{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T10:55:33Z","timestamp":1785840933511,"version":"3.56.0"},"reference-count":10,"publisher":"BMJ","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clin Pathol"],"published-print":{"date-parts":[[1994,6]]},"abstract":"<jats:p>AIM--To examine lymphocyte subsets in patients with myelodysplastic syndromes (MDS); and to correlate immunohistological variables with prognosis. METHODS--Bone marrow trephine biopsy specimens from 65 patients with MDS were immunophenotyped using a panel of antibodies. A minimum of 1000 cells from representative areas of marrow sections were counted at light microscopy. The association between immunohistological variables and prognosis was assessed. RESULTS--Compared with normal control marrows (n = 23) no major abnormalities of T cells (CD3), T cell subsets (CD4, CD8, CD25, TCR gamma\/delta) or natural killer cells (CD56, CD57) were seen in the 65 patients. In high risk MDS (RAEB, RAEB-t) 19% of the cases showed increased numbers of B lymphocytes compared with none in the low risk group (RA, RARS) (p &lt; 0.0090). Only percentages of B cells above 3% significantly correlated with poor survival (p = 0.0121 for CD19, p = 0.046 for CD22). CONCLUSIONS--The deviations in T lymphocyte counts seen in peripheral blood and in bone marrow aspirates could not be verified in bone marrow biopsy specimens.<\/jats:p>","DOI":"10.1136\/jcp.47.6.505","type":"journal-article","created":{"date-parts":[[2007,6,9]],"date-time":"2007-06-09T00:50:46Z","timestamp":1181350246000},"page":"505-507","source":"Crossref","is-referenced-by-count":13,"title":["Bone marrow lymphocyte subsets in myelodysplastic syndromes."],"prefix":"10.1136","volume":"47","author":[{"given":"W","family":"Hilbe","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W","family":"Eisterer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C","family":"Schmid","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I","family":"Starz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H","family":"Silly","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C","family":"Duba","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C","family":"Ludescher","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J","family":"Thaler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"239","published-online":{"date-parts":[[1994,6,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1111\/j.1365-2141.1982.tb08475.x","article-title":"Proposals for the classification of the myelodysplastic syndromes","volume":"51","author":"Bennet, J.M.; Catovsky, D.; Daniel, M.T.","year":"1982","unstructured":"Bennet JM, Catovsky D, Daniel MT, et al. Proposals for the classification of the myelodysplastic syndromes. BrJ Haematol 1982;51:189-99.","journal-title":"BrJ Haematol"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1111\/j.1365-2141.1987.tb06142.x","article-title":"Chronic and subacute myelomonocytic leukemia in the adult: a report of 60 cases with special reference to prognostic factors","volume":"65","author":"Fenaux, P.; Jouet, J.P.; Zandecki, M.","year":"1987","unstructured":"Fenaux P, Jouet JP, Zandecki M, et al. Chronic and subacute myelomonocytic leukemia in the adult: a report of 60 cases with special reference to prognostic factors. 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Bone marrow analysis of the myelodysplastic syndromes: Histological and immunohistochemical features related to the evolution of overt leukemia. Virchows Arch (Cell Pathol) 1989;57:47-53.","journal-title":"Virchows Arch (Cell Pathol)"},{"key":"ref_8","article-title":"Natural killer cell activity and preleukemia","author":"Takaku, S.; Takaku, F.","year":"1981","unstructured":"Takaku S, Takaku F. Natural killer cell activity and preleukemia. Lancet 1981;ii: 1178.","journal-title":"Lancet"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1600","DOI":"10.1182\/blood.V67.6.1600.1600","article-title":"Lymphocyte subsets in normal bone marrow","volume":"67","author":"Clark, P.; Normansell, D.E.; Innes, D.J.; Hess, C.E.","year":"1986","unstructured":"Clark P, Normansell DE, Innes DJ, Hess CE. Lymphocyte subsets in normal bone marrow. Blood 1986;67:1600-6.","journal-title":"Blood"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1007\/BF00594196","article-title":"Diagnostic and prognostic value of immunohistological bone marrow examination: results in 212 patients with lymphoproliferative disorders","volume":"54","author":"Thaler, J.; Denz, H.; Gattringer, C.","year":"1987","unstructured":"Thaler J, Denz H, Gattringer C, et al. Diagnostic and prognostic value of immunohistological bone marrow examination: results in 212 patients with lymphoproliferative disorders. 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