{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,6]],"date-time":"2025-12-06T09:03:33Z","timestamp":1765011813574,"version":"3.46.0"},"reference-count":10,"publisher":"BMJ","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clin Pathol"],"published-print":{"date-parts":[[1993,4]]},"abstract":"<jats:p>AIMS--To develop an assay which would determine the parentage of hydatidiform molar pregnancies. METHODS--DNA was extracted from formalin fixed, paraffin wax embedded tissue from hydatidiform molar pregnancies and spontaneous abortions after separation of chorionic villi and decidua. PCR amplification of dinucleotide repeat sequences (\"microsatellites\") was performed using three different primers. Products were radioactively labelled and visualised by autoradiography of dried polyacrylamide gels. RESULTS--With informative microsatellites, diagnostic patterns of amplification were obtained. Complete moles yielded either one or two microsatellites which differed from both maternal (decidual) microsatellites. Complete mole could be excluded by all the microsatellites showing alleles identical with those in maternal DNA. CONCLUSIONS--This technique offers a method of determining the presence of entirely paternal alleles in a molar pregnancy and thus confirming a complete hydatidiform mole.<\/jats:p>","DOI":"10.1136\/jcp.46.4.346","type":"journal-article","created":{"date-parts":[[2007,6,9]],"date-time":"2007-06-09T00:50:46Z","timestamp":1181350246000},"page":"346-348","source":"Crossref","is-referenced-by-count":19,"title":["Diagnosis of complete molar pregnancy by microsatellites in archival material."],"prefix":"10.1136","volume":"46","author":[{"given":"S A","family":"Lane","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G R","family":"Taylor","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B","family":"Ozols","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P","family":"Quirke","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[1993,4,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1016\/S0306-3356(21)00602-6","article-title":"The pathology of trophoblastic disease: current status","volume":"2","author":"Elston, C.W.","year":"1984","unstructured":"Elston CW. The pathology of trophoblastic disease: current status. Clin Obstet Gynaecol 1984;2:135-52.","journal-title":"Clin Obstet Gynaecol"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"633","DOI":"10.1038\/268633a0","article-title":"Androgenetic origin of hydatidiform mole","volume":"268","author":"Kajii, T.; Ohama, K.","year":"1977","unstructured":"Kajii T, Ohama K. Androgenetic origin of hydatidiform mole. Nature 1977;268:633-4.","journal-title":"Nature"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1016\/0165-4608(82)90096-6","article-title":"Genetic studies on hydatidiform moles. I. The origin of partial moles","volume":"5","author":"Lawler, S.D.; Fisher, R.A.; Pickthall, V.J.; Povey, S.; Wyn Evans, M.","year":"1982","unstructured":"Lawler SD, Fisher RA, Pickthall VJ, Povey S, Wyn Evans M. Genetic studies on hydatidiform moles. I. The origin of partial moles. 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Am J Hum Genet 1989;44:388-96.","journal-title":"Am J Hum Genet"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"5804","DOI":"10.1093\/nar\/19.20.5804","article-title":"CA repeat polymorphism at the DS 582 locus proximal to adenomatous polyposis coli (APC)","volume":"19","author":"Breukel, C.; Tops, C.; van Leeuwen, C.","year":"1991","unstructured":"Breukel C, Tops C, van Leeuwen C, et al. CA repeat polymorphism at the DS 582 locus proximal to adenomatous polyposis coli (APC). Nucleic Acids Res 1991;19: 5804.","journal-title":"Nucleic Acids Res"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"216","DOI":"10.1007\/BF00204186","article-title":"DNA diagnosis of hydatidiform mole using the polymerase chain reaction","volume":"87","author":"Fukuyama, R.; Takata, M.; Kudoh, J.; Sakai, K.; Tamura, S.; Shimizu, N.","year":"1991","unstructured":"Fukuyama R, Takata M, Kudoh J, Sakai K, Tamura S, Shimizu N. DNA diagnosis of hydatidiform mole using the polymerase chain reaction. Hum Genet 1991;87: 216-18.","journal-title":"Hum Genet"}],"container-title":["Journal of Clinical Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1136\/jcp.46.4.346","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,6]],"date-time":"2025-12-06T08:59:00Z","timestamp":1765011540000},"score":1,"resource":{"primary":{"URL":"https:\/\/jcp.bmj.com\/lookup\/doi\/10.1136\/jcp.46.4.346"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1993,4]]},"references-count":10,"journal-issue":{"issue":"4","published-online":{"date-parts":[[1993,4,1]]},"published-print":{"date-parts":[[1993,4]]}},"alternative-id":["10.1136\/jcp.46.4.346"],"URL":"https:\/\/doi.org\/10.1136\/jcp.46.4.346","relation":{},"ISSN":["0021-9746","1472-4146"],"issn-type":[{"type":"print","value":"0021-9746"},{"type":"electronic","value":"1472-4146"}],"subject":[],"published":{"date-parts":[[1993,4]]}}}