{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,14]],"date-time":"2026-05-14T20:38:02Z","timestamp":1778791082027,"version":"3.51.4"},"reference-count":26,"publisher":"BMJ","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clin Pathol"],"published-print":{"date-parts":[[1993,5]]},"abstract":"<jats:p>AIM--To analyse patterns of gene expression for peptide regulatory factors in patients with Dupuytren's contracture. METHODS--Tissue samples (palmer fascia) from 12 patients with Dupuytren's contracture and 12 controls were studied using the reverse transcription\/polymerase chain reaction (RT\/PCR) technique. RESULTS--Tissue from patients with Dupuytren's contracture expressed a higher percentage of peptide regulatory factors than that of controls: interleukin-1 alpha (83% v 16%; p &lt; 0.01); interleukin-1 beta (66% v 8%; p &lt; 0.01); transforming growth factor beta (75% v 25%; p &lt; 0.02); and basic fibroblast growth factor (66% v 25%; p &lt; 0.05). Platelet derived growth factors alpha and beta were also expressed more commonly (66% v 33% and 25% v 16%, respectively), but these differences were not significant. CONCLUSIONS--The increased prevalence of expression for the above mRNAs in Dupuytren's tissue is relevant as interleukin-1, basic fibroblast growth factor, and transforming growth factor beta stimulate the growth of fibroblasts and transforming growth factor beta also enhances production of collagen and other extracellular matrix proteins. Excessive local release of these peptide regulatory factors may have an important role in the pathogenesis of Dupuytren's contracture.<\/jats:p>","DOI":"10.1136\/jcp.46.5.425","type":"journal-article","created":{"date-parts":[[2007,6,9]],"date-time":"2007-06-09T00:50:46Z","timestamp":1181350246000},"page":"425-428","source":"Crossref","is-referenced-by-count":99,"title":["Abnormal growth factor and cytokine expression in Dupuytren's contracture."],"prefix":"10.1136","volume":"46","author":[{"given":"K S","family":"Baird","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J F","family":"Crossan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S H","family":"Ralston","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[1993,5,1]]},"reference":[{"key":"ref_1","article-title":"Dupuytren's disease: biology and treatment","author":"McFarlane, R.M.; McGrouther, D.A.; Flint, M.H.","year":"1990","unstructured":"McFarlane RM, McGrouther DA, Flint MH. Dupuytren's disease: biology and treatment. Edinburgh: Churchill Livingstone, 1990."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"787","DOI":"10.2106\/00004623-198163050-00014","article-title":"Biochemical changes in collagen of the palnar fascia in patients with Dupuytren's disease","volume":"63","author":"Brickley-Parsons, D.; Glimcher, M.J.; Smith, R.J.; Albin, R.; Adams, J.","year":"1981","unstructured":"Brickley-Parsons D, Glimcher MJ, Smith RJ, Albin R, Adams J. Biochemical changes in collagen of the palnar fascia in patients with Dupuytren's disease. J Bone J'oint Surg 1981;63A:787-97.","journal-title":"J Bone J'oint Surg"},{"key":"ref_3","first-page":"7","article-title":"The distribution of unsulphated glycosaminoglycans in palmar fascia from patients with Dupuytren's disease and healthy subjects","volume":"26","author":"Tunn, S.; Gurr, E.; Delbruck, A.; Buhr, T.; Flory, J.","year":"1988","unstructured":"Tunn S, Gurr E, Delbruck A, Buhr T, Flory J. The distribution of unsulphated glycosaminoglycans in palmar fascia from patients with Dupuytren's disease and healthy subjects. J Clin Chem 1988;26:7-14.","journal-title":"J Clin Chem"},{"key":"ref_4","first-page":"1373","article-title":"Free radicals and Dupuytren's contracture","volume":"295","author":"Murrell, G.A.C.; Francis, M.J.O.; Bromley, L.","year":"1987","unstructured":"Murrell GAC, Francis MJO, Bromley L. Free radicals and Dupuytren's contracture. BrMedy 1987;295:1373-5.","journal-title":"BrMedy"},{"key":"ref_5","first-page":"709","article-title":"The genetic factor in Dupuytren's disease","volume-title":"I Bone Joint Surg","volume":"45","author":"Ling, R.S.M.","year":"1963","unstructured":"Ling RSM. The genetic factor in Dupuytren's disease. I Bone Joint Surg 1963;45-B:709-18."},{"issue":"1","key":"ref_6","first-page":"47","article-title":"The relationship of Dupuytren's contracture and epilepsy","volume":"1969","author":"James, J.I.P.","unstructured":"James JIP. The relationship of Dupuytren's contracture and epilepsy. Hand 1969;1:47-9.","journal-title":"Hand"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"729","DOI":"10.1016\/0092-8674(87)90331-X","article-title":"An oncogene isolated by transfection of Kaposi's sarcoma DNA encodes a growth factor that is a member of the FGF family","volume":"50","author":"Delli-Bovi, P.D.; Curatola, A.M.; Kern, F.G.","year":"1987","unstructured":"Delli-Bovi PD, Curatola AM, Kern FG. An oncogene isolated by transfection of Kaposi's sarcoma DNA encodes a growth factor that is a member of the FGF family. Cell 1987;50:729-37.","journal-title":"Cell"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1002\/jcp.1041170310","article-title":"Abnormal behaviour of cultured fibroblasts from nodule and non-affected aponeurosis of Dupuytren's disease","volume":"117","author":"Azzarone, B.; Failly-Crepin, C.; Daya-Grosjean, L.; ChaponNeir, C.; Gabbiani, C.","year":"1983","unstructured":"Azzarone B, Failly-Crepin C, Daya-Grosjean L, ChaponNeir C, Gabbiani C. Abnormal behaviour of cultured fibroblasts from nodule and non-affected aponeurosis of Dupuytren's disease. Y Cel Physiol 1983;117:353-61.","journal-title":"Y Cel Physiol"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1125","DOI":"10.1016\/0006-291X(87)90525-0","article-title":"Isolation of a myofibroblast growth factor from human breast carcinoma cell lines","volume":"149","author":"Barsky, S.H.; Gopalkrishna, R.","year":"1987","unstructured":"Barsky SH, Gopalkrishna R. Isolation of a myofibroblast growth factor from human breast carcinoma cell lines. Biochem Biophys Res Comm 1987;149:1125-31.","journal-title":"Biochem Biophys Res Comm"},{"key":"ref_10","first-page":"89","article-title":"Detection of gene expression","author":"Kawasaki, E.S.; Wang, A.M.","year":"1989","unstructured":"Kawasaki ES, Wang AM. Detection of gene expression. In: Erlich HA, ed. PCR technology. New York: Stockton Press, 1989:89-98."},{"key":"ref_11","first-page":"156","article-title":"Single-step method of RNA isolation by acid guanidinium thiocyanate-phenolchloroform extraction","volume":"162","author":"Chomczynski, P.; Sacchi, N.","year":"1987","unstructured":"Chomczynski P, Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenolchloroform extraction. Anal Biochem 1987;162:156-9.","journal-title":"Anal Biochem"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1038\/339237a0","article-title":"Avoiding false positives with PCR","volume":"339","author":"Kwok, S.; Higuchi, R.","year":"1989","unstructured":"Kwok S, Higuchi R Avoiding false positives with PCR. Nature 1989;339:237-8.","journal-title":"Nature"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"387","DOI":"10.1093\/nar\/12.1Part1.387","article-title":"Smithies 0. A comprehensive set of sequence analysis programs for the VAX","volume":"12","author":"Deveraux, J.; Haeberli, P.","year":"1984","unstructured":"Deveraux J, Haeberli P, Smithies 0. A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res 1984;12:387-95.","journal-title":"Nucleic Acids Res"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1084\/jem.174.2.493","article-title":"Cytokine gene transcription in vascularised organ grafts: analysis using semiquantitative polymerase chain reaction","volume":"174","author":"DalIman, M.J.; Larsen, C.P.; Morris, P.J.","year":"1991","unstructured":"DalIman MJ, Larsen CP, Morris PJ. Cytokine gene transcription in vascularised organ grafts: analysis using semiquantitative polymerase chain reaction. J Exp Med 1991;174:493-6.","journal-title":"J Exp Med"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"708","DOI":"10.1126\/science.3041594","article-title":"Wound macrophages express TGF alpha and other growth factors in vivo: analysis by mRNA phenotyping","volume":"24","author":"Rappolee, D.A.; Mark, D.J.; Banda, M.J.; Werb, Z.","year":"1988","unstructured":"Rappolee DA, Mark DJ, Banda MJ, Werb Z. Wound macrophages express TGF alpha and other growth factors in vivo: analysis by mRNA phenotyping. Science 1988;24:708-12.","journal-title":"Science"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"995","DOI":"10.1002\/art.1780270905","article-title":"Induction of fibroblast proliferation by interleukin-I derived from human monocytic leukemia cells","volume":"27","author":"Postlethwaite, A.E.; Lachman, L.B.; Kang, A.H.","year":"1984","unstructured":"Postlethwaite AE, Lachman LB, Kang AH. Induction of fibroblast proliferation by interleukin-I derived from human monocytic leukemia cells. Arthritis Rheum 1984; 27:995-1001.","journal-title":"Arthritis Rheum"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1016\/0167-5699(90)90005-T","article-title":"Interleukin- 1: the first interleulin","volume":"11","author":"DiGiovine, F.S.; Duff, G.W.","year":"1990","unstructured":"DiGiovine FS, Duff GW. Interleukin-1: the first interleulin. Immunology Today 1990;11:13-20.","journal-title":"Immunology Today"},{"key":"ref_18","first-page":"231","article-title":"Fibroblast growth factor: Chemical structure and biologic function","volume":"257","author":"Gospodarowicz, D.","year":"1990","unstructured":"Gospodarowicz D. Fibroblast growth factor: Chemical structure and biologic function. Clin Orthop Rel Res 1990;257:231-48.","journal-title":"Clin Orthop Rel Res"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"467","DOI":"10.1111\/j.1432-1033.1990.tb15327.x","article-title":"Transforming growth factors and the regulation of cell proliferation","author":"Lyons, R.M.; Moses, H.L.","year":"1990","unstructured":"Lyons RM, Moses HL. Transforming growth factors and the regulation of cell proliferation. Eur J Biochem 1990; 187:467-73.","journal-title":"Eur J Biochem"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1179","DOI":"10.1016\/S0140-6736(89)92760-8","article-title":"Platelet-derived growth factor","volume":"i:","author":"Ross, R.","year":"1989","unstructured":"Ross R Platelet-derived growth factor. Lancet 1989;i: 1179-82.","journal-title":"Lancet"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"2453","DOI":"10.1073\/pnas.83.8.2453","article-title":"Induction of c-sis mRNA and activity similar to platelet-derived growth factor by transforming growth factor beta: A proposed model for indirect mitogenesis involving autocrine activity","volume":"83","author":"Leof, E.B.; Proper, J.A.; Goustin, A.S.; Shipley, G.D.; DiCorletto, P.E.; Moses, H.L.","year":"1986","unstructured":"Leof EB, Proper JA, Goustin AS, Shipley GD, DiCorletto PE, Moses HL. Induction of c-sis mRNA and activity similar to platelet-derived growth factor by transforming growth factor beta: A proposed model for indirect mitogenesis involving autocrine activity. l+oc Nail Acad Sci USA 1986;83:2453-7.","journal-title":"l+oc Nail Acad Sci USA"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1084\/jem.165.1.251","article-title":"Stimulation of the chemotactic migration of human fibroblasts by transforming growth factor beta","volume":"165","author":"Posdethwaite, A.E.; Keski-Oja, J.; Moses, H.L.; Kang, A.H.","year":"1987","unstructured":"Posdethwaite AE, Keski-Oja J, Moses HL, Kang AH. Stimulation of the chemotactic migration of human fibroblasts by transforming growth factor beta. J Exp Med 1987;165:251-6.","journal-title":"J Exp Med"},{"key":"ref_23","first-page":"6443","article-title":"Regulation of fibronectin and type I collagen mRNA levels by transforming growth factor beta.3Biol","volume":"262","author":"Ignotz, R.A.; Endo, T.; Massague, J.","year":"1987","unstructured":"Ignotz RA, Endo T, Massague J. Regulation of fibronectin and type I collagen mRNA levels by transforming growth factor beta.3Biol Chem 1987;262:6443-6.","journal-title":"Chem"},{"key":"ref_24","first-page":"4167","article-title":"Transforming growth factor type beta: Rapid induction of fibrosis and angiogenesis in vivo and stimulation of collagen","volume-title":"formation in vitro. Proc Nad Acad Sci","volume":"83","author":"Roberts, A.B.; Sporn, M.B.; Assoian, R.K.","year":"1986","unstructured":"Roberts AB, Sporn MB, Assoian RK, et al. Transforming growth factor type beta: Rapid induction of fibrosis and angiogenesis in vivo and stimulation of collagen formation in vitro. Proc Nad Acad Sci USA 1986;83:4167-71."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1016\/0266-7681(91)90051-O","article-title":"An investigation into the role of inflammatory cells in Dupuytren's disease","volume":"16","author":"Andrew, J.G.; Andrew, S.M.; Tumer, B.","year":"1991","unstructured":"Andrew JG, Andrew SM, Tumer B. An investigation into the role of inflammatory cells in Dupuytren's disease. J Hand Surg 1991;16B:267-71.","journal-title":"J Hand Surg"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1172\/JCI112815","article-title":"Secretory products of macrophages","volume":"79","author":"Nathan, C.F.","year":"1987","unstructured":"Nathan CF. Secretory products of macrophages. J Clin Invest 1987;79:319-26.","journal-title":"J Clin Invest"}],"container-title":["Journal of Clinical Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1136\/jcp.46.5.425","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,14]],"date-time":"2025-12-14T15:17:07Z","timestamp":1765725427000},"score":1,"resource":{"primary":{"URL":"https:\/\/jcp.bmj.com\/lookup\/doi\/10.1136\/jcp.46.5.425"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1993,5]]},"references-count":26,"journal-issue":{"issue":"5","published-online":{"date-parts":[[1993,5,1]]},"published-print":{"date-parts":[[1993,5]]}},"alternative-id":["10.1136\/jcp.46.5.425"],"URL":"https:\/\/doi.org\/10.1136\/jcp.46.5.425","relation":{},"ISSN":["0021-9746","1472-4146"],"issn-type":[{"value":"0021-9746","type":"print"},{"value":"1472-4146","type":"electronic"}],"subject":[],"published":{"date-parts":[[1993,5]]}}}