{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T12:10:30Z","timestamp":1790511030609,"version":"4.1.0"},"reference-count":10,"publisher":"BMJ","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clin Pathol"],"published-print":{"date-parts":[[1995,3]]},"abstract":"<jats:p>AIMS--To investigate the incidence of functional hyposplenism in a group of patients who had undergone allogeneic bone marrow transplantation (BMT). METHODS--Splenic function was assessed by counting the number of gluteraldehyde fixed red blood cells containing pits or indentations as examined by interference phase microscopy. Normal values are &lt; 2% whereas splenectomy patients have values of 25 to 40%. RESULTS--Twenty eight BMT recipients (17 men, 11 women) were studied at varying periods post-transplant and the results compared with 20 healthy volunteers and 10 patients who had undergone splenectomy or had splenic atrophy because of haematological conditions. Of the 28 BMT recipients, one had undergone a prior splenectomy; of the remaining 27 patients, four (15%) had evidence of functional hyposplenism with between 5.0 and 34.0% pitted cells. Of these four patients, one had active extensive chronic graft versus host disease (GvHD) which has been previously reported to be associated with functional hyposplenism following transplantation. Only one of the four patients had peripheral blood red cell changes typical of hyposplenism. CONCLUSION--These results confirm that extensive chronic GvHD is associated with hyposplenism. Intermediate degrees of functional hyposplenism may also occur following BMT in the absence of chronic GvHD and in the absence of haematological features of hyposplenism on routine blood films. This may be of significance in mediating the susceptibility to infection with encapsulating bacteria seen following allogeneic BMT.<\/jats:p>","DOI":"10.1136\/jcp.48.3.257","type":"journal-article","created":{"date-parts":[[2007,6,9]],"date-time":"2007-06-09T00:50:46Z","timestamp":1181350246000},"page":"257-259","source":"Crossref","is-referenced-by-count":36,"title":["Functional hyposplenism following allogeneic bone marrow transplantation."],"prefix":"10.1136","volume":"48","author":[{"given":"R J","family":"Cuthbert","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A","family":"Iqbal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A","family":"Gates","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P J","family":"Toghill","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N H","family":"Russell","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"239","published-online":{"date-parts":[[1995,3,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1583","DOI":"10.1182\/blood.V75.7.1583.1583","article-title":"Immunoglobulin G subclass deficiency and pneumococcal infection after allogeneic bone marrow transplantation","volume":"75","author":"Sheridan, J.F.; Tutschka, P.J.; Sedmak, D.D.; Copelan, E.A.","year":"1990","unstructured":"Sheridan JF, Tutschka PJ, Sedmak DD, Copelan EA. Immunoglobulin G subclass deficiency and pneumococcal infection after allogeneic bone marrow transplantation. Blood 1990;75:1583-6.","journal-title":"Blood"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"720","DOI":"10.1182\/blood.V53.4.720.720","article-title":"Analysis of late infections in 89 longterm survivors of bone marrow transplantation","volume":"53","author":"Atkinson, K.; Storb, R.; Prentice, R.L.; Weiden, P.L.; Witherspoon, R.P.; Sullivan, K.","year":"1979","unstructured":"Atkinson K, Storb R, Prentice RL, Weiden PL, Witherspoon RP, Sullivan K, et al. Analysis of late infections in 89 longterm survivors of bone marrow transplantation. Blood 1979;53:720-3 1.","journal-title":"Blood"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1182\/blood.V57.2.267.267","article-title":"Chronic graft versus host disease in 52 patients: Adverse natural cause and successful treatment with combination immunosuppression","volume":"57","author":"Sullivan, K.M.; Shulman, H.M.; Storb, R.; Weiden, P.L.; Witherspoon, R.P.; McDonald, G.B.","year":"1981","unstructured":"Sullivan KM, Shulman HM, Storb R, Weiden PL, Witherspoon RP, McDonald GB, et al. Chronic graft versus host disease in 52 patients: Adverse natural cause and successful treatment with combination immunosuppression. Blood 1981;57:267-76.","journal-title":"Blood"},{"key":"ref_4","first-page":"127","article-title":"IgG2 subclass restriction ofantibody to pneumococcal polysaccharides. Clin Exp Immunol","volume":"63","author":"Barrett, D.J.; Ayoub, E.M.","year":"1986","unstructured":"Barrett DJ, Ayoub EM. IgG2 subclass restriction ofantibody to pneumococcal polysaccharides. Clin Exp Immunol 1986; 63:127-34."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"360","DOI":"10.1182\/blood.V58.2.360.360","article-title":"Recovery of antibody production in human allogeneic marrow graft recipients: influence of time post-transplantation, the presence or absence of chronic graft-versus-host disease, and anti-thymocyte globulin treatment","volume":"58","author":"Witherspoon, R.P.; Storb, R.; Ochs, H.D.; Fluomoy, N.; Kopecky, K.J.; Sullivan, K.M.","year":"1981","unstructured":"Witherspoon RP, Storb R, Ochs HD, Fluomoy N, Kopecky KJ, Sullivan KM, et al. Recovery of antibody production in human allogeneic marrow graft recipients: influence of time post-transplantation, the presence or absence of chronic graft-versus-host disease, and anti-thymocyte globulin treatment. Blood 1981;58:360-8.","journal-title":"Blood"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"461","DOI":"10.7326\/0003-4819-109-6-461","article-title":"Functional asplenia after bone marrow transplantation: A late complication related to extensive chronic graft-versus-host disease","volume":"109","author":"Kalhs, P.; Panser, S.; Kletter, K.; Minar, E.; Stain-Kos, M.; Walter, R.","year":"1988","unstructured":"Kalhs P, Panser S, Kletter K, Minar E, Stain-Kos M, Walter R, et al. Functional asplenia after bone marrow transplantation: A late complication related to extensive chronic graft-versus-host disease. Ann Intern Med, 1988; 109:461-4.","journal-title":"Ann Intern Med"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"805","DOI":"10.7326\/0003-4819-113-10-805","article-title":"Functional asplenia after bone marrow transplantation","volume":"113","author":"Kalhs, P.; Kier, P.; Lechner, K.","year":"1990","unstructured":"Kalhs P, Kier P, Lechner K. Functional asplenia after bone marrow transplantation. Ann Intern Med, 1990;113:805-6.","journal-title":"Ann Intern Med"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1042\/cs0600109","article-title":"Simple method of assessing splenic function ofcoeliac disease","volume":"60","author":"Corazza, G.R.; Bullen, A.W.; Hall, R.; Robinson, P.J.; Losowsky, M.S.","year":"1981","unstructured":"Corazza GR, Bullen AW, Hall R, Robinson PJ, Losowsky MS. Simple method of assessing splenic function ofcoeliac disease. Clin Sci 1981;60:109-13.","journal-title":"Clin Sci"},{"key":"ref_9","first-page":"2512","article-title":"Erythrocyte pits and vacuoles as seen with transmission and scanning election microscopy","volume":"173","author":"Schmitzen, B.; Rucknagel, D.L.; Spencer, H.H.; Aikawa, M.","year":"1971","unstructured":"Schmitzen B, Rucknagel DL, Spencer HH, Aikawa M. Erythrocyte pits and vacuoles as seen with transmission and scanning election microscopy. Science 1971;173:2512.","journal-title":"Science"},{"key":"ref_10","first-page":"431","article-title":"Effective prevention of acute graft versus host disease following allogeneic bone marrow transplantation with low leukaemic relapse using methotrexate and therapeutically monitored levels of cyclosporin","volume":"10","author":"Hunter, A.E.; Bessell, E.M.; Russell, N.H.","year":"1992","unstructured":"Hunter AE, Bessell EM, Russell NH. Effective prevention of acute graft versus host disease following allogeneic bone marrow transplantation with low leukaemic relapse using methotrexate and therapeutically monitored levels of cyclosporin. Bone Marrow Transplant 1992;10:431-4.","journal-title":"Bone Marrow Transplant"}],"container-title":["Journal of Clinical Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1136\/jcp.48.3.257","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,14]],"date-time":"2025-12-14T08:23:54Z","timestamp":1765700634000},"score":1,"resource":{"primary":{"URL":"https:\/\/jcp.bmj.com\/lookup\/doi\/10.1136\/jcp.48.3.257"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1995,3]]},"references-count":10,"journal-issue":{"issue":"3","published-online":{"date-parts":[[1995,3,1]]},"published-print":{"date-parts":[[1995,3]]}},"alternative-id":["10.1136\/jcp.48.3.257"],"URL":"https:\/\/doi.org\/10.1136\/jcp.48.3.257","relation":{},"ISSN":["0021-9746","1472-4146"],"issn-type":[{"value":"0021-9746","type":"print"},{"value":"1472-4146","type":"electronic"}],"subject":[],"published":{"date-parts":[[1995,3]]}}}