{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T15:14:01Z","timestamp":1770822841219,"version":"3.50.1"},"reference-count":10,"publisher":"BMJ","issue":"12","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clin Pathol"],"published-print":{"date-parts":[[1994,12]]},"abstract":"<jats:p>AIM--To prepare a monoclonal antibody (EB9) against the trophozoite form of Pneumocystis carinii and to test its efficacy for detecting infection with this organism. METHOD--The sensitivity and specificity of the EB9 antibody were assessed by comparing it with other conventional stains (Papanicolaou, Giemsa and Grocott) and 3F6 antibody in 33 bronchoalveolar lavage specimens from HIV positive patients suspected of having P carinii pneumonia. RESULTS--P carinii infection was detected in 15 of 33 patients. In 14 cases the organism was detected by two or more of the staining methods used; however, EB9 failed to detect infection in two cases which were positive by other staining techniques. In one case P carinii infection was detected by EB9 only. CONCLUSION--The results of this study suggest that P carinii infection in the lung may occur in two forms: the cyst form and the trophozoite form, which may explain the observed variation in response to treatment.<\/jats:p>","DOI":"10.1136\/jcp.47.12.1108","type":"journal-article","created":{"date-parts":[[2007,6,9]],"date-time":"2007-06-09T00:50:46Z","timestamp":1181350246000},"page":"1108-1111","source":"Crossref","is-referenced-by-count":5,"title":["EB9, a new antibody for the detection of trophozoites of Pneumocystis carinii in bronchoalveolar lavage specimens in AIDS."],"prefix":"10.1136","volume":"47","author":[{"given":"J F","family":"Wazir","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I","family":"Brown","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E","family":"Martin-Bates","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D V","family":"Coleman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[1994,12,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1111\/j.1365-2303.1994.tb00531.x","article-title":"Evaluation of the sensitivity, specificity, and predictive value of monoclonal antibody 3F6 for the detection of Pneumocystis carinii pneumonia in bronchoalveolar lavage specimens and induced sputum","volume":"5","author":"Wazir, J.F.; Macrorie, S.G.; Coleman, D.V.","year":"1994","unstructured":"Wazir JF, Macrorie SG, Coleman DV. Evaluation of the sensitivity, specificity, and predictive value of monoclonal antibody 3F6 for the detection of Pneumocystis carinii pneumonia in bronchoalveolar lavage specimens and induced sputum. Cytopathology 1994;5:82-9.","journal-title":"Cytopathology"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/0022-1759(87)90435-2","article-title":"Detection of Pneumocystis carinii in lung-derived samples using monoclonal antibodies to an 82 KDa parasite component","volume":"98","author":"Linder, E.; Lundin, L.; Vorma, H.","year":"1987","unstructured":"Linder E, Lundin L, Vorma H. Detection of Pneumocystis carinii in lung-derived samples using monoclonal antibodies to an 82 KDa parasite component. 7 Immunol Methods 1987;98:57-62.","journal-title":"7 Immunol Methods"},{"key":"ref_3","first-page":"129","article-title":"Enzyme linked immunosorbent assay, ELISA. III. Quantitation of specific antibodies by enzyme-labelled anti-immunoglobulin in antigen-coated tubes","volume":"109","author":"Engvall, E.; Perlmann, P.","year":"1972","unstructured":"Engvall E, Perlmann P. Enzyme linked immunosorbent assay, ELISA. III. Quantitation of specific antibodies by enzyme-labelled anti-immunoglobulin in antigen-coated tubes. Immunol 1972;109:129-35.","journal-title":"Immunol"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1111\/j.1365-2303.1991.tb00390.x","article-title":"Evaluation of immunocytochemical staining as a method of improving diagnostic accuracy in a routine cytopathology laboratory","volume":"2","author":"Wazir, J.F.; Martin-Bates, E.; Woodward, G.; Coleman, D.V.","year":"1991","unstructured":"Wazir JF, Martin-Bates E, Woodward G, Coleman DV. Evaluation of immunocytochemical staining as a method of improving diagnostic accuracy in a routine cytopathology laboratory. Cytopathology 1991;2:75-82.","journal-title":"Cytopathology"},{"key":"ref_5","first-page":"137","article-title":"Advances in pneumocystis biology","volume":"2","author":"Matsumoto, Y.; Yoshida, Y.","year":"1986","unstructured":"Matsumoto Y, Yoshida Y. Advances in pneumocystis biology. Parasitol Today 1986;2: 137-42.","journal-title":"Parasitol Today"},{"key":"ref_6","first-page":"1","article-title":"Prospective evaluation of a monoclonal antibody in the diagnosis of Pneumocystis carinii pneumonia","volume":"ii:","author":"Kovacs, J.A.; Gill, V.; Swan, J.C.; Ognibene, F.; Shelhamer, J.; Parrillo, J.E.","year":"1986","unstructured":"Kovacs JA, Gill V, Swan JC, Ognibene F, Shelhamer J, Parrillo JE, et al. Prospective evaluation of a monoclonal antibody in the diagnosis of Pneumocystis carinii pneumonia. Lancet 1986;ii:1-3.","journal-title":"Lancet"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1111\/j.1574-6968.1990.tb03776.x","article-title":"Separation of Pneumocystis carinii from the lung of the steroid-suppressed rat","volume":"70","author":"Taylor, M.B.; Easmon, C.S.F.","year":"1990","unstructured":"Taylor MB, Easmon CSF. Separation of Pneumocystis carinii from the lung of the steroid-suppressed rat. FEMS 1990;70:49-54.","journal-title":"FEMS"},{"key":"ref_8","first-page":"1051","article-title":"Uptake and degradation of Pneumocystis carinii by macrophages in vitro","volume":"118","author":"LA, Von Behren; B, Pesanti","year":"1978","unstructured":"Von Behren LA, Pesanti B. Uptake and degradation of Pneumocystis carinii by macrophages in vitro. Am Rev RespirDis 1978;118:1051-9.","journal-title":"Am Rev RespirDis"},{"key":"ref_9","first-page":"557","article-title":"Fluorescence microscopy of Papanicolaou-stained bronchoalveolar lavage specimens in the diagnosis of Pneumocystis carinii","volume":"33","author":"Pfitzer, P.; Wehle, K.; Blanke, M.; Burrig, K.F.","year":"1989","unstructured":"Pfitzer P, Wehle K, Blanke M, Burrig KF. Fluorescence microscopy of Papanicolaou-stained bronchoalveolar lavage specimens in the diagnosis of Pneumocystis carinii [letter]. Acta Cytologica 1989;33:557-9.","journal-title":"Acta Cytologica"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1016\/0140-6736(92)90469-J","article-title":"Improved diagnosis of Pneumocystis carinii infection by polymerase chain reaction on induced sputum and blood","volume":"340","author":"Lipschik, G.Y.; Gill, V.J.; Lundgren, J.D.; Andrawis, V.A.; Nelson, N.A.; Nielsen, J.O.","year":"1992","unstructured":"Lipschik GY, Gill VJ, Lundgren JD, Andrawis VA, Nelson NA, Nielsen JO, et al. Improved diagnosis of Pneumocystis carinii infection by polymerase chain reaction on induced sputum and blood. Lancet 1992;340:203-6.","journal-title":"Lancet"}],"container-title":["Journal of Clinical Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1136\/jcp.47.12.1108","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,7]],"date-time":"2025-12-07T02:07:11Z","timestamp":1765073231000},"score":1,"resource":{"primary":{"URL":"https:\/\/jcp.bmj.com\/lookup\/doi\/10.1136\/jcp.47.12.1108"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1994,12]]},"references-count":10,"journal-issue":{"issue":"12","published-online":{"date-parts":[[1994,12,1]]},"published-print":{"date-parts":[[1994,12]]}},"alternative-id":["10.1136\/jcp.47.12.1108"],"URL":"https:\/\/doi.org\/10.1136\/jcp.47.12.1108","relation":{},"ISSN":["0021-9746","1472-4146"],"issn-type":[{"value":"0021-9746","type":"print"},{"value":"1472-4146","type":"electronic"}],"subject":[],"published":{"date-parts":[[1994,12]]}}}