{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T21:23:01Z","timestamp":1777929781034,"version":"3.51.4"},"reference-count":14,"publisher":"BMJ","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clin Pathol"],"published-print":{"date-parts":[[1994,6]]},"abstract":"<jats:p>AIMS--To develop a specific test for lupus anticoagulant activity with reduced sensitivity to coagulation factor deficiency that would be suitable for analysis of plasmas from patients receiving oral anticoagulants. METHODS--A coagulation test based on the Taipan snake venom time (TSVT) with a platelet neutralisation procedure (PNP) was developed and compared with dilute Russell's viper venom time (DRVVT). The TSVT was used to test plasmas from patients receiving oral anticoagulant or heparin with mild liver dysfunction and with documented lupus anticoagulant. RESULTS--The optimised conditions for the TSVT were established and a reference range was determined in normal healthy subjects. Results were considered positive for lupus anticoagulant if the ratio was &gt; or = 1.1 and was reduced by &gt; or = 10% or to &lt; 1.1 in the PNP. In 43 samples from patients receiving oral anticoagulants there was no correlation between level of anticoagulation and TSVT, and only seven samples had increased TSVTs. Of these, five corrected on mixing with normal plasma and two gave equivocal results. The patients with mild liver dysfunction all had normal TSVTs. The TSVT in plasmas from patients receiving heparin correlated with the heparin concentrations (as measured by the APTT, r2 = 0.81). Some anticoagulated plasmas showed correction in the PNP and were regarded as false positive. Fourteen of 17 patients known to have lupus anticoagulant (on the basis of DRVVT results) were also positive by the TSVT; two of the remaining three were borderline and one was negative. CONCLUSIONS--The TSVT showed satisfactory intra-assay precision and reasonable sensitivity to lupus anticoagulant, compared with the DRVVT. The TSVT was influenced by the presence of heparin but was not sensitive to the effects of oral anticoagulant. Like other lupus anticoagulant tests, it does not seem to have a 100% detection rate, but this may be due to the presence of lupus anticoagulant subtypes with distinct activities or the requirement of cofactors other than prothrombin or beta 2 glycoprotein-I.<\/jats:p>","DOI":"10.1136\/jcp.47.6.497","type":"journal-article","created":{"date-parts":[[2007,6,9]],"date-time":"2007-06-09T00:50:46Z","timestamp":1181350246000},"page":"497-501","source":"Crossref","is-referenced-by-count":47,"title":["The Taipan snake venom time: a new test for lupus anticoagulant."],"prefix":"10.1136","volume":"47","author":[{"given":"A M","family":"Rooney","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T","family":"McNally","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I J","family":"Mackie","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S J","family":"Machin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[1994,6,1]]},"reference":[{"key":"ref_1","first-page":"263","article-title":"Lupus anticoagulants","volume-title":"Progress in thrombosis and haemostasis. Vol 6","author":"Shapiro, S.S.; Thiagarajan, P.","year":"1982","unstructured":"Shapiro SS, Thiagarajan P. Lupus anticoagulants. In: Spaet TH, ed. Progress in thrombosis and haemostasis. Vol 6. New York: Grune & Stratton, 1982:263-85."},{"key":"ref_2","first-page":"621","article-title":"A haemorrhagic disorder caused by circulating anticoagulant in patients with disseminated lupus erythematosus","volume":"31","author":"Conley, C.L.; Hartman, R.C.","year":"1952","unstructured":"Conley CL, Hartman RC. A haemorrhagic disorder caused by circulating anticoagulant in patients with disseminated lupus erythematosus. Jf Clin Invest 1952; 31:621-62.","journal-title":"Jf Clin Invest"},{"key":"ref_3","first-page":"416","article-title":"Thrombosis in systemic lupus erythematosus despite circulating anticoagulants","volume":"62","author":"Bowie, W.E.J.; Thompson, J.H.; Pascuzzi, C.A.; Owen, G.A.","year":"1963","unstructured":"Bowie WEJ, Thompson JH, Pascuzzi CA, Owen GA. 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Edinburgh: Churchill Livingstone, 1989:382."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1020","DOI":"10.1021\/bi00546a029","article-title":"Characterisation of the prothrombin activator from the venom of Oxyuranus scutellatus scutellatus (Taipan venom)","volume":"19","author":"H., Walker; WG, Owen; CT, Esmon","year":"1980","unstructured":"Walker H., Owen WG, Esmon CT. Characterisation of the prothrombin activator from the venom of Oxyuranus scutellatus scutellatus (Taipan venom). Biochemistry 1980;19: 1020-3.","journal-title":"Biochemistry"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"627","DOI":"10.1055\/s-0038-1648201","article-title":"Inventory of exogenous prothrombin activators for the subcommittee on nomenclature of exogenous haemostatic factors of the scientific and standardisation committee of the International Society on Thrombosis and Haemostasis","volume":"65","author":"Rosing, J.; Tans, G.","year":"1991","unstructured":"Rosing J, Tans G. 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