{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,2]],"date-time":"2026-08-02T14:44:29Z","timestamp":1785681869598,"version":"3.56.0"},"reference-count":7,"publisher":"BMJ","issue":"11","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clin Pathol"],"published-print":{"date-parts":[[1995,11]]},"abstract":"<jats:p>AIM--To establish the role of immunohistochemistry (using a limited panel of antibodies) in detecting minimal involvement by follicular lymphoma in routinely processed bone marrow trephine specimens, which show no obvious morphological (light microscopic) evidence of lymphoma; to determine whether bcl-2 immunostaining in bone marrow distinguishes between benign and malignant infiltrates in a patient with nodal follicular lymphoma. METHODS--Twenty seven consecutively selected paraffin wax embedded, formalin fixed bone marrow trephine specimens were stained with the following antibodies: anti-bcl-2, anti-CD79a, anti-CD3, and kappa and lambda light chains, using the Streptavidin biotin complex technique. RESULTS--Five of the 27 cases, which showed no evidence of involvement by follicular lymphoma on routine stains, showed monotypic B cells on immunohistochemistry. Two of the cases were diffuse, while the remaining three showed mini-aggregates around bony trabeculae. In all five cases the lymphomatous infiltrates were strongly bcl-2 positive. Reactive B lymphoid nodules did not show the same degree of bcl-2 positivity, and negative cells could be discerned within the reactive nodules. CONCLUSIONS--There is merit in studying so-called negative bone marrows immunohistochemically in order to detect minimal involvement by follicular lymphoma. A limited panel of antibodies including anti-bcl-2, anti-CD79a and anti-CD3 is usually adequate to accomplish this. Strongly bcl-2 positive lymphoid aggregates in the bone marrow of patients with nodal follicular lymphoma are indicative of lymphoma.<\/jats:p>","DOI":"10.1136\/jcp.48.11.1035","type":"journal-article","created":{"date-parts":[[2007,6,9]],"date-time":"2007-06-09T00:50:46Z","timestamp":1181350246000},"page":"1035-1038","source":"Crossref","is-referenced-by-count":25,"title":["Immunohistochemistry in apparently normal bone marrow trephine specimens from patients with nodal follicular lymphoma."],"prefix":"10.1136","volume":"48","author":[{"given":"R","family":"Chetty","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G","family":"Echezarreta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"Comley","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K","family":"Gatter","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"239","published-online":{"date-parts":[[1995,11,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1097\/00000478-198902000-00002","article-title":"Immunohistochemistry in bone marrow diagnosis","volume":"13","author":"der Valk P, Van; H, Mullink; PC, Huijgens; TM, Tadema; W, Vos; CJLM, Meijer","year":"1989","unstructured":"Van der Valk P, Mullink H, Huijgens PC, Tadema TM, Vos W, Meijer CJLM. Immunohistochemistry in bone marrow diagnosis. Am Surg Pathol 1989;13:97-106.","journal-title":"Am Surg Pathol"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"686","DOI":"10.1016\/0046-8177(92)90326-X","article-title":"Immunoarchitecture of normal human bone marrow: A study of frozen and fixed tissue sections","volume":"23","author":"Shin, S.S.; Sheibani, K.; Kezirian, J.; Nademanee, A.; Forman, S.J.; Lee, S.K.","year":"1992","unstructured":"Shin SS, Sheibani K, Kezirian J, Nademanee A, Forman SJ, Lee SK, et al. Immunoarchitecture of normal human bone marrow: A study of frozen and fixed tissue sections. Hum Pathol 1992;23:686-94.","journal-title":"Hum Pathol"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1600","DOI":"10.1182\/blood.V67.6.1600.1600","article-title":"Lymphocyte subsets in normal bone marrow","volume":"67","author":"Clark, P.; Normansell, D.E.; Innes, D.J.; Hess, C.E.","year":"1986","unstructured":"Clark P, Normansell DE, Innes DJ, Hess CE. Lymphocyte subsets in normal bone marrow. Blood 1986;67:1600-6.","journal-title":"Blood"},{"key":"ref_4","first-page":"389","article-title":"Lymphoid nodules of bone marrow, normal and abnormal","volume":"13","author":"Rywlin, A.M.; Ortega, R.S.; Dominguez, G.J.","year":"1974","unstructured":"Rywlin AM, Ortega RS, Dominguez GJ. Lymphoid nodules of bone marrow, normal and abnormal. Blood 1974;13: 389-400.","journal-title":"Blood"},{"key":"ref_5","first-page":"1","article-title":"Bone marrow manifestations of malignant lymphoma and lymphoma-like conditions","volume":"1","author":"Brunning, R.D.; McKenna, R.W.","year":"1979","unstructured":"Brunning RD, McKenna RW. Bone marrow manifestations of malignant lymphoma and lymphoma-like conditions. 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Leukemia 1992;6:47-59.","journal-title":"Leukemia"}],"container-title":["Journal of Clinical Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1136\/jcp.48.11.1035","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,14]],"date-time":"2025-12-14T16:23:17Z","timestamp":1765729397000},"score":1,"resource":{"primary":{"URL":"https:\/\/jcp.bmj.com\/lookup\/doi\/10.1136\/jcp.48.11.1035"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1995,11]]},"references-count":7,"journal-issue":{"issue":"11","published-online":{"date-parts":[[1995,11,1]]},"published-print":{"date-parts":[[1995,11]]}},"alternative-id":["10.1136\/jcp.48.11.1035"],"URL":"https:\/\/doi.org\/10.1136\/jcp.48.11.1035","relation":{},"ISSN":["0021-9746","1472-4146"],"issn-type":[{"value":"0021-9746","type":"print"},{"value":"1472-4146","type":"electronic"}],"subject":[],"published":{"date-parts":[[1995,11]]}}}