{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,6]],"date-time":"2025-12-06T08:39:10Z","timestamp":1765010350848,"version":"3.46.0"},"reference-count":8,"publisher":"BMJ","issue":"12","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clin Pathol"],"published-print":{"date-parts":[[1995,12]]},"abstract":"<jats:p>This report describes the characterisation of a polyclonal sheep antiserum against the Ki67 antigen. On western blots, this antiserum recognises a pair of bands of high molecular weight identical with those seen with another polyclonal Ki67 antiserum and the MIB 1 monoclonal antibody. The new antiserum showed nuclear staining of a proportion of cells in paraffin wax embedded tissue sections following antigen retrieval using a microwave oven or pressure cooker. This staining pattern was blocked by incubating the serum with the peptide used as immunogen. The proportion and distribution of immunostained nuclei was identical with that seen with the alternative reagents that recognise the Ki67 antigen. The new reagent stained the same proportion of cells when used over a wide range of dilutions. There was no cross-reactivity with unrelated antigens sometimes detected by the monoclonal antibodies.<\/jats:p>","DOI":"10.1136\/jcp.48.12.1138","type":"journal-article","created":{"date-parts":[[2007,6,9]],"date-time":"2007-06-09T00:50:46Z","timestamp":1181350246000},"page":"1138-1140","source":"Crossref","is-referenced-by-count":5,"title":["Detection of Ki67 antigen by a new sheep polyclonal antiserum."],"prefix":"10.1136","volume":"48","author":[{"given":"G M","family":"Reynolds","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D C","family":"Rowlands","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G P","family":"Mead","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[1995,12,1]]},"reference":[{"key":"ref_1","first-page":"597","article-title":"Little missed markers and Ki-67","volume":"68","author":"Schwarting, R.","year":"1993","unstructured":"Schwarting R. Little missed markers and Ki-67. Lab Invest 1993;68:597-9.","journal-title":"Lab Invest"},{"key":"ref_2","first-page":"867","article-title":"Immunobiochemical and molecular biologic characterisation of the cell proliferation-associated nuclear antigen that is defined by monoclonal antibody Ki67","volume":"138","author":"Gerdes, J.; Li, L.; Schlueter, C.; Duchrow, M.; Wohlenberg, C.; Gerlach, C.","year":"1991","unstructured":"Gerdes J, Li L, Schlueter C, Duchrow M, Wohlenberg C, Gerlach C, et al. Immunobiochemical and molecular biologic characterisation of the cell proliferation-associated nuclear antigen that is defined by monoclonal antibody Ki67. Am J Pathol 1991;138:867-73.","journal-title":"Am J Pathol"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1002\/ijc.2910310104","article-title":"Production of a mouse monoclonal antibody reactive with a human nuclear antigen associated with cell proliferation","volume":"31","author":"Gerdes, J.; Schwab, U.; Lemke, H.; Stein, H.","year":"1983","unstructured":"Gerdes J, Schwab U, Lemke H, Stein H. Production of a mouse monoclonal antibody reactive with a human nuclear antigen associated with cell proliferation. Intr Cancer 1983; 31:13-20.","journal-title":"Intr Cancer"},{"key":"ref_4","first-page":"629","article-title":"New Ki-67-equivalent murine monoclonal antibodies (MIB 1-3) generated against bacterially expressed parts of the Ki-67 cDNA containing three 62 base pair repetitive elements encoding for the Ki-67 epitope","volume":"68","author":"Key, G.; Becker, M.H.; Baron, B.; Duchrow, M.J.; Schluter, C.; Flad, H.-D.","year":"1993","unstructured":"Key G, Becker MH, Baron B, Duchrow MJ, Schluter C, Flad H-D, et al. New Ki-67-equivalent murine monoclonal antibodies (MIB 1-3) generated against bacterially expressed parts of the Ki-67 cDNA containing three 62 base pair repetitive elements encoding for the Ki-67 epitope. Lab Invest 1993;68:629-36.","journal-title":"Lab Invest"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"524","DOI":"10.1136\/jcp.47.6.524","article-title":"Epitope analysis of antibodies recognising the cell proliferation associated nuclear antigen previously defined by the antibody Ki-67 (Ki-67 protein)","volume":"47","author":"Kubbutat, M.; Key, G.; Duchrow, M.; Schluter, C.; Flad, H.-D.; Gerdes, J.","year":"1994","unstructured":"Kubbutat M, Key G, Duchrow M, Schluter C, Flad H-D, Gerdes J. Epitope analysis of antibodies recognising the cell proliferation associated nuclear antigen previously defined by the antibody Ki-67 (Ki-67 protein). Jf Clin Pathol 1994;47:524-8.","journal-title":"Jf Clin Pathol"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1080","DOI":"10.1136\/jcp.46.12.1080","article-title":"New antiserum against Ki-67 antigen suitable for double immunostaining of paraffin wax sections","volume":"46","author":"Key, G.; Larsen Peterson, J.; Becker, M.G.H.; Duchrow, M.; Schluter, C.; Askaa, J.","year":"1993","unstructured":"Key G, Larsen Peterson J, Becker MGH, Duchrow M, Schluter C, Askaa J, et al. New antiserum against Ki-67 antigen suitable for double immunostaining of paraffin wax sections. J Clin Pathol 1993;46:1080-4.","journal-title":"J Clin Pathol"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1719","DOI":"10.1016\/S0021-9258(19)77935-6","article-title":"A novel method for producing anti-peptide antibodies. Production of site-specific antibodies to the T cell antigen receptor beta-chain","volume":"263","author":"Posnett, D.; McGrath, H.; Tam, J.","year":"1988","unstructured":"Posnett D, McGrath H, Tam J. A novel method for producing anti-peptide antibodies. Production of site-specific antibodies to the T cell antigen receptor beta-chain. _7 Biol Chem 1988;263:1719-25.","journal-title":"_7 Biol Chem"},{"key":"ref_8","first-page":"100","article-title":"Stathmin expression is a feature of proliferating cells of most, if not all, cell lineages","volume-title":"Lab Invest","volume":"72","author":"Rowlands, D.C.; Williams, A.; Jones, N.A.; Guest, S.S.; Reynolds, G.M.; Barber, P.C.","year":"1995","unstructured":"Rowlands DC, Williams A, Jones NA, Guest SS, Reynolds GM, Barber PC, et al. Stathmin expression is a feature of proliferating cells of most, if not all, cell lineages. Lab Invest 1995;72:100-13."}],"container-title":["Journal of Clinical Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1136\/jcp.48.12.1138","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,6]],"date-time":"2025-12-06T08:36:51Z","timestamp":1765010211000},"score":1,"resource":{"primary":{"URL":"https:\/\/jcp.bmj.com\/lookup\/doi\/10.1136\/jcp.48.12.1138"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1995,12]]},"references-count":8,"journal-issue":{"issue":"12","published-online":{"date-parts":[[1995,12,1]]},"published-print":{"date-parts":[[1995,12]]}},"alternative-id":["10.1136\/jcp.48.12.1138"],"URL":"https:\/\/doi.org\/10.1136\/jcp.48.12.1138","relation":{},"ISSN":["0021-9746","1472-4146"],"issn-type":[{"type":"print","value":"0021-9746"},{"type":"electronic","value":"1472-4146"}],"subject":[],"published":{"date-parts":[[1995,12]]}}}