{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,14]],"date-time":"2025-12-14T09:40:25Z","timestamp":1765705225658,"version":"3.48.0"},"reference-count":16,"publisher":"BMJ","issue":"8","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clin Pathol"],"published-print":{"date-parts":[[1990,8]]},"abstract":"<jats:p>Several immunohistological methods for the demonstration of progesterone receptors were tried on routinely processed paraffin wax sections of breast carcinoma, using Abbott's PgR-ICA monoclonal antibody. The best results were obtained with the avidin-biotin-immunoperoxidase complex method with no prior trypsinisation or DNAse digestion, and with imidazole added to the final diaminobenzidine developing solution. A simple semiquantitative scoring system was used to assess the staining results which were then compared with the results obtained by a standard dextran-coated charcoal biochemical assay. Of 31 cases examined, the results of the two methods were concordant in 25 (81%) of cases. This is near the higher end of the concordance range obtained by several other authors using frozen sections. The discordance encountered in a few cases was possibly the result of sampling errors which are more likely to occur with the chemical rather than the histological method. It is concluded that the method described here is fairly reliable and would greatly simplify the process of assessment of progesterone receptors in breast, and possibly other tumours.<\/jats:p>","DOI":"10.1136\/jcp.43.8.671","type":"journal-article","created":{"date-parts":[[2007,6,9]],"date-time":"2007-06-09T00:50:46Z","timestamp":1181350246000},"page":"671-674","source":"Crossref","is-referenced-by-count":12,"title":["Demonstration of progesterone receptors in paraffin wax sections of breast carcinoma."],"prefix":"10.1136","volume":"43","author":[{"given":"S","family":"Soomro","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S","family":"Shousha","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[1990,8,1]]},"reference":[{"key":"ref_1","first-page":"55","article-title":"The measurement of receptors for oestradiol and progesterone in human breast tumours","volume-title":"receptor assays in human breast tumours. Methodological and clinical aspects","author":"King, R.J.B.; Redgrave, S.; Hayward, J.L.; Millis, R.R.; Rubens, R.D.","year":"1979","unstructured":"King RJB, Redgrave S, Hayward JL, Millis RR, Rubens RD. The measurement of receptors for oestradiol and progesterone in human breast tumours. In: King RJB, ed. Steroid receptor assays in human breast tumours. Methodological and clinical aspects. Cardiff: Alpha Omega, 1979:55-72."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1136\/jcp.43.3.239","article-title":"Immunohistological study ofoestrogen receptors in breast carcinomas that are biochemically receptor negative: an investigation of 60 cases using paraffin sections","volume":"43","author":"Shousha, S.; Stamp, T.; James, K.R.; Alaghband-Zadeh","year":"1990","unstructured":"Shousha S, Stamp T, James KR, Alaghband-Zadeh. Immunohistological study ofoestrogen receptors in breast carcinomas that are biochemically receptor negative: an investigation of 60 cases using paraffin sections. J Clin Pathol 1990;43:239-42.","journal-title":"J Clin Pathol"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1177\/30.5.6176617","article-title":"Imidazole increases the sensitivity of the cytochemical reaction for peroxidase with diaminobenzidine at a neutral pH","volume":"30","author":"Straus, W.","year":"1982","unstructured":"Straus W. Imidazole increases the sensitivity of the cytochemical reaction for peroxidase with diaminobenzidine at a neutral pH. 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Lancet 1989;ii:40-1.","journal-title":"Lancet"},{"key":"ref_8","first-page":"457","article-title":"Immunocytochemical staining of progesterone receptor in paraffin sections of human breast cancers","volume":"135","author":"Perrot-Applanat, M.; Groyer-Picard, M.-T.; Hai, M.T.V.; Pallud, C.; Spyratos, F.; Milgrom, E.","year":"1989","unstructured":"Perrot-Applanat M, Groyer-Picard M-T, Hai MTV, Pallud C, Spyratos F, Milgrom E. Immunocytochemical staining of progesterone receptor in paraffin sections of human breast cancers. Am J Pathol 1989;135:457-68.","journal-title":"Am J Pathol"},{"key":"ref_9","first-page":"1147","article-title":"Dapunt 0. Monoclonal technique to aid decision on endocrine therapy in breast cancer","volume":"i:","author":"Muller-Holzner, E.; Zeimet, A.; Muller, L.C.; Daxenbichler, G.","year":"1989","unstructured":"Muller-Holzner E, Zeimet A, Muller LC, Daxenbichler G, Dapunt 0. Monoclonal technique to aid decision on endocrine therapy in breast cancer. 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Immunocytochemical detection ofestrogen and progesterone receptors in 124 human breast cancers. Am J Clin Pathol 1988; 89:137-42.","journal-title":"Am J Clin Pathol"},{"key":"ref_12","first-page":"2652","article-title":"Immunocytochemical study with monoclonal antibodies to progesterone receptor in human breast tumours","volume":"47","author":"Perrot-Applanat, M.; Groyer-Picard, M.-T.; Lorenzo, F.","year":"1987","unstructured":"Perrot-Applanat M, Groyer-Picard M-T, Lorenzo F, et al. Immunocytochemical study with monoclonal antibodies to progesterone receptor in human breast tumours. Cancer Res 1987;47:2652-61.","journal-title":"Cancer Res"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"444","DOI":"10.1136\/jcp.41.4.444","article-title":"Immunohistological demonstration of progesterone receptor in breast carcinoma: correlation with radioligand binding assays and oestrogen receptor immunohistology","volume":"41","author":"Giri, D.D.; Goepel, JR, Rogers; K, Underwood; J.C.E.","year":"1988","unstructured":"Giri DD, Goepel JR, Rogers K, Underwood JCE. Immunohistological demonstration of progesterone receptor in breast carcinoma: correlation with radioligand binding assays and oestrogen receptor immunohistology. 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Immunohistochemical determination ofestrogen and progesterone receptors in human breast carcinoma. Correlation with histopathology and DNA flow cytometry. Cancer 1989; 63:1761-7.","journal-title":"Cancer"},{"key":"ref_16","first-page":"1","article-title":"Multiparametric analysis","volume":"132","author":"Charpin, C.; Jacquemier, J.; Andrac, L.","year":"1988","unstructured":"Charpin C, Jacquemier J, Andrac L, et al. Multiparametric analysis (SAMBA 200) of the progesterone receptor immunocytochemical assay in nonmaligant and malignant breast disorders. 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