{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T14:58:13Z","timestamp":1761058693910,"version":"3.44.0"},"reference-count":37,"publisher":"BMJ","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clin Pathol"],"published-print":{"date-parts":[[1991,6]]},"abstract":"<jats:p>A highly sensitive non-isotopic in situ hybridisation technique was developed for the localisation of Epstein-Barr virus (EBV) in paraffin wax embedded tissue sections. The method uses a repeated sequence of the EBV genome as a probe, labelled with the novel reporter molecule, digoxigenin. The method can identify individual copies of EBV by detection of both EBV DNA and highly localised RNA transcripts. A combination of careful proteolytic digestion of tissue sections, high temperature denaturation of probe and target DNA, and sensitive immunocytochemical detection are used to attain single copy sensitivity. The technique is quicker and simpler to perform than some other methods used for the identification of EBV, and provides simultaneous morphological information which cannot be obtained by methods using tissue extracts. This method permits the investigation of the role of EBV in neoplastic conditions of lymphoid and epithelial cells, and may prove valuable in determining the sites of latent virus in healthy subjects.<\/jats:p>","DOI":"10.1136\/jcp.44.6.487","type":"journal-article","created":{"date-parts":[[2007,6,9]],"date-time":"2007-06-09T00:50:46Z","timestamp":1181350246000},"page":"487-491","source":"Crossref","is-referenced-by-count":20,"title":["Detection of single copies of Epstein-Barr virus in paraffin wax sections by non-radioactive in situ hybridisation."],"prefix":"10.1136","volume":"44","author":[{"given":"P J","family":"Coates","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W P","family":"Mak","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G","family":"Slavin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A J","family":"d'Ardenne","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[1991,6,1]]},"reference":[{"key":"ref_1","first-page":"263","article-title":"Immunological responses to EpsteinBarr virus infection and the pathogenesis ofEBV-induced diseases","volume":"948","author":"Thorley-Lawson, D.A.","year":"1988","unstructured":"Thorley-Lawson DA. Immunological responses to EpsteinBarr virus infection and the pathogenesis ofEBV-induced diseases. Biochim Biophys Acta 1988;948:263-86.","journal-title":"Biochim Biophys Acta"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1073\/pnas.59.1.94","article-title":"Relation of Burkitt's tumourassociated herpes-type virus to infectious mononucleosis","volume":"59","author":"Henle, G.; Henle, W.; Diehl, V.","year":"1968","unstructured":"Henle G, Henle W, Diehl V. Relation of Burkitt's tumourassociated herpes-type virus to infectious mononucleosis. Proc Natl Acad Sci USA 1968;59:94-101.","journal-title":"Proc Natl Acad Sci USA"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1080","DOI":"10.1097\/00007890-199006000-00010","article-title":"Epstein-Barr virus-determined clonality in posttransplant lymphoproliferative disease","volume":"49","author":"Patton, D.F.; Wilkowski, C.W.; Hanson, C.A.","year":"1990","unstructured":"Patton DF, Wilkowski CW, Hanson CA, et al. Epstein-Barr virus-determined clonality in posttransplant lymphoproliferative disease. Transplantation 1990;49:1080-4.","journal-title":"Transplantation"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"792","DOI":"10.1182\/blood.V73.3.792.bloodjournal733792","article-title":"Molecular genetic analysis of three AIDS-associated neoplasms of uncertain lineage demonstrates their B-cell derivation and the possible pathogenetic role of the Epstein-Barr virus","volume":"73","author":"Knowles, G.M.; Inghirami, G.; Ubriaco, A.; Dalla-Favera, R.","year":"1989","unstructured":"Knowles GM, Inghirami G, Ubriaco A, Dalla-Favera R. Molecular genetic analysis of three AIDS-associated neoplasms of uncertain lineage demonstrates their B-cell derivation and the possible pathogenetic role of the Epstein-Barr virus. Blood 1989;73:792-9.","journal-title":"Blood"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1002\/jmv.1890260307","article-title":"Detection of EBV and HPV DNA sequences in oral \"hairy\" leukoplakia by in situ hybridization","volume":"26","author":"Eversole, L.R.; Stone, C.E.; Beckmann, A.M.","year":"1988","unstructured":"Eversole LR, Stone CE, Beckmann AM. Detection of EBV and HPV DNA sequences in oral \"hairy\" leukoplakia by in situ hybridization. J Med Virol 1988;26:271-7.","journal-title":"J Med Virol"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1002\/ijc.2910160103","article-title":"Nasopharyngeal carcinoma, X: Presence of Epstein-Barr virus genomes in separated epithelial cells of tumors in patients","volume":"16","author":"Desgranges, C.; Wolf, H.; de-The, G.","year":"1975","unstructured":"Desgranges C, Wolf H, de-The G, et al. Nasopharyngeal carcinoma, X: Presence of Epstein-Barr virus genomes in separated epithelial cells of tumors in patients from Singapore, Tunisia, and Kenya. Int J Cancer 1975;16: 7-15.","journal-title":"Int J Cancer"},{"key":"ref_7","first-page":"86","article-title":"Epstein-Barr viral DNA in tissues of Hodgkin's disease","volume":"129","author":"Weiss, L.M.; Strickler, J.G.; Warnke, R.A.; Purtilo, D.T.; Sklar, J.","year":"1987","unstructured":"Weiss LM, Strickler JG, Warnke RA, Purtilo DT, Sklar J. Epstein-Barr viral DNA in tissues of Hodgkin's disease. Am JPathol 1987;129:86-91.","journal-title":"Am JPathol"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"689","DOI":"10.1056\/NEJM198903163201103","article-title":"Hodgkin's disease and Epstein-Barr virus. Altered antibody patterns before diagnosis","volume":"320","author":"Mueller, N.; Evans, A.; Harris, N.L.","year":"1989","unstructured":"Mueller N, Evans A, Harris NL, et al. Hodgkin's disease and Epstein-Barr virus. Altered antibody patterns before diagnosis. N Engl J Med 1989;320:689-95.","journal-title":"N Engl J Med"},{"key":"ref_9","first-page":"541","article-title":"Analysis of DNA in fresh and fixed tissue by the polymerase chain reaction","volume":"136","author":"Rogers, B.B.; Alpert, L.C.; Hine, E.A.S.; Buffone, G.J.","year":"1990","unstructured":"Rogers BB, Alpert LC, Hine EAS, Buffone GJ. Analysis of DNA in fresh and fixed tissue by the polymerase chain reaction. Am J Pathol 1990;136:541-8.","journal-title":"Am J Pathol"},{"key":"ref_10","first-page":"711","article-title":"Detection of Epstein-Barr virus genomes in archival tissues by polymerase chain reaction","volume":"114","author":"Peiper, S.C.; Myers, J.L.; Broussard, E.E.; Sixbey, J.W.","year":"1990","unstructured":"Peiper SC, Myers JL, Broussard EE, Sixbey JW. Detection of Epstein-Barr virus genomes in archival tissues by polymerase chain reaction. Arch Pathol Lab Med 1990; 114:711-14.","journal-title":"Arch Pathol Lab Med"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1136\/jcp.44.2.115","article-title":"Simplified procedures for applying the polymerase chain reaction to routinely fixed paraffin wax sections","volume":"44","author":"Coates, P.J.; d'Ardenne, A.J.; Kahn, G.; Kangro, H.O.; Slavin, G.","year":"1991","unstructured":"Coates PJ, d'Ardenne AJ, Kahn G, Kangro HO, Slavin G. Simplified procedures for applying the polymerase chain reaction to routinely fixed paraffin wax sections. J Clin Pathol 1991;44:115-18.","journal-title":"J Clin Pathol"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"796","DOI":"10.1016\/0046-8177(89)90075-0","article-title":"Identification of Epstein-Barr virus-infected cells in tonsils of acute infectious mononucleosis by in situ hybridization","volume":"20","author":"Niedobitek, G.; Hamilton-Dutoit, S.; Herbst, H.","year":"1989","unstructured":"Niedobitek G, Hamilton-Dutoit S, Herbst H, et al. Identification of Epstein-Barr virus-infected cells in tonsils of acute infectious mononucleosis by in situ hybridization. Hum Pathol 1989;20:796-9.","journal-title":"Hum Pathol"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"625","DOI":"10.1097\/00000478-198908000-00001","article-title":"Analysis of lymphoepithelioma and lymphoepithelioma-like carcinomas for Epstein-Barr viral genomes by in situ hybridization","volume":"13","author":"Weiss, L.M.; Movahed, L.A.; Butler, A.E.","year":"1989","unstructured":"Weiss LM, Movahed LA, Butler AE, et al. Analysis of lymphoepithelioma and lymphoepithelioma-like carcinomas for Epstein-Barr viral genomes by in situ hybridization. Am J Surg Pathol 1989;13:625-31.","journal-title":"Am J Surg Pathol"},{"key":"ref_14","first-page":"651","article-title":"In situ demonstration of EpsteinBarr viral genomes in viral associated B cell lymphoproliferations","volume":"134","author":"Weiss, L.M.; Movahed, L.A.","year":"1989","unstructured":"Weiss LM, Movahed LA. In situ demonstration of EpsteinBarr viral genomes in viral associated B cell lymphoproliferations. Am J Pathol 1989;134:651-9.","journal-title":"Am J Pathol"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"554","DOI":"10.1016\/S0140-6736(89)90093-7","article-title":"Identification of EBV-DNA in tumour cells of AIDS-related lymphomas by in situ hybridization","volume":"i:","author":"Hamilton-Dutoit, S.J.; Pallesen, G.; Karkov, J.","year":"1989","unstructured":"Hamilton-Dutoit SJ, Pallesen G, Karkov J, et al. Identification of EBV-DNA in tumour cells of AIDS-related lymphomas by in situ hybridization. Lancet 1989;i:554-5.","journal-title":"Lancet"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"502","DOI":"10.1056\/NEJM198902233200806","article-title":"Detection of Epstein-Barr viral genomes in Reed-Stemnberg cells of Hodgkin's disease","volume":"320","author":"Weiss, L.M.; Movahed, L.A.; Warnke, R.A.; Sklar, J.","year":"1989","unstructured":"Weiss LM, Movahed LA, Warnke RA, Sklar J. Detection of Epstein-Barr viral genomes in Reed-Stemnberg cells of Hodgkin's disease. N Engl J Med 1989;320:502-6.","journal-title":"N Engl J Med"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"810","DOI":"10.1182\/blood.V74.2.810.810","article-title":"Demonstration ofmonoclonal EBV genomes in Hodgkin's disease and KI-l-positive anaplastic large cell lymphoma by combined Southern blot and in situ hybridization","volume":"74","author":"Anagnostopoulos, I.; Herbst, H.; Niedobitek, G.; Stein, H.","year":"1989","unstructured":"Anagnostopoulos I, Herbst H, Niedobitek G, Stein H. Demonstration ofmonoclonal EBV genomes in Hodgkin's disease and KI-l-positive anaplastic large cell lymphoma by combined Southern blot and in situ hybridization. Blood 1989;74:810-16.","journal-title":"Blood"},{"key":"ref_18","first-page":"89","article-title":"Detection of Epstein-Barr virus genomes by in situ hybridization with a terminally biotin-labeled synthetic oligonculeotide probe from the EBV NotI and PstI tandem repeat regions. Mod Pathol","volume":"3","author":"Montone, K.T.; Budgeon, L.R.; Brigati, D.J.","year":"1990","unstructured":"Montone KT, Budgeon LR, Brigati DJ. Detection of Epstein-Barr virus genomes by in situ hybridization with a terminally biotin-labeled synthetic oligonculeotide probe from the EBV NotI and PstI tandem repeat regions. Mod Pathol 1990;3:89-96."},{"issue":"1","key":"ref_19","first-page":"972","article-title":"Persistent active Epstein-Barr virus infection and atypical lymphoproliferation. Report of two cases","volume":"1","author":"Kreuger, G.R.F.; Papadakis, T.; Schaefer, H.J.","year":"1987","unstructured":"Kreuger GRF, Papadakis T, Schaefer HJ. Persistent active Epstein-Barr virus infection and atypical lymphoproliferation. Report of two cases. Am J Surg Pathol 1987;1 1: 972-81.","journal-title":"Am J Surg Pathol"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"733","DOI":"10.1056\/NEJM198803243181203","article-title":"T-cell lymphomas containing Epstein-Barr viral DNA in patients with chronic Epstein-Barr virus infections","volume":"318","author":"Jones, J.F.; Shurin, S.; Abramowsky, C.","year":"1988","unstructured":"Jones JF, Shurin S, Abramowsky C, et al. T-cell lymphomas containing Epstein-Barr viral DNA in patients with chronic Epstein-Barr virus infections. N Engl J Med 1988;318:733-41.","journal-title":"N Engl J Med"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"220","DOI":"10.1136\/jcp.43.3.220","article-title":"Demonstration of Epstein-Barr virus in primary brain lymphoma by in situ DNA hybridization in paraffin wax embedded tissue","volume":"43","author":"Murphy, J.K.; Young, L.S.; Bevan, I.S.","year":"1990","unstructured":"Murphy JK, Young LS, Bevan IS, et al. Demonstration of Epstein-Barr virus in primary brain lymphoma by in situ DNA hybridization in paraffin wax embedded tissue. J Clin Pathol 1990;43:220-3.","journal-title":"J Clin Pathol"},{"key":"ref_22","first-page":"8473","article-title":"Epstein-Barr virus genomes in lymphoid cells: Activation in mitosis","volume-title":"and chromosomal location. Proc Nati Acad Sci USA","volume":"84","author":"Teo, C.G.; Griffin, B.E.","year":"1987","unstructured":"Teo CG, Griffin BE. Epstein-Barr virus genomes in lymphoid cells: Activation in mitosis and chromosomal location. Proc Nati Acad Sci USA 1987;84:8473-7."},{"key":"ref_23","first-page":"359","article-title":"Persistence of Epstein-Barr virus in salivary gland biopsies from healthy individuals and patients with Sjorgens syndrome. Clin Exp Immunol","volume":"75","author":"Venables, P.J.W.; Teo, C.G.; Baboonian, C.","year":"1989","unstructured":"Venables PJW, Teo CG, Baboonian C, et al. Persistence of Epstein-Barr virus in salivary gland biopsies from healthy individuals and patients with Sjorgens syndrome. Clin Exp Immunol 1989;75:359-64."},{"key":"ref_24","first-page":"1035","article-title":"Detection of EpsteinBarr virus by in situ hybridization. Progress toward development of a nonisotopic diagnostic test","volume":"135","author":"Bashir, R.; Hochberg, F.; Singer, R.H.","year":"1989","unstructured":"Bashir R, Hochberg F, Singer RH. Detection of EpsteinBarr virus by in situ hybridization. Progress toward development of a nonisotopic diagnostic test. Am J Pathol 1989;135: 1035-44.","journal-title":"Am J Pathol"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"858","DOI":"10.1136\/jcp.40.8.858","article-title":"Detection of low copy human papilloma virus DNA and mRNA in routine paraffin sections of cervix by non-isotopic in situ hybridization","volume":"40","author":"Burns, J.; Graham, A.K.; Frank, C.","year":"1987","unstructured":"Burns J, Graham AK, Frank C, et al. Detection of low copy human papilloma virus DNA and mRNA in routine paraffin sections of cervix by non-isotopic in situ hybridization. J Clin Pathol 1987;40:858-64.","journal-title":"J Clin Pathol"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"865","DOI":"10.1136\/jcp.40.8.865","article-title":"Rapid technique ofDNA-DNA in situ hybridization on formalin fixed tissue sections using microwave irradiation","volume":"40","author":"Coates, P.J.; Hall, P.A.; Butler, M.G.; d'Ardenne, A.J.","year":"1987","unstructured":"Coates PJ, Hall PA, Butler MG, d'Ardenne AJ. Rapid technique ofDNA-DNA in situ hybridization on formalin fixed tissue sections using microwave irradiation. J Clin Pathol 1987;40:865-9.","journal-title":"J Clin Pathol"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1016\/0166-0934(89)90020-7","article-title":"situ hybridization; an optimised detection protocol for a biotinylated DNA probe renders it more sensitive than a comparable \"Slabelled probe","volume":"24","author":"Allan, G.M.","year":"1989","unstructured":"Allan GM, Todd D, Smyth JA, et al. In situ hybridization; an optimised detection protocol for a biotinylated DNA probe renders it more sensitive than a comparable \"Slabelled probe. J Virol Methods 1989;24:181-90.","journal-title":"J Virol Methods"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1136\/jcp.44.1.33","article-title":"Interphase cytogenetics using biotin and digoxigenin probes III: Increased sensitivity andflexibility ofdigoxigenin-labelled DNA probes for HPV detection in cervical biopsy specimens and cell lines","volume":"44","author":"Herrington, C.S.; Graham, A.K.; O'D, McGee J.","year":"1991","unstructured":"Herrington CS, Graham AK, McGee J O'D. Interphase cytogenetics using biotin and digoxigenin probes III: Increased sensitivity andflexibility ofdigoxigenin-labelled DNA probes for HPV detection in cervical biopsy specimens and cell lines. J Clin Pathol 1991;44:33-8.","journal-title":"J Clin Pathol"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1016\/0042-6822(83)90460-9","article-title":"Detection of viral genomes in cultured cells and paraffin-embedded tissue sections using biotin-labeled hybridization probes","volume":"126","author":"Brigati, D.J.; Myerson, D.; Leary, J.L.","year":"1983","unstructured":"Brigati DJ, Myerson D, Leary JL, et al. Detection of viral genomes in cultured cells and paraffin-embedded tissue sections using biotin-labeled hybridization probes. Virology 1983;126:32-50.","journal-title":"Virology"},{"key":"ref_30","first-page":"174","article-title":"In situ hybridization analysis of HPV 16 DNA sequences in early cervical neoplasia","volume":"123","author":"Crum, C.P.; Nagai, N.; Levine, R.U.; Silverstein, S.","year":"1986","unstructured":"Crum CP, Nagai N, Levine RU, Silverstein S. In situ hybridization analysis of HPV 16 DNA sequences in early cervical neoplasia. Am J Pathol 1986;123:174-82.","journal-title":"Am J Pathol"},{"key":"ref_31","first-page":"587","article-title":"Sensitivity of in situ detection with biotinylated probes of human papilloma virus in frozen tissue sections of squamous cell carcinomas of the cervix","volume":"131","author":"Walboomers, J.M.M.; Melchers, W.J.G.; Mullink, H.","year":"1988","unstructured":"Walboomers JMM, Melchers WJG, Mullink H, et al. Sensitivity of in situ detection with biotinylated probes of human papilloma virus in frozen tissue sections of squamous cell carcinomas of the cervix. Am J Pathol 1988; 131:587-94.","journal-title":"Am J Pathol"},{"key":"ref_32","first-page":"978","article-title":"In situ hybridization with digoxigenin-labeled DNA of human papillomavirus (HPV 16\/18) in HeLa and SiHa cells","volume":"6","author":"Heiles, H.B.J.; Genersch, E.; Kessler, C.; Neumann, R.; Eggers, H.J.","year":"1988","unstructured":"Heiles HBJ, Genersch E, Kessler C, Neumann R, Eggers HJ. In situ hybridization with digoxigenin-labeled DNA of human papillomavirus (HPV 16\/18) in HeLa and SiHa cells. Biotechniques 1988;6:978-81.","journal-title":"Biotechniques"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"800","DOI":"10.1136\/jcp.43.10.800","article-title":"Sensitivity of digoxigenin and biotin labelled probes for detection ofhuman papillomavirus by in situ hybridisation","volume":"43","author":"Morris, R.G.; Arends, M.J.; Bishop, P.E.; Sizer, K.; Duvall, E.; Bird, C.C.","year":"1990","unstructured":"Morris RG, Arends MJ, Bishop PE, Sizer K, Duvall E, Bird CC. Sensitivity of digoxigenin and biotin labelled probes for detection ofhuman papillomavirus by in situ hybridisation. J Clin Pathol 1990;43:800-5.","journal-title":"J Clin Pathol"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"806","DOI":"10.1136\/jcp.43.10.806","article-title":"In situ hybridization with digoxigenin-labelled DNA probes for detection of viral genomes","volume":"43","author":"Furuta, Y.; Shinohara, T.; Sano, K.; Meguro, M.; Nagashima, K.","year":"1990","unstructured":"Furuta Y, Shinohara T, Sano K, Meguro M, Nagashima K. In situ hybridization with digoxigenin-labelled DNA probes for detection of viral genomes. J Clin Pathol 1990; 43:806-9.","journal-title":"J Clin Pathol"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"286","DOI":"10.1128\/JVI.44.1.286-294.1982","article-title":"The long internal direct repeat in Epstein-Barr virus DNA","volume":"44","author":"Cheung, A.; Kieff, E.","year":"1982","unstructured":"Cheung A, Kieff E. The long internal direct repeat in Epstein-Barr virus DNA. J Virol 1982;44:286-94.","journal-title":"J Virol"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1016\/0092-8674(89)90924-0","article-title":"Highly localized distribution ofspecific transcripts within interphase nuclei visualized by in situ hybridization","volume":"57","author":"Lawrence, J.B.; Singer, R.H.; Marselle, R.M.","year":"1989","unstructured":"Lawrence JB, Singer RH, Marselle RM. Highly localized distribution ofspecific transcripts within interphase nuclei visualized by in situ hybridization. Cell 1989;57:493-502.","journal-title":"Cell"},{"key":"ref_37","first-page":"381","article-title":"In situ hybridization: a new tool in pathology","volume":"45","author":"Warford, A.","year":"1988","unstructured":"Warford A. In situ hybridization: a new tool in pathology. Med Lab Sci 1988;45:381-94.","journal-title":"Med Lab Sci"}],"container-title":["Journal of Clinical Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1136\/jcp.44.6.487","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,9,25]],"date-time":"2025-09-25T12:18:02Z","timestamp":1758802682000},"score":1,"resource":{"primary":{"URL":"https:\/\/jcp.bmj.com\/lookup\/doi\/10.1136\/jcp.44.6.487"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1991,6]]},"references-count":37,"journal-issue":{"issue":"6","published-online":{"date-parts":[[1991,6,1]]},"published-print":{"date-parts":[[1991,6]]}},"alternative-id":["10.1136\/jcp.44.6.487"],"URL":"https:\/\/doi.org\/10.1136\/jcp.44.6.487","relation":{},"ISSN":["0021-9746","1472-4146"],"issn-type":[{"type":"print","value":"0021-9746"},{"type":"electronic","value":"1472-4146"}],"subject":[],"published":{"date-parts":[[1991,6]]}}}