{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T09:26:16Z","timestamp":1789377976434,"version":"build-2803163510"},"reference-count":22,"publisher":"BMJ","issue":"10","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clin Pathol"],"published-print":{"date-parts":[[1998,10]]},"abstract":"<jats:p>AIM: To test a new assay for the detection of human papillomavirus (HPV) DNA, hybrid capture II (HC II), compared with the previous commercialized hybrid capture I (HC I) and polymerase chain reaction (PCR) results on cervical scrapes from fresh cone excision biopsy samples. METHODS: The three methods were used on cervical scrapes from 42 fresh cone excision biopsy samples. There were nine metaplastic and inflammatory lesions, five low grade lesions, and 28 high grade lesions. PCR was performed using the general primers GP5+\/GP6+. The viral load of high risk HPV DNA was estimated by the ratio of relative light units to positive control values in the samples. RESULTS: The sensitivity of HC I for the detection of high grade lesions was 71.4%, while it was 92.8% for HC II and 96.4% for the PCR. Considering only the absence of detectable cervical in situ neoplasia, the specificity was 88.9% for HC I, 66.7% for HC II, and 66.7% for PCR. With HC II, for a ratio of cervical sample to normal control of &gt; 200, the sensitivity for the detection of high grade lesion was only 34.6% with a specificity of 66.7%. CONCLUSIONS: HPV detection with the HC II assay is more sensitive than the previous HC I and represents a more convenient and easier test than PCR for routine use. Nevertheless the viral load estimated with this test cannot be a reliable predictive indicator of high grade lesions.<\/jats:p>","DOI":"10.1136\/jcp.51.10.737","type":"journal-article","created":{"date-parts":[[2008,12,31]],"date-time":"2008-12-31T19:05:02Z","timestamp":1230750302000},"page":"737-740","source":"Crossref","is-referenced-by-count":94,"title":["Hybrid capture II, a new sensitive test for human papillomavirus detection. Comparison with hybrid capture I and PCR results in cervical lesions."],"prefix":"10.1136","volume":"51","author":[{"given":"C","family":"Clavel","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"Masure","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I","family":"Putaud","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K","family":"Thomas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J P","family":"Bory","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R","family":"Gabriel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C","family":"Quereux","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P","family":"Birembaut","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"239","published-online":{"date-parts":[[1998,10,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"796","DOI":"10.1093\/jnci\/87.11.796","article-title":"et al and International Biological Study in Cervical Cancer Study Group. Prevalence of human papillomavirus in cervical cancer: a worldwide perspective","volume":"87","author":"Bosch, F.X.; Manos, M.M.; Munoz, N.","year":"1995","unstructured":"Bosch FX, Manos MM, Munoz N, et al and International Biological Study in Cervical Cancer Study Group. Prevalence of human papillomavirus in cervical cancer: a worldwide perspective. J Natl Cancer Inst 1995;87:796\u2013 802.","journal-title":"J Natl Cancer Inst"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"328","DOI":"10.1097\/00006250-199203000-00002","article-title":"Human papillomavirus infection of the cervix: relative risk association of 15 common anogenital types","volume":"79","author":"Lorincz, A.T.; Reid, R.; Jenson, A.B.","year":"1992","unstructured":"Lorincz AT, Reid R, Jenson AB, et al. Human papillomavirus infection of the cervix: relative risk association of 15 common anogenital types. Obstet Gynecol 1992;79:328\u201337.","journal-title":"Obstet Gynecol"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"404","DOI":"10.1002\/ijc.2910480317","article-title":"DiVerence in prevalence of human papillomavirus genotypes in cytomorphologically normal smears is associated with a history of cervical intraepithelial neoplasia","volume":"48","author":"den Brule AJC, Van; AJM, Walboomers; M, Du Maine","year":"1991","unstructured":"Van den Brule AJC, Walboomers AJM, Du Maine M, et al. DiVerence in prevalence of human papillomavirus genotypes in cytomorphologically normal smears is associated with a history of cervical intraepithelial neoplasia. Int J Cancer 1991;48:404\u20138.","journal-title":"Int J Cancer"},{"key":"ref_4","first-page":"131","article-title":"Molecular pathogenesis of cancer of the cervix and its causation by specific human papillomavirus types","volume":"186","author":"H, Zur Hausen","year":"1994","unstructured":"Zur Hausen H. Molecular pathogenesis of cancer of the cervix and its causation by specific human papillomavirus types. Curr Top Microbiol Immunol 1994;186:131\u201356."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1272","DOI":"10.1056\/NEJM199210293271804","article-title":"A cohort study of the risk of cervical intraepithelial neoplasia grade 2 or 3 in relation to papillomavirus infection","volume":"327","author":"Koutsky, L.A.; Holmes, K.K.; Critchlow, C.W.","year":"1992","unstructured":"Koutsky LA, Holmes KK, Critchlow CW, et al. A cohort study of the risk of cervical intraepithelial neoplasia grade 2 or 3 in relation to papillomavirus infection. N Engl J Med 1992;327:1272\u20138.","journal-title":"N Engl J Med"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1046\/j.1525-1438.1994.04020073.x","article-title":"Human papillomavirus DNA genotypes: prognostic factors for progression of cervical intraepithelial neoplasia","volume":"4","author":"Gaarenstrom, K.N.; Melkert, P.; Walboomers, J.M.M.","year":"1994","unstructured":"Gaarenstrom KN, Melkert P, Walboomers JMM, et al. Human papillomavirus DNA genotypes: prognostic factors for progression of cervical intraepithelial neoplasia. Int J Gynecol Cancer 1994;4:73\u20138.","journal-title":"Int J Gynecol Cancer"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"2975","DOI":"10.1001\/jama.270.24.2975","article-title":"Human papillomavirus DNA testing in the management of cervical neoplasia","volume":"270","author":"Cole, H.M.","year":"1993","unstructured":"Cole HM. Human papillomavirus DNA testing in the management of cervical neoplasia. JAMA 1993;270:2975\u201381.","journal-title":"JAMA"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"946","DOI":"10.1016\/0002-9378(95)90026-8","article-title":"Human papillomavirus testing by hybrid capture appears to be useful in triaging women with a cytologic diagnosis of atypical squamous cells of undetermined significance","volume":"172","author":"Cox, J.T.; Lorincz, A.T.; SchiVman, M.H.","year":"1995","unstructured":"Cox JT, Lorincz AT, SchiVman MH, et al. Human papillomavirus testing by hybrid capture appears to be useful in triaging women with a cytologic diagnosis of atypical squamous cells of undetermined significance. Am J Obstet Gynecol 1995;172:946\u201354.","journal-title":"Am J Obstet Gynecol"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1413","DOI":"10.1002\/cncr.2820710406","article-title":"Controversies in the management of low grade cervical intraepithelial neoplasia","volume":"71","author":"Richart, R.M.; Wright, T.C.","year":"1993","unstructured":"Richart RM, Wright TC. Controversies in the management of low grade cervical intraepithelial neoplasia. Cancer 1993; 71:1413\u201321.","journal-title":"Cancer"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"791","DOI":"10.1128\/JCM.35.3.791-795.1997","article-title":"A general primer GP5+\/GP6+ mediated PCR-enzyme immunoassay method for rapid detection of 14 high-risk and 6 low-risk human papillomavirus genotypes in cervical scrapings","volume":"35","author":"Jacobs, M.V.; Snidjers, P.J.F.; Van den Brule, A.J.C.","year":"1997","unstructured":"Jacobs MV, Snidjers PJF, Van den Brule AJC, et al. A general primer GP5+\/GP6+ mediated PCR-enzyme immunoassay method for rapid detection of 14 high-risk and 6 low-risk human papillomavirus genotypes in cervical scrapings. J Clin Microbiol 1997;35:791\u20135.","journal-title":"J Clin Microbiol"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1016\/0029-7844(94)00399-X","article-title":"A polymerase chain reaction\u2013enzyme linked immunosorbent assay method detecting human papillomavirus in cervical carcinomas and high-grade cervical cancer precursors","volume":"85","author":"Lungu, O.; Sun, X.W.; Wright, T.C.","year":"1995","unstructured":"Lungu O, Sun XW, Wright TC, et al. A polymerase chain reaction\u2013enzyme linked immunosorbent assay method detecting human papillomavirus in cervical carcinomas and high-grade cervical cancer precursors. Obstet Gynecol 1995;85:337\u201342.","journal-title":"Obstet Gynecol"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1136\/jcp.51.1.38","article-title":"DNA-EIA to detect high and low risk HPV genotypes in cervical lesions with E6\/E7 primer mediated multiplex PCR","volume":"51","author":"Clavel, C.; Rihet, S.; Masure, M.","year":"1998","unstructured":"Clavel C, Rihet S, Masure M, et al. DNA-EIA to detect high and low risk HPV genotypes in cervical lesions with E6\/E7 primer mediated multiplex PCR. J Clin Pathol 1998;51:38\u2013 43.","journal-title":"J Clin Pathol"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"649","DOI":"10.1136\/jcp.47.7.649","article-title":"Human papillomavirus detection by hybrid capture and its possible clinical use","volume":"47","author":"Farthing, A.; P., Masterson; Mason, W.P.","year":"1994","unstructured":"Farthing A, Masterson P., Mason WP, et al. Human papillomavirus detection by hybrid capture and its possible clinical use. J Clin Pathol 1994;47:649\u201352.","journal-title":"J Clin Pathol"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"545","DOI":"10.1128\/JCM.33.3.545-550.1995","article-title":"Accuracy and interlaboratory reliability of human papillomavirus DNA testing by hybrid capture","volume":"33","author":"SchiVman, M.A.; Kiviat, N.B.; Burk, R.D.","year":"1995","unstructured":"SchiVman MA, Kiviat NB, Burk RD, et al. Accuracy and interlaboratory reliability of human papillomavirus DNA testing by hybrid capture. J Clin Microbiol 1995;33:545\u201350.","journal-title":"J Clin Microbiol"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1432","DOI":"10.1016\/0002-9378(95)90629-0","article-title":"Evaluation of the hybrid capture human papillomavirus deoxyribonucleic acid detection test","volume":"173","author":"Sun, X.W.; Ferenczy, A.; Johnson, D.","year":"1995","unstructured":"Sun XW, Ferenczy A, Johnson D, et al. Evaluation of the hybrid capture human papillomavirus deoxyribonucleic acid detection test. Am J Obstet Gynecol 1995;173:1432\u20137.","journal-title":"Am J Obstet Gynecol"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1150","DOI":"10.1016\/0002-9378(95)91473-0","article-title":"An evaluation of human papillomavirus testing for intermediateand high-risk types as triage before colposcopy","volume":"172","author":"Hatch, K.D.; Scheider, A.; Addel-Nour, M.W.","year":"1995","unstructured":"Hatch KD, Scheider A, Addel-Nour MW. An evaluation of human papillomavirus testing for intermediateand high-risk types as triage before colposcopy. Am J Obstet Gynecol 1995;172:1150\u20137.","journal-title":"Am J Obstet Gynecol"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"210","DOI":"10.1002\/(SICI)1096-9071(199602)48:2<210::AID-JMV15>3.0.CO;2-I","article-title":"Human papillomavirus detection by hybrid capture in paired cervicovaginal lavage and cervical biopsy specimens","volume":"48","author":"Brown, D.R.; Rawlings, K.; Handy, V.","year":"1996","unstructured":"Brown DR, Rawlings K, Handy V, et al. Human papillomavirus detection by hybrid capture in paired cervicovaginal lavage and cervical biopsy specimens. J Med Virol 1996;48: 210\u20134.","journal-title":"J Med Virol"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"525","DOI":"10.1002\/(SICI)1097-0215(19980209)75:4<525::AID-IJC6>3.0.CO;2-V","article-title":"Comparative analysis of the human papillomavirus detection by hybrid capture assay and routine cytologic screening to detect high grade cervical lesions","volume":"75","author":"Clavel, C.; Bory, J.P.; Rihet, S.","year":"1998","unstructured":"Clavel C, Bory JP, Rihet S, et al. Comparative analysis of the human papillomavirus detection by hybrid capture assay and routine cytologic screening to detect high grade cervical lesions. Int J Cancer 1998;75:525\u20138.","journal-title":"Int J Cancer"},{"key":"ref_19","first-page":"1","article-title":"Hybrid capture method for detection of human papillomavirus DNA in clinical specimens","volume":"7","author":"Lorincz, A.T.","year":"1996","unstructured":"Lorincz AT. Hybrid capture method for detection of human papillomavirus DNA in clinical specimens. Papillomavirus Rep 1996;7:1\u20135.","journal-title":"Papillomavirus Rep"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1016\/0732-8893(95)00187-5","article-title":"Human papillomavirus detection in high-grade squamous intraepithelial lesions. Comparison of hybrid capture assay with a polymerase chain reaction system","volume":"23","author":"Nindl, I.; Zahm, D.M.; Meijer, C.J.L.M.","year":"1995","unstructured":"Nindl I, Zahm DM, Meijer CJLM, et al. Human papillomavirus detection in high-grade squamous intraepithelial lesions. Comparison of hybrid capture assay with a polymerase chain reaction system. Diagn Microbiol Infect Dis 1995;23:161\u20134.","journal-title":"Diagn Microbiol Infect Dis"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1038\/bjc.1994.28","article-title":"Human papillomavirus DNA in abnormal smears as a predictor of high-grade cervical intraepithelial neoplasia","volume":"69","author":"Cuzick, J.; Terry, G.; Ho, L.","year":"1994","unstructured":"Cuzick J, Terry G, Ho L, et al. Human papillomavirus DNA in abnormal smears as a predictor of high-grade cervical intraepithelial neoplasia. Br J Cancer 1994;69: 167\u201371.","journal-title":"Br J Cancer"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1365","DOI":"10.1093\/jnci\/87.18.1365","article-title":"Persistent genital human papillomavirus infection as a risk factor for persistent cervical dysplasia","volume":"87","author":"Ho, G.Y.F.; Burk, R.D.; Klein, S.","year":"1995","unstructured":"Ho GYF, Burk RD, Klein S, et al. Persistent genital human papillomavirus infection as a risk factor for persistent cervical dysplasia. J Natl Cancer Inst 1995;87:1365\u201371.","journal-title":"J Natl Cancer Inst"}],"container-title":["Journal of Clinical Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1136\/jcp.51.10.737","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,14]],"date-time":"2025-12-14T15:28:19Z","timestamp":1765726099000},"score":1,"resource":{"primary":{"URL":"https:\/\/jcp.bmj.com\/lookup\/doi\/10.1136\/jcp.51.10.737"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1998,10]]},"references-count":22,"journal-issue":{"issue":"10","published-online":{"date-parts":[[1998,10,1]]},"published-print":{"date-parts":[[1998,10]]}},"alternative-id":["10.1136\/jcp.51.10.737"],"URL":"https:\/\/doi.org\/10.1136\/jcp.51.10.737","relation":{},"ISSN":["0021-9746","1472-4146"],"issn-type":[{"value":"0021-9746","type":"print"},{"value":"1472-4146","type":"electronic"}],"subject":[],"published":{"date-parts":[[1998,10]]}}}