{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T06:30:07Z","timestamp":1791181807548,"version":"4.1.0"},"reference-count":33,"publisher":"BMJ","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clin Pathol"],"published-print":{"date-parts":[[1997,2]]},"abstract":"<jats:p>AIM: To study cerebrospinal fluid (CSF) concentrations of plasminogen activator inhibitor type-1 (PAI-1) in patients with neurological disease. METHODS: CSF PAI-1 concentrations were measured in 51 patients with neurological disease and 20 reference subjects using an ELISA. The patient group comprised three patients with viral meningitis, 20 with encephalitis, nine with acute lymphoblastic (n = 7) and myeloid (n = 2) leukaemia (with central nervous system involvement), and 19 with multiple sclerosis. RESULTS: Raised PAI-1 concentrations were observed in patients with leukaemia, encephalitis and multiple sclerosis. There was no difference in the mean concentrations of PAI-1 in patients with meningitis when compared with the reference subjects. The highest mean (SEM) PAI-1 concentration was found in patients with leukaemia (1.28 (0.36) ng\/ml), and the next highest in those with encephalitis (1.19 (0.20) ng\/ml). these values were much higher than those in patients with viral meningitis. In a previous report, raised CSF tissue-type plasminogen activator (tPA) activities were detected in patients with multiple sclerosis, leukaemia and encephalitis, with mean activities in decreasing order. PAI-1 concentrations in the same patients were the reverse of their corresponding tPA activities, being higher in those with leukaemia and encephalitis, than in patients with multiple sclerosis. There was no association between CSF PAI-1 concentrations and age in either patients or controls. Similarly, there was no association between CSF PAI-1 concentrations and urokinase-type plasminogen activator (uPA). CONCLUSIONS: Raised CSF PAI-1 concentrations may be used as a non-specific marker of neurological disease. Moreover, PAI-1 may play an important role in regulating the functions tPA, and probably uPA, in CSF.<\/jats:p>","DOI":"10.1136\/jcp.50.2.157","type":"journal-article","created":{"date-parts":[[2007,6,9]],"date-time":"2007-06-09T00:50:46Z","timestamp":1181350246000},"page":"157-160","source":"Crossref","is-referenced-by-count":34,"title":["Cerebrospinal fluid plasminogen activator inhibitor-1 in patients with neurological disease."],"prefix":"10.1136","volume":"50","author":[{"given":"F O","family":"Akenami","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"Koskiniemi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"F\u00e4rkkil\u00e4","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A","family":"Vaheri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"239","published-online":{"date-parts":[[1997,2,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"381","DOI":"10.1182\/blood.V69.2.381.381","article-title":"Plasminogen activator inhibitors","volume":"69","author":"Sprengers, E.D.; Kluft, C.","year":"1987","unstructured":"Sprengers ED, Kluft C. Plasminogen activator inhibitors. Blood 1987;69:381-7.","journal-title":"Blood"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1055\/s-2007-1002762","article-title":"Type 1 plasminogen activator inhibitor and its potential influence on thrombolytic therapy","volume":"14","author":"Loskutoff, D.J.","year":"1988","unstructured":"Loskutoff DJ. Type 1 plasminogen activator inhibitor and its potential influence on thrombolytic therapy. Semin Thromb Hemost 1988;14:100-9.","journal-title":"Semin Thromb Hemost"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1159\/000469153","article-title":"Plasminogen activator inhibitors-a review","volume":"40","author":"Kruithof, E.K.O.","year":"1988","unstructured":"Kruithof EKO. Plasminogen activator inhibitors-a review. Enzyme 1988;40:113-21.","journal-title":"Enzyme"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1016\/S0065-230X(08)60028-7","article-title":"Plasminogen activators, tissue degradation and cancer","volume":"44","author":"Dano, K.; Andreasen, P.A.; Grondahl-Hansen, J.; Kristensen, K.; Nielsen, L.; Skriver, L.","year":"1985","unstructured":"Dano K, Andreasen PA, Grondahl-Hansen J, Kristensen K, Nielsen L, Skriver L. Plasminogen activators, tissue degradation and cancer. Adv Cancer Res 1985;44: 139-266.","journal-title":"Adv Cancer Res"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1016\/S0065-230X(08)61002-7","article-title":"Directed plasminogen activation at the surface of normal and malignant cells","volume":"57","author":"Pollanen, J.; Stephens, R.W.; Vaheri, A.","year":"1991","unstructured":"Pollanen J, Stephens RW, Vaheri A. Directed plasminogen activation at the surface of normal and malignant cells. Adv Cancer Res 1991;57:273-328.","journal-title":"Adv Cancer Res"},{"key":"ref_6","first-page":"1","article-title":"Plasminogen activator inhibitors: hormonally regulated serpins","volume":"88","author":"Andreasen, P.A.; Georg, B.; Lund, L.R.; Riccio, A.; Stacey, S.N.","year":"1990","unstructured":"Andreasen PA, Georg B, Lund LR, Riccio A, Stacey SN. Plasminogen activator inhibitors: hormonally regulated serpins. Mol Cell Endocrinol 1990;88:1-19.","journal-title":"Mol Cell Endocrinol"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"3903","DOI":"10.1073\/pnas.74.9.3903","article-title":"Synthesis of a fibrinolytic activator and inhibitor by endothelial cells","volume":"74","author":"Loskutoff, D.J.; Edgington, T.S.","year":"1977","unstructured":"Loskutoff DJ, Edgington TS. Synthesis of a fibrinolytic activator and inhibitor by endothelial cells. Proc Natl Acad Sci USA 1977;74:3903-7.","journal-title":"Proc Natl Acad Sci USA"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1136\/jcp.44.2.139","article-title":"Distribution of plasminogen activator inhibitor (PAI-1) in tissue","volume":"44","author":"Simpson, A.J.; Booth, N.A.; Moor, N.R.; Benneth, B.","year":"1991","unstructured":"Simpson AJ, Booth NA, Moor NR, Benneth B. Distribution of plasminogen activator inhibitor (PAI-1) in tissue. J Clin Pathol 1991;44: 139-43.","journal-title":"J Clin Pathol"},{"key":"ref_9","first-page":"99","article-title":"Plasminogen activators and inhibitors in gliomas: an immunohistochemical study","volume":"7","author":"Caccamo, D.V.; Keohane, M.E.; McKeever, P.E.","year":"1994","unstructured":"Caccamo DV, Keohane ME, McKeever PE. Plasminogen activators and inhibitors in gliomas: an immunohistochemical study. Mod Pathol 1994;7:99-104.","journal-title":"Mod Pathol"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"256","DOI":"10.1097\/00005072-199405000-00006","article-title":"Immunohistochemical localization of plasminogen activator inhibitor type 1 in human brain tumours","volume":"53","author":"Kono, S.; Rao, J.S.; BrunerJM, Sawaya; R.","year":"1994","unstructured":"Kono S, Rao JS, BrunerJM, Sawaya R. Immunohistochemical localization of plasminogen activator inhibitor type 1 in human brain tumours. 7 Neuropathol Exp Neurol 1994;53: 256-62.","journal-title":"7 Neuropathol Exp Neurol"},{"key":"ref_11","first-page":"3656","article-title":"Expression and localization of urokinasetype plasminogen activator in human astrocytomas in vivo","volume":"54","author":"Yamamoto, M.; Sawaya, R.; Mohanam, S.; Bindal, A.K.; Bruner, J.M.; Oka, K.","year":"1994","unstructured":"Yamamoto M, Sawaya R, Mohanam S, Bindal AK, Bruner JM, Oka K, et al. Expression and localization of urokinasetype plasminogen activator in human astrocytomas in vivo. Cancer Res 1994;54:3656-61.","journal-title":"Cancer Res"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"10396","DOI":"10.1016\/S0021-9258(18)81633-7","article-title":"Regulation of type 1 plasminogen activator inhibitor gene expression in cultured bovine aortic endothelial cells. Induction by transforming growth factor-3, lipopolysaccharide, in tumour necrosis factor-a","volume":"264","author":"Sawey, M.; Podor, T.J.; Loskutoff, D.J.","year":"1989","unstructured":"Sawey M, Podor TJ, Loskutoff DJ. Regulation of type 1 plasminogen activator inhibitor gene expression in cultured bovine aortic endothelial cells. Induction by transforming growth factor-3, lipopolysaccharide, in tumour necrosis factor-a. J7 Biol Chem 1989;264: 10396-401.","journal-title":"J7 Biol Chem"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"17467","DOI":"10.1016\/S0021-9258(18)45403-8","article-title":"Transforming growth factor-, induction of type 1 plasminogen activator inhibitor","volume":"262","year":"1987","unstructured":"Laiho M, Saksela 0, Keski-Oja J. Transforming growth factor-, induction of type 1 plasminogen activator inhibitor. J Biol Chem 1987;262: 17467-74.","journal-title":"J Biol Chem"},{"key":"ref_14","first-page":"181","article-title":"PAI-1 synthesis in the human hepatoma cell line Hep-G is increased by cytokines-evidence that the liver contributes to acute phase behaviour of PAI-1","volume":"65","author":"JP, De Boer; ni, Abbink; MC, Brouwer; C, Meijer; D, Roem; GP, Voorn","year":"1991","unstructured":"De Boer JP, Abbink ni, Brouwer MC, Meijer C, Roem D, Voorn GP, et al. PAI-1 synthesis in the human hepatoma cell line Hep-G is increased by cytokines-evidence that the liver contributes to acute phase behaviour of PAI-1. Thromb Haemost 1991;65:181-5."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"286","DOI":"10.1016\/0165-6147(92)90087-M","article-title":"Cytokines and neuropathology","volume":"13","author":"Morganti-Korsmann, M.C.; Kossman, T.; Wahl, S.M.","year":"1992","unstructured":"Morganti-Korsmann MC, Kossman T, Wahl SM. Cytokines and neuropathology. Trends Pharmacol 1992;13:286-91.","journal-title":"Trends Pharmacol"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"3644","DOI":"10.1016\/S0021-9258(17)43143-7","article-title":"Inactivation of tissue plasminogen activator in plasma. Demonstration of a complex with a new rapid inhibitor","volume":"259","author":"Wiman, B.; Chmielweska, J.; Ranby, M.","year":"1984","unstructured":"Wiman B, Chmielweska J, Ranby M. Inactivation of tissue plasminogen activator in plasma. Demonstration of a complex with a new rapid inhibitor. J Biol Chem 1984;259: 3644-7.","journal-title":"J Biol Chem"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"907","DOI":"10.1182\/blood.V64.4.907.907","article-title":"Demonstration of a fast acting inhibitor of plasminogen activators in human plasma","volume":"64","author":"Kruithof, E.K.O.","year":"1984","unstructured":"Kruithof EKO. Demonstration of a fast acting inhibitor of plasminogen activators in human plasma. Blood 1984;64: 907-13.","journal-title":"Blood"},{"key":"ref_18","first-page":"265","article-title":"The role of the fibrinolytic systems","volume":"105","author":"Wiman, B.","year":"1985","unstructured":"Wiman B. The role of the fibrinolytic systems. J Lab Clin Med 1985;105:265-70.","journal-title":"J Lab Clin Med"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1557","DOI":"10.1056\/NEJM198512193132501","article-title":"Increased plasma levels of rapid inhibitor of tissue plasminogen activator in young survivors of myocardial infarction","volume":"313","author":"Hamsten, A.; Wiman, B.; De Faire, U.; Blomback, M.","year":"1985","unstructured":"Hamsten A, Wiman B, De Faire U, Blomback M. Increased plasma levels of rapid inhibitor of tissue plasminogen activator in young survivors of myocardial infarction. N Engl J Med 1985;313: 1557-63.","journal-title":"N Engl J Med"},{"key":"ref_20","first-page":"313","article-title":"Defective fibrinolysis in blood and vein walls in recurrent \"idiopathic\" venous thrombosis","volume":"138","author":"Isacsson, S.; Nilsson, I.M.","year":"1972","unstructured":"Isacsson S, Nilsson IM. Defective fibrinolysis in blood and vein walls in recurrent \"idiopathic\" venous thrombosis. Acta Chir Scand 1972;138:313-9.","journal-title":"Acta Chir Scand"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"818","DOI":"10.1172\/JCI111777","article-title":"Generation in plasma of a fast-acting inhibitor plasminogen activator in response to endotoxin stimulation","volume":"75","author":"Colucci, M.; Paramo, J.A.; Collen, D.","year":"1985","unstructured":"Colucci M, Paramo JA, Collen D. Generation in plasma of a fast-acting inhibitor plasminogen activator in response to endotoxin stimulation. J Clin Invest 1985;75:818-24.","journal-title":"J Clin Invest"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1055\/s-0038-1661154","article-title":"The fast inhibitor of tissue plasminogen activator during pregnancy","volume":"52","author":"Wiman, B.; Csemiczky, G.; Marsk, L.; Robbe, H.","year":"1984","unstructured":"Wiman B, Csemiczky G, Marsk L, Robbe H. The fast inhibitor of tissue plasminogen activator during pregnancy. Thromb Haemost 1984;52:124-6.","journal-title":"Thromb Haemost"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1097\/00001721-199404000-00002","article-title":"Plasminogen activator inhibitor-I in the cerebrospinal fluid as an index of neurological disease","volume":"5","author":"Sutton, R.; Keohane, M.E.; Van der Berg, SR, Gonias; S.L.","year":"1994","unstructured":"Sutton R, Keohane ME, Van der Berg SR, Gonias SL. Plasminogen activator inhibitor-I in the cerebrospinal fluid as an index of neurological disease. Blood Coagul Fibrinolysis 1994;5:167-71.","journal-title":"Blood Coagul Fibrinolysis"},{"issue":"381 -7","key":"ref_24","article-title":"Plasminogen activator inhibitor-I in the pathogenesis of delayed radiation damage in rat spinal cord in vivo","volume":"81","author":"Sawaya, R.; Rayford, A.; Kono, S.; Ang, K.K.; Feng, Y.; Stephens, C.L.","year":"1994","unstructured":"Sawaya R, Rayford A, Kono S, Ang KK, Feng Y, Stephens CL, et al. Plasminogen activator inhibitor-I in the pathogenesis of delayed radiation damage in rat spinal cord in vivo. Neurosurg 1994;81:381 -7.","journal-title":"Neurosurg"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1016\/0303-8467(94)90119-8","article-title":"Different types of plasminogen activator activity in human brain tumours: relation with peritumoral oedema?","volume":"96","author":"Gijtenbeek, J.M.; Emeis, J.J.; Van-der-Sande, J.J.; Lulf, R.E.","year":"1994","unstructured":"Gijtenbeek JM, Emeis JJ, Van-der-Sande JJ, Lulf RE. Different types of plasminogen activator activity in human brain tumours: relation with peritumoral oedema? Clin Neurol Neurosurg 1994;96:305-9.","journal-title":"Clin Neurol Neurosurg"},{"key":"ref_26","first-page":"59","article-title":"Components of the plasminogen activation system in uveal melanoma-a clinicopathological study","volume":"175","author":"TJ, De Vries; CM, Mooy; MR, Van Balken; GP, Luyten; PH, Quax; HW, Verspaget","year":"1995","unstructured":"De Vries TJ, Mooy CM, Van Balken MR, Luyten GP, Quax PH, Verspaget HW, et al. Components of the plasminogen activation system in uveal melanoma-a clinicopathological study. _7 Pathol 1995;175:59-67."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"577","DOI":"10.1136\/jcp.49.7.577","article-title":"Cerebrospinal fluid activity of tissue plasminogen activator in patients with neurological diseases","volume":"49","author":"Akenami, F.O.T.; Siren, V.; Koskiniemi, M.; Siimes, M.A.; Teravainen, H.; Vaheri, A.","year":"1996","unstructured":"Akenami FOT, Siren V, Koskiniemi M, Siimes MA, Teravainen H, Vaheri A. Cerebrospinal fluid activity of tissue plasminogen activator in patients with neurological diseases. Clin Pathol 1996;49:577-80.","journal-title":"Clin Pathol"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"917","DOI":"10.1055\/s-0038-1651028","article-title":"Blood collection in strong acidic citrate anticoagulant used in a study of dietary influence on basal tPA activity","volume":"62","author":"Ranby, M.; Sundell, I.B.; Nilsson, T.K.","year":"1989","unstructured":"Ranby M, Sundell IB, Nilsson TK. Blood collection in strong acidic citrate anticoagulant used in a study of dietary influence on basal tPA activity. Thromb Haemost 1989;62: 917-22.","journal-title":"Thromb Haemost"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1645","DOI":"10.1182\/blood.V70.5.1645.1645","article-title":"Plasminogen activator inhibitor 1: development of radioimmunoassay and observations on its plasma concentration during venous occlusion and after platelet aggregation","volume":"70","author":"Kruithof, K.O.; Nicolosa, G.; Bachmann, F.","year":"1987","unstructured":"Kruithof KO, Nicolosa G, Bachmann F. Plasminogen activator inhibitor 1: development of radioimmunoassay and observations on its plasma concentration during venous occlusion and after platelet aggregation. Blood 1987;70:1645-53.","journal-title":"Blood"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"608","DOI":"10.1093\/clinids\/6.5.608","article-title":"Cerebrospinal fluid alterations in herpes simplex virus encephalitis","volume":"6","author":"Koskiniemi, M.; Vaheri, A.; Taskinen, E.","year":"1984","unstructured":"Koskiniemi M, Vaheri A, Taskinen E. Cerebrospinal fluid alterations in herpes simplex virus encephalitis. Rev Infect Dis 1984;6:608-17.","journal-title":"Rev Infect Dis"},{"key":"ref_31","first-page":"340","article-title":"Festoff BW Plasminogen activator inhibitor 1, the primary regulator of fibrinolysis, in the normal human cerebrospinal fluid..7 Neurosci","volume":"34","author":"Rao, J.S.; Chen, M.","year":"1993","unstructured":"Rao JS, Chen M, Festoff BW Plasminogen activator inhibitor 1, the primary regulator of fibrinolysis, in the normal human cerebrospinal fluid..7 Neurosci Res 1993;34:340-5.","journal-title":"Res"},{"key":"ref_32","first-page":"1260","article-title":"Interleukin 1 and lipopolysaccharide induce an inhibitor of tissue type plasminogen activator in vivo and in cultured endothelial cells. _7 Exp Med","volume":"163","author":"Emeis, J.J.; Kooistra, T.","year":"1986","unstructured":"Emeis JJ, Kooistra T. Interleukin 1 and lipopolysaccharide induce an inhibitor of tissue type plasminogen activator in vivo and in cultured endothelial cells. _7 Exp Med 1986;163: 1260-6."},{"key":"ref_33","first-page":"166","article-title":"Plasminogen activators and their inhibitors in leukaemic cell homogenates","volume":"42","author":"Wada, H.; Kumeda, Y.; Ogasawara, Z.; Ohiwa, M.; Kaneko, T.; Tamaki, S.","year":"1993","unstructured":"Wada H, Kumeda Y, Ogasawara Z, Ohiwa M, Kaneko T, Tamaki S, et al. Plasminogen activators and their inhibitors in leukaemic cell homogenates. Am I Henmatol 1993;42: 166-70.","journal-title":"Am I Henmatol"}],"container-title":["Journal of Clinical Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1136\/jcp.50.2.157","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,13]],"date-time":"2025-12-13T03:57:56Z","timestamp":1765598276000},"score":1,"resource":{"primary":{"URL":"https:\/\/jcp.bmj.com\/lookup\/doi\/10.1136\/jcp.50.2.157"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1997,2]]},"references-count":33,"journal-issue":{"issue":"2","published-online":{"date-parts":[[1997,2,1]]},"published-print":{"date-parts":[[1997,2]]}},"alternative-id":["10.1136\/jcp.50.2.157"],"URL":"https:\/\/doi.org\/10.1136\/jcp.50.2.157","relation":{},"ISSN":["0021-9746","1472-4146"],"issn-type":[{"value":"0021-9746","type":"print"},{"value":"1472-4146","type":"electronic"}],"subject":[],"published":{"date-parts":[[1997,2]]}}}