{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,14]],"date-time":"2025-12-14T16:43:03Z","timestamp":1765730583496,"version":"3.48.0"},"reference-count":10,"publisher":"BMJ","issue":"10","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clin Pathol"],"published-print":{"date-parts":[[1992,10]]},"abstract":"<jats:p>AIMS: To ascertain the present practices of Scottish microbiological laboratories for examining faecal samples for Cryptosporidium spp and to compare these with practices elsewhere. METHOD: A questionnaire was circulated to all 35 Scottish NHS bacteriological laboratories, where human stool samples are subjected to routine microbiological examination, to determine current laboratory testing methods and selection criteria used to examine faecal specimens for Cryptosporidium spp. RESULTS: All laboratories responded, of which the first began testing in 1984 and the last in 1990. At 15 sites all diagnostic stool samples submitted are routinely examined for Cryptosporidium spp. Elsewhere, selection criteria, principally based on age, specific request, and foreign travel, are used. The age distribution of Scottish cases differed significantly from that of the PHLS study of 16 laboratories (p &lt; 0.001), probably as a result of the selection criteria used. The phenol-auramine staining procedure is used by 19 laboratories, with modified Ziehl-Neelsen staining used by 14 laboratories; eight used a second, confirmatory method. CONCLUSIONS: Currently applied selection criteria for examination of stools for Cryptosporidium in Scotland do not accord with published epidemiological data. Specimen selection based on age of patient and stool consistency may lead to cases of public health importance being missed. Nationally agreed criteria for testing of human samples based on epidemiological evidence are required.<\/jats:p>","DOI":"10.1136\/jcp.45.10.914","type":"journal-article","created":{"date-parts":[[2007,6,9]],"date-time":"2007-06-09T00:50:46Z","timestamp":1181350246000},"page":"914-916","source":"Crossref","is-referenced-by-count":0,"title":["Laboratory examination for Cryptosporidium spp in Scotland."],"prefix":"10.1136","volume":"45","author":[{"given":"D M","family":"Campbell","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P A","family":"Flanagan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[1992,10,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"592","DOI":"10.1016\/S0016-5085(76)80503-3","article-title":"Acute enterocolitis in a human being infected with the protozoan Cryptosporidium","volume":"70","author":"Nime, F.A.; Burek, J.D.; Page, D.L.","year":"1976","unstructured":"Nime FA, Burek JD, Page DL, et al. 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Annual Reports 1984-1990"},{"key":"ref_5","first-page":"774","article-title":"Cryptosporidiosis in England and Wales: prevalence and epidemiological features","volume":"300","author":"Palmer, SR, Biffin; A.H.","year":"1990","unstructured":"Palmer SR, Biffin AH. Cryptosporidiosis in England and Wales: prevalence and epidemiological features. Br MedJ7 1990;300:774-7."},{"issue":"445-5","key":"ref_6","first-page":"1","article-title":"Laboratory methods for diagnosing cryptosporidiosis","volume":"44","author":"Casemore, D.P.","year":"1991","unstructured":"Casemore DP. Laboratory methods for diagnosing cryptosporidiosis. J Clin Pathol 1991;44:445-5 1.","journal-title":"J Clin Pathol"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"458","DOI":"10.1128\/MMBR.50.4.458-483.1986","article-title":"Cryptosporidium spp. and cryptosporidiosis","volume":"50","author":"Fayer, R.; Ungar, L.P.","year":"1986","unstructured":"Fayer R, Ungar LP. Cryptosporidium spp. and cryptosporidiosis. 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Detection of Cryptosporidium oocysts in human fecal specimens by an individual immunofluorescence assay with monoclonal antibodies. J Clin Microbiol 1989;27:1 135-6.","journal-title":"J Clin Microbiol; Detection of Cryptosporidium oocysts in human fecal specimens by an individual immunofluorescence assay with monoclonal antibodies"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1490","DOI":"10.1128\/JCM.27.7.1490-1495.1989","article-title":"Comparison of conventional staining methods and monoclonal antibody-based methods for Cryptosporidium oocyst detection","volume":"27","author":"Arrowood, M.J.; Sterling, C.R.","year":"1989","unstructured":"Arrowood MJ, Sterling CR. Comparison of conventional staining methods and monoclonal antibody-based methods for Cryptosporidium oocyst detection. 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