{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,14]],"date-time":"2025-12-14T10:49:16Z","timestamp":1765709356291,"version":"3.48.0"},"reference-count":12,"publisher":"BMJ","issue":"11","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clin Pathol"],"published-print":{"date-parts":[[1995,11]]},"abstract":"<jats:p>AIMS--To detect intestinal microsporidiosis in paraffin wax embedded biopsy specimens using a fluorescence technique incorporating optical brighteners. METHODS--Eight HIV infected patients with confirmed intestinal microsporidiosis (six with Enterocytozoon bieneusi, one with Encephalitozoon intestinalis and one with Encephalitozoon cuniculi infection) and 10 without infection were studied. Tissue sections of paraffin wax embedded duodenal biopsy specimens were stained with 1% Uvitex 2B, coded and analysed independently by two investigators. RESULTS--In all eight cases with confirmed intestinal microsporidian infection, spores could be detected easily in tissue sections using the fluorescence technique. Spores or other elements consistent with microsporidiosis were not found in the 10 patients without infection. CONCLUSION--Staining of tissue sections from paraffin wax embedded intestinal biopsy specimens with stains incorporating Uvitex 2B is a rapid and easy technique for the diagnosis of intestinal microsporidiosis.<\/jats:p>","DOI":"10.1136\/jcp.48.11.1009","type":"journal-article","created":{"date-parts":[[2007,6,9]],"date-time":"2007-06-09T00:50:46Z","timestamp":1181350246000},"page":"1009-1010","source":"Crossref","is-referenced-by-count":10,"title":["Tissue diagnosis of intestinal microsporidiosis using a fluorescent stain with Uvitex 2B."],"prefix":"10.1136","volume":"48","author":[{"given":"C","family":"Franzen","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A","family":"M\u00fcller","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B","family":"Salzberger","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G","family":"F\u00e4tkenheuer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S","family":"Eidt","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G","family":"Mahrle","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"V","family":"Diehl","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M","family":"Schrappe","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[1995,11,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"475","DOI":"10.1016\/0046-8177(90)90003-N","article-title":"Intestinal microsporidiosis as a cause of diarrhea in human immunodeficiency virus-infected patients: a report of20 cases","volume":"21","author":"Orenstein, J.M.; Chiang, J.; Steinberg, W.; Smith, P.D.; Rotterdam, H.; Kotler, D.P.","year":"1990","unstructured":"Orenstein JM, Chiang J, Steinberg W, Smith PD, Rotterdam H, Kotler DP. Intestinal microsporidiosis as a cause of diarrhea in human immunodeficiency virus-infected patients: a report of20 cases. Hum Pathol 1990;21:475-81.","journal-title":"Hum Pathol"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"426","DOI":"10.1128\/CMR.7.4.426","article-title":"Human microsporidial infections","volume":"7","author":"Weber, R.; Bryan, R.T.; Schwartz, D.A.; Owen, R.L.","year":"1994","unstructured":"Weber R, Bryan RT, Schwartz DA, Owen RL. Human microsporidial infections. Clin Microbiol Rev 1994;7:426-61.","journal-title":"Clin Microbiol Rev"},{"key":"ref_3","article-title":"Disseminated antibody confirmed Encephalitozoon cuniculi with asymptomatic infection of the gastrointestinal tract in a patient with AIDS","author":"Franzen, C.; Schwartz, D.A.; Visvesvara, G.S.; Muller, A.; Schwenk, A.; Salzberger, B.","year":"1995","unstructured":"Franzen C, Schwartz DA, Visvesvara GS, Muller A, Schwenk A, Salzberger B, et al. Disseminated antibody confirmed Encephalitozoon cuniculi with asymptomatic infection of the gastrointestinal tract in a patient with AIDS. Clin Infect Dis 1995 (in press).","journal-title":"Clin Infect Dis"},{"key":"ref_4","first-page":"1249","article-title":"Tissue diagnosis of intestinal microsporidiosis using the Chromotrope-2R modified trichrome stain","volume":"117","author":"Giang, T.T.; Kotler, D.P.; Garro, M.L.; Orenstein, J.M.","year":"1993","unstructured":"Giang TT, Kotler DP, Garro ML, Orenstein JM. Tissue diagnosis of intestinal microsporidiosis using the Chromotrope-2R modified trichrome stain. Arch Pathol Lab Med 1993;117: 1249-51.","journal-title":"Arch Pathol Lab Med"},{"key":"ref_5","first-page":"540","article-title":"Light microscopic diagnosis of microsporidiosis in patients with AIDS","volume":"89","author":"Kotler, D.P.; Giang, T.T.; Garro, M.L.; Orenstein, J.M.","year":"1994","unstructured":"Kotler DP, Giang TT, Garro ML, Orenstein JM. Light microscopic diagnosis of microsporidiosis in patients with AIDS. Am Jf Gastroenterol 1994;89:540-4.","journal-title":"Am Jf Gastroenterol"},{"key":"ref_6","first-page":"261","article-title":"The Warthin-Starry stain in the diagnosis of small intestinal microsporidiosis in HIV-infected patients","volume":"40","author":"Field, A.S.; Mariott, D.J.; Hing, M.C.","year":"1993","unstructured":"Field AS, Mariott DJ, Hing MC. The Warthin-Starry stain in the diagnosis of small intestinal microsporidiosis in HIV-infected patients. Folia Parasitol (Praha) 1993;40: 261-6.","journal-title":"Folia Parasitol (Praha)"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1007\/BF01715501","article-title":"Intestinal microsporidiosis in patients with acquired immunodeficiency syndrome - report of three more German cases","volume":"22","author":"Franzen, C.; Fatkenheuer, G.; Salzberger, B.; Muller, A.; Mahrle, G.; Diehl, V.","year":"1994","unstructured":"Franzen C, Fatkenheuer G, Salzberger B, Muller A, Mahrle G, Diehl V, et al. Intestinal microsporidiosis in patients with acquired immunodeficiency syndrome - report of three more German cases. Infection 1994;22:417-19.","journal-title":"Infection"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"558","DOI":"10.1136\/jcp.44.7.558","article-title":"Histological diagnosis of intestinal microsporidiosis in patients with AIDS","volume":"44","author":"Peacock, C.S.; Blanshard, C.; Tovey, D.G.; Ellis, D.S.; Gazzard, B.G.","year":"1991","unstructured":"Peacock CS, Blanshard C, Tovey DG, Ellis DS, Gazzard BG. Histological diagnosis of intestinal microsporidiosis in patients with AIDS. 7 Clin Pathol 1991;44:558-63.","journal-title":"7 Clin Pathol"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"694","DOI":"10.1136\/jcp.46.8.694","article-title":"Diagnosis of intestinal and disseminated microsporidial infections in patients with HIV by a new rapid fluorescence technique","volume":"46","author":"van Gool T; F, Snijders; P, Reiss; SchattenkerkJKM, Eeftinck; van den Berg Weerman MA; JFWM, Bartelsman","year":"1993","unstructured":"van Gool T, Snijders F, Reiss P, Eeftinck SchattenkerkJKM, van den Berg Weerman MA, Bartelsman JFWM, et al. Diagnosis of intestinal and disseminated microsporidial infections in patients with HIV by a new rapid fluorescence technique. J Clin Pathol 1993;46:694-9.","journal-title":"J Clin Pathol"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1016\/0035-9203(94)90290-9","article-title":"An improved practical and sensitive technique for the detection ofmicrosporidian spores in stool samples","volume":"88","author":"van Gool T; EU, Canning; J, Dankert","year":"1994","unstructured":"van Gool T, Canning EU, Dankert J. An improved practical and sensitive technique for the detection ofmicrosporidian spores in stool samples. Trans R Soc Trop Med Hyg 1994; 88:189-90.","journal-title":"Trans R Soc Trop Med Hyg"},{"key":"ref_11","first-page":"267","article-title":"Staining of microsporidian spores by optical brighteners with remarks on the use of brighteners for the diagnosis of AIDS associated human microsporidiosis","volume":"40","author":"Vara, J.; Dahbiova, R.; Hollister, W.S.; Canning, E.U.","year":"1993","unstructured":"Vara J, Dahbiova R, Hollister WS, Canning EU. Staining of microsporidian spores by optical brighteners with remarks on the use of brighteners for the diagnosis of AIDS associated human microsporidiosis. Folia Parasitol (Praha) 1993;40:267-72.","journal-title":"Folia Parasitol (Praha)"},{"key":"ref_12","first-page":"273","article-title":"An extremely rapid method for detection of microsporidia in biopsy materials from AIDS patients","volume":"40","author":"Vara, J.; Nohynkova, E.; Machala, L.; Spala, J.","year":"1993","unstructured":"Vara J, Nohynkova E, Machala L, Spala J. An extremely rapid method for detection of microsporidia in biopsy materials from AIDS patients. Folia Parasitol (Praha) 1993;40:273-4.","journal-title":"Folia Parasitol (Praha)"}],"container-title":["Journal of Clinical Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1136\/jcp.48.11.1009","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,14]],"date-time":"2025-12-14T10:44:16Z","timestamp":1765709056000},"score":1,"resource":{"primary":{"URL":"https:\/\/jcp.bmj.com\/lookup\/doi\/10.1136\/jcp.48.11.1009"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1995,11]]},"references-count":12,"journal-issue":{"issue":"11","published-online":{"date-parts":[[1995,11,1]]},"published-print":{"date-parts":[[1995,11]]}},"alternative-id":["10.1136\/jcp.48.11.1009"],"URL":"https:\/\/doi.org\/10.1136\/jcp.48.11.1009","relation":{},"ISSN":["0021-9746","1472-4146"],"issn-type":[{"type":"print","value":"0021-9746"},{"type":"electronic","value":"1472-4146"}],"subject":[],"published":{"date-parts":[[1995,11]]}}}