{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,7]],"date-time":"2026-02-07T22:06:16Z","timestamp":1770501976692,"version":"3.49.0"},"reference-count":19,"publisher":"BMJ","issue":"12","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clin Pathol"],"published-print":{"date-parts":[[1993,12]]},"abstract":"<jats:p>AIMS--To develop a fluorimetric method for the estimation of urinary aldosterone; to establish a normal range in 24 hour and overnight urine samples; and to investigate the use of overnight urines for detecting hyperaldosteronism. METHODS--Essential steps include hydrolysis of the 18 conjugate to release aldosterone and its oxidation with Benedict's solution, followed by thin-layer chromatography on silica gel and development of fluorescence on the plate with sulphuric acid. RESULTS--There was a linear correlation between the amount of aldosterone and the area under the peak on the chromatogram. The mean intra-assay and interassay coefficients of variation were 4.4% and 6.8%, respectively. The mean aldosterone excretion in 67 adults was 15.7 (SD 8.1) nmol\/24 hours. The mean overnight excretion in 65 adults was 2.6 (1.4) nmol\/8 hours. The method detected raised concentrations in patients with primary and secondary aldosteronism. CONCLUSIONS--This technique provides an accurate means of assaying urinary aldosterone. Overnight estimations seem to be as effective as 24 hour assays for identifying patients with hyperaldosteronism.<\/jats:p>","DOI":"10.1136\/jcp.46.12.1109","type":"journal-article","created":{"date-parts":[[2007,6,9]],"date-time":"2007-06-09T00:50:46Z","timestamp":1181350246000},"page":"1109-1112","source":"Crossref","is-referenced-by-count":4,"title":["Estimation of urinary aldosterone using thin-layer chromatography and fluorimetry."],"prefix":"10.1136","volume":"46","author":[{"given":"D","family":"Mattingly","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H","family":"Martin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C M","family":"Tyler","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[1993,12,1]]},"reference":[{"key":"ref_1","article-title":"DID 1974 RIA 1976 F 1979 RIA 1981 RIA","volume-title":"GC","year":"1981","unstructured":"DID 1974 RIA 1976 F 1979 RIA 1981 RIA 1981 RIA 1983 RIA 1986 GC"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"641","DOI":"10.1016\/0002-9343(83)91022-7","article-title":"The changing clinical spectrum of primary aldosteronism","volume":"74","author":"Bravo, E.L.; Tarazi, R.C.; Dustan, H.P.","year":"1983","unstructured":"Bravo EL, Tarazi RC, Dustan HP, et al. 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