{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T19:46:31Z","timestamp":1791315991251,"version":"4.3.1"},"reference-count":27,"publisher":"BMJ","issue":"9","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clin Pathol"],"published-print":{"date-parts":[[1990,9]]},"abstract":"<jats:p>A new monoclonal antibody, JC70, raised against a membrane preparation from a spleen affected by hairy cell leukaemia, recognises a membrane bound glycoprotein identical with that of the CD31 group of monoclonal antibodies. The antibody stains a fixation resistant epitope on endothelial cells in benign and malignant conditions in a wide variety of paraffin wax embedded tissue. JC70 stained malignant endothelial cells in 10 angiosarcomas with more consistency than monoclonal or polyclonal antibodies to factor VIII related antigen (FVIII-Rag). In four cases of Kaposi's sarcoma the antibody stained malignant endothelial cells but not spindle cells. It is concluded that antibody JC70 is of value for studying benign and malignant human vascular disorders in routinely processed tissue.<\/jats:p>","DOI":"10.1136\/jcp.43.9.752","type":"journal-article","created":{"date-parts":[[2007,6,9]],"date-time":"2007-06-09T00:50:46Z","timestamp":1181350246000},"page":"752-757","source":"Crossref","is-referenced-by-count":297,"title":["JC70: a new monoclonal antibody that detects vascular endothelium associated antigen on routinely processed tissue sections."],"prefix":"10.1136","volume":"43","author":[{"given":"D V","family":"Parums","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J L","family":"Cordell","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K","family":"Micklem","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A R","family":"Heryet","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K C","family":"Gatter","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D Y","family":"Mason","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"239","published-online":{"date-parts":[[1990,9,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1093\/ajcp\/92.2.241","article-title":"The complexity of endothelial cells","volume":"92","author":"Fajardo, L.F.","year":"1989","unstructured":"Fajardo LF. The complexity of endothelial cells. Am J Clin Pathol 1989;92:241-50.","journal-title":"Am J Clin Pathol"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1093\/ajcp\/75.2.167","article-title":"Immunohistochemical identification of factor VIII related antigen in vascular endothelial cells of cutaneous lesions of alleged vascular nature","volume":"75","author":"Burgdorf, W.H.C.; Mukai, K.; Rosai, J.","year":"1981","unstructured":"Burgdorf WHC, Mukai K, Rosai J. Immunohistochemical identification of factor VIII related antigen in vascular endothelial cells of cutaneous lesions of alleged vascular nature. Am J Clin Pathol 1981;75:167-71.","journal-title":"Am J Clin Pathol"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1002\/path.1711470210","article-title":"Monoclonal antibodies to blood group isoantigens: an alternative marker to factor VIII related antigen for benign and malignant vascular endothelial cells","volume":"147","author":"Stephenson, T.J.; Mills, P.M.","year":"1985","unstructured":"Stephenson TJ, Mills PM. Monoclonal antibodies to blood group isoantigens: an alternative marker to factor VIII related antigen for benign and malignant vascular endothelial cells. JPathol 1985;147:139-48.","journal-title":"JPathol"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1002\/path.1711490203","article-title":"Endothelial cell markers in vascular neoplasms: an immunohistochemical study comparing factor VIIIrelated antigen, blood group specific antigens, 6-ketoPGF1 alpha and Ulex europaeus I lectin","volume":"149","author":"Little, D.; Said, J.W.; Siegel, R.L.; Fealy, M.; Fishbein, M.C.","year":"1986","unstructured":"Little D, Said JW, Siegel RL, Fealy M, Fishbein MC. Endothelial cell markers in vascular neoplasms: an immunohistochemical study comparing factor VIIIrelated antigen, blood group specific antigens, 6-ketoPGF1 alpha and Ulex europaeus I lectin. J Pathol 1986; 149:89-95.","journal-title":"J Pathol"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1093\/ajcp\/79.1.32","article-title":"Ulex europaeus I lectin as a marker for tumours derived from endothelial cells","volume":"79","year":"1983","unstructured":"Miettenen M, Holthofer H, Lehto VP, Virtanen MD. Ulex europaeus I lectin as a marker for tumours derived from endothelial cells Am J Clin Pathol 1983;79:32-6.","journal-title":"Am J Clin Pathol"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"463","DOI":"10.1093\/ajcp\/89.4.463","article-title":"Immunocytochemistry of angiosarcomas. A study of 19 cases with special emphasis on the applicability of endothelial cell specific markers to routinely prepared tissues","volume":"89","author":"Alles, J.U.; Bosslet, K.","year":"1988","unstructured":"Alles JU, Bosslet K. Immunocytochemistry of angiosarcomas. A study of 19 cases with special emphasis on the applicability of endothelial cell specific markers to routinely prepared tissues. Am J Clin Pathol 1988;89: 463-71.","journal-title":"Am J Clin Pathol"},{"key":"ref_7","first-page":"19","article-title":"Binding of Ulex europaeus agglutinin I in polyposis coli: comparative study with solitary adenoma in the sigmoid colon and rectum","volume":"71","author":"Yonezawa, S.; Nakamura, T.; Tanaka, S.; Maruta, K.; Nishi, M.; Sato, E.","year":"1983","unstructured":"Yonezawa S, Nakamura T, Tanaka S, Maruta K, Nishi M, Sato E. Binding of Ulex europaeus agglutinin I in polyposis coli: comparative study with solitary adenoma in the sigmoid colon and rectum. JINCI 1983;71:19-25.","journal-title":"JINCI"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1016\/0005-2736(78)90190-6","article-title":"Glvcoproteins and antigens of membranes prepared from rat thymocvtes after lysis by shearing or with detergent Tween-40","volume":"508","author":"Standring, H.; Williams, A.F.","year":"1978","unstructured":"Standring H, Williams, AF. Glvcoproteins and antigens of membranes prepared from rat thymocvtes after lysis by shearing or with detergent Tween-40. Biochim Biophys Acta 1978;508:85-92.","journal-title":"Biochim Biophys Acta"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1177\/32.2.6198355","article-title":"Immunoenzymatic labelling of monoclonal antibodies using immune complexes of alkaline phosphatase and monoclonal antialkaline phosphatase (APAAP complexes)","volume":"32","author":"Cordell, J.L.; Falini, B.; Erber, W.N.","year":"1984","unstructured":"Cordell JL, Falini B, Erber WN, et al. Immunoenzymatic labelling of monoclonal antibodies using immune complexes of alkaline phosphatase and monoclonal antialkaline phosphatase (APAAP complexes). J Histochem Cytochem 1984;32:219-29.","journal-title":"J Histochem Cytochem"},{"key":"ref_10","first-page":"35","article-title":"The use of monoclonal antibodies in histopathological diagnosis","volume-title":"Recent advances in histopathology. 12","author":"Gatter, K.C.; Falini, B.; Mason, D.Y.","year":"1984","unstructured":"Gatter KC, Falini B, Mason DY. The use of monoclonal antibodies in histopathological diagnosis. In: Anthony PP, MacSween RNM, eds. Recent advances in histopathology. 12. Edinburgh: Churchill Livingstone, 1984:35-67."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1038\/227680a0","article-title":"Cleavage of structural proteins during the assembly of the head of bacteriophage T4","volume":"227","author":"Laemmli, U.K.","year":"1970","unstructured":"Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970; 227:680-5.","journal-title":"Nature"},{"key":"ref_12","first-page":"175","article-title":"Production of monoclonal antibodies for immunocytochemical use","volume-title":"in immunocytochemistry","author":"Mason, D.Y.; Cordell, J.; Pulford, K.A.F.","year":"1983","unstructured":"Mason DY, Cordell J, Pulford KAF. Production of monoclonal antibodies for immunocytochemical use. In: Bullock GR, Petrusz P, eds. Techniques in immunocytochemistry. London, Academic Press, 1983:175."},{"key":"ref_13","first-page":"244","article-title":"The multitumour (sausage) tissue block: Novel method for immunohistochemical antibody testing","volume":"55","author":"Battifora, H.","year":"1986","unstructured":"Battifora H. The multitumour (sausage) tissue block: Novel method for immunohistochemical antibody testing. Lab Invest 1986;55:244-8.","journal-title":"Lab Invest"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1016\/0022-1759(82)90221-6","article-title":"The value of immunohistological screening in the production of monoclonal antibodies","volume":"50","author":"Naiem, M.; Gerdes, J.; Abdulaziz, Z.","year":"1982","unstructured":"Naiem M, Gerdes J, Abdulaziz Z, et al. The value of immunohistological screening in the production of monoclonal antibodies. J Immunol Methods 1982;50:145-60.","journal-title":"J Immunol Methods"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1002\/path.1711460104","article-title":"An immunohistological study of factor VIII related antigen and Kaposi's sarcoma using polyclonal and monoclonal antibodies","volume":"146","author":"Millard, P.R.; Heryet, A.R.","year":"1985","unstructured":"Millard PR, Heryet AR. An immunohistological study of factor VIII related antigen and Kaposi's sarcoma using polyclonal and monoclonal antibodies. J Pathol 1985;146: 31-8.","journal-title":"J Pathol"},{"key":"ref_16","first-page":"47","article-title":"Ulex europaeus I lectin as a marker for vascular endothelium in human tissues","author":"Holthofer, H.; Virtanen, M.D.; Kariniemi, L.; Hormia, M.; Linder, E.; Miettinen, H.","year":"1982","unstructured":"Holthofer H, Virtanen MD, Kariniemi L, Hormia M, Linder E, Miettinen H. Ulex europaeus I lectin as a marker for vascular endothelium in human tissues. Lab Invest 1982;47-60-6.","journal-title":"Lab Invest"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1097\/00000478-198006000-00008","article-title":"Localisation of FVIIIRag in vascular endothelial cells using an immunoperoxidase method","volume":"4","author":"Mukai, K.; Rosai, J.; BurgdorfWHC","year":"1980","unstructured":"Mukai K, Rosai J, BurgdorfWHC. Localisation of FVIIIRag in vascular endothelial cells using an immunoperoxidase method. Am J Surg Pathol 1980;4:273-6.","journal-title":"Am J Surg Pathol"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"569","DOI":"10.1093\/ajcp\/87.5.569","article-title":"Endothelial cell phenotypic diversity","volume":"87","author":"Turner, R.R.; Beckstead, J.H.; Warnke, R.A.","year":"1987","unstructured":"Turner RR, Beckstead JH, Warnke RA, et al. Endothelial cell phenotypic diversity. Am J Clin Pathol 1987;87: 569-75.","journal-title":"Am J Clin Pathol"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"262","DOI":"10.1136\/jcp.40.3.262","article-title":"Factor VIII related antigen positive macrophages and acquired immunodeficiency syndrome (AIDS): a problem of antibody specificity","volume":"40","author":"Millard, P.R.; Chaplin, A.J.; Heryet, A.R.; McDougall, A.C.","year":"1987","unstructured":"Millard PR, Chaplin AJ, Heryet AR, McDougall AC. Factor VIII related antigen positive macrophages and acquired immunodeficiency syndrome (AIDS): a problem of antibody specificity. J Clin Pathol 1987;40:262-6.","journal-title":"J Clin Pathol"},{"key":"ref_20","first-page":"129","article-title":"Comparison of Ulex europaeus lectin and factor VIII-related antigen in vascular endothelial cells using the immunoperoxidase method","volume":"108","author":"Ordonez, N.G.; Batsakis, J.G.","year":"1984","unstructured":"Ordonez NG, Batsakis JG. Comparison of Ulex europaeus lectin and factor VIII-related antigen in vascular endothelial cells using the immunoperoxidase method. Arch Pathol Lab Med 1984;108:129-32.","journal-title":"Arch Pathol Lab Med"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1007\/BF00430685","article-title":"Blood group antigens in vascular tumours","volume":"388","author":"Berry, C.L.; Amerigo, J.","year":"1980","unstructured":"Berry CL, Amerigo J. Blood group antigens in vascular tumours. Virchows Arch (Pathol Anat) 1980;388:167-74.","journal-title":"Virchows Arch (Pathol Anat)"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"9933","DOI":"10.1016\/S0021-9258(19)83607-4","article-title":"Laminin-a glycoprotein from basement membranes","volume":"254","author":"Timpl, R.; Rohde, H.; Robey, P.G.; Rennard, S.I.; Foidart, J.M.; Martin, G.R.","year":"1979","unstructured":"Timpl R, Rohde H, Robey PG, Rennard SI, Foidart JM, Martin GR. Laminin-a glycoprotein from basement membranes. J Biol Chem 1979;254:9933-7.","journal-title":"J Biol Chem"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1161\/01.ATV.3.5.403","article-title":"Monoclonal antibodies to E92, an endothelial cell surface antigen","volume":"3","author":"Kaplan, K.L.; Weber, D.; Cook, P.","year":"1983","unstructured":"Kaplan KL, Weber D, Cook P. Monoclonal antibodies to E92, an endothelial cell surface antigen. Arteriosclerosis 1983;3:403-12.","journal-title":"Arteriosclerosis"},{"key":"ref_24","first-page":"71","article-title":"Monoclonal antibody PAL-E specific for endothelium","volume":"52","author":"Schlingemann, R.O.; Dingjan, G.M.; Emeiss, J.J.; Blok, J.; Warnaar, S.O.; Ruiter, D.J.","year":"1985","unstructured":"Schlingemann RO, Dingjan GM, Emeiss JJ, Blok J, Warnaar SO, Ruiter DJ. Monoclonal antibody PAL-E specific for endothelium. Lab Invest 1985;52:71-6.","journal-title":"Lab Invest"},{"key":"ref_25","first-page":"735","article-title":"Histogenesis of cardiac myxomas","volume":"113","author":"Johansson, L.","year":"1989","unstructured":"Johansson L. Histogenesis of cardiac myxomas. Arch Pathol Lab Med 1989;113:735-41.","journal-title":"Arch Pathol Lab Med"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1111\/j.1365-2559.1989.tb02142.x","article-title":"Hepatic sinusoidal endothelium: Ulex lectin binding","volume":"14","author":"Petrovic, L.M.; Burroughs, A.; Scheuer, P.J.","year":"1989","unstructured":"Petrovic LM, Burroughs A, Scheuer PJ. Hepatic sinusoidal endothelium: Ulex lectin binding. Histopathology 1989; 14:233-43.","journal-title":"Histopathology"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"742","DOI":"10.1136\/jcp.39.7.742","article-title":"The histogenesis of Kaposi's sarcoma in patients with and without AIDS","volume":"39","author":"Jones, R.R.; Spaull, J.; Spry, C.; Jones, E.W.","year":"1986","unstructured":"Jones RR, Spaull J, Spry C, Jones EW. The histogenesis of Kaposi's sarcoma in patients with and without AIDS. J Clin Pathol 1986;39:742-9.","journal-title":"J Clin Pathol"}],"container-title":["Journal of Clinical Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1136\/jcp.43.9.752","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,7]],"date-time":"2025-12-07T02:27:46Z","timestamp":1765074466000},"score":1,"resource":{"primary":{"URL":"https:\/\/jcp.bmj.com\/lookup\/doi\/10.1136\/jcp.43.9.752"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1990,9]]},"references-count":27,"journal-issue":{"issue":"9","published-online":{"date-parts":[[1990,9,1]]},"published-print":{"date-parts":[[1990,9]]}},"alternative-id":["10.1136\/jcp.43.9.752"],"URL":"https:\/\/doi.org\/10.1136\/jcp.43.9.752","relation":{},"ISSN":["0021-9746","1472-4146"],"issn-type":[{"value":"0021-9746","type":"print"},{"value":"1472-4146","type":"electronic"}],"subject":[],"published":{"date-parts":[[1990,9]]}}}