{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T09:40:52Z","timestamp":1790761252796,"version":"4.1.0"},"reference-count":11,"publisher":"BMJ","issue":"9","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clin Pathol"],"published-print":{"date-parts":[[1999,9]]},"abstract":"<jats:p>BACKGROUND: Ro\/SS-A autoantibodies associated with systemic lupus erythematosus (SLE) and Sj\u00f6gren syndrome may be missed during routine screening for antinuclear autoantibodies (ANA) by immunofluorescence using HEp-2 cells. AIMS: To investigate the use of HEp-2 cells transfected with human 60 kDa Ro\/SS-A for routine detection of these antibodies. METHODS: 10,500 sera were screened at a dilution of 1:200 for Ro\/SS-A antibodies, identified by intense immunofluorescence staining in 10-15% of hyperexpressing cells of either the nucleus and nucleolus combined or the nucleus alone. RESULTS: Ro\/SS-A antibodies were identified in 160\/2100 ANA positive sera (8%), of which seven were ANA negative (titre &lt; 200) and 33 had weak ANA titres (200) in 85-90% of non-hyperexpressing \"background\" cells. Enzyme linked immunosorbent assay (ELISA) confirmed the presence of Ro\/SS-A antibodies in 110 newly diagnosed Ro\/SS-A positive sera. Of these, 50 reacted with Ro\/SS-A, 51 with Ro\/SS-A and La\/SS-B, and nine with Ro\/SS-A and other extractable nuclear antigen (ENA) specificities. Fifteen sera which did not show Ro\/SS-A antibodies by immunofluorescence tested positive for Ro\/SS-A by immunodiffusion, counter-immunoelectrophesis, or ELISA; of these, 14 had ANA titres &gt; 200. Clinical data from 95 Ro\/SS-A positive patients showed that 52% had SLE, 24% Sj\u00f6gren syndrome, 8% rheumatoid arthritis, and 16% other diseases. CONCLUSIONS: (1) HEp-2 cells transfected with human 60 kDa Ro\/SSA are useful for routine immunofluorescence detection for Ro\/SS-A antibodies with a positive predictive value of 100%; (2) sera positive for Ro\/SS-A antibodies by immunofluorescence should be tested for ENA by other methods because &gt; 50% of these sera will have another ENA reactivity in addition to Ro\/SS-A; (3) detection of Ro\/SS-A by immunofluorescence may be missed in the presence of high titre ANAs; (4) with a detection sensitivity of 91%, a negative immunofluorescence results for Ro\/SS-A does not exclude the presence of this autoantibody.<\/jats:p>","DOI":"10.1136\/jcp.52.9.684","type":"journal-article","created":{"date-parts":[[2008,12,31]],"date-time":"2008-12-31T19:05:02Z","timestamp":1230750302000},"page":"684-687","source":"Crossref","is-referenced-by-count":36,"title":["Routine immunofluorescence detection of Ro\/SS-A autoantibody using HEp-2 cells transfected with human 60 kDa Ro\/SS-A."],"prefix":"10.1136","volume":"52","author":[{"given":"W","family":"Pollock","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B H","family":"Toh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"239","published-online":{"date-parts":[[1999,9,1]]},"reference":[{"key":"ref_1","first-page":"93","article-title":"Antinuclear antibodies: diagnostic markers for autoimmune diseases and probes for cell biology Adv Immunol","volume":"44","author":"Tan, E.M.","year":"1989","unstructured":"Tan EM. 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