{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,14]],"date-time":"2025-12-14T09:40:09Z","timestamp":1765705209690,"version":"3.48.0"},"reference-count":29,"publisher":"BMJ","issue":"10","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clin Pathol"],"published-print":{"date-parts":[[1994,10]]},"abstract":"<jats:p>AIMS--To evaluate the presence of cytomegalovirus (CMV) DNA in the cerebrospinal fluid of patients with AIDS and suspected viral encephalitis using an in situ hybridisation assay with digoxigenin labelled CMV DNA probes. METHODS--The presence of CMV DNA was evaluated in cerebrospinal fluid cells of 10 patients with AIDS using in situ hybridisation. The positivity of CMV DNA was confirmed by the presence of CMV induced antigens in the same specimens. The presence of CMV DNA and CMV induced antigens was also analysed in peripheral blood leucocytes. The time required to perform the in situ hybridisation assay was about eight hours. RESULTS--The in situ hybridisation assay was sensitive, specific, and provided good resolution. Six patients proved positive for the presence of CMV DNA in CSF cells and all six also proved positive for CMV DNA in blood leucocytes. Of the six CMV positive patients, five were treated with specific antiviral drugs: of these, one died during the treatment while four clinically recovered after one month of treatment. CONCLUSIONS--The in situ hybridisation assay using digoxigenin labelled CMV DNA probes can be used as a valuable diagnostic test for the detection of CMV DNA in the cerebrospinal fluid cells of patients with suspected CMV encephalitis and can therefore prompt adequate antiviral therapeutic intervention.<\/jats:p>","DOI":"10.1136\/jcp.47.10.886","type":"journal-article","created":{"date-parts":[[2007,6,9]],"date-time":"2007-06-09T00:50:46Z","timestamp":1181350246000},"page":"886-891","source":"Crossref","is-referenced-by-count":11,"title":["Rapid diagnosis of cytomegalovirus encephalitis in patients with AIDS using in situ hybridisation."],"prefix":"10.1136","volume":"47","author":[{"given":"M","family":"Musiani","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M","family":"Zerbini","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S","family":"Venturoli","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G","family":"Gentilomi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"V","family":"Borghi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P","family":"Pietrosemoli","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M","family":"Pecorari","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M","family":"La Placa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[1994,10,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"608","DOI":"10.1093\/clinids\/14.2.608-a","article-title":"Cytomegalovirus infection in patients with AIDS","volume":"14","author":"Drew, W.L.","year":"1992","unstructured":"Drew WL. Cytomegalovirus infection in patients with AIDS. J Clin Infect Dis 1992;14:608-15.","journal-title":"J Clin Infect Dis"},{"key":"ref_2","first-page":"147","article-title":"Infection in the renal transplant recipient","author":"Rubin, R.H.","year":"1989","unstructured":"Rubin RH. Infection in the renal transplant recipient. In: Brenner BM, Stein JH, Milford EL, eds. Renal transplantation. New York: Churchill Livingstone, 1989:147-80."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1002\/ana.410140404","article-title":"Neurological complication of acquired immune deficiency syndrome: Analysis of 50 patients","volume":"14","author":"Snider, W.D.; Simpson, D.M.; Nielsen, S.; Gold, J.W.M.; Metroka, C.E.; Posner, J.B.","year":"1983","unstructured":"Snider WD, Simpson DM, Nielsen S, Gold JWM, Metroka CE, Posner JB. Neurological complication of acquired immune deficiency syndrome: Analysis of 50 patients. Ann Neurol 1983;14:403-18.","journal-title":"Ann Neurol"},{"key":"ref_4","first-page":"275","article-title":"Cytomegalic AIDS: CT, inclusion virus encephalitis in patients with clinical and pathological correlations","volume":"7","author":"MJ, Donovan Post; GT, Hensley; LB, Moskowitz; M, Fischl","year":"1986","unstructured":"Donovan Post MJ, Hensley GT, Moskowitz LB, Fischl M. Cytomegalic AIDS: CT, inclusion virus encephalitis in patients with clinical and pathological correlations Am J Neuroradiol 1986;7:275-80.","journal-title":"Am J Neuroradiol"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1093\/brain\/112.1.245","article-title":"Cytomegalovirus in the nervous system of patients with acquired immune deficiency syndrome (AIDS)","volume":"112","author":"Vinters, H.V.; Kwok, M.K.; Ho, M.W.; Anders, K.H.; Tomiyasu, U.; Wolfson, W.I.I.","year":"1989","unstructured":"Vinters HV, Kwok MK, Ho MW, Anders KH, Tomiyasu U, Wolfson WII, et al. Cytomegalovirus in the nervous system of patients with acquired immune deficiency syndrome (AIDS). Brain 1989;112:245-68.","journal-title":"Brain"},{"key":"ref_6","first-page":"423","article-title":"Diagnostic virology","author":"Mcintosh, K.","year":"1990","unstructured":"Mcintosh K. Diagnostic virology. In: Fields BN, Knipe DM, eds. Virology. 2nd edn. New York: Raven Press, 1990:423-4.","edition":"2nd edn"},{"key":"ref_7","first-page":"2101","volume":"28","author":"Musiani, M.; Zerbini, M.; Gentilomi, G.; Gallinella, G.; Venturoli, S.; Gibellimi, D.","year":"1990","unstructured":"Musiani M, Zerbini M, Gentilomi G, Gallinella G, Venturoli S, Gibellimi D, et al. Rapid detection of hcyybtroimdeigzaaltoivoinruismmDuNnoAeniznyumraitniecsaamsspalye.sJwiCtlhinaMdiocrtobbliootl 1990;28:2101-3.","journal-title":"Rapid detection of hcyybtroimdeigzaaltoivoinruismmDuNnoAeniznyumraitniecsaamsspalye.sJwiCtlhinaMdiocrtobbliootl"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1016\/0022-1759(89)90091-4","article-title":"La Placa M. A hybrido-immunocytochemical assay for the in situ detection of cytomegalovirus DNA using digoxigenin-labeled probes","volume":"125","author":"Gentilomi, G.; Musiani, M.; Zerbini, M.; Gallinella, G.; Gibellini, D.","year":"1989","unstructured":"Gentilomi G, Musiani M, Zerbini M, Gallinella G, Gibellini D, La Placa M. A hybrido-immunocytochemical assay for the in situ detection of cytomegalovirus DNA using digoxigenin-labeled probes. Y Immunol Methods 1989;125:177-83.","journal-title":"Y Immunol Methods"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1007\/BF00266785","article-title":"In situ detection of cytomegalovirus DNA in biopsies of AIDS patients using a hybrido-immunocytochemical assay","volume":"94","author":"Musiani, M.; Gentilomi, G.; Zerbini, M.; Gibellini, D.; Gallinella, G.; Pileri, S.","year":"1990","unstructured":"Musiani M, Gentilomi G, Zerbini M, Gibellini D, Gallinella G, Pileri S, et al. In situ detection of cytomegalovirus DNA in biopsies of AIDS patients using a hybrido-immunocytochemical assay. Histochemistry 1990;94:21-5.","journal-title":"Histochemistry"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1097\/00005072-198603000-00003","article-title":"Localization within the CNS of cytomegalovirus proteins and genome during fulminant infection in an AIDS patient","volume":"45","author":"Wiley, C.A.; Schrier, R.D.; Denaro, F.J.; Nelson, J.A.; Lampert, P.W.; Oldstone, M.B.A.","year":"1986","unstructured":"Wiley CA, Schrier RD, Denaro FJ, Nelson JA, Lampert PW, Oldstone MBA. Localization within the CNS of cytomegalovirus proteins and genome during fulminant infection in an AIDS patient. J Neuropathol Exp Neurol 1986;45:127-9.","journal-title":"J Neuropathol Exp Neurol"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1002\/jmv.1890290204","article-title":"Detection of human cytomegalovirus immediate early antigen in leukocytes as a marker of viremia in immunocompromised patients","volume":"29","author":"Revello, M.G.; Percivalle, E.; Zavattoni, M.; Parea, M.; Grossi, P.; Gema, G.","year":"1989","unstructured":"Revello MG, Percivalle E, Zavattoni M, Parea M, Grossi P, Gema G. Detection of human cytomegalovirus immediate early antigen in leukocytes as a marker of viremia in immunocompromised patients. Jf Med Virol 1989;29:88-93.","journal-title":"Jf Med Virol"},{"key":"ref_12","first-page":"39","article-title":"Structural organization of the DNA molecules from human cytomegalovirus","volume-title":"virus genetics","author":"RL, La Femina; GS, Hayward","year":"1980","unstructured":"La Femina RL, Hayward GS. Structural organization of the DNA molecules from human cytomegalovirus. In: Fields BN, Jaenisch R, eds. Animal virus genetics. New York: Academic Press, 1980:39-55."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1016\/0378-1119(81)90077-9","article-title":"Cloning of the human cytomegalovirus genome as Xba I endonuclease fragments","volume":"16","author":"Thomsen, D.R.; Stinski, M.F.","year":"1981","unstructured":"Thomsen DR, Stinski MF. Cloning of the human cytomegalovirus genome as Xba I endonuclease fragments. Gene 1981;16:207-16.","journal-title":"Gene"},{"key":"ref_14","article-title":"Molecular cloning: a laboratory manual. Cold Spring Harbor","author":"Sambrook, J.; Fritsch, E.F.; Maniatis, T.","year":"1989","unstructured":"Sambrook J, Fritsch EF, Maniatis T. Molecular cloning: a laboratory manual. 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Chemiluminescent assay for the detection of viral and plasmid DNA using digoxigeninlabelled probes. Analyt Biochem 1991;194:394-8.","journal-title":"Analyt Biochem"},{"key":"ref_17","first-page":"125","article-title":"Detection of B 19 parvovirus infections by a dot -blot hybridization assay using a digoxigenin-labelled probe. J7 Virol","volume":"27","author":"Azzi, A.; Zakrewska, K.; Gentilomi, G.; Musiani, M.; Zerbini, M.","year":"1990","unstructured":"Azzi A, Zakrewska K, Gentilomi G, Musiani M, Zerbini M. Detection of B 19 parvovirus infections by a dot -blot hybridization assay using a digoxigenin-labelled probe. J7 Virol Methods 1990;27:125-34.","journal-title":"Methods"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1016\/0166-0934(89)90046-3","article-title":"Detection of cytomegalovirus DNA using probes labelled with digoxigenin","volume":"24","author":"Kimpton, C.P.; Corbitt, G.; Morris, D.J.","year":"1989","unstructured":"Kimpton CP, Corbitt G, Morris DJ. Detection of cytomegalovirus DNA using probes labelled with digoxigenin. _J Virol Methods 1989;24:335-46.","journal-title":"_J Virol Methods"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1177\/40.3.1313062","article-title":"Double in situ hybridization for detection of herpes simplex virus and cytomegalovirus DNA using non radioactive probes","volume":"40","author":"Gentilomi, G.; Musiani, M.; Zerbini, M.; Gibellini, D.; Gallinella, G.; Venturoli, S.","year":"1992","unstructured":"Gentilomi G, Musiani M, Zerbini M, Gibellini D, Gallinella G, Venturoli S. Double in situ hybridization for detection of herpes simplex virus and cytomegalovirus DNA using non radioactive probes. J Histochem Cytochem 1992;40:421-5.","journal-title":"J Histochem Cytochem"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"829","DOI":"10.1177\/20.10.829","article-title":"Inhibition of erythrocyte pseudoperoxidase activity by treatment with hydrogen peroxide following methanol","volume":"20","author":"Streefkerk, J.G.","year":"1972","unstructured":"Streefkerk JG. Inhibition of erythrocyte pseudoperoxidase activity by treatment with hydrogen peroxide following methanol. Jf Histochem Cytochem 1972;20:829-34.","journal-title":"Jf Histochem Cytochem"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1177\/22.1.51","article-title":"Destruction of endogenous peroxidase activity in order to locate cellular antigens by peroxidase labeled antibody","volume":"22","author":"Weir, E.E.; Pretlow, T.G.; Pitts, A.; William, E.E.","year":"1974","unstructured":"Weir EE, Pretlow TG, Pitts A, William EE. Destruction of endogenous peroxidase activity in order to locate cellular antigens by peroxidase labeled antibody. J. Histochem Cytochem 1974;22:51-6.","journal-title":"J. Histochem Cytochem"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1212\/WNL.23.2.136","article-title":"Cytomegalovirus encephalitis in adults","volume":"23","author":"Dorfman, U.","year":"1973","unstructured":"Dorfman U. Cytomegalovirus encephalitis in adults. Neurology 1973;23:136-44.","journal-title":"Neurology"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"678","DOI":"10.1093\/ajcp\/82.6.678","article-title":"Subacute encephalitis in acquired immune deficiency syndrome: a postmortem study","volume":"82","author":"Nielsen, S.L.; Petito, C.K.; Urmacher, C.D.; Posner, J.B.","year":"1984","unstructured":"Nielsen SL, Petito CK, Urmacher CD, Posner JB. Subacute encephalitis in acquired immune deficiency syndrome: a postmortem study. Am Jf Clin Pathol 1984; 82:678-82.","journal-title":"Am Jf Clin Pathol"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1136\/thx.44.4.241","article-title":"Human cytomegalovirus infection","volume":"44","author":"Sissons, J.G.P.","year":"1989","unstructured":"Sissons JGP. Human cytomegalovirus infection. Thorax 1989;44:241-6.","journal-title":"Thorax"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1093\/infdis\/161.1.31","article-title":"Localization of human cytomegalovirus in peripheral blood leukocytes by in situ hybridization","volume":"161","author":"Dankner, W.E.; McCutchan, J.A.; Richman, D.D.; Hirata, K.; Spector, S.A.","year":"1990","unstructured":"Dankner WE, McCutchan JA, Richman DD, Hirata K, Spector SA. Localization of human cytomegalovirus in peripheral blood leukocytes by in situ hybridization. J Infect Dis 1990;161:31-6.","journal-title":"J Infect Dis"},{"key":"ref_26","first-page":"644","article-title":"Viremia and glial nodule encephalitis after experimental systemic cytomegalovirus infection","volume":"61","author":"Booss, J.; Winkler, SR, Griffith; BF, Kim; J.H.","year":"1989","unstructured":"Booss J, Winkler SR, Griffith BF, Kim JH. Viremia and glial nodule encephalitis after experimental systemic cytomegalovirus infection. Lab Invest 1989;61:644-9.","journal-title":"Lab Invest"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1412","DOI":"10.1093\/infdis\/166.6.1412","article-title":"Diagnosis of human cytomegalovirus central nervous system disease in AIDS patients by DNA amplification from cerebrospinal fluid","volume":"166","author":"Wolf, D.G.; Spector, S.A.","year":"1992","unstructured":"Wolf DG, Spector SA. Diagnosis of human cytomegalovirus central nervous system disease in AIDS patients by DNA amplification from cerebrospinal fluid. _l Infect Dis 1992;166:1412-5.","journal-title":"_l Infect Dis"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1416","DOI":"10.1093\/infdis\/166.6.1416","article-title":"Rapid detection of cytomegalovirus DNA in cerebrospinal fluid of AIDS patients with neurological disorders","volume":"166","author":"true","year":"1992","unstructured":"Gozlan J, Salord JM, Roullet E, Baudrimont M, Caburet F, Picard 0, et al. Rapid detection of cytomegalovirus DNA in cerebrospinal fluid of AIDS patients with neurological disorders. Jf Infect Dis 1992; 166: 1416-21.","journal-title":"Jf Infect Dis"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1408","DOI":"10.1093\/infdis\/166.6.1408","article-title":"Cytomegalovirus infection of the central nervous system in patients with AIDS: diagnosis by DNA amplification from cerebrospinal fluid","volume":"166","author":"Cinque, P.; Vago, L.; Brytting, M.; Castagna, A.; Accordini, A.; Sundqvist, V.A.","year":"1992","unstructured":"Cinque P, Vago L, Brytting M, Castagna A, Accordini A, Sundqvist VA, et al. Cytomegalovirus infection of the central nervous system in patients with AIDS: diagnosis by DNA amplification from cerebrospinal fluid. 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