{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T22:05:13Z","timestamp":1791324313250,"version":"4.3.1"},"reference-count":19,"publisher":"BMJ","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clin Pathol"],"published-print":{"date-parts":[[1992,6]]},"abstract":"<jats:p>AIMS: To determine a possible mechanism to explain the presence of aluminium lines within fully calcified bone in aluminium-related osteomalacia. METHODS: Fifty five bone cases shown by bone biopsy to be aluminium-related osteomalacia were studied. In 38 specimens aluminium lines were identified within calcified bone by means of the Aluminon stain and a characteristic form of patchy mineralisation was seen within thickened osteoid seams. Five representative examples were analysed quantitatively by histomorphometry and electronprobe X-ray microanalysis and compared with five cases of vitamin D deficiency-related osteomalacia which also had patchy mineralisation. RESULTS: The patchy calcification occupied 40 +\/- 8% (mean +\/- SEM) of the osteoid and consisted of small focal deposits (less than 40 microns diameter), often (52%) around osteoid osteocytes (probably an underestimate of the association), and larger areas that extended to the aluminium lines at the underlying mineralisation front. Small and large mineralisation nuclei were seen ultrastructurally in the patchy calcification. Quantitative electronprobe X-ray microanalysis showed that calcium concentrations and calcium:phosphorus ratios in the mineralisation nuclei and in the superficial layer of the fully calcified bone of the aluminium-related osteomalacia cases were significantly less than values measured at similar sites in the vitamin D deficiency-related osteomalacia cases. Furthermore, aluminium could not be detected by means of this technique at the mineralisation front or along cement lines in these specimens. CONCLUSIONS: Calcification can occur in thickened osteoid seams in osteomalacia. It can begin around osteoid osteocytes as small deposits that enlarge within the osteoid and extend to the underlying mineralisation front or cement line where aluminium lines may become trapped. Complete calcification of osteoid could account for the presence of aluminium lines within fully calcified bone. The Aluminon stain appears to be a more sensitive method for the detection of aluminium in bone than electronprobe X-ray microanalysis.<\/jats:p>","DOI":"10.1136\/jcp.45.6.502","type":"journal-article","created":{"date-parts":[[2007,6,9]],"date-time":"2007-06-09T00:50:46Z","timestamp":1181350246000},"page":"502-508","source":"Crossref","is-referenced-by-count":40,"title":["Histological and electron microprobe studies of mineralisation in aluminium-related osteomalacia."],"prefix":"10.1136","volume":"45","author":[{"given":"B F","family":"Boyce","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J","family":"Byars","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S","family":"McWilliams","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M Z","family":"Mocan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H Y","family":"Elder","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I T","family":"Boyle","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B J","family":"Junor","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"239","published-online":{"date-parts":[[1992,6,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1038\/ki.1981.149","article-title":"Aluminum localization in bone from hemodialyzed patients: relationship to matrix mineralization","volume":"20","author":"Coumot-Witmer, G.; ZingraffJ, Plachot; J.J.","year":"1981","unstructured":"Coumot-Witmer G, ZingraffJ, Plachot JJ, et al. Aluminum localization in bone from hemodialyzed patients: relationship to matrix mineralization. Kidney Int 1981;20:375-85.","journal-title":"Kidney Int"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1009","DOI":"10.1016\/S0140-6736(82)90049-6","article-title":"Hypercalcaemic osteomalacia due to aluminium toxicity","volume":"ii:","author":"Boyce, B.F.; Fell, G.S.; Elder, H.Y.","year":"1982","unstructured":"Boyce BF, Fell GS, Elder HY, et al. Hypercalcaemic osteomalacia due to aluminium toxicity. Lancet 1982; ii: 1009-13.","journal-title":"Lancet"},{"key":"ref_3","first-page":"709","article-title":"The prevalence of bone aluminum deposition in renal osteodystrophy and its relation to the response to calcitriol therapy. NEngl JMed","volume":"307","author":"Ott, S.M.; Maloney, N.A.; Coburn, J.W.; Alfrey, A.C.; Sherrard, D.J.","year":"1982","unstructured":"Ott SM, Maloney NA, Coburn JW, Alfrey AC, Sherrard DJ. The prevalence of bone aluminum deposition in renal osteodystrophy and its relation to the response to calcitriol therapy. NEngl JMed 1982;307:709-13."},{"key":"ref_4","first-page":"206","article-title":"Histologic quantitation of aluminum in iliac bone from patients with renal failure","volume":"99","author":"Maloney, N.A.; Ott, S.M.; Alfrey, A.; Miller, N.L.; Coburn, J.W.; Sherrard, D.J.","year":"1982","unstructured":"Maloney NA, Ott SM, Alfrey A, Miller NL, Coburn JW, Sherrard DJ. Histologic quantitation of aluminum in iliac bone from patients with renal failure. J Lab Clin Med 1982;99:206-16.","journal-title":"J Lab Clin Med"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"657","DOI":"10.1136\/bmj.2.6088.657","article-title":"Composition of the domestic water supply and the incidence of fractures and encephalopathy in patients on home dialysis","volume":"ii:","author":"Platts, M.M.; Goode, G.C.; Hislop, J.S.","year":"1977","unstructured":"Platts MM, Goode GC, Hislop JS. Composition of the domestic water supply and the incidence of fractures and encephalopathy in patients on home dialysis. Br Med J 1977;ii:657-60.","journal-title":"Br Med J"},{"key":"ref_6","first-page":"101","article-title":"Aluminium toxicity during regular haemodialysis","author":"Elliot, H.L.; Dryburgh, F.; Fell, G.S.; Sabat, S.; MacDougall, A.I.","year":"1978","unstructured":"Elliot HL, Dryburgh F, Fell GS, Sabat S, MacDougall AI. Aluminium toxicity during regular haemodialysis. Br Med J 1978;i:l101-3.","journal-title":"Br Med J"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1159\/000166631","article-title":"Osteomalacia in chronic renal failure: A syndrome previously reported only with maintenance dialysis","volume":"2","author":"Felsenfeld, A.J.; Gutman, R.A.; Llach, F.; Harrelson, J.M.","year":"1982","unstructured":"Felsenfeld AJ, Gutman RA, Llach F, Harrelson JM. Osteomalacia in chronic renal failure: A syndrome previously reported only with maintenance dialysis. Am J Nephrol 1982;2:147-54.","journal-title":"Am J Nephrol"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1079","DOI":"10.1056\/NEJM198404263101704","article-title":"Aluminum intoxication from aluminum-containing phosphate binders in children with azotemia not undergoing hemodialysis","volume":"310","author":"Andreoli, S.P.; Bergstein, J.M.; Sherrard, D.J.","year":"1984","unstructured":"Andreoli SP, Bergstein JM, Sherrard DJ. Aluminum intoxication from aluminum-containing phosphate binders in children with azotemia not undergoing hemodialysis. N Engl J Med 1984;310:1079-84.","journal-title":"N Engl J Med"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1352","DOI":"10.1136\/jcp.34.12.1352","article-title":"Haemodialysisrelated osteomalacia: a staining method to demonstrate aluminium","volume":"34","author":"Buchanan, M.R.C.; Ihle, B.U.; Dunn, C.M.","year":"1981","unstructured":"Buchanan MRC, Ihle BU, Dunn CM. Haemodialysisrelated osteomalacia: a staining method to demonstrate aluminium J Clin Pathol 1981;34:1352-4.","journal-title":"J Clin Pathol"},{"key":"ref_10","article-title":"X-ray microanalysis in the electron microscope","author":"Chandler, J.A.","year":"1977","unstructured":"Chandler JA. X-ray microanalysis in the electron microscope. Amsterdam: North Holland, 1977."},{"key":"ref_11","first-page":"157","article-title":"The microprobe assay of chemical elements. Optical techniques","volume-title":"techniques in biological research","author":"Hall, H.A.","year":"1975","unstructured":"Hall HA. The microprobe assay of chemical elements. Optical techniques. In: Oster G, ed. Physical techniques in biological research. 2nd Edition. New York: Academic Press, 1975:157-275.","edition":"2nd Edition"},{"key":"ref_12","first-page":"25","article-title":"Optimising thin film X-ray spectra for quantitative analysis","volume":"125","author":"Nicholson, W.A.P.; Gray, C.C.; Chapman, J.N.; Robertson, B.W.","year":"1982","unstructured":"Nicholson WAP, Gray CC, Chapman JN, Robertson BW. Optimising thin film X-ray spectra for quantitative analysis. JMicrosc 1982;125:25-40.","journal-title":"JMicrosc"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1016\/8756-3282(86)90250-4","article-title":"High bone turnover associated with an aluminium-induced impairment of bone formation","volume":"7","author":"Charhon, S.A.; Chavassieux, P.M.; Chapuy, M.C.; Traeger, J.; Meunier, P.J.","year":"1986","unstructured":"Charhon SA, Chavassieux PM, Chapuy MC, Traeger J, Meunier PJ. High bone turnover associated with an aluminium-induced impairment of bone formation. Bone 1986;7:319-24.","journal-title":"Bone"},{"key":"ref_14","first-page":"821","article-title":"Focal osteomalacia due to low-dose diphosphonate therapy in Paget's disease","volume":"i:","author":"Boyce, B.F.; Smith, L.; Fogelman, I.; Johnston, E.; Ralston, S.; Boyle, I.T.","year":"1984","unstructured":"Boyce BF, Smith L, Fogelman I, Johnston E, Ralston S, Boyle IT. Focal osteomalacia due to low-dose diphosphonate therapy in Paget's disease. Lancet 1984;i:821-4.","journal-title":"Lancet"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1002\/jbmr.5650020103","article-title":"The effect of Iahydroxyvitamin D3 on the mineralization defect in disodium etidronate-treated Paget's disease-a doubleblind randomized clinical study","volume":"2","author":"Ralston, S.H.; Boyce, B.F.; Cowan, R.A.","year":"1987","unstructured":"Ralston SH, Boyce BF, Cowan RA, et al. The effect of Iahydroxyvitamin D3 on the mineralization defect in disodium etidronate-treated Paget's disease-a doubleblind randomized clinical study. J Bone Min Res 1987;2: 5-11.","journal-title":"J Bone Min Res"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1007\/978-94-009-1868-9_11","article-title":"Cellular and subcellular localization of aluminum: Histochemistry","volume-title":"renal failure","author":"Freemont, A.J.","year":"1990","unstructured":"Freemont AJ. Cellular and subcellular localization of aluminum: Histochemistry. In: De Broe ME, Coburn JW, eds. Aluminum and renal failure. The Netherlands: Kluwer, 1990:155-66."},{"key":"ref_17","first-page":"18","article-title":"Changes in bone histology after treatment with deferoxamine","volume":"29","author":"Ott, S.M.; Andress, D.C.; Nebeker, H.G.","year":"1986","unstructured":"Ott SM, Andress DC, Nebeker HG, et al. Changes in bone histology after treatment with deferoxamine. Kidney Int 1986;29,S18:108-13.","journal-title":"Kidney Int"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1042\/cs0730227","article-title":"Deferoxamineinduced bone changes in haemodialysis patients-a histomorphometric study","volume":"73","author":"Charhon, S.A.; Chavassieux, P.; Boivin, G.","year":"1987","unstructured":"Charhon SA, Chavassieux P, Boivin G, et al. Deferoxamineinduced bone changes in haemodialysis patients-a histomorphometric study. Clin Sci 1987;73:227-34.","journal-title":"Clin Sci"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1136\/jcp.37.2.136","article-title":"Detection and distribution of aluminium in bone","volume":"37","author":"Denton, J.; Freemont, A.J.; Ball, J.","year":"1984","unstructured":"Denton J, Freemont AJ, Ball J. Detection and distribution of aluminium in bone. 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