{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T21:33:21Z","timestamp":1792100001998,"version":"4.4.0"},"reference-count":15,"publisher":"BMJ","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clin Pathol"],"published-print":{"date-parts":[[1996,2]]},"abstract":"<jats:p>OBJECTIVE: To investigate the reactivity with TIA-1 and TIA-2, two monoclonal antibodies that recognise, respectively, granular structures in T lymphocytes and the T cell receptor chain in cells from a variety of T cell disorders. METHODS: Cytoplasmic staining with TIA-1 and TIA-2 was carried out by the immunoalkaline phosphatase anti-alkaline phosphatase technique in 67 cases with a T cell disorder: 31 large granular lymphocyte (LGL) leukaemia, nine T-prolymphocytic leukaemia (T-PLL), five Sezary syndrome, four peripheral T cell lymphoma (PTCL), 13 T cell lymphocytosis, and five T-acute lymphoblastic leukaemia (T-ALL). All had over 75% abnormal T cells which were CD2+, CD3+, CD5+, CD7+, and negative with B cell markers. RESULTS: TIA-1 was positive in 77% cases of LGL leukaemia and half of the PTCL and T-ALL, whereas it was negative in all Sezary syndrome and most T-PLL (8\/9) and reactive T-lymphocytosis (10\/13). In LGL leukaemia, TIA-1 was positive irrespective of the membrane phenotype, whether CD8+, CD4- or CD4+, CD8-, and was more often positive in cases where cells were CD16+, CD56+, or CD57+. TIA-2 was positive in 60% of cases encompassing all diagnostic types of T cell disorder. There was no correlation between TIA-2 expression and that of other T cell markers, activation antigens, and natural killer markers. CONCLUSIONS: The pattern of TIA-1 expression in T cell malignancies may help in the differential diagnosis among LGL leukaemia (high expression), T cell lymphocytosis and other T cell diseases (low expression). As TIA-2 is expressed in over 95% mature T lymphocytes and thymic cells, its assessment may be useful to demonstrate aberrant phenotypes which can be exploited for detecting minimal residual disease.<\/jats:p>","DOI":"10.1136\/jcp.49.2.154","type":"journal-article","created":{"date-parts":[[2007,6,9]],"date-time":"2007-06-09T00:50:46Z","timestamp":1181350246000},"page":"154-158","source":"Crossref","is-referenced-by-count":20,"title":["Expression of TIA-1 and TIA-2 in T cell malignancies and T cell lymphocytosis."],"prefix":"10.1136","volume":"49","author":[{"given":"E","family":"Matutes","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E","family":"Coelho","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M J","family":"Aguado","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R","family":"Morilla","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A","family":"Crawford","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K","family":"Owusu-Ankomah","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D","family":"Catovsky","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"239","published-online":{"date-parts":[[1996,2,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"567","DOI":"10.1136\/jcp.42.6.567","article-title":"The French-American-British (FAB) Cooperative Group. Proposals for the classification of chronic B and T lymphoid leukaemias","volume":"42","author":"Bennett, J.M.; Catovsky, D.; Daniel, M.T.; Flandrin, G.; Galton, D.A.G.; Gralnick, H.R.","year":"1989","unstructured":"Bennett JM, Catovsky D, Daniel MT, Flandrin G, Galton DAG, Gralnick HR, et al. The French-American-British (FAB) Cooperative Group. Proposals for the classification of chronic B and T lymphoid leukaemias. Jf Clin Pathol 1989;42:567-84.","journal-title":"Jf Clin Pathol"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"81","DOI":"10.3109\/10428199109068049","article-title":"Mature T-cell leukemias and leukemia\/lymphoma syndromes. Review of our experience in 175 cases","volume":"4","author":"Matutes, E.; Catovsky, D.","year":"1991","unstructured":"Matutes E, Catovsky D. Mature T-cell leukemias and leukemia\/lymphoma syndromes. Review of our experience in 175 cases. Leukemia Lymphoma 1991;4:81-91.","journal-title":"Leukemia Lymphoma"},{"key":"ref_3","first-page":"574","article-title":"A monoclonal antibody reactive with a 15 kDa cytoplasmic granule associated protein defines a subpopulation of CD8 + T-lymphocytes","volume":"144","author":"Anderson, P.; Nagler-Anderson, C.; O'Brien, C.; Levine, H.; Waatkins, S.; Slayter, H.S.","year":"1990","unstructured":"Anderson P, Nagler-Anderson C, O'Brien C, Levine H, Waatkins S, Slayter HS, et al. A monoclonal antibody reactive with a 15 kDa cytoplasmic granule associated protein defines a subpopulation of CD8 + T-lymphocytes. J Immunol 1990;144:574-82.","journal-title":"J Immunol"},{"key":"ref_4","first-page":"1899","article-title":"Monoclonal antibodies reactive with the T cell receptor 4 chain: production and characterization using a new method","volume":"143","author":"Anderson, P.; Blue, M.L.; O'Brien, C.; Schlossman, S.F.","year":"1989","unstructured":"Anderson P, Blue ML, O'Brien C, Schlossman SF. Monoclonal antibodies reactive with the T cell receptor 4 chain: production and characterization using a new method. J Immunol 1989;143: 1899-904.","journal-title":"J Immunol"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1038\/341159a0","article-title":"CD3negative natural killer cells express 4 TCR as part of a novel molecular complex","volume":"341","author":"Anderson, P.; Caliguri, M.; Ritz, J.; Schlossman, S.F.","year":"1989","unstructured":"Anderson P, Caliguri M, Ritz J, Schlossman SF. CD3negative natural killer cells express 4 TCR as part of a novel molecular complex. Nature 1989;341: 159-62","journal-title":"Nature"},{"key":"ref_6","first-page":"2274","article-title":"Fcy receptor type III (CD 1 6) is included in the 4 NK receptor complex expressed by human","volume-title":"natural killer cells. Proc Nad Acad Sci","volume":"87","author":"Anderson, P.; Caligiuri, M.; RikJ, Schlossman; S.F.","year":"1990","unstructured":"Anderson P, Caligiuri M, RikJ, Schlossman SF. Fcy receptor type III (CD 1 6) is included in the 4 NK receptor complex expressed by human natural killer cells. Proc Nad Acad Sci USA 1990;87:2274."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"4263","DOI":"10.4049\/jimmunol.147.12.4263","article-title":"Structural similarity between Fc receptors and T cell receptors. Expression of the y-subunit of FCc in human T cells, natural killer cells and thymocytes","volume":"147","author":"Vivier, E.; Rochet, N.; Kochan, J.P.; Presky, D.H.; Schlossman, S.F.; Anderson, P.","year":"1991","unstructured":"Vivier E, Rochet N, Kochan JP, Presky DH, Schlossman SF, Anderson P. Structural similarity between Fc receptors and T cell receptors. Expression of the y-subunit of FCc in human T cells, natural killer cells and thymocytes. J Immunol 1991;147:4263-70.","journal-title":"J Immunol"},{"key":"ref_8","first-page":"5610","article-title":"Decreased expression of the signal-transducing 4 chains in tumor-infiltrating T-cells and NK cells ofpatients with colorectal carcinoma","volume":"53","author":"Nakagomi, H.; Peterssonn, M.; Magnusson, I.; Juhlia, C.; Matsuda, M.; Mellstedt, H.","year":"1993","unstructured":"Nakagomi H, Peterssonn M, Magnusson I, Juhlia C, Matsuda M, Mellstedt H, et al. Decreased expression of the signal-transducing 4 chains in tumor-infiltrating T-cells and NK cells ofpatients with colorectal carcinoma. Cancer Res 1993;53:5610-2.","journal-title":"Cancer Res"},{"key":"ref_9","first-page":"5613","article-title":"Loss of T-cell receptor 4 chain and p56I\"k in T-cells infiltrating human renal cell carcinoma","volume":"53","author":"Finke, J.H.; Zea, A.H.; Stanley, J.; Longo, D.L.; Mizoguchi, H.; Tubbs, R.R.","year":"1993","unstructured":"Finke JH, Zea AH, Stanley J, Longo DL, Mizoguchi H, Tubbs RR, et al. Loss of T-cell receptor 4 chain and p56I\"k in T-cells infiltrating human renal cell carcinoma. Cancer Res 1993;53:5613-6.","journal-title":"Cancer Res"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"91","DOI":"10.3109\/10428199309148509","article-title":"Cytolytic (TIA-1 +) tumor infiltrating lymphocytes in B cell non-Hodgkin's lymphomas","volume":"9","author":"Diaz, J.; Tubbs, R.; Stoler, M.; Grogan, T.","year":"1993","unstructured":"Diaz J, Tubbs R, Stoler M, Grogan T. Cytolytic (TIA-1 +) tumor infiltrating lymphocytes in B cell non-Hodgkin's lymphomas. Leukemia Lymphoma 1993;9:91-4.","journal-title":"Leukemia Lymphoma"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1093\/ajcp\/88.1.43","article-title":"Immunoalkaline phosphatase labelling of terminal transferase in hematological samples","volume":"88","author":"Erber, W.N.; Mason, D.Y.","year":"1987","unstructured":"Erber WN, Mason DY. Immunoalkaline phosphatase labelling of terminal transferase in hematological samples. Am Clin Pathol 1987;88:43-50. _J","journal-title":"Am Clin Pathol"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1177\/32.2.6198355","article-title":"Immunoenzymatic labelling of monoclonal antibodies using immunocomplexes of alkaline phosphatase and monoclonal anti-alkaline phosphatase (APAAP complexes)","volume":"32","author":"Cordell, J.L.; Falini, B.; Erber, W.N.; Ghosh, W.N.; Abdulaziz, Z.; MacDonald, S.","year":"1984","unstructured":"Cordell JL, Falini B, Erber WN, Ghosh WN, Abdulaziz Z, MacDonald S, et al. Immunoenzymatic labelling of monoclonal antibodies using immunocomplexes of alkaline phosphatase and monoclonal anti-alkaline phosphatase (APAAP complexes). Histochem Cytochem 1984; 32:219-29.","journal-title":"Histochem Cytochem"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"843","DOI":"10.1136\/jcp.47.9.843","article-title":"Demonstration of cytoplasmic and nuclear antigens in acute leukaemia using flow cytometry","volume":"47","author":"Farahat, N.; van der Plas, D.; Praxedes, M.; Morilla, R.; Matutes, E.; Catovsky, D.","year":"1994","unstructured":"Farahat N, van der Plas D, Praxedes M, Morilla R, Matutes E, Catovsky D. Demonstration of cytoplasmic and nuclear antigens in acute leukaemia using flow cytometry. Clin Pathol 1994;47:843-9.","journal-title":"Clin Pathol"},{"key":"ref_14","first-page":"1380","article-title":"T-cells infiltrating renal cell carcinoma display a poor proliferative response even though they can produce interleukin 2 and express interleukin 2 receptors","volume":"53","author":"Alexander, J.P.; Kudoh, S.; Melsop, K.A.; Hamilton, T.A.; Edinger, M.G.; Tubbs, R.R.","year":"1993","unstructured":"Alexander JP, Kudoh S, Melsop KA, Hamilton TA, Edinger MG, Tubbs RR, et al. T-cells infiltrating renal cell carcinoma display a poor proliferative response even though they can produce interleukin 2 and express interleukin 2 receptors. Cancer Res 1993;53:1380-7.","journal-title":"Cancer Res"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1795","DOI":"10.1126\/science.1465616","article-title":"Alterations in signal transduction molecules in T lymphocytes from tumor-bearing mice","volume":"258","author":"Mizoguchi, H.; O'Shea, J.J.; Longo, D.L.; Loeffler, C.M.; McVicar, D.W.; Ochoa, A.C.","year":"1992","unstructured":"Mizoguchi H, O'Shea JJ, Longo DL, Loeffler CM, McVicar DW, Ochoa AC. Alterations in signal transduction molecules in T lymphocytes from tumor-bearing mice. Science 1992;258:1795-8.","journal-title":"Science"}],"container-title":["Journal of Clinical Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1136\/jcp.49.2.154","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,21]],"date-time":"2025-11-21T18:11:07Z","timestamp":1763748667000},"score":1,"resource":{"primary":{"URL":"https:\/\/jcp.bmj.com\/lookup\/doi\/10.1136\/jcp.49.2.154"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1996,2]]},"references-count":15,"journal-issue":{"issue":"2","published-online":{"date-parts":[[1996,2,1]]},"published-print":{"date-parts":[[1996,2]]}},"alternative-id":["10.1136\/jcp.49.2.154"],"URL":"https:\/\/doi.org\/10.1136\/jcp.49.2.154","relation":{},"ISSN":["0021-9746","1472-4146"],"issn-type":[{"value":"0021-9746","type":"print"},{"value":"1472-4146","type":"electronic"}],"subject":[],"published":{"date-parts":[[1996,2]]}}}