{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,6]],"date-time":"2025-12-06T05:15:07Z","timestamp":1764998107945,"version":"3.46.0"},"reference-count":23,"publisher":"BMJ","issue":"9","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clin Pathol"],"published-print":{"date-parts":[[1997,9]]},"abstract":"<jats:p>AIMS: To compare the application of a non-radioactive in situ hybridisation (ISH) technique with an immunocytochemical technique for the detection of human parvovirus B19 in formalin fixed, paraffin wax embedded sections of macerated fetal tissue. METHODS: Archived samples of liver, lung or kidney from 19 human fetuses were investigated for parvovirus B19 using a full length digoxigenin labelled DNA probe of 5.5 kb; bound probe was detected using an anti-digoxigenin (alkaline phosphatase) conjugate and visualised using NBT\/BCIP. Immunocytochemical detection of parvovirus B19 was performed using a monoclonal mouse antiparvovirus B19 antiserum, with a streptavidin-biotin complex (horse radish peroxidase) method. Cases were selected to provide a range of diagnostic certainty and a range of degrees of macerative degeneration. RESULTS: Parvovirus B19 was found in 15 of 19 cases using the B19 ISH technique compared with 8 of 19 cases using the immunocytochemical technique. The four negative cases were all controls known to be parvovirus B19 free. All ISH positive cases showed excellent staining with low background regardless of extent of maceration and tissue type. In comparison, sections stained by the immunocytochemical method showed considerable non-specific immunoreactivity in many cases, particularly with severe maceration. Kidney and lung tissues gave the cleanest results. CONCLUSIONS: ISH is more effective than the immunocytochemical technique for the detection of human parvovirus B19 in macerated fetal tissue. The lack of detectable background staining with the ISH technique led to easier interpretation suggesting that this technique should be the method of choice for the investigation of parvovirus B19 in macerated postmortem tissues.<\/jats:p>","DOI":"10.1136\/jcp.50.9.749","type":"journal-article","created":{"date-parts":[[2007,6,9]],"date-time":"2007-06-09T00:50:46Z","timestamp":1181350246000},"page":"749-754","source":"Crossref","is-referenced-by-count":9,"title":["Detection of parvovirus B19 in macerated fetal tissue using in situ hybridisation."],"prefix":"10.1136","volume":"50","author":[{"given":"C","family":"Walters","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D G","family":"Powe","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C J","family":"Padfield","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D G","family":"Fagan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[1997,9,1]]},"reference":[{"key":"ref_1","first-page":"491","article-title":"Virus taxonomy update. Taxonomic decisions ratified at the plenary meeting of the ICTV at the 9th international congress of virology held in Glasgow on the","volume":"133","author":"Pringle, C.R.","year":"1993","unstructured":"Pringle CR. Virus taxonomy update. Taxonomic decisions ratified at the plenary meeting of the ICTV at the 9th international congress of virology held in Glasgow on the 10th August 1993. Arch Virol 1993;133:491-5.","journal-title":"Arch Virol"},{"key":"ref_2","first-page":"72","article-title":"Parvovirus like particles in human sera","volume":"i:","author":"Cossart, Y.E.; Field, A.M.; Cant, B.; Widdows, D.","year":"1975","unstructured":"Cossart YE, Field AM, Cant B, Widdows D. Parvovirus like particles in human sera. Lancet 1975;i:72-3.","journal-title":"Lancet"},{"key":"ref_3","first-page":"25","article-title":"Characterization of the genome of the agent of erythrocyte aplasia permits its classification as a human Parvovirus","volume":"64","author":"Summers, J.; Jones, S.E.; Anderson, M.J.","year":"1983","unstructured":"Summers J, Jones SE, Anderson MJ. Characterization of the genome of the agent of erythrocyte aplasia permits its classification as a human Parvovirus. J Gen Virol 1983;64: 25-7.","journal-title":"J Gen Virol"},{"key":"ref_4","first-page":"59172","article-title":"Haematolgic and haematopoietic consequences of B19 Parvovirus infection","volume":"25","author":"Young, N.","year":"1988","unstructured":"Young N. Haematolgic and haematopoietic consequences of B19 Parvovirus infection. Semin Hemoto 1988;25:59172.","journal-title":"Semin Hemoto"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1237","DOI":"10.1016\/0140-6736(93)91145-C","article-title":"Human parvovirus infection in homozygous sickle-cell disease","volume":"341","author":"Serjeant, G.R.; Serjeant, B.E.; Thomas, P.W.; Anderson, M.J.; Patou, G.; Pattison, JR","year":"1993","unstructured":"Serjeant GR, Serjeant BE, Thomas PW, Anderson MJ, Patou G, Pattison JR. Human parvovirus infection in homozygous sickle-cell disease. Lancet 1993;341:1237-40.","journal-title":"Lancet"},{"key":"ref_6","first-page":"1378","article-title":"Human parvovirus the cause of erythema infectiosum (fifth disease)","volume":"i:","author":"Anderson, M.J.; Jones, S.E.; Fisher-Hoch, S.P.","year":"1983","unstructured":"Anderson MJ, Jones SE, Fisher-Hoch SP. Human parvovirus the cause of erythema infectiosum (fifth disease) [letter]. Lancet 1983;i:1378.","journal-title":"Lancet"},{"key":"ref_7","first-page":"4","article-title":"Parvovirus infection of the human fetus and newborn","volume":"9","author":"Berry, P.J.; Gray, E.S.; Porter, H.J.; Burton, P.A.","year":"1992","unstructured":"Berry PJ, Gray ES, Porter HJ, Burton PA,. Parvovirus infection of the human fetus and newborn. Semin Diagn Pathol 1992;9:4-12.","journal-title":"Semin Diagn Pathol"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1093\/infdis\/156.1.17","article-title":"Human parvovirus during pregnancy","volume":"156","author":"Woerlne, C.H.; Anderson, L.J.; Tattersall, P.","year":"1987","unstructured":"Woerlne CH, Anderson LJ, Tattersall P. Human parvovirus during pregnancy. J Infect Dis 1987; 156: 17-20.","journal-title":"J Infect Dis"},{"key":"ref_9","first-page":"1166","article-title":"Prospective study of human parvovirus (B19) infection in pregnancy","volume":"300","author":"Hall, S.M.; Cohen, B.J.; Mortimer, P.P.; Anderson, M.J.; Pattison, JR, Shirley; J.A.","year":"1990","unstructured":"Hall SM, Cohen BJ, Mortimer PP, Anderson MJ, Pattison JR, Shirley JA, et al. Prospective study of human parvovirus (B19) infection in pregnancy. BM3 1990;300: 1166-70."},{"key":"ref_10","first-page":"214","article-title":"Identification of human remains using DNA isolated from bones stored under various conditions and for different post mortem times","volume":"37","author":"Kubat, M.; Gusic, S.; Martinovic, S.; Zecevic, D.","year":"1996","unstructured":"Kubat M, Gusic S, Martinovic S, Zecevic D. Identification of human remains using DNA isolated from bones stored under various conditions and for different post mortem times. Croatian Medical Journal 1996;37:214-16.","journal-title":"Croatian Medical Journal"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"673","DOI":"10.1136\/jcp.45.8.673","article-title":"Nonisotopic in-situ hybridisation and immunophenotyping of infected cells in the investigation of human fetal Parvovirus infection","volume":"45","author":"Morey, A.L.; Porter, H.J.; Keeling, J.W.; Fleming, K.A.","year":"1992","unstructured":"Morey AL, Porter HJ, Keeling JW, Fleming KA. Nonisotopic in-situ hybridisation and immunophenotyping of infected cells in the investigation of human fetal Parvovirus infection. Clin Pathol 1992;45:673-8.","journal-title":"Clin Pathol"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"577","DOI":"10.1177\/29.4.6166661","article-title":"Use of avidin-biotinperoxidase complex (ABC) in immunoperoxidase techniques; a comparison between ABC and unlabelled antibody (PAP) procedure","volume":"29","author":"Hsu, S.M.; Raine, L.; Fanger, H.","year":"1981","unstructured":"Hsu SM, Raine L, Fanger H. Use of avidin-biotinperoxidase complex (ABC) in immunoperoxidase techniques; a comparison between ABC and unlabelled antibody (PAP) procedure. J Histochem Cytochem 1981;29: 577-80.","journal-title":"J Histochem Cytochem"},{"key":"ref_13","first-page":"1033","article-title":"International parvovirus infection associated with hydrops fetalis","volume":"1984","author":"Brown, T.; Anand, A.; Richie, L.D.; Clewley, J.P.; Ried, T.M.S.","unstructured":"Brown T, Anand A, Richie LD, Clewley JP, Ried TMS. International parvovirus infection associated with hydrops fetalis. Lancet 1984-ii:1033-4.","journal-title":"Lancet"},{"key":"ref_14","first-page":"81","article-title":"Centres for disease control. Risk associated with human parvovirus B19 infection","volume":"38","year":"1989","unstructured":"Centres for disease control. Risk associated with human parvovirus B19 infection. MMWR Morb Mortal Wkly Rep 1989;38:81-97.","journal-title":"MMWR Morb Mortal Wkly Rep"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1016\/S0095-5108(18)30712-7","article-title":"Human parvovirus B19 and pregnancy","volume":"15","author":"Anderson, L.J.; Hurwitz, E.S.","year":"1988","unstructured":"Anderson LJ, Hurwitz ES. Human parvovirus B19 and pregnancy. Clin Perinatal 1988;15:273-86.","journal-title":"Clin Perinatal"},{"key":"ref_16","first-page":"575","article-title":"Estimating the time of death in stillborn fetuses: I. Histologic evaluation of fetal organs; an autopsy study of 150 stillborns","volume":"80","author":"Genest, D.R.; Williams, M.A.; Greene, M.F.","year":"1992","unstructured":"Genest DR, Williams MA, Greene MF. Estimating the time of death in stillborn fetuses: I. Histologic evaluation of fetal organs; an autopsy study of 150 stillborns. Obstet Gynecol 1992;80:575-84.","journal-title":"Obstet Gynecol"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1056\/NEJM198707303170506","article-title":"Chronic bone marrow failure due to persistent B 19 parvovirus infection","volume":"317","author":"Kertzman, G.J.; Ozawa, K.; Cohen, B.","year":"1987","unstructured":"Kertzman GJ, Ozawa K, Cohen B. Chronic bone marrow failure due to persistent B 19 parvovirus infection. N Engl _J Med 1987;317:287-92.","journal-title":"N Engl _J Med"},{"key":"ref_18","first-page":"535","article-title":"Fleming KA","volume":"i:","author":"Porter, H.J.; Quantrill, A.M.","year":"1988","unstructured":"Porter HJ, Quantrill AM, Fleming KA. B 19 Parvovirus infection of myocardial cells [letter]. Lancet 1988;i:535-6.","journal-title":"Parvovirus infection of myocardial cells; Lancet"},{"key":"ref_19","first-page":"566","article-title":"Clinical and histopathological features of parvovirus B 19 infection in the human fetus","volume":"99","author":"Morey, A.L.; Keeling, J.W.; Porter, H.J.; Fleming, K.A.","year":"1992","unstructured":"Morey AL, Keeling JW, Porter HJ, Fleming KA. Clinical and histopathological features of parvovirus B 19 infection in the human fetus. _7 Obstet Gynaecol 1992;99: 566-74."},{"article-title":"Immunohistological detection of human parvovirus B 19 in formalin-fixed, paraffin-embedded tissues","author":"Morey, A.L.; O'Neill, H.J.; Coyle, P.V.; Fleming, K.A.","key":"ref_20","unstructured":"Morey AL, O'Neill HJ, Coyle PV, Fleming KA. Immunohistological detection of human parvovirus B 19 in formalin-fixed, paraffin-embedded tissues. I Pathol 1992;-"},{"key":"ref_21","first-page":"1196204","article-title":"Suppression of endogenous avidin\/ biotin activity in tissues and its relevance to biotin avidin detection systems","volume":"29","author":"Wood, G.S.; Warnke, R.","year":"1981","unstructured":"Wood GS, Warnke R. Suppression of endogenous avidin\/ biotin activity in tissues and its relevance to biotin avidin detection systems. _7 Histochem Cytochem 1981;29:1196204."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1017\/S0022172400060095","article-title":"Diagnostic assays with monoclonal antibodies for human serum parvovirus-like virus (SPLV)","volume":"91","author":"Cohen, B.J.; Mortimer, P.P.; Pereira, M.S.","year":"1983","unstructured":"Cohen BJ, Mortimer PP, Pereira MS. Diagnostic assays with monoclonal antibodies for human serum parvovirus-like virus (SPLV). Journal of Hygiene 1983;91: 113-30.","journal-title":"Journal of Hygiene"},{"key":"ref_23","series-title":"Obstet Gynecol","first-page":"37","article-title":"Identification of human parvovirus B 19 infection in idiopathic nonimmune hydrops fetalis","volume":"174","author":"Jordan, J.A.","year":"1996","unstructured":"Jordan JA. Identification of human parvovirus B 19 infection in idiopathic nonimmune hydrops fetalis. Am71 T Obstet Gynecol 1996;174:37-42."}],"container-title":["Journal of Clinical Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1136\/jcp.50.9.749","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,6]],"date-time":"2025-12-06T05:10:52Z","timestamp":1764997852000},"score":1,"resource":{"primary":{"URL":"https:\/\/jcp.bmj.com\/lookup\/doi\/10.1136\/jcp.50.9.749"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1997,9]]},"references-count":23,"journal-issue":{"issue":"9","published-online":{"date-parts":[[1997,9,1]]},"published-print":{"date-parts":[[1997,9]]}},"alternative-id":["10.1136\/jcp.50.9.749"],"URL":"https:\/\/doi.org\/10.1136\/jcp.50.9.749","relation":{},"ISSN":["0021-9746","1472-4146"],"issn-type":[{"type":"print","value":"0021-9746"},{"type":"electronic","value":"1472-4146"}],"subject":[],"published":{"date-parts":[[1997,9]]}}}