{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T11:04:57Z","timestamp":1777460697264,"version":"3.51.4"},"reference-count":18,"publisher":"BMJ","issue":"11","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clin Pathol"],"published-print":{"date-parts":[[1999,11]]},"abstract":"<jats:p>AIM: To determine the interobserver variation in scoring presence and grade of vulvar intraepithelial neoplasia (VIN) in haematoxylin\/eosin (H\/E) slides, MIB 1 slides, and the combined use of H\/E and MIB 1 slides. METHODS: 10 slides were stained with H\/E and MIB 1 with each of the following diagnoses: normal vulvar skin, VIN 1, VIN 2, and VIN 3. Six observers first scored the H\/E slides separately from the MIB 1 slides and second the combined H\/E and MIB 1 slides. RESULTS: Unweighted group kappa for MIB 1 was 0.62 and the weighted group kappa was 0.91. This was significantly better than the unweighted group kappa for H\/E slides (0.47, p = 0.023) as well as the weighted group kappa for H\/E slides (0.82, p = 0.014). There was no improvement by the combined use of H\/E and MIB 1 slides. VIN 2 is far less confused with VIN 3 in the combined use of H\/E and MIB 1 slides (9%) than in H\/E slides (38%) (p = 0.007). There is a tendency to grade VIN in a two tailed grading system rather than a three tailed grading system, which became more apparent with the combined use of H\/E and MIB 1 slides. CONCLUSIONS: The interobserver variation with sole use of MIB 1 is better than with the use of H\/E stain in VIN. The use of MIB 1 in grading VIN diminishes confusion between VIN 2 and VIN 3 fourfold. A two tailed grading system for VIN seems already to work in daily practice.<\/jats:p>","DOI":"10.1136\/jcp.52.11.820","type":"journal-article","created":{"date-parts":[[2009,1,1]],"date-time":"2009-01-01T00:05:02Z","timestamp":1230768302000},"page":"820-824","source":"Crossref","is-referenced-by-count":25,"title":["The contribution of MIB 1 in the accurate grading of vulvar intraepithelial neoplasia."],"prefix":"10.1136","volume":"52","author":[{"given":"M","family":"van Beurden","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A J","family":"de Craen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H C","family":"de Vet","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J L","family":"Blaauwgeers","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P","family":"Drillenburg","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M P","family":"Gallee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N W","family":"de Kraker","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F B","family":"Lammes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F J","family":"ten Kate","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[1999,11,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1046\/j.1525-1438.1998.09733.x","article-title":"Restricted surgical management of vulvar intraepithelial neoplasia 3: focus on exclusion of invasion and on relief of symptoms","volume":"8","author":"van Beurden M; van der Vange N; ten Kate FJW","year":"1998","unstructured":"van Beurden M, van der Vange N, ten Kate FJW, et al. 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