{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,14]],"date-time":"2025-12-14T16:43:44Z","timestamp":1765730624904,"version":"3.48.0"},"reference-count":23,"publisher":"BMJ","issue":"9","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clin Pathol"],"published-print":{"date-parts":[[1995,9]]},"abstract":"<jats:p>AIM--To investigate the fate of Streptococcus pneumoniae C-polysaccharide antigen in serum in patients with S pneumoniae bacteraemia. METHOD--In vitro dissociation experiments were performed to demonstrate that C-polysaccharide was masked by ligands in normal and acute phase serum. Serum samples from 22 patients with S pneumoniae bacteraemia were treated to dissociate immune complexes and then tested for C-polysaccharide by enzyme linked immunosorbent assay (ELISA). RESULTS--C-polysaccharide antigen was masked in normal and acute phase serum but could be released by EDTA treatment and detected by ELISA. Antigen was found in six patients ranging in concentration from 2.5 to 200 ng\/ml. Patients with detectable antigen were more likely to die than those in whom antigen was not detected. CONCLUSION--This study demonstrates that C-polysaccharide antigen commonly circulates in patients with S pneumoniae bacteraemia but its presence is masked by ligands present in serum.<\/jats:p>","DOI":"10.1136\/jcp.48.9.803","type":"journal-article","created":{"date-parts":[[2007,6,9]],"date-time":"2007-06-09T00:50:46Z","timestamp":1181350246000},"page":"803-806","source":"Crossref","is-referenced-by-count":7,"title":["Detection of C-polysaccharide in serum of patients with Streptococcus pneumoniae bacteraemia."],"prefix":"10.1136","volume":"48","author":[{"given":"S H","family":"Gillespie","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M D","family":"Smith","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A","family":"Dickens","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J G","family":"Raynes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K P","family":"McAdam","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[1995,9,1]]},"reference":[{"key":"ref_1","first-page":"255","article-title":"Hospital study of adult community acquired pneumonia","volume":"ii:","author":"McFarlane, J.T.; Ward, M.J.; Finch, R.G.; McCrae, A.D.","year":"1982","unstructured":"McFarlane JT, Ward MJ, Finch RG, McCrae AD. 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Infect Immun 1977;15:169-74.","journal-title":"Infect Immun"},{"key":"ref_4","first-page":"851","article-title":"Teichoic acid and lipoteichoic acid of Streptococcus pneumoniae possess identical chain structures. A reinvestigation of teichoic acid","volume":"215","author":"Fischer, W.; Behr, T.; Peter-Katalanic, J.; Egge, H.","year":"1993","unstructured":"Fischer W, Behr T, Peter-Katalanic J, Egge H. Teichoic acid and lipoteichoic acid of Streptococcus pneumoniae possess identical chain structures. A reinvestigation of teichoic acid (C polysaccharide). Eur3rBiochem 1993;215: 851-7.","journal-title":"Eur3rBiochem"},{"key":"ref_5","first-page":"2502","article-title":"Activation of C3 via the alternative complement pathway results in the fixation of C3b on the pneumococcal cell wall","volume":"124","author":"Winkelstein, J.A.; Abramovitz, A.S.; Tomasz, A.","year":"1980","unstructured":"Winkelstein JA, Abramovitz AS, Tomasz A. 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Induction of pulmonary inflammation by components of the pneumococcal cell wall. Am Rev Respir Dis 1987;135:869-74.","journal-title":"Am Rev Respir Dis"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"535","DOI":"10.1093\/infdis\/151.3.535","article-title":"The relative role of inflammation in pneumococcal meningitis","volume":"151","year":"1985","unstructured":"Tuomanen E, Tomasz A, Hengstler B, Zak 0. The relative role of inflammation in pneumococcal meningitis. Infect Dis 1985;151:535-40.","journal-title":"Infect Dis"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"806","DOI":"10.1093\/infdis\/163.4.806","article-title":"Differences of pathophysiology in experimental meningitis caused by three strains of Streptococcus pneumoniae","volume":"163","author":"Tauber, M.G.; Burroughs, M.; Neimoller, U.M.; Kusler, H.; Borschber, U.; Tuomanen, E.","year":"1991","unstructured":"Tauber MG, Burroughs M, Neimoller UM, Kusler H, Borschber U, Tuomanen E. Differences of pathophysiology in experimental meningitis caused by three strains of Streptococcus pneumoniae. Infect Dis 1991; 163:806-11.","journal-title":"Infect Dis"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"561","DOI":"10.7326\/0003-4819-84-5-561","article-title":"Antigenemia in fulminant pneumococcemia","volume":"84","author":"Coonrod, J.D.; Leach, R.P.","year":"1976","unstructured":"Coonrod JD, Leach RP. Antigenemia in fulminant pneumococcemia. Ann Intern Med 1976;84:561-3.","journal-title":"Ann Intern Med"},{"issue":"179","key":"ref_11","first-page":"335","article-title":"Pneumococcal C and type polysaccharide detection in the concentrated urine of patients with bacteremia","volume":"1990","author":"Bromberg, K.; Tannis, G.; Rodgers, A.","unstructured":"Bromberg K, Tannis G, Rodgers A. Pneumococcal C and type polysaccharide detection in the concentrated urine of patients with bacteremia. Med Microbiol Immunol (Bert) 1990,179:335-8.","journal-title":"Med Microbiol Immunol (Bert)"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1084","DOI":"10.1177\/22.12.1084","article-title":"Peroxidase-labelled antibody. A new method of conjugation","volume":"22","author":"Nakane, P.K.; Kawaoi, A.","year":"1974","unstructured":"Nakane PK, Kawaoi A. Peroxidase-labelled antibody. A new method of conjugation. J Histochem Cytochem 1974; 22:1084-91.","journal-title":"J Histochem Cytochem"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"749","DOI":"10.1136\/jcp.47.8.749","article-title":"Diagnosis of Streptococcus pneumoniae pneumonia by quantitative enzyme-linked immunosorbent assay of C-polysaccharide antigen","volume":"47","author":"Gillespie, S.H.; Smith, M.D.; Dickens, A.; Raynes, J.G.; McAdam, K.P.W.J.","year":"1994","unstructured":"Gillespie SH, Smith MD, Dickens A, Raynes JG, McAdam KPWJ. Diagnosis of Streptococcus pneumoniae pneumonia by quantitative enzyme-linked immunosorbent assay of C-polysaccharide antigen. J Clin Pathol 1994;47: 749-51.","journal-title":"J Clin Pathol"},{"key":"ref_14","first-page":"236","article-title":"Detection of circulating parasite antigen and specific antibody in experimental Toxocara canis infection. Clin Exp Immunol","volume":"74","author":"Robertson, B.D.; Burkot, T.R.; Gillespie, S.H.; Kennedy, M.W.; Wambai, Z.; Maizels, R.M.","year":"1988","unstructured":"Robertson BD, Burkot TR, Gillespie SH, Kennedy MW, Wambai Z, Maizels RM. Detection of circulating parasite antigen and specific antibody in experimental Toxocara canis infection. Clin Exp Immunol 1988;74:236-41."},{"key":"ref_15","first-page":"249","article-title":"Evidence for the breakdown of pneumococcal","volume-title":"polysaccharide in vivo. Proc Soc Exp Biol Med","volume":"162","author":"Coonrod, J.D.","year":"1979","unstructured":"Coonrod JD. Evidence for the breakdown of pneumococcal polysaccharide in vivo. Proc Soc Exp Biol Med 1979;162: 249-53."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"2193","DOI":"10.4049\/jimmunol.112.6.2193","article-title":"Physical and immunologic properties of pneumococcal capsular polysaccharide produced during human infection","volume":"112","author":"Coonrod, J.D.","year":"1974","unstructured":"Coonrod JD. Physical and immunologic properties of pneumococcal capsular polysaccharide produced during human infection.J Immunol 1974;112:2193-201.","journal-title":"J Immunol"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"759","DOI":"10.7326\/0003-4819-60-5-759","article-title":"Pneumococcal bacteraemia with especial reference to bacteraemic pneumococcal pneumonia","volume":"60","author":"Austrian, R.; Gold, J.","year":"1964","unstructured":"Austrian R, Gold J. Pneumococcal bacteraemia with especial reference to bacteraemic pneumococcal pneumonia. Ann Intern Med 1964;60:759-76.","journal-title":"Ann Intern Med"},{"key":"ref_18","first-page":"263","article-title":"Complement in pneumococcal infections with varying degrees of severity. Scand J7 Infect","volume":"13","author":"Prellner, K.","year":"1981","unstructured":"Prellner K. Complement in pneumococcal infections with varying degrees of severity. Scand J7 Infect Dis 1981;13: 263-8.","journal-title":"Dis"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"3308","DOI":"10.4049\/jimmunol.133.6.3308","article-title":"Blood clearance by anti-phosphorylcholine antibodies as a mechanism of protection in experimental bacteraemia","volume":"133","author":"McDaniel, L.S.; Benjamin, W.H.; Forman, C.; Briles, D.E.","year":"1984","unstructured":"McDaniel LS, Benjamin WH, Forman C, Briles DE. Blood clearance by anti-phosphorylcholine antibodies as a mechanism of protection in experimental bacteraemia. J Immunol 1984;133:3308-12.","journal-title":"J Immunol"},{"key":"ref_20","first-page":"2374","article-title":"Human C-reactive protein is protective against fatal Streptococcus pneumoniae infection in mice","volume":"128","author":"Yother, J.; Volanakis, J.E.; Briles, D.E.","year":"1982","unstructured":"Yother J, Volanakis JE, Briles DE. Human C-reactive protein is protective against fatal Streptococcus pneumoniae infection in mice. Immunol 1982;128:2374-6. _","journal-title":"Immunol"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1128\/IAI.50.1.213-217.1985","article-title":"Correlation ofserum opsonins with in vitro phagocytosis of Streptococcus pneumoniae","volume":"50","author":"Chudwin, D.S.; Astrip, S.G.; Korenblit, A.; Schiffman, G.; Rao, S.","year":"1985","unstructured":"Chudwin DS, Astrip SG, Korenblit A, Schiffman G, Rao S. Correlation ofserum opsonins with in vitro phagocytosis of Streptococcus pneumoniae. 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Commercial latex agglutination tests for detection of Haemophilus influenzae type b and Streptococcus pneumoniae antigens in patients with bacteremic pneumonia. 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