{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T06:13:39Z","timestamp":1791094419441,"version":"4.1.0"},"reference-count":24,"publisher":"BMJ","issue":"7","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clin Pathol"],"published-print":{"date-parts":[[1993,7]]},"abstract":"<jats:p>AIM--To develop a polymerase chain reaction (PCR) for the specific detection of the C protein gene in strains of group B Streptococcus. METHODS--A single primer pair derived from the nucleotide sequence of the IgA binding beta antigen of the C protein complex permitted the specific amplification of a 592 base pair DNA fragment from the C protein gene. After 35 cycles of amplification this product could be detected by agarose gel electrophoresis. Southern blot hybridisation confirmed that this product was the C protein gene. RESULTS--PCR detected the C protein gene in 75 (63%) of 119 strains of group B streptococci analysed. The product was not detected in other Gram positive organisms, showing that this PCR assay was highly specific. The sensitivity of the assay was satisfactory to a dilution of 1 in 10,000 of extracted DNA. CONCLUSIONS--The C protein of group B streptococci is associated with neonatal sepsis. The specific detection of the C protein gene by PCR may help identify which strains are likely to be associated with infection by the organism.<\/jats:p>","DOI":"10.1136\/jcp.46.7.633","type":"journal-article","created":{"date-parts":[[2007,6,9]],"date-time":"2007-06-09T00:50:46Z","timestamp":1181350246000},"page":"633-636","source":"Crossref","is-referenced-by-count":15,"title":["Detection of the C protein gene among group B streptococci using PCR."],"prefix":"10.1136","volume":"46","author":[{"given":"J A","family":"Mawn","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A J","family":"Simpson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S R","family":"Heard","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"239","published-online":{"date-parts":[[1993,7,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1093\/infdis\/139.2.147","article-title":"Electron microscopic definition of surface antigens of Group B Streptococcus","volume":"139","author":"Kasper, D.L.; Baker, C.S.","year":"1979","unstructured":"Kasper DL, Baker CS. 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Reduction in morbidity and mortality rates for neonatal group B streptococcal disease through early diagnosis and prophylaxis. I ClinMicrobiol 1986;23:489-92."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"812","DOI":"10.1302\/0301-620X.70B5.3056946","article-title":"Skeletal infection by Group B streptococcus in neonates. A case report and review of literature","volume":"70","author":"Baxter, M.P.; Finnegan, M.A.","year":"1988","unstructured":"Baxter MP, Finnegan MA. Skeletal infection by Group B streptococcus in neonates. A case report and review of literature. J Bone joint Surg 1988;70:812-14.","journal-title":"J Bone joint Surg"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"919","DOI":"10.1016\/S0022-3476(73)80524-4","article-title":"Transmission of Group B streptococcus among parturient women and their neonates","volume":"83","author":"Baker, C.J.; Barrett, F.F.","year":"1973","unstructured":"Baker CJ, Barrett FF. 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Rev Med Microbiol 1992;3:52-8.","journal-title":"Rev Med Microbiol"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1136\/jcp.46.2.145","article-title":"a new phenotypic typing scheme","volume":"46","author":"Heard, SR, Mawn J.M.Group B.streptococcus","year":"1993","unstructured":"Heard SR, Mawn JM. Group B streptococcus, a new phenotypic typing scheme. J Clin Pathol 1993;46: 145-8.","journal-title":"J Clin Pathol"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1084\/jem.142.1.165","article-title":"Multiple mouse protective antibodies effective against protein antigens","volume":"142","author":"Lancefield, R.C.; McCarty, M.; Everly, W.N.","year":"1975","unstructured":"Lancefield RC, McCarty M, Everly WN. Multiple mouse protective antibodies effective against protein antigens. J Exp Med 1975;142:165-79.","journal-title":"J Exp Med"},{"key":"ref_10","first-page":"121","article-title":"Mouse protective antibodies against the lbc proteins of group B streptococci. Acta Pathol Microbiol Immunol Scand 1985; 93 Sect B","author":"Bevanger, L.; Naess, A.I.","unstructured":"Bevanger L, Naess AI. Mouse protective antibodies against the lbc proteins of group B streptococci. Acta Pathol Microbiol Immunol Scand 1985;93 Sect B: 121-4."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1016\/0882-4010(86)90021-5","article-title":"Isolation of a C(lbc) protein from a Group B streptococcus which elicits mouse protective antibody","volume":"l:","author":"Valtonen, M.V.; Kasper, D.L.; Levy, N.J.","year":"1986","unstructured":"Valtonen MV, Kasper DL, Levy NJ. Isolation of a C(lbc) protein from a Group B streptococcus which elicits mouse protective antibody. Microbiol Pathol 1986;l: 191-204.","journal-title":"Microbiol Pathol"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"4989","DOI":"10.1128\/IAI.60.12.4989-4994.1992","article-title":"Protection of neonatal mice from group B streptococcal infection by maternal immunisation from beta 'C' protein","volume":"60","author":"Madoff, L.C.; Michel, J.L.; Gong, E.W.; Rodewald, A.K.; Kasper, D.L.","year":"1992","unstructured":"Madoff LC, Michel JL, Gong EW, Rodewald AK, Kasper DL. Protection of neonatal mice from group B streptococcal infection by maternal immunisation from beta 'C' protein. Infect Immun 1992;60:4989-94.","journal-title":"Infect Immun"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"596","DOI":"10.1128\/IAI.4.5.596-604.1971","article-title":"Typing specific antigens of Group B type Ic streptococci","volume":"4","author":"Wilkinson, H.W.; Eagon, R.G.","year":"1991","unstructured":"Wilkinson HW, Eagon RG. Typing specific antigens of Group B type Ic streptococci. Infect Immun 1991;4:596-604.","journal-title":"Infect Immun"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"2023","DOI":"10.1128\/IAI.59.6.2023-2028.1991","article-title":"Cloned alpha and beta 'C' protein antigens of Group B streptococci elicit protective immunity","volume":"59","author":"Michel, J.L.; Madoff, L.C.; Kling, D.E.; Kasper, D.L.; Ausobel, F.M.","year":"1991","unstructured":"Michel JL, Madoff LC, Kling DE, Kasper DL, Ausobel FM. Cloned alpha and beta 'C' protein antigens of Group B streptococci elicit protective immunity. Infect Immun 1991;59:2023-8.","journal-title":"Infect Immun"},{"key":"ref_15","first-page":"786","article-title":"Group B streptococcal 'C' protein associated antigens; association with neonatal sepsis. J9 Infect","volume":"163","author":"Chun, C.S.Y.; Brady, U.; Boyle, M.D.P.; Dillon, H.C.; Ayoub, E.M.","year":"1991","unstructured":"Chun CSY, Brady U, Boyle MDP, Dillon HC, Ayoub EM. Group B streptococcal 'C' protein associated antigens; association with neonatal sepsis. J9 Infect Dis 1991;163:786-91.","journal-title":"Dis"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"843","DOI":"10.1111\/j.1365-2958.1991.tb00757.x","article-title":"The IgA binding beta antigen for the C protein complex of Group B streptococci: Sequence determination of its gene and detection of two binding regions","volume":"5","author":"Jerlstrom, P.G.; Chhatwal, G.S.; Timmis, K.A.","year":"1991","unstructured":"Jerlstrom PG, Chhatwal GS, Timmis KA. The IgA binding beta antigen for the C protein complex of Group B streptococci: Sequence determination of its gene and detection of two binding regions. Mol Microbiol 1991;5:843-9.","journal-title":"Mol Microbiol"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1126\/science.239.4839.487","article-title":"Primer directed enzymatic amplification of DNA with a thermostable DNA polymerase","volume":"239","author":"Saiki, R.K.; Gelfand, D.; Stoffel, S.","year":"1988","unstructured":"Saiki RK, Gelfand D, Stoffel S, et al. Primer directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 1988;239:487-9 1.","journal-title":"Science"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"977","DOI":"10.1093\/infdis\/161.5.977","article-title":"Bates SH, Crawford JT. Polymerase chain reaction amplification of a repetitive DNA sequence for Mycobacterium tuberculosis","volume":"161","year":"1990","unstructured":"Eisenach KD, Case MD, Bates SH, Crawford JT. Polymerase chain reaction amplification of a repetitive DNA sequence for Mycobacterium tuberculosis. J Infect Dis 1990;161:977-81.","journal-title":"J Infect Dis"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1038\/339237a0","article-title":"Avoiding false positives with PCR","volume":"339","author":"Kwock, S.; Higuchi, R.","year":"1989","unstructured":"Kwock S, Higuchi R. Avoiding false positives with PCR. Nature 1989;339:237-8.","journal-title":"Nature"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"503","DOI":"10.1016\/S0022-2836(75)80083-0","article-title":"Detection of specific sequences among DNA fragments separated by gel electrophoresis","volume":"98","author":"Southern, E.M.","year":"1975","unstructured":"Southern EM. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 1975;98:503-17.","journal-title":"J Mol Biol"},{"key":"ref_21","first-page":"175","article-title":"Detection of Legionella with polymerase chain reaction and gene probe methods","volume":"4","author":"Mahbubani, M.H.; Bej, A.K.; Miller, R.; Haff, L.; Dicesare, J.; Atlas, R.M.","year":"1990","unstructured":"Mahbubani MH, Bej AK, Miller R, Haff L, Dicesare J, Atlas RM. Detection of Legionella with polymerase chain reaction and gene probe methods. Mol Cell I-robes 1990;4:175-87.","journal-title":"Mol Cell I-robes"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1093\/infdis\/136.1.137","article-title":"Summary of the workshop on pernatal infections due to Group B Streptococcus","volume":"136","author":"Baker, C.J.","year":"1977","unstructured":"Baker CJ. Summary of the workshop on pernatal infections due to Group B Streptococcus. J Infect Dis 1977;136:137-52.","journal-title":"J Infect Dis"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"356","DOI":"10.1093\/cid\/10.Supplement_2.S363","article-title":"Surface localised protein antigens of Group B streptococci","volume":"10","author":"Ferrieri, P.","year":"1988","unstructured":"Ferrieri P. Surface localised protein antigens of Group B streptococci. Rev Infect Dis 1988;10:S356-9.","journal-title":"Rev Infect Dis"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1467","DOI":"10.1084\/jem.160.5.1467","article-title":"A surface receptor specific for human IgA on Group B streptococci possessing the lbc protein antigen","volume":"160","author":"Russell-Jones, G.J.; Gotschlich, E.C.; Blake, M.S.","year":"1984","unstructured":"Russell-Jones GJ, Gotschlich EC, Blake MS. A surface receptor specific for human IgA on Group B streptococci possessing the lbc protein antigen. 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