{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,7]],"date-time":"2025-12-07T03:38:45Z","timestamp":1765078725752,"version":"3.46.0"},"reference-count":17,"publisher":"BMJ","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clin Pathol"],"published-print":{"date-parts":[[1998,4]]},"abstract":"<jats:p>AIM: To compare experience of positive blood cultures in successive years before and after changing from Signal (Unipath) to Bactec 9240 (Becton Dickinson) blood culture systems. METHODS: Analysis of data collected prospectively on 7967 Signal and 7062 Bactec blood culture sets. RESULTS: Significant growths occurred in 5.7% of Signal and 8.9% of Bactec cultures; 33.0% more significant isolates and 24.0% more episodes of bacteraemia were detected in the second year, following introduction of the Bactec system. Inpatient hospital activity increased by 8.2% between the first and second years, although the numbers of blood cultures received actually fell by 11.4%. There were striking increases in numbers of isolates of coagulase negative staphylococci (47.7%) and Enterobacteriaceae (56.8%) from Bactec cultures. Two anaerobic bacteraemias were detected in Signal blood cultures, whereas none was detected by the Bactec system, despite 12.1% of sets including an anaerobic bottle. Of significant positive cultures, 90.2% were detected within one day with the Bactec 9240, compared with only 50.0% of Signal cultures; 20.7% of significant positive Signal blood cultures were detected only on terminal subculture. Microorganisms that were not significant were isolated from 5.1% Signal and 3.8% Bactec cultures. CONCLUSIONS: Compared with the Signal system, the Bactec 9240 offers markedly more rapid and sensitive detection of bacteraemia, together with a lower rate of non-significant isolates. However, using a single PEDS PLUS\/F bottle the few episodes of anaerobic bacteraemia that occur in children are likely to be missed.<\/jats:p>","DOI":"10.1136\/jcp.51.4.302","type":"journal-article","created":{"date-parts":[[2008,12,31]],"date-time":"2008-12-31T19:05:02Z","timestamp":1230750302000},"page":"302-305","source":"Crossref","is-referenced-by-count":5,"title":["Experience of changing between signal and Bactec 9240 blood culture systems in a children's hospital."],"prefix":"10.1136","volume":"51","author":[{"given":"J","family":"Gray","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M","family":"Brockwell","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I","family":"Das","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[1998,4,1]]},"reference":[{"key":"ref_1","first-page":"552","article-title":"Multicenter clinical evaluation of a continuous monitoring blood culture system using fluorescent-sensor technology (BACTEC","volume":"31","author":"Nolte, F.S.; Williams, J.M.; Jerris, R.C.","year":"9240","unstructured":"Nolte FS, Williams JM, Jerris RC, et al. 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