{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T09:11:11Z","timestamp":1784193071891,"version":"3.55.0"},"reference-count":25,"publisher":"BMJ","issue":"10","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clin Pathol"],"published-print":{"date-parts":[[1993,10]]},"abstract":"<jats:p>AIMS--To study the value of non-isotopic in situ hybridisation (NISH) in detecting human papillomavirus (HPV) infection in female genital lesions positive for the virus by conventional histology but negative by filter DNA hybridisation. METHODS--Forty three cases, which showed the histological hallmarks of the HPV infection but produced negative results in filter dot blot hybridisation tests (Vira Pap and Vira Type kits), were identified in the course of an investigation of 304 vaginal, vulvar, and cervical samples from 267 patients. These cases were studied by NISH for the presence of HPV infection. RESULTS--In 28 (65%) of the cases NISH gave a positive hybridisation signal. In 26 cases (96%) the signal was diffuse, and in two (4%) punctate or diffuse, representing episomal and episomal or integrated HPV DNA, respectively. In most cases only a few HPV positive cells were discernible. CONCLUSION--NISH is a more sensitive technique than dot blot hybridisation, detecting HPV infection in most cases which show histological HPV atypia but which remain negative in filter DNA hybridisation. Thus NISH is useful as an additional technique to verify the presence of HPV in lesions which remain negative in filter hybridisation tests.<\/jats:p>","DOI":"10.1136\/jcp.46.10.936","type":"journal-article","created":{"date-parts":[[2007,6,9]],"date-time":"2007-06-09T00:50:46Z","timestamp":1181350246000},"page":"936-939","source":"Crossref","is-referenced-by-count":11,"title":["Detection of human papillomavirus infection by non-isotopic in situ hybridisation in condylomatous and CIN lesions."],"prefix":"10.1136","volume":"46","author":[{"given":"R","family":"P\u00f6ll\u00e4nen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S","family":"Vuopala","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V P","family":"Lehto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"239","published-online":{"date-parts":[[1993,10,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1016\/S0065-230X(08)60691-0","article-title":"Human papillomaviruses and genital cancer","volume":"48","author":"Pfister, H.","year":"1987","unstructured":"Pfister H. 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Progressive potential of mild cervical atypia: prospective cytologic, colposcopic and virological study. Lancet 1986;ii:237-40. NISH ofHPV lesions of cervix","journal-title":"Lancet"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"400","DOI":"10.1136\/jcp.44.5.400","article-title":"In situ HPV genotyping of cervical intraepithelial neoplasia in South African and UK patients: evidence for putative HPV integration in vivo","volume":"44","author":"Cooper, K.; Herrington, C.S.; Graham, A.K.; Evans, M.F.; J'OD, McGee","year":"1991","unstructured":"Cooper K, Herrington CS, Graham AK, Evans MF, McGee J'OD. In situ HPV genotyping of cervical intraepithelial neoplasia in South African and UK patients: evidence for putative HPV integration in vivo. Y Clin Pathol 1991;44:400-5.","journal-title":"Y Clin Pathol"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1007\/978-1-4757-1942-0_7","article-title":"Cervical intraepithelial neoplasia and condyloma","volume-title":"Blaustein's pathology of the female genital tract","author":"Ferenczy, A.; Winkler, B.","year":"1987","unstructured":"Ferenczy A, Winkler B. Cervical intraepithelial neoplasia and condyloma. In: Kurman RJ, ed. Blaustein's pathology of the female genital tract. 3rd edn. New York: Springer Verlag 1987; 177-217.","edition":"3rd edn"},{"key":"ref_8","first-page":"1151","article-title":"A new type of papillomavirus DNA, its presence in genital cancer biopsies and in cell lines derived from cervical cancer","volume-title":"EMBO Y","volume":"3","author":"Boshart, M.; Gismann, L.; Ikenberg, H.; Kleinheinz, A.; Scheurlen, W.; zur Hausen, H.","year":"1984","unstructured":"Boshart M, Gismann L, Ikenberg H, Kleinheinz A, Scheurlen W, zur Hausen H. A new type of papillomavirus DNA, its presence in genital cancer biopsies and in cell lines derived from cervical cancer. EMBO Y 1984;3:1151-7."},{"key":"ref_9","first-page":"671","article-title":"Oncogenic association of specific human papillomavirus types with cervical neoplasia","volume":"79","author":"Lorinez, A.T.; Temple, G.F.; Kurman, R.J.; Jenson, A.B.; Lancaster, W.D.","year":"1987","unstructured":"Lorinez AT, Temple GF, Kurman RJ, Jenson AB, Lancaster WD. Oncogenic association of specific human papillomavirus types with cervical neoplasia. NCI 1987;79:671-7.","journal-title":"NCI"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1007\/BF02134287","article-title":"Human papillomavirus (HPV) DNA sequences demonstrated by in situ DNA hybridization in serial paraffin-embedded cervical biopsies","volume":"239","author":"Syrjianen, S.M.; Syrianen, K.J.; MAntyiArvi","year":"1986","unstructured":"Syrjianen SM, Syrianen KJ, MAntyiArvi. Human papillomavirus (HPV) DNA sequences demonstrated by in situ DNA hybridization in serial paraffin-embedded cervical biopsies. Arch Gynecol 1986;239:39-48.","journal-title":"Arch Gynecol"},{"key":"ref_11","first-page":"265","article-title":"Detection and localization of human papillomavirus DNA in human genital condylomas by in situ hybridization with biotinylated probes","volume":"16","author":"Becknmann, A.M.; Myerson, D.; Daling, JR, Kiviat; NB, Fenoglio; CM, McDougall; J.K.","year":"1985","unstructured":"Becknmann AM, Myerson D, Daling JR, Kiviat NB, Fenoglio CM, McDougall JK. Detection and localization of human papillomavirus DNA in human genital condylomas by in situ hybridization with biotinylated probes. YMed Virol 1985;16:265-73."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1097\/00004347-198509000-00005","article-title":"Specific identification of human papillomavirus type in cervical smears and paraffin sections by in situ hybridization with radioactive probes: A preliminary communication","volume":"4","author":"Gupta, J.; Gendelman, H.E.; Naghashfar, Z.","year":"1985","unstructured":"Gupta J, Gendelman HE, Naghashfar Z, et al. Specific identification of human papillomavirus type in cervical smears and paraffin sections by in situ hybridization with radioactive probes: A preliminary communication. IntJf Gynecol Pathol 1985;4:211-8.","journal-title":"IntJf Gynecol Pathol"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"505","DOI":"10.1093\/ajcp\/89.4.505","article-title":"Detection of infection by human papillomavirus in genital condylomata. A comparison study using immunocytochemistry and in situ nucleic acid hybridization","volume":"89","author":"Wilbur, D.C.; Reichman, R.C.; Stolier, M.H.","year":"1988","unstructured":"Wilbur DC, Reichman RC, Stolier MH. Detection of infection by human papillomavirus in genital condylomata. A comparison study using immunocytochemistry and in situ nucleic acid hybridization. Am Clin Pathol 1988;89:505-10.","journal-title":"Am Clin Pathol"},{"key":"ref_14","first-page":"53","article-title":"Detection of human papillomavirus DNA in oncogenital condylomata in men using in situ DNA hybridization applied to paraffin sections","volume":"21","author":"Syrjanen, S.M.; von Krogh, G.; SyriAnen, K.J.","year":"1987","unstructured":"Syrjanen SM, von Krogh G, SyriAnen KJ. Detection of human papillomavirus DNA in oncogenital condylomata in men using in situ DNA hybridization applied to paraffin sections. 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Sensitivity and specificity of various morphological features of cervical condylomas. An in situ hybridization study. Arch Pathol Lab Med 1990;114: 1038-41.","journal-title":"Arch Pathol Lab Med"},{"key":"ref_17","first-page":"283","article-title":"The demonstration of human papillomavirus 16 genomes in the nuclei of genital cancer using two different methods of in situ hybridization","volume":"65","author":"Grussendorf-Conen, E.I.; Cremer, S.","year":"1990","unstructured":"Grussendorf-Conen EI, Cremer S. The demonstration of human papillomavirus 16 genomes in the nuclei of genital cancer using two different methods of in situ hybridization. Cancer 1990;65:283-91.","journal-title":"Cancer"},{"key":"ref_18","first-page":"315","article-title":"In situ hybridization with nonisotopic probes using different detection systems","volume":"5","author":"Holm, R.; Karlsen, F.; Nesland, J.M.","year":"1992","unstructured":"Holm R, Karlsen F, Nesland JM. In situ hybridization with nonisotopic probes using different detection systems. Modem Pathol 1992;5:315-9.","journal-title":"Modem Pathol"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1016\/S0344-0338(89)80097-4","article-title":"In situ hybridization using biotinylated probes. An evaluation of different detection systems","volume":"184","author":"Niedobitek, G.; Finn, T.; Herbst, H.; Stein, H.","year":"1989","unstructured":"Niedobitek G, Finn T, Herbst H, Stein H. In situ hybridization using biotinylated probes. An evaluation of different detection systems. Pathol Res Pract 1989; 184:343-8.","journal-title":"Pathol Res Pract"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1250","DOI":"10.1016\/S0046-8177(86)80569-X","article-title":"In situ hybridization detection of human papillomavirus DNAs and messenger RNAs in genital condylomas and a cervical carcinoma","volume":"17","author":"Stoler, M.-H.; Broker, T.H.","year":"1986","unstructured":"Stoler M-H, Broker TH. In situ hybridization detection of human papillomavirus DNAs and messenger RNAs in genital condylomas and a cervical carcinoma. Hum Pathol 1986;17:1250-8.","journal-title":"Hum Pathol"},{"key":"ref_21","first-page":"349","article-title":"Integration of HPV DNA sequences in mild cervical dysplasia","volume":"5","author":"Shimoda, K.; Lancaster, W.D.","year":"1987","unstructured":"Shimoda K, Lancaster WD. Integration of HPV DNA sequences in mild cervical dysplasia. Cancer Cell 1987; 5:349-57.","journal-title":"Cancer Cell"},{"key":"ref_22","first-page":"673","article-title":"A comparison of slot blot. Southern blot and in situ hybridization analyses for human papillomavirus DNA in genital tract lesions","volume":"74","author":"Nuovo, G.J.; Richart, R.M.","year":"1989","unstructured":"Nuovo GJ, Richart RM. A comparison of slot blot. Southern blot and in situ hybridization analyses for human papillomavirus DNA in genital tract lesions. Obstet Gynecol 1989;74:673-8.","journal-title":"Obstet Gynecol"},{"key":"ref_23","first-page":"587","article-title":"DNA carcinoma inofftrhoezencertviisxs.ueAmsecJtioPnasthoolf","volume":"131","year":"1988","unstructured":"DNA carcinoma inofftrhoezencertviisxs.ueAmsecJtioPnasthoolf 1988;131:587-94."},{"key":"ref_24","first-page":"703","article-title":"Human papillomavirus infection in women with and without abnormal cervical cytology","volume":"26","author":"DeVilliers, E.M.; Schneider, A.; Miklow, H.","year":"1987","unstructured":"DeVilliers EM, Schneider A, Miklow H, et al. Human papillomavirus infection in women with and without abnormal cervical cytology. Lancet 1987;26:703-5.","journal-title":"Lancet"},{"key":"ref_25","first-page":"223","article-title":"Human papillomavirus DNA in situ hybridization may be used for the quality control of genital tract biopsies","volume":"75","author":"Richart, R.M.; Nuovo, G.J.","year":"1990","unstructured":"Richart RM, Nuovo GJ. 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