{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,12]],"date-time":"2026-02-12T15:42:42Z","timestamp":1770910962730,"version":"3.50.1"},"reference-count":13,"publisher":"BMJ","issue":"8","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clin Pathol"],"published-print":{"date-parts":[[1999,8]]},"abstract":"<jats:p>AIMS: To determine whether quantitative blood culture methods could improve the diagnosis of septicaemic melioidosis. METHODS: A comparison of conventional broth based blood cultures, a pour plate method, and a commercial lysis centrifugation (Isolator 10) blood culture system was conducted in 71 Thai patients with severe melioidosis. The time to identification of B pseudomallei was recorded for each method. RESULTS: 42 patients (59%) were septicaemic. Compared with conventional blood culture, the Isolator and pour plate methods had sensitivities of 81% and 61%, respectively. The median times to a positive culture were: Isolator 39.3 hours, pour plates 45.5 hours, broth culture 61.8 hours (p &lt; 0.001 Isolator v broth). There was a significant inverse correlation between Isolator tube or pour plate quantitative counts and time to detection (r = -0.44 and -0.57, respectively). Mortality was higher in patients who were septicaemic. CONCLUSIONS: Routine use of one of these quantitative methods, in addition to conventional broth culture, may lead to earlier diagnosis of septicaemic melioidosis.<\/jats:p>","DOI":"10.1136\/jcp.52.8.616","type":"journal-article","created":{"date-parts":[[2008,12,31]],"date-time":"2008-12-31T19:05:02Z","timestamp":1230750302000},"page":"616-619","source":"Crossref","is-referenced-by-count":13,"title":["A comparison of lysis centrifugation, pour plate, and conventional blood culture methods in the diagnosis of septicaemic melioidosis."],"prefix":"10.1136","volume":"52","author":[{"given":"A J","family":"Simpson","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P A","family":"Howe","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"V","family":"Wuthiekanun","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N J","family":"White","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[1999,8,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1128\/CMR.4.1.52","article-title":"Melioidosis: the tip of the iceberg?","volume":"4","author":"Dance, D.A.B.","year":"1991","unstructured":"Dance DAB. 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The epidemiology of melioidosis in Ubon Ratchatani, northeast Thailand. Int J Epidemiol 1994;23:1082\u201390.","journal-title":"Int J Epidemiol"},{"key":"ref_4","first-page":"697","article-title":"Halving of mortality of severe melioidosis by ceftazidime","volume":"ii:","author":"White, N.J.; Dance, D.A.B.; Chaowagul, W.","year":"1989","unstructured":"White NJ, Dance DAB, Chaowagul W, et al. Halving of mortality of severe melioidosis by ceftazidime. Lancet 1989;ii:697\u2013701.","journal-title":"Lancet"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1136\/jcp.47.4.377","article-title":"Immunofluorescence microscopy for the rapid diagnosis of melioidosis","volume":"47","author":"Walsh, A.L.; Smith, M.D.; Wuthiekanun, V.","year":"1994","unstructured":"Walsh AL, Smith MD, Wuthiekanun V, et al. Immunofluorescence microscopy for the rapid diagnosis of melioidosis. 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Clinical comparison of the Isolator and BacT\/Alert aerobic blood culture systems. J Clin Microbiol 1995;33:1878\u201390.","journal-title":"J Clin Microbiol"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"96","DOI":"10.4269\/ajtmh.1997.57.96","article-title":"Speed of detection of Burkholderia pseudomallei in blood cultures and its correlation with the clinical outcome","volume":"57","author":"Tiangpitayakorn, C.; Songsivilai, S.; Piyasangthong, N.","year":"1997","unstructured":"Tiangpitayakorn C, Songsivilai S, Piyasangthong N, et al. Speed of detection of Burkholderia pseudomallei in blood cultures and its correlation with the clinical outcome. 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