{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T07:04:07Z","timestamp":1788246247299,"version":"build-2803163510"},"reference-count":13,"publisher":"BMJ","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clin Pathol"],"published-print":{"date-parts":[[1996,5]]},"abstract":"<jats:p>AIM: To compare the haemoglobin (Hb) H inclusion test with a polymerase chain reaction (PCR) test in routine screening for alpha thalassaemia. METHODS: Ninety nine peripheral blood samples from Chinese patients with mean corpuscular volume below 80 fl were screened for alpha thalassaemia using the HbH inclusion test and by PCR utilising primers bridging the common deletion breakpoint of the South East Asian (--SEA\/) deletion. RESULTS: The HbH inclusion test was positive in 78 (79%) patients, 73 (93.7%) of whom carried the (--SEA\/) deletion on analysis of their DNA by PCR, as did one patient with a negative HbH inclusion test. CONCLUSIONS: These results suggest that in areas with a high prevalence of the (--SEA\/) deletion, such as Hong Kong, the HbH inclusion test can be replaced by PCR as the investigation of choice in screening for alpha thalassaemia.<\/jats:p>","DOI":"10.1136\/jcp.49.5.411","type":"journal-article","created":{"date-parts":[[2007,6,9]],"date-time":"2007-06-09T00:50:46Z","timestamp":1181350246000},"page":"411-413","source":"Crossref","is-referenced-by-count":17,"title":["Comparison of the HbH inclusion test and a PCR test in routine screening for alpha thalassaemia in Hong Kong."],"prefix":"10.1136","volume":"49","author":[{"given":"A Y","family":"Chan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C K","family":"So","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L C","family":"Chan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"239","published-online":{"date-parts":[[1996,5,1]]},"reference":[{"key":"ref_1","first-page":"1081104","volume-title":"A review of the molecular genetics of the human ox-globin gene cluster. Blood","volume":"73","author":"Higgs, D.R.; Vickers, M.A.; Wilkie, A.O.M.; Pretorius, I.M.; Jarman, A.P.; Weatherall, D.J.","year":"1989","unstructured":"Higgs DR, Vickers MA, Wilkie AOM, Pretorius IM, Jarman AP, Weatherall DJ. A review of the molecular genetics of the human ox-globin gene cluster. Blood 1989;73:1081104."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1016\/0009-9120(90)90525-Y","article-title":"Prenatal diagnosis of alpha and beta thalassemias and hemophilia A: Experience in Hong Kong","volume":"23","author":"Chan, V.","year":"1990","unstructured":"Chan V. Prenatal diagnosis of alpha and beta thalassemias and hemophilia A: Experience in Hong Kong. Clin Biochem 1990;23:79-84.","journal-title":"Clin Biochem"},{"key":"ref_3","first-page":"1012","article-title":"Screening for a-thalassemia. 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Blood 1992; 80:517-22.","journal-title":"Blood"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1056\/NEJM198601093140203","article-title":"Embryonic -globin chains in adults: a marker for a-thalassaemia due to a 17-5kb deletion","volume":"314","author":"Chui, D.H.K.; Wong, S.C.; Chung, S.-W.; Patterson, M.; Bhargaua, S.; Poon, M.-C.","year":"1986","unstructured":"Chui DHK, Wong SC, Chung S-W, Patterson M, Bhargaua S, Poon M-C. Embryonic -globin chains in adults: a marker for a-thalassaemia due to a 17-5kb deletion. N Engl J7 Med 1986;314:76-9.","journal-title":"N Engl J7 Med"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"499","DOI":"10.3109\/03630268808991638","article-title":"Different forms of HbH disease in the Chinese","volume":"12","author":"Chan, V.; Chan, T.K.; Todd, D.","year":"1988","unstructured":"Chan V, Chan TK, Todd D. Different forms of HbH disease in the Chinese. 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China: molecular and haematological characterization","volume":"86","author":"Liang, R.; Liang, S.; Jiang, N.H.; Wen, X.J.; Zhao, J.B.; Nechtman, J.F.","year":"1994","unstructured":"Liang R, Liang S, Jiang NH, Wen XJ, Zhao JB, Nechtman JF, et al. ot and f thalassaemia among Chinese children in Guangxi Province, P.R. China: molecular and haematological characterization. Br J Haematol 1994;86:351-4.","journal-title":"Br J Haematol"},{"key":"ref_13","first-page":"861","article-title":"Comparison of HbH inclusion bodies with embryonic 4 globin in the screening for a thalassaemia","volume":"48","author":"Chan, L.C.; So, J.C.C.; Chui, D.H.K.","year":"1995","unstructured":"Chan LC, So JCC, Chui DHK. 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