{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,14]],"date-time":"2025-12-14T10:39:57Z","timestamp":1765708797418,"version":"3.48.0"},"reference-count":12,"publisher":"BMJ","issue":"9","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clin Pathol"],"published-print":{"date-parts":[[1996,9]]},"abstract":"<jats:p>AIM: To study the value of serum holo-transcobalamin II (holo-TCII) measurements in the differential diagnosis of macrocytosis. METHODS: Holo-TCII concentrations were measured in serum samples from 50 healthy non-vegetarian subjects and 30 patients with macrocytosis, using a technique based on the adsorption of holo-TCII with amorphous, precipitated silica. Deoxyuridine (dU) suppression tests were performed on the bone marrow cells of all the patients. Haematological diagnoses were made using standard criteria. RESULTS: The causes of macrocytosis were cobalamin (Cbl) deficiency due to pernicious anaemia or following partial gastrectomy (10 patients), dietary folate deficiency with\/without Cb1 deficiency (four patients), chronic alcoholism (four patients), myelodysplastic syndrome (five patients), treatment with methotrexate or azathioprine (three patients), and congenital dyserythropoietic anaemia (CDA) (four patients). Undetectable or low holo-TCII concentrations were found in all patients with Cb1 deficiency and in some or all patients from each of the other diagnostic categories. There was also no correlation between the dU suppressed value and the holo-TCII concentration: all 15 patients with high dU suppressed values and nine of 15 with normal dU suppressed values, including four patients with CDA, had low holo-TCII concentrations. CONCLUSIONS: Measurements of serum holo-TCII concentrations by the silica adsorption method are not of value in the differential diagnosis of macrocytosis. The finding of low serum holo-TCII concentrations in patients with macrocytosis due to causes other than Cb1 deficiency may result not only from a state of negative Cb1 balance but also from other factors, such as increased utilisation of holo-TCII as a consequence of erythroid hyperplasia.<\/jats:p>","DOI":"10.1136\/jcp.49.9.755","type":"journal-article","created":{"date-parts":[[2007,6,9]],"date-time":"2007-06-09T00:50:46Z","timestamp":1181350246000},"page":"755-758","source":"Crossref","is-referenced-by-count":24,"title":["Limited value of serum holo-transcobalamin II measurements in the differential diagnosis of macrocytosis."],"prefix":"10.1136","volume":"49","author":[{"given":"S N","family":"Wickramasinghe","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I D","family":"Ratnayaka","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[1996,9,1]]},"reference":[{"key":"ref_1","first-page":"47","article-title":"The transport of endogenous vitamin B12 in normal human serum","volume":"51","author":"England, J.M.; Down, M.C.; Wise, I.J.; Linnell, J.C.","year":"1976","unstructured":"England JM, Down MC, Wise IJ, Linnell JC. The transport of endogenous vitamin B12 in normal human serum. Clin Sci Mol Med 1976;51:47-52.","journal-title":"Clin Sci Mol Med"},{"key":"ref_2","first-page":"453","article-title":"The carriers of native vitamin B12 in normal human serum","volume":"53","author":"Hall, C.A.","year":"1977","unstructured":"Hall CA. The carriers of native vitamin B12 in normal human serum. Clin Sci Mol Med 1977;53:453-7.","journal-title":"Clin Sci Mol Med"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"499","DOI":"10.1016\/S0950-3536(05)80218-5","article-title":"Transcobalamin II and the membrane receptor for the transcobalamin II-cobalamin complex","volume":"8","author":"Rothenberg, S.P.; Quadros, E.V.","year":"1995","unstructured":"Rothenberg SP, Quadros EV. Transcobalamin II and the membrane receptor for the transcobalamin II-cobalamin complex. 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A method for performing deoxyuridine suppression tests on microtitre plates. Clin Lab Haematol 1986;8:61-5.","journal-title":"Clin Lab Haematol"},{"key":"ref_8","first-page":"455","article-title":"Determination of vitamin B12 (cobalamin) in serum and erythrocytes by radioassay, and ofholo-transcobalamin II (holo-TCII) and holo-haptocorrin (holo-TCI and III) in serum by adsorbing holo-TCII on microfine silica. J7 Nutr Biochem","volume":"2","author":"Das, K.C.; Manusselis, C.; Herbert, V.","year":"1991","unstructured":"Das KC, Manusselis C, Herbert V. Determination of vitamin B12 (cobalamin) in serum and erythrocytes by radioassay, and ofholo-transcobalamin II (holo-TCII) and holo-haptocorrin (holo-TCI and III) in serum by adsorbing holo-TCII on microfine silica. 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