{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,21]],"date-time":"2025-11-21T17:36:32Z","timestamp":1763746592717,"version":"3.45.0"},"reference-count":22,"publisher":"BMJ","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clin Pathol"],"published-print":{"date-parts":[[1992,1]]},"abstract":"<jats:p>AIMS: To review the performance of the Venereal Diseases Research Laboratory (VDRL) test and the Treponema pallidum haemagglutination assay (TPHA) as a combined screen for syphilis to provide a baseline for assessing screening by anti-treponemal IGG EIA. METHODS: Between 1980 and 1987 all serum samples were screened by both VDRL and TPHA tests. The FTA-ABS test was also used in suspected early primary syphilis, or when one of the other tests was positive. A positive result in a screening test was confirmed by quantitative testing. From 1988 all specimens were screened with an enzyme immunoassay (Captia Syph G) as a single screening test. RESULTS: Of the 44 primary, 47 secondary, and 38 early latent cases of syphilis, the VDRL and TPHA detected 32 (73%) and 31 (71%) of the primary cases; the combination detected 37 (84%). All 85 cases of cases of secondary and early latent infection were reactive in the TPHA test, whereas the VDRL was reactive in only 68 (80%). EIA had a reported sensitivity of 82% for primary infection. CONCLUSIONS: EIA can be used as a single screening test for detecting early syphilis because its results are comparable with those of the combined VDRL and TPHA tests. The conventional VDRL test should not be used as a single screening test.<\/jats:p>","DOI":"10.1136\/jcp.45.1.37","type":"journal-article","created":{"date-parts":[[2007,6,9]],"date-time":"2007-06-09T00:50:46Z","timestamp":1181350246000},"page":"37-41","source":"Crossref","is-referenced-by-count":33,"title":["Enzyme immunoassay for anti-treponemal IgG: screening or confirmatory test?"],"prefix":"10.1136","volume":"45","author":[{"given":"H","family":"Young","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A","family":"Moyes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A","family":"McMillan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J","family":"Patterson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[1992,1,1]]},"reference":[{"key":"ref_1","first-page":"588","article-title":"Syphilis, yaws and pinta","volume-title":"Wilson's principles of bacteriology, virology and immunology","author":"Young, H.; Penn, C.W.","year":"1990","unstructured":"Young H, Penn CW. Syphilis, yaws and pinta. In: Smith GR, Easmon CSF, eds. Topley and Wilson's principles of bacteriology, virology and immunology. Edward Arnold, 1990:588-604."},{"key":"ref_2","first-page":"249","article-title":"Serological diagnosis of syphilis: current methods","volume-title":"Immunological diagnosis of sexually transmitted diseases","author":"Luger, A.F.H.","year":"1988","unstructured":"Luger AFH. Serological diagnosis of syphilis: current methods. In: Young H, McMillan A, eds. Immunological diagnosis of sexually transmitted diseases. New York: Marcel Dekker, 1988:249-74."},{"key":"ref_3","first-page":"72","article-title":"Screening for treponemal infection by a new enzyme immunoassay","volume":"65","author":"Young, H.; Moyes, A.; McMillan, A.; Robertson, D.H.","year":"1989","unstructured":"Young H, Moyes A, McMillan A, Robertson DH. Screening for treponemal infection by a new enzyme immunoassay. Genitourin Med 1989;65:72-8.","journal-title":"Genitourin Med"},{"key":"ref_4","first-page":"341","article-title":"Treponema pallidum haemagglutination test as a screening procedure for the diagnosis of syphilis","volume":"5","author":"Young, H.; Henrichsen, C.; Robertson, D.H.H.","year":"1974","unstructured":"Young H, Henrichsen C, Robertson DHH. Treponema pallidum haemagglutination test as a screening procedure for the diagnosis of syphilis. Br J Vener Dis 1974;5O: 341-6.","journal-title":"Br J Vener Dis"},{"key":"ref_5","first-page":"117","article-title":"Service Communicable Disease Surveillance Centre. Sexually transmitted disease in Britain: 1985-6","volume":"65","author":"Public Health Laboratory","year":"1989","unstructured":"Public Health Laboratory Service Communicable Disease Surveillance Centre. Sexually transmitted disease in Britain: 1985-6. Genitourin Med 1989;65:117-21.","journal-title":"Genitourin Med"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"166","DOI":"10.1016\/S0140-6736(89)90232-8","article-title":"Resurgence of heterosexually acquired early syphilis in London letter","volume":"ii:","author":"Tang, A.; Barlow, D.","year":"1989","unstructured":"Tang A, Barlow D. Resurgence of heterosexually acquired early syphilis in London letter. Lancet 1989;ii:166-7.","journal-title":"Lancet"},{"key":"ref_7","first-page":"53","article-title":"Progress toward achieving the 1990 objectives for the Nation for sexually transmitted diseases","volume":"39","year":"1990","unstructured":"Progress toward achieving the 1990 objectives for the Nation for sexually transmitted diseases. Morbidity and Mortality Weekly Report 1990;39:53-7.","journal-title":"Morbidity and Mortality Weekly Report"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"409","DOI":"10.1136\/bmj.299.6696.409","article-title":"Antenatal screening for syphilis","volume":"229","author":"Clay, J.C.","year":"1989","unstructured":"Clay JC. Antenatal screening for syphilis. Br Med J 1989; 229:409-10.","journal-title":"Br Med J"},{"key":"ref_9","first-page":"368","article-title":"Is antenatal screening for syphilis worthwhile?","volume":"151","author":"Garland, S.M.; Kelly, V.N.","year":"1989","unstructured":"Garland SM, Kelly VN. Is antenatal screening for syphilis worthwhile? Med J Aust 1989;151:368-70.","journal-title":"Med J Aust"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"162","DOI":"10.5694\/j.1326-5377.1988.tb112802.x","article-title":"Congenital syphilis-should we worry?","volume":"148","author":"Gilbert, G.L.","year":"1988","unstructured":"Gilbert GL. Congenital syphilis-should we worry? Med J Aust 1988;148:162-4.","journal-title":"Med J Aust"},{"key":"ref_11","first-page":"113","volume":"179","author":"Syphilis, Rufli T.; Infection, H.I.V.","year":"1989","unstructured":"Rufli T. Syphilis and HIV Infection. Dermatologica 1989; 179:113-7.","journal-title":"Dermatologica"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"492","DOI":"10.7326\/0003-4819-107-4-492","article-title":"Seronegative secondary syphilis in a patient infected with the human immunodeficiency virus (HIV) with Kaposi sarcoma","volume":"107","author":"Hicks, C.B.; Benson, P.M.; Lupton, G.P.; Tramont, E.C.","year":"1987","unstructured":"Hicks CB, Benson PM, Lupton GP, Tramont EC. Seronegative secondary syphilis in a patient infected with the human immunodeficiency virus (HIV) with Kaposi sarcoma. Ann Intern Med 1987;107:492-5.","journal-title":"Ann Intern Med"},{"key":"ref_13","first-page":"239","article-title":"Primary and secondary syphilis, 20 years' experience. 3: diagnosis, treatment, and follow up","volume":"65","author":"Anderson, J.; Mindel, A.; Tovey, S.J.; Williams, P.","year":"1989","unstructured":"Anderson J, Mindel A, Tovey SJ, Williams P. Primary and secondary syphilis, 20 years' experience. 3: diagnosis, treatment, and follow up. Gemtourin Med 1989;65: 239-43.","journal-title":"Gemtourin Med"},{"key":"ref_14","first-page":"227","article-title":"Application of the enzyme-linked immunosorbent assay (ELISA) in the serodiagnosis of syphilis","volume":"51","author":"Veldkamp, J.; Visser, A.M.","year":"1975","unstructured":"Veldkamp J, Visser AM. Application of the enzyme-linked immunosorbent assay (ELISA) in the serodiagnosis of syphilis. Br J Vener Dis 1975;51:227-31.","journal-title":"Br J Vener Dis"},{"key":"ref_15","first-page":"231","article-title":"Evaluation of a solid-phase enzyme immunoassay in the diagnosis of syphilis","volume":"2","author":"Borobio, M.V.; Alvarez-Dardet, C.; Gallardo, R.M.","year":"1985","unstructured":"Borobio MV, Alvarez-Dardet C, Gallardo RM. Evaluation of a solid-phase enzyme immunoassay in the diagnosis of syphilis. Eur J Sex Transm Dis 1985;2:231-3.","journal-title":"Eur J Sex Transm Dis"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1128\/JCM.21.3.399-402.1985","article-title":"Evaluation of an enzyme-linked immunosorbent assay for treponemal antibody","volume":"21","author":"Stevens, R.W.; Schmitt, M.E.","year":"1985","unstructured":"Stevens RW, Schmitt ME. Evaluation of an enzyme-linked immunosorbent assay for treponemal antibody. 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J Clin Microbiol 1984;20:1109-13.","journal-title":"J Clin Microbiol"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"264","DOI":"10.1093\/infdis\/151.2.264","article-title":"Antigens of Treponema pallidum recognized by IgG and IgM antibodies during syphilis in humans","volume":"151","author":"Baker-Zander, S.A.; Hook, E.W.; Bonin, P.; Handsfield, H.H.; Lukehart, S.A.","year":"1985","unstructured":"Baker-Zander SA, Hook EW, Bonin P, Handsfield HH, Lukehart SA. Antigens of Treponema pallidum recognized by IgG and IgM antibodies during syphilis in humans. J Infect Dis 1985;151:264-72.","journal-title":"J Infect Dis"},{"key":"ref_19","first-page":"87","article-title":"Syphilis with a negative blood test reaction","volume":"189","author":"Spangler, A.S.; Jackson, J.H.; Fiumara, N.J.; Warthin, T.A.","year":"1964","unstructured":"Spangler AS, Jackson JH, Fiumara NJ, Warthin TA. Syphilis with a negative blood test reaction. JAMA 1964; 189:87-90.","journal-title":"JAMA"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1001\/archderm.125.1.15","article-title":"Centers for Disease Control, Atlanta. Recommendations for diagnosing and treating syphilis in HIV-infected patients","volume":"125","author":"Morbidity; Report, Mortality","year":"1989","unstructured":"Morbidity and Mortality Report. Centers for Disease Control, Atlanta. Recommendations for diagnosing and treating syphilis in HIV-infected patients. Arch Dermatol 1989;125:15-6.","journal-title":"Arch Dermatol"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"541","DOI":"10.1212\/WNL.40.3_Part_1.541","article-title":"Neurosyphilis in ATDS patients: initial CSF VDRL may be negative","volume":"40","author":"Feraru, E.R.; Aronow, H.A.; Lipton, R.B.","year":"1990","unstructured":"Feraru ER, Aronow HA, Lipton RB. Neurosyphilis in ATDS patients: initial CSF VDRL may be negative. Neurology 1990;40:541-3.","journal-title":"Neurology"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"975","DOI":"10.1016\/0002-9378(90)91107-N","article-title":"False-negative syphilis screening: the prozone phenomenon, non-immune hydrops, and diagnosis ofsyphilis during pregnancy","volume":"163","author":"Berkowitz, K.; Boxi, L.; Fox, H.E.","year":"1990","unstructured":"Berkowitz K, Boxi L, Fox HE. False-negative syphilis screening: the prozone phenomenon, non-immune hydrops, and diagnosis ofsyphilis during pregnancy. 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