{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T05:14:20Z","timestamp":1790399660101,"version":"4.1.0"},"reference-count":22,"publisher":"BMJ","issue":"7","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clin Pathol"],"published-print":{"date-parts":[[1991,7]]},"abstract":"<jats:p>Whether phagocytosis of Staphylococcus aureus by polymorphonuclear neutrophils, monocytes, and peritoneal macrophages takes place when the staphylococci are adherent to monolayers of human mesothelial cells in the absence of opsonins was investigated. Adherence of S aureus to mesothelial monolayers increased significantly when the bacteria were opsonised with pooled human serum, but phagocytosis by polymorphonuclear neutrophils and monocytes occurred independently. Phagocytosis by peritoneal macrophages, however, was only marginal. Pretreatment of polymorphonuclear neutrophils with inhibitors of cellular metabolism and motility reduced their phagocytic capacity. These results indicate that the surface of mesothelial cells provides favourable conditions for the elimination of staphylococci in the peritoneal cavity. Phagocytic motility seems to be important in surface phagocytosis. The inability of peritoneal macrophages to phagocytise staphylococci adherent to the mesothelial cells suggests that they contribute little to the antibacterial defence of the peritoneal membrane of patients receiving peritoneal dialysis.<\/jats:p>","DOI":"10.1136\/jcp.44.7.600","type":"journal-article","created":{"date-parts":[[2007,6,9]],"date-time":"2007-06-09T00:50:46Z","timestamp":1181350246000},"page":"600-604","source":"Crossref","is-referenced-by-count":19,"title":["Role of mesothelial cells in peritoneal antibacterial defence."],"prefix":"10.1136","volume":"44","author":[{"given":"M A","family":"Muijsken","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H J","family":"Heezius","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J","family":"Verhoef","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H A","family":"Verbrugh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"239","published-online":{"date-parts":[[1991,7,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1177\/089686088300303S02","article-title":"Peritonitis related death among CAPD patients","volume":"3","author":"Fenton, S.A.A.","year":"1983","unstructured":"Fenton SAA. 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Scand J Clin Lab Invest 1968;97: S77-S89.","journal-title":"Scand J Clin Lab Invest"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"539","DOI":"10.1136\/jcp.31.6.539","article-title":"Phagocytosis and killing of staphylococci by human polymorphonuclear and mononuclear leukocytes","volume":"31","author":"Verbrugh, H.A.; Peters, R.; Peterson, P.K.; Verhoef, J.","year":"1978","unstructured":"Verbrugh HA, Peters R, Peterson PK, Verhoef J. Phagocytosis and killing of staphylococci by human polymorphonuclear and mononuclear leukocytes. 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J Infect Dis 1990;161:266-73.","journal-title":"J Infect Dis"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"250","DOI":"10.1128\/IAI.50.1.250-254.1985","article-title":"Phagocytosis of staphylococci by human polymorphonuclear leukocytes is enhanced in the presence of endothelial cells","volume":"50","author":"Vandenbroucke-Grauls, C.M.J.E.; Thijssen, H.M.W.M.; Verhoef, J.","year":"1985","unstructured":"Vandenbroucke-Grauls CMJE, Thijssen HMWM, Verhoef J. Phagocytosis of staphylococci by human polymorphonuclear leukocytes is enhanced in the presence of endothelial cells. Infect Immun 1985;50:250-4.","journal-title":"Infect Immun"},{"key":"ref_20","first-page":"163","article-title":"Phagocytosis","volume-title":"The neutrophil. Function and clinical disorders","author":"Klebanoff, S.J.; Clark, R.A.","year":"1978","unstructured":"Klebanoff SJ, Clark RA. Phagocytosis. In: Klebanoff SJ, Clark RA, eds. The neutrophil. Function and clinical disorders. 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