{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,25]],"date-time":"2025-09-25T13:48:39Z","timestamp":1758808119303,"version":"3.44.0"},"reference-count":16,"publisher":"BMJ","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clin Pathol"],"published-print":{"date-parts":[[1999,6]]},"abstract":"<jats:p>AIM: To develop and evaluate a one day in-house polymerase chain reaction (PCR) assay for the detection of Neisseria gonorrhoeae DNA in urogenital samples. METHODS: 429 urogenital specimens were tested for the presence of N gonorrhoeae by in-house PCR and by Gen-Probe. The PCR assay amplifies target sequences within the N gonorrhoeae cppB gene on the 4.2 kb cryptic plasmid, after which amplicons are detected by a streptavidinbiotin based enzyme immunoassay using an internal probe. Discordant specimens were further evaluated by repeating the PCR and the Gen-Probe assay, and by an additional PCR using another set of 16S primers followed by radioactive detection of amplicons on a Southern blot. RESULTS: Of the 429 samples tested, 15 were found positive by in-house PCR, eight of which were confirmed by Gen-Probe. Of the seven discrepant samples, five were confirmed by 16S PCR and are also considered true positive. The remaining two samples were positive in the in-house PCR only, and are considered false positive. After resolution of discrepant samples, the sensitivities of the N gonorrhoeae assays were 100% and 61.5% for the in-house PCR and Gen-Probe, respectively, while specificities were comparable at 99.5% and 100%. CONCLUSIONS: The in-house PCR for the detection of N gonorrhoeae DNA is at least comparable to Gen-Probe in performance. An extended evaluation period should elucidate if the additional five GO-PCR positive specimens, confirmed by 16S PCR, are caused by persistence of DNA or whether the method is indeed more sensitive.<\/jats:p>","DOI":"10.1136\/jcp.52.6.411","type":"journal-article","created":{"date-parts":[[2008,12,31]],"date-time":"2008-12-31T19:05:02Z","timestamp":1230750302000},"page":"411-414","source":"Crossref","is-referenced-by-count":3,"title":["Evaluation of an in-house polymerase chain reaction for detection of Neisseria gonorrhoeae in urogenital samples."],"prefix":"10.1136","volume":"52","author":[{"given":"R","family":"Roymans","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G","family":"Onland","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A","family":"Jansz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W","family":"Quint","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E","family":"Boel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[1999,6,1]]},"reference":[{"key":"ref_1","first-page":"149","article-title":"Gonococcal infections in the adult. In","volume-title":"Sexually transmitted diseases","author":"Hook, E.W.; Handsfield, H.H.","year":"1990","unstructured":"Hook EW, Handsfield HH. Gonococcal infections in the adult. In: Holmes K, M\u00e5rdh P, Sparling P, eds. Sexually transmitted diseases. New York: McGraw-Hill, 1990:149\u201365."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1128\/JCM.33.2.455-457.1995","article-title":"evaluation of the ligase chain reaction for use with urine for identification of Neisseria gonorrhoeae in females attending a sexually transmitted disease clinic","volume":"33","author":"Smith, K.R.; Ching, S.; Lee, H.","year":"1995","unstructured":"Smith KR, Ching S, Lee H, et al. evaluation of the ligase chain reaction for use with urine for identification of Neisseria gonorrhoeae in females attending a sexually transmitted disease clinic. J Clin Microbiol 1995;33:455\u20137.","journal-title":"J Clin Microbiol"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"3111","DOI":"10.1128\/JCM.33.12.3111-3114.1995","article-title":"Ligase chain reaction for detection of Neisseria gonorrhoeae in urogenital swabs","volume":"33","author":"Ching, S.; Lee, H.; Hook, E.","year":"1995","unstructured":"Ching S, Lee H, Hook E, et al. Ligase chain reaction for detection of Neisseria gonorrhoeae in urogenital swabs. J Clin Microbiol 1995;33:3111\u201314.","journal-title":"J Clin Microbiol"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1142","DOI":"10.1136\/jcp.38.10.1142","article-title":"Evaluation of a direct immunofluorescence test for diagnosis gonorrhea","volume":"38","author":"Ison, C.A.; McClean, K.; Gedney, J.","year":"1985","unstructured":"Ison CA, McClean K, Gedney J. Evaluation of a direct immunofluorescence test for diagnosis gonorrhea. J Clin Pathol 1985;38:1142\u20135.","journal-title":"J Clin Pathol"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1128\/JCM.19.1.57-59.1984","article-title":"Enzyme immunoassay for diagnosis of gonorrhea","volume":"19","author":"Schachter, J.; McCormack, W.M.; Smith, R.F.","year":"1984","unstructured":"Schachter J, McCormack WM, Smith RF, et al. Enzyme immunoassay for diagnosis of gonorrhea. J Clin Microbiol 1984;19:57\u20139.","journal-title":"J Clin Microbiol"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1162","DOI":"10.1128\/JCM.30.5.1162-1166.1992","article-title":"Evaluation of culture and the GenProbe PACE assay for detection of Neisseria gonorrhoeae and Chlamydia trachomatis in endocervical specimens transported to a state health laboratory","volume":"30","author":"Limberger, R.J.; Biega, R.; Evancole, A.","year":"1992","unstructured":"Limberger RJ, Biega R, Evancole A, et al. Evaluation of culture and the GenProbe PACE assay for detection of Neisseria gonorrhoeae and Chlamydia trachomatis in endocervical specimens transported to a state health laboratory. J Clin Microbiol 1992;30:1162\u20136.","journal-title":"J Clin Microbiol"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"2685","DOI":"10.1128\/JCM.29.12.2685-2689.1991","article-title":"Performance of a nonisotopic DNA probe for detection of Chlamydia trachomatis in urogenital specimens","volume":"29","author":"Kluytmans, J.A.J.; Niesters, H.G.M.; Mouton, J.W.","year":"1991","unstructured":"Kluytmans JAJ, Niesters HGM, Mouton JW, et al. Performance of a nonisotopic DNA probe for detection of Chlamydia trachomatis in urogenital specimens. J Clin Microbiol 1991;29:2685\u20139.","journal-title":"J Clin Microbiol"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1128\/JCM.31.1.107-110.1993","article-title":"Value of a DNA probe assay (Gen\u2013Probe) compared with that of culture for diagnosis of gonococcal infection","volume":"31","author":"Vlaspolder, F.; Mutsaers, J.A.E.M.; Blog, F.","year":"1993","unstructured":"Vlaspolder F, Mutsaers JAEM, Blog F, et al. Value of a DNA probe assay (Gen\u2013Probe) compared with that of culture for diagnosis of gonococcal infection. J Clin Microbiol 1993;31: 107\u201310.","journal-title":"J Clin Microbiol"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1136\/jcp.45.5.439","article-title":"Polymerase chain reaction for the detection of Neisseria gonorrhoeae in clinical samples","volume":"45","author":"Ho, B.S.W.; Feng, W.G.; Wong, B.K.C.","year":"1992","unstructured":"Ho BSW, Feng WG, Wong BKC, et al. Polymerase chain reaction for the detection of Neisseria gonorrhoeae in clinical samples. J Clin Pathol 1992;45:439\u201342.","journal-title":"J Clin Pathol"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"581","DOI":"10.1136\/jcp.49.7.581","article-title":"One-day detection of PCR amplified Chlamydia trachomatis DNA in clinical samples: ELISA versus Southern blot hybridisation","volume":"49","author":"Roymans, R.T.J.M.; Onland, G.; Postma, B.H.","year":"1996","unstructured":"Roymans RTJM, Onland G, Postma BH. One-day detection of PCR amplified Chlamydia trachomatis DNA in clinical samples: ELISA versus Southern blot hybridisation. J Clin Pathol 1996;49:581\u20133.","journal-title":"J Clin Pathol"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1002\/jmv.1890370109","article-title":"Infections of the cervix uteri with human papillomavirus and Chlamydia trachomatis","volume":"37","author":"Claas, H.C.J.; Melchers, W.J.G.; Niesters, H.G.M.","year":"1992","unstructured":"Claas HCJ, Melchers WJG, Niesters HGM, et al. Infections of the cervix uteri with human papillomavirus and Chlamydia trachomatis. J Med Virol 1992;37:54\u20137.","journal-title":"J Med Virol"},{"issue":"3","key":"ref_12","first-page":"53","article-title":"Centres of Disease Control. Chlamydia trachomatis infections: policy guidelines for prevention and control","volume":"34","year":"1985","unstructured":"Centres of Disease Control. Chlamydia trachomatis infections: policy guidelines for prevention and control. MMWR 1985;34(suppl 3):53\u201374.","journal-title":"MMWR"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"495","DOI":"10.1128\/JCM.28.3.495-503.1990","article-title":"Rapid and simple method for purification of nucleic acid","volume":"28","author":"Boom, R.; Sol, C.J.A.; Salisman, M.M.M.","year":"1990","unstructured":"Boom R, Sol CJA, Salisman MMM, et al. Rapid and simple method for purification of nucleic acid. J Clin Microbiol 1990;28:495\u2013503.","journal-title":"J Clin Microbiol"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"430","DOI":"10.1128\/JB.163.2.430-438.1985","article-title":"Cryptic plasmid of Neisseria gonorrhoeae: complete nucleotide sequence and genetic organization","volume":"163","author":"Korch, C.; Hagblom, P.; Ohman, H.","year":"1985","unstructured":"Korch C, Hagblom P, Ohman H, et al. Cryptic plasmid of Neisseria gonorrhoeae: complete nucleotide sequence and genetic organization. J Bacteriol 1985;163:430\u20138.","journal-title":"J Bacteriol"},{"key":"ref_15","article-title":"Molecular cloning: a laboratory manual","author":"Sambrook, J.; Fritsch, E.F.; Maniatis, T.","year":"1983","unstructured":"Sambrook J, Fritsch EF, Maniatis T. 1983. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory, 1983.","edition":"2nd ed","journal-title":"Cold Spring Harbor, NY: Cold Spring Harbor Laboratory"},{"article-title":"Assessing the sensitivity of STD surveillance in the Netherlands: an application of the capture\u2013recapture method. Epidemiol Infect","author":"Reintjes, R.; Termorshuizen, F.; Laar, M.J.W.","key":"ref_16","unstructured":"Reintjes R, Termorshuizen F, Laar MJW. Assessing the sensitivity of STD surveillance in the Netherlands: an application of the capture\u2013recapture method. Epidemiol Infect (in press)."}],"container-title":["Journal of Clinical Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1136\/jcp.52.6.411","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,9,25]],"date-time":"2025-09-25T13:12:04Z","timestamp":1758805924000},"score":1,"resource":{"primary":{"URL":"https:\/\/jcp.bmj.com\/lookup\/doi\/10.1136\/jcp.52.6.411"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1999,6]]},"references-count":16,"journal-issue":{"issue":"6","published-online":{"date-parts":[[1999,6,1]]},"published-print":{"date-parts":[[1999,6]]}},"alternative-id":["10.1136\/jcp.52.6.411"],"URL":"https:\/\/doi.org\/10.1136\/jcp.52.6.411","relation":{},"ISSN":["0021-9746","1472-4146"],"issn-type":[{"type":"print","value":"0021-9746"},{"type":"electronic","value":"1472-4146"}],"subject":[],"published":{"date-parts":[[1999,6]]}}}