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The low level of background staining combined with precise substrate deposition of the amplified peroxidase system gives higher sensitivity and resolution. This permits localisation of closely adjacent chromosomal loci in interphase nuclei. The most sensitive peroxidase based digoxigenin detection system visualises two and a half to 12 copies of human papillomavirus (HPV) per nucleus. This system is also suitable for the analysis of low copy number HPV infection of cervical tissues.<\/jats:p>","DOI":"10.1136\/jcp.44.1.33","type":"journal-article","created":{"date-parts":[[2007,6,9]],"date-time":"2007-06-09T00:50:46Z","timestamp":1181350246000},"page":"33-38","source":"Crossref","is-referenced-by-count":54,"title":["Interphase cytogenetics using biotin and digoxigenin labelled probes: III. Increased sensitivity and flexibility for detecting HPV in cervical biopsy specimens and cell lines."],"prefix":"10.1136","volume":"44","author":[{"given":"C S","family":"Herrington","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A K","family":"Graham","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J O","family":"McGee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[1991,1,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1085","DOI":"10.1136\/jcp.38.10.1085","article-title":"Sensitive system for visualising biotinylated DNA probes hybridised in situ: rapid sex determination in intact cells","volume":"38","author":"Burns, J.; Chan, V.T.-W.; Jonasson, J.A.; Fleming, K.A.; Taylor, S.; JO'D, McGee","year":"1985","unstructured":"Burns J, Chan VT-W, Jonasson JA, Fleming KA, Taylor S, McGee JO'D. 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