{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T06:29:52Z","timestamp":1770704992806,"version":"3.49.0"},"reference-count":31,"publisher":"BMJ","issue":"8","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clin Pathol"],"published-print":{"date-parts":[[1991,8]]},"abstract":"<jats:p>Using a newly established HTLV-1 positive T cell line as an immunogen, a new monoclonal antibody, Ber-ACT8, was produced. It reacts with in vitro activated T cells and a small subset of normal resting T cells, but not with resting B cells or any of the 29 established human permanent cell lines tested. Immunohistological analysis of a wide spectrum of human tissues showed that Ber-ACT8 reactivity is restricted to a few T cells in the peripheral blood, the extrafollicular areas of lymph nodes and tonsils, and splenic red pulp. In the gut Ber-ACT8 labelled most intraepithelial T cells and up to 50% of lamina propria T cells. The antibody also immunostained T cells present in the oral and bronchial mucosa. Double labelling on splenic cells, fresh blood lymphocytes, and in vitro activated T cells showed that most Ber-ACT8 positive cells coexpressed CD8. Ber-ACT8 did not react with any of the 14 Hodgkin's lymphomas nor any of the 172 non-Hodgkin's lymphomas tested, with the exception of 10 cases of T cell lymphomas, five of which were located in the jejunum and associated with coeliac disease, and one B cell lymphoma, and most cases of hairy cell leukaemia tested. Parallel immunostainings with Ber-ACT8, anti-TCR-beta (beta F1), and anti-TCR-delta showed that most Ber-ACT8 positive T cells carry the TCR of alpha beta type. Comparison of Ber-ACT8 with HML-1, B-ly7, and LF61 showed essentially the same reactivity and an identical molecular target. The molecular structure recognised seems to be a trimeric molecule with components of 150, 125 and 105 kilodaltons, with the Ber-ACT8 epitope localised on the 150 kilodalton chain. The 150 kilodalton molecule contains an 0-linked carbohydrate moiety of about 10 kilodaltons. Because of its very selective distribution, the trimeric antigen is a powerful reagent for the diagnosis of gut T cell-derived T cell lymphomas and other extranodal T cell lymphomas, as well as hairy cell leukaemia.<\/jats:p>","DOI":"10.1136\/jcp.44.8.636","type":"journal-article","created":{"date-parts":[[2007,6,9]],"date-time":"2007-06-09T04:50:46Z","timestamp":1181364646000},"page":"636-645","source":"Crossref","is-referenced-by-count":35,"title":["Ber-ACT8: new monoclonal antibody to the mucosa lymphocyte antigen."],"prefix":"10.1136","volume":"44","author":[{"given":"M","family":"Kruschwitz","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G","family":"Fritzsche","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R","family":"Schwarting","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K","family":"Micklem","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D Y","family":"Mason","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B","family":"Falini","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H","family":"Stein","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[1991,8,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1398","DOI":"10.4049\/jimmunol.126.4.1398","article-title":"A monoclonal antibody (anti-Tac) reactive with activated and functionally mature human T cells. Expression of Tac antigen on activated cytotoxic killer T cells, suppressor cells, and on one of two types of helper T cells","volume":"126","author":"Uchiyama, T.; Nelson, D.L.; Fleisher, T.A.; Waldmann, T.A.","year":"1981","unstructured":"Uchiyama T, Nelson DL, Fleisher TA, Waldmann TA. A monoclonal antibody (anti-Tac) reactive with activated and functionally mature human T cells. Expression of Tac antigen on activated cytotoxic killer T cells, suppressor cells, and on one of two types of helper T cells. J Immunol 1981;126:1398-403.","journal-title":"J Immunol"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1038\/299065a0","article-title":"Production of a monoclonal antibody specific for Hodgkin and StembergReed cells of Hodgkin's disease and a subset of normal lymphoid cells","volume":"299","author":"Schwab, U.; Stein, H.; Gerdes, J.","year":"1982","unstructured":"Schwab U, Stein H, Gerdes J, et al. Production of a monoclonal antibody specific for Hodgkin and StembergReed cells of Hodgkin's disease and a subset of normal lymphoid cells. Nature 1982;299:65-7.","journal-title":"Nature"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"445","DOI":"10.1002\/ijc.2910300411","article-title":"Identification ofHodgkin and Steinberg-Reed cells as a unique cell type derived from a newly-detected small cell population","volume":"30","author":"Stein, H.; Gerdes, J.; Schwab, U.","year":"1982","unstructured":"Stein H, Gerdes J, Schwab U, et al. Identification ofHodgkin and Steinberg-Reed cells as a unique cell type derived from a newly-detected small cell population. Int J Cancer 1982;30:445-59.","journal-title":"Int J Cancer"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"162","DOI":"10.1002\/eji.1830110220","article-title":"Surface antigens specifically expressed by activated T cells in humans","volume":"11","author":"Corte, G.; Moretta, L.; Damiani, G.; Mingari, M.C.; Bargellesi, A.","year":"1981","unstructured":"Corte G, Moretta L, Damiani G, Mingari MC, Bargellesi A. Surface antigens specifically expressed by activated T cells in humans. Eur J Immunol 1981;11:162-5.","journal-title":"Eur J Immunol"},{"key":"ref_5","article-title":"typing III","author":"Stein, H.; Gerdes, J.; Schwarting, R.; Froese, P.; Lemke, H.","unstructured":"Stein H, Gerdes J, Schwarting R, Froese P, Lemke H. In: McMichael AJ, ed. Leukocyte typing III. Oxford: Oxford University Press, 1987:574."},{"key":"ref_6","article-title":"Leucocyte typing IV: White cell differentiation antigens","author":"Knapp, W.; Dorken, B.; Rieber, E.P.","year":"1990","unstructured":"Knapp W, Dorken B, Rieber EP, et al. Leucocyte typing IV: White cell differentiation antigens. Oxford: Oxford University Press, 1990."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"320","DOI":"10.1182\/blood.V75.1.320.320","article-title":"Specificities of monoclonal antibodies B-ly7 and HML-1 are identical","volume":"75","author":"Schwarting, R.; Dienemann, D.; Kruschwitz, M.; Fritzsche, G.; Stein, H.","year":"1990","unstructured":"Schwarting R, Dienemann D, Kruschwitz M, Fritzsche G, Stein H. Specificities of monoclonal antibodies B-ly7 and HML-1 are identical. Blood 1990;75:320.","journal-title":"Blood"},{"key":"ref_8","first-page":"292","article-title":"Updated Kiel classification for lymphomas","volume":"i:","author":"Stansfield, A.G.; Diebold, J.; Kapanci, Y.","year":"1988","unstructured":"Stansfield AG, Diebold J, Kapanci Y, et al. Updated Kiel classification for lymphomas. Lancet 1988;i:292-3.","journal-title":"Lancet"},{"key":"ref_9","first-page":"121","article-title":"Phenotypic and genotypic markers in malignant lymphomas: Cellular origin of Hodgkin and Steinberg-Reed cells and implications for the classification of T-cell and B-cell lymphomas","volume-title":"Pathology of cell receptors and tumor markers. Application of immunocytochemistry and hybridization in tumor diagnosis","author":"Stein, H.; Gerdes, J.; Falini, B.","year":"1987","unstructured":"Stein H, Gerdes J, Falini B. Phenotypic and genotypic markers in malignant lymphomas: Cellular origin of Hodgkin and Steinberg-Reed cells and implications for the classification of T-cell and B-cell lymphomas. In: Seifert G, Hubner K, eds. Pathology of cell receptors and tumor markers. Application of immunocytochemistry and hybridization in tumor diagnosis. Stuttgart: Gustav Fischer Verlag, 1987:121-44."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"2281","DOI":"10.4049\/jimmunol.134.4.2281","article-title":"Coexpression of T4 and T8 on peripheral blood T cells demonstrated by two-color fluorescence flow cytometry","volume":"134","author":"Blue, M.L.; Daley, J.F.; Levine, H.; Schlossman, S.F.","year":"1985","unstructured":"Blue ML, Daley JF, Levine H, Schlossman SF. Coexpression of T4 and T8 on peripheral blood T cells demonstrated by two-color fluorescence flow cytometry. J Immunol 1985;134:2281-6.","journal-title":"J Immunol"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"2844","DOI":"10.1073\/pnas.75.6.2844","article-title":"\"Panning\" for lymphocytes: A method for cell selection","volume":"75","author":"Wysocki, L.J.; Sato, V.L.","year":"1978","unstructured":"Wysocki LJ, Sato VL. \"Panning\" for lymphocytes: A method for cell selection. Proc Natl Acad Sci USA 1978;75:2844-8.","journal-title":"Proc Natl Acad Sci USA"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1002\/ijc.2910310104","article-title":"Production of a monoclonal antibody reactive with a human nuclear antigen associated with cell proliferation","volume":"31","author":"Gerdes, J.; Schwab, U.; Lemke, H.; Stein, H.","year":"1983","unstructured":"Gerdes J, Schwab U, Lemke H, Stein H. Production of a monoclonal antibody reactive with a human nuclear antigen associated with cell proliferation. Int J Cancer 1983;31:13-20.","journal-title":"Int J Cancer"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1177\/32.2.6198355","article-title":"Immunoenzymatic labeling of monoclonal antibodies using immune complexes of alkaline phosphatase and monoclonal antialkaline phosphatase (APAAP complexes)","volume":"32","author":"Cordell, J.; Falini, B.; Erber, O.N.","year":"1984","unstructured":"Cordell J, Falini B, Erber ON, et al. Immunoenzymatic labeling of monoclonal antibodies using immune complexes of alkaline phosphatase and monoclonal antialkaline phosphatase (APAAP complexes). J Histochem Cytochem 1984;32:219-29.","journal-title":"J Histochem Cytochem"},{"key":"ref_14","first-page":"676","article-title":"Use of freezedried paraffin-embedded sections for immunohistologic staining with monoclonal antibodies","volume":"52","author":"Stein, H.; Gatter, K.; Asbahr, H.; Mason, D.Y.","year":"1985","unstructured":"Stein H, Gatter K, Asbahr H, Mason DY. Use of freezedried paraffin-embedded sections for immunohistologic staining with monoclonal antibodies. Lab Invest 1985; 52:676-83.","journal-title":"Lab Invest"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"426","DOI":"10.1016\/S0076-6879(84)08109-X","article-title":"Biosynthetics, surface labeling, and isolation of membrane immunoglobulin","volume":"108","author":"Yuan, D.; Vitetta, E.S.","year":"1984","unstructured":"Yuan D, Vitetta ES. Biosynthetics, surface labeling, and isolation of membrane immunoglobulin. Methods Enzymol 1984;108:426-33.","journal-title":"Methods Enzymol"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"3005","DOI":"10.4049\/jimmunol.132.6.3005","article-title":"Characterization of human interleukin 2 receptor (Tac-antigen) in normal and leukemic T cells: Co-expression of normal and aberrant receptors on HUT-102 cells","volume":"132","author":"Wano, Y.; Uchiyama, T.; Fukui, K.; Maeda, M.; Uchino, H.; Yodoi, J.","year":"1984","unstructured":"Wano Y, Uchiyama T, Fukui K, Maeda M, Uchino H, Yodoi J. Characterization of human interleukin 2 receptor (Tac-antigen) in normal and leukemic T cells: Co-expression of normal and aberrant receptors on HUT-102 cells. J Immunol 1984;132:3005.","journal-title":"J Immunol"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1279","DOI":"10.1002\/eji.1830170910","article-title":"A monoclonal antibody (HML-1) defining a novel membrane molecule present on human intestinal lymphocytes","volume":"17","author":"Cerf-Bensussan, N.; Jarry, A.; Brousse, N.; Lisowska-Grospierre, B.; Guy-Grand, D.; Griscelli, C.","year":"1987","unstructured":"Cerf-Bensussan N, Jarry A, Brousse N, Lisowska-Grospierre B, Guy-Grand D, Griscelli C. A monoclonal antibody (HML-1) defining a novel membrane molecule present on human intestinal lymphocytes. 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J Immunol 1990;145:3247-55.","journal-title":"J Immunol"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"502","DOI":"10.1002\/eji.1830150515","article-title":"VLA- 1: a T cell surface antigen which defines a novel late stage of human T cell activation","volume":"15","author":"Hemler, M.; Jacobson, J.G.; Brenner, M.B.; Mann, D.; Strominger, J.L.","year":"1985","unstructured":"Hemler M, Jacobson JG, Brenner MB, Mann D, Strominger JL. VLA-1: a T cell surface antigen which defines a novel late stage of human T cell activation. Eur J Immunol 1985;15:502-8.","journal-title":"Eur J Immunol"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"855","DOI":"10.1038\/333855a0","article-title":"Expression of the y-3 T-cell receptor on intestinal CD8 intraepithelial lymphocytes","volume":"333","author":"Goodman, T.; Lefrancois, L.","year":"1988","unstructured":"Goodman T, Lefrancois L. Expression of the y-3 T-cell receptor on intestinal CD8 intraepithelial lymphocytes. Nature 1988;333:855-8.","journal-title":"Nature"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"688","DOI":"10.1016\/S0140-6736(85)92930-7","article-title":"Malignant histiocytosis of the intestine: a T cell lymphoma","volume":"ii:","author":"Isaacson, P.G.; O'Connor, N.T.J.; Spencer, J.","year":"1985","unstructured":"Isaacson PG, O'Connor NTJ, Spencer J, et al. Malignant histiocytosis of the intestine: a T cell lymphoma. Lancet 1985;ii:688-91.","journal-title":"Lancet"},{"key":"ref_24","first-page":"1","article-title":"Enteropathyassociated T cell lymphoma (malignant histiocytosis ofthe intestine) is recognized by monoclonal antibody (HML-1) that defines a membrane molecule on human mucosal lymphocytes","volume":"132","author":"Spencer, J.; Cerf-Bensussan, N.; Jarry, A.","year":"1988","unstructured":"Spencer J, Cerf-Bensussan N, Jarry A, et al. Enteropathyassociated T cell lymphoma (malignant histiocytosis ofthe intestine) is recognized by monoclonal antibody (HML-1) that defines a membrane molecule on human mucosal lymphocytes. Am JPathol 1988;132:1-5.","journal-title":"Am JPathol"},{"key":"ref_25","first-page":"1053","article-title":"Identification ofa T cell lymphoma category derived from intestinal mucosa-associated T cells","volume":"ii:","author":"Stein, H.; Dienemann, D.; Sperling, M.; Zeitz, M.; Riecken, E.O.","year":"1988","unstructured":"Stein H, Dienemann D, Sperling M, Zeitz M, Riecken EO. Identification ofa T cell lymphoma category derived from intestinal mucosa-associated T cells. 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Lancet 1989; i:223.","journal-title":"Lancet"},{"key":"ref_28","first-page":"107","article-title":"Monoclonal antibody (HML-1) reactivity of T-cell lymphomas","volume":"1","author":"Brousse, N.; Jarry, A.; Peuchmaur, M.","year":"1989","unstructured":"Brousse N, Jarry A, Peuchmaur M, et al. Monoclonal antibody (HML-1) reactivity of T-cell lymphomas. Lancet 1989;ii:1 107-8.","journal-title":"Lancet"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"959","DOI":"10.1182\/blood.V76.5.959.959","article-title":"B-ly-7, a monoclonal antibody reactive with hairy cell leukemia, also defines an activation antigen on normal CD8+ T cells","volume":"76","author":"Mulligan, S.P.; Travade, P.; Matutes, E.","year":"1990","unstructured":"Mulligan SP, Travade P, Matutes E, et al. B-ly-7, a monoclonal antibody reactive with hairy cell leukemia, also defines an activation antigen on normal CD8+ T cells. Blood 1990;76:959-64.","journal-title":"Blood"},{"key":"ref_30","first-page":"955","article-title":"Reactivity of T cells in mycosis fungoides exhibiting marked epidermotropism with the monoclonal antibody HML-1 that defines a membrane molecule on human mucosal lymphocytes","volume":"134","year":"1989","unstructured":"Sperling M, Kaudewitz P, Braun-Falco 0, Stein H. Reactivity of T cells in mycosis fungoides exhibiting marked epidermotropism with the monoclonal antibody HML-1 that defines a membrane molecule on human mucosal lymphocytes. Am J Pathol 1989;134:955-60.","journal-title":"Am J Pathol"},{"key":"ref_31","first-page":"1053","article-title":"A unique phenotype of skin-associated lymphocytes in humans","volume":"136","author":"Picker, L.J.; Michie, S.A.; Rott, L.S.; Butcher, E.C.","year":"1990","unstructured":"Picker LJ, Michie SA, Rott LS, Butcher EC. A unique phenotype of skin-associated lymphocytes in humans. 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