts":[[1990,7]]}}}