{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,16]],"date-time":"2026-05-16T15:37:43Z","timestamp":1778945863207,"version":"3.51.4"},"reference-count":16,"publisher":"BMJ","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clin Pathol"],"published-print":{"date-parts":[[1991,6]]},"abstract":"<jats:p>Two strains of Bacillus pumilus (M11 and M38) and one strain each of Bacillus cereus (M27), Bacillus subtilis (M67), and Enterobacter agglomerans (M14) were identified from the air of Lancashire cotton mills. These strains were tested for cytopathic effects in Vero cells; B pumilus and B cereus strains were also examined for haemolytic activity, lecithinase production, and proteolytic action on casein. Rounding and clumping of the Vero cells occurred after the addition of supernatants prepared from B pumilus and B cereus strains; finger-like projections developed in the cells treated with B pumilus supernatants. Minimal effects occurred with B subtilis and E agglomerans. After two hours of exposure B pumilus (M11) produced the greatest effect, but treatment with trypan blue showed that most cells exposed to the M11 strain were still alive after 96 hours of exposure; those exposed to the supernatant prepared from the M38 strain of B pumilus were dead. Sheep erythrocytes were lysed more rapidly by B cereus than by B pumilus, B cereus (strongly positive) had a greater effect on lecithin than either of the B pumilus strains (M38 weakly positive, M11 negative). All hydrolised casein but the effect was more rapid with one of the B pumilus (M11) strains. It is concluded that not only do the toxins of B pumilus differ from those of B cereus, but there are also differences between the toxins produced by the two strains of B pumilus (M11 and M38).<\/jats:p>","DOI":"10.1136\/jcp.44.6.455","type":"journal-article","created":{"date-parts":[[2007,6,9]],"date-time":"2007-06-09T00:50:46Z","timestamp":1181350246000},"page":"455-458","source":"Crossref","is-referenced-by-count":27,"title":["Toxin production by Bacillus pumilus."],"prefix":"10.1136","volume":"44","author":[{"given":"B","family":"Hoult","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A F","family":"Tuxford","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[1991,6,1]]},"reference":[{"key":"ref_1","first-page":"177","article-title":"Pulmonary disease due to inhalation of derivatives of Bacillus subtilis containing proteolytic enzyme","volume":"1","author":"Flindt, M.L.H.","year":"1969","unstructured":"Flindt MLH. Pulmonary disease due to inhalation of derivatives of Bacillus subtilis containing proteolytic enzyme. Lancet 1969;ii: 1 177-81.","journal-title":"Lancet"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1128\/IAI.35.1.289-295.1982","article-title":"Bacillus pumilus in the induction of clindamycin-associated enterocolitis in guinea pigs","volume":"35","author":"Brophy, P.F.; Knoop, F.C.","year":"1982","unstructured":"Brophy PF, Knoop FC. Bacillus pumilus in the induction of clindamycin-associated enterocolitis in guinea pigs. Infect Immun 1982;35:289-95.","journal-title":"Infect Immun"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1136\/jcp.32.3.289","article-title":"Severe clinical conditions associated with Bacillus cereus and the apparent involvement of exotoxins","volume":"32","author":"Turnbull, P.C.B.; J0rgensen, K.; Kramer, J.M.","year":"1979","unstructured":"Turnbull PCB, J0rgensen K, Kramer JM, et al. 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J Med Microbiol 1987;23:179-86.","journal-title":"J Med Microbiol"},{"key":"ref_12","first-page":"328","article-title":"Quantitative untersuchengen uber die wirkung des diphtherietoxins auf das wachstum und den stoffwechel der gewebskulturen. Zentr Bakt Parasit Abt I orig 1930; 117","author":"Krontowski, A.A.","unstructured":"Krontowski AA. Quantitative untersuchengen uber die wirkung des diphtherietoxins auf das wachstum und den stoffwechel der gewebskulturen. Zentr Bakt Parasit Abt I orig 1930;117:328-48."},{"key":"ref_13","first-page":"147","article-title":"Antibodies to Bacillus species in sera of Danish cotton workers. Proceedings ofthe 13th Cotton Dust Research Conference","author":"Tuxford, A.F.; Hoult, B.; Sigsgaard, T.","year":"1989","unstructured":"Tuxford AF, Hoult B, Sigsgaard T. In: Jacobs RR, Wakelyn PJ, eds. Antibodies to Bacillus species in sera of Danish cotton workers. 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