{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,13]],"date-time":"2025-12-13T07:03:17Z","timestamp":1765609397854,"version":"3.45.0"},"reference-count":25,"publisher":"BMJ","issue":"9","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clin Pathol"],"published-print":{"date-parts":[[1999,9]]},"abstract":"<jats:p>AIMS: To assess the value of immunophenotyping of acute lymphoblastic leukaemia (ALL) in routinely processed bone marrow trephine biopsy specimens and to establish a minimum panel of antibodies to assess lymphoid lineage and enable differentiation from acute myeloid leukaemia. METHODS: 45 routinely processed bone marrow biopsy specimens (formalin fixed, paraffin embedded and mildly decalcified in EDTA) reported to contain leukaemic infiltrates on the basis of cytomorphological and enzyme-cytochemical analysis of bone marrow smears (22 c-ALL, 11 T-ALL, 2 B-ALL, 10 u-ALL (unclassified)) were immunostained by the ABC method with a broad panel of 26 antibodies against various haemopoietic antigens. RESULTS: Staining with antibodies directed against myeloperoxidase and lysozyme showed that seven cases were either biphenotypic or mixed leukaemias (2), or of myelogenous origin (acute myeloid leukaemia (AML)-M1 (2); AML-M4 (2); AML-M5a (1)). Five of these seven cases had been diagnosed initially as u-ALL. Three further cases with no compact leukaemic infiltrates were excluded. ALL was confirmed in the remaining 35 cases. Because of revised diagnoses, the total numbers of ALL subtypes changed (23 c-ALL, 8 T-ALL, 2 B-ALL, 2 u-ALL). Immunostaining of more than 10% of blast cells in at least one case was found with 19 of the 26 antibodies. The most sensitive lineage specific antibodies for diagnosis were found to be anti-CD10 for c-ALL (22\/23) and beta F1 for T-ALL (6\/8). Expression of aberrant antigens was fairly common--for example, 7\/23 cases of c-ALL stained with antibodies against T cell associated antigens. CONCLUSIONS: Immunohistochemical investigation of routinely processed bone marrow biopsy specimens enables reliable detection of ALL subtypes c-ALL and T-ALL. A minimum panel of antibodies, against TdT, CD34, myeloperoxidase, lysozyme, CD10, CD79a, and CD20, and the antibody beta F1, is proposed for the immunophenotyping of acute leukaemia.<\/jats:p>","DOI":"10.1136\/jcp.52.9.688","type":"journal-article","created":{"date-parts":[[2008,12,31]],"date-time":"2008-12-31T19:05:02Z","timestamp":1230750302000},"page":"688-692","source":"Crossref","is-referenced-by-count":23,"title":["Immunophenotyping of acute lymphoblastic leukaemia in routinely processed bone marrow biopsy specimens."],"prefix":"10.1136","volume":"52","author":[{"given":"B","family":"Toth","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M","family":"Wehrmann","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E","family":"Kaiserling","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H P","family":"Horny","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[1999,9,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"362","DOI":"10.1093\/ajcp\/99.4.362","article-title":"Immunophenotyping of lymphomas and leukemias in paraYn-embedded tissues","volume":"99","author":"Perkins, S.L.; Kjeldsberg, C.R.","year":"1993","unstructured":"Perkins SL, Kjeldsberg CR. Immunophenotyping of lymphomas and leukemias in paraYn-embedded tissues. Am J Clin Pathol 1993;99:362\u201373.","journal-title":"Am J Clin Pathol"},{"key":"ref_2","first-page":"181","article-title":"Immunophenotypic criteria for the diagnosis of non-Hodgkin\u2019s lymphoma","volume":"128","author":"Picker, L.J.; Weiss, L.M.; Medeiros, L.J.","year":"1987","unstructured":"Picker LJ, Weiss LM, Medeiros LJ, et al. Immunophenotypic criteria for the diagnosis of non-Hodgkin\u2019s lymphoma. Am J Pathol 1987;128:181\u2013201.","journal-title":"Am J Pathol"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"579","DOI":"10.1093\/ajcp\/91.5.579","article-title":"Immunophenotypic diagnosis of non-Hodgkin\u2019s lymphoma in paraYn sections: coexpression of L60 (Leu22) and L26 antigens correlates with malignant histologic findings","volume":"91","author":"Ngan, B.Y.; Picker, L.J.; Medeiros, L.J.","year":"1989","unstructured":"Ngan BY, Picker LJ, Medeiros LJ, et al. Immunophenotypic diagnosis of non-Hodgkin\u2019s lymphoma in paraYn sections: coexpression of L60 (Leu22) and L26 antigens correlates with malignant histologic findings. Am J Clin Pathol 1989; 91:579\u201383.","journal-title":"Am J Clin Pathol"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"517","DOI":"10.1093\/ajcp\/95.4.517","article-title":"Immunophenotyping of non-Hodgkin\u2019s lymphoma in paraYnembedded tissue sections. A comparison with genotypic analysis","volume":"95","author":"Elghetany, M.T.; Kurec, A.S.; Schuehler, K.","year":"1991","unstructured":"Elghetany MT, Kurec AS, Schuehler K, et al. Immunophenotyping of non-Hodgkin\u2019s lymphoma in paraYnembedded tissue sections. A comparison with genotypic analysis. Am J Clin Pathol 1991;95:517\u201325.","journal-title":"Am J Clin Pathol"},{"key":"ref_5","first-page":"17","article-title":"Immunophenotyping of hematologic neoplasms in paraYn-embedded tissue sections","volume":"93","author":"Davey, F.R.; Elghetany, M.T.; Kurec, A.S.","year":"1990","unstructured":"Davey FR, Elghetany MT, Kurec AS. Immunophenotyping of hematologic neoplasms in paraYn-embedded tissue sections. Am J Clin Pathol 1990;93:S17\u201326.","journal-title":"Am J Clin Pathol"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1158","DOI":"10.1016\/S0046-8177(97)90254-9","article-title":"TdT, bcl-2, and CD34 expression in paraYn-embedded high-grade lymphoma\/acute lymphoblastic leukemia distinguishes between distinct clinicopathologic entities","volume":"28","author":"Soslow, R.A.; Bhargava, V.; MIC2, Warnke R.A.","year":"1997","unstructured":"Soslow RA, Bhargava V, Warnke RA. MIC2, TdT, bcl-2, and CD34 expression in paraYn-embedded high-grade lymphoma\/acute lymphoblastic leukemia distinguishes between distinct clinicopathologic entities. Hum Pathol 1997;28:1158\u201365.","journal-title":"Hum Pathol"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"502","DOI":"10.1093\/ajcp\/93.4.502","article-title":"Immunophenotyping of acute leukemias using paraYn-embedded tissue sections","volume":"93","author":"Kurec, A.S.; Cruz, V.E.; Barrett, D.","year":"1990","unstructured":"Kurec AS, Cruz VE, Barrett D, et al. Immunophenotyping of acute leukemias using paraYn-embedded tissue sections. Am J Clin Pathol 1990;93:502\u20139.","journal-title":"Am J Clin Pathol"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"810","DOI":"10.1016\/0046-8177(94)90251-8","article-title":"Assessment of the value of immunohistochemistry in the subtyping of acute leukemia on routinely processed bone marrow biopsy specimens with particular reference to macrophageassociated antibodies","volume":"25","author":"Horny, H.-P.; Wehrmann, M.; Steinke, B.","year":"1994","unstructured":"Horny H-P, Wehrmann M, Steinke B, et al. Assessment of the value of immunohistochemistry in the subtyping of acute leukemia on routinely processed bone marrow biopsy specimens with particular reference to macrophageassociated antibodies. Hum Pathol 1994;25:810\u201314.","journal-title":"Hum Pathol"},{"key":"ref_9","first-page":"119","article-title":"Immunohistochemical characterization of acute leukemia. Study of 31 bone marrow biopsies","volume":"15","author":"Rousselet, M.C.; Laniece, A.; Gardais, J.","year":"1995","unstructured":"Rousselet MC, Laniece A, Gardais J, et al. Immunohistochemical characterization of acute leukemia. Study of 31 bone marrow biopsies. Ann Pathol 1995;15:119\u201326."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"410","DOI":"10.1093\/ajcp\/107.4.410","article-title":"Useful panel of antibodies for the classification of acute leukemia by immunohistochemical methods in bone marrow trephine biopsy specimens","volume":"107","author":"Shih-Sung, Chuang; Chin-Yang, Li","year":"1997","unstructured":"Chuang Shih-Sung, Li Chin-Yang. Useful panel of antibodies for the classification of acute leukemia by immunohistochemical methods in bone marrow trephine biopsy specimens. Am J Clin Pathol 1997;107:410\u201318.","journal-title":"Am J Clin Pathol"},{"key":"ref_11","first-page":"582","article-title":"Terminal deoxynucleotidyl transferase staining of malignant lymphomas in paraYn sections","volume":"7","author":"Orazi, A.; Cattoretti, G.; John, K.","year":"1994","unstructured":"Orazi A, Cattoretti G, John K, et al. Terminal deoxynucleotidyl transferase staining of malignant lymphomas in paraYn sections. Mod Pathol 1994;7:582\u20136.","journal-title":"Mod Pathol"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"385","DOI":"10.3109\/10428199509059636","article-title":"CD10 antigen in leukemia and lymphoma diagnosis and immunotherapy","volume":"18","author":"Scheuermann, R.H.; Raolla, E.","year":"1995","unstructured":"Scheuermann RH, Raolla E. CD10 antigen in leukemia and lymphoma diagnosis and immunotherapy. Leuk Lymphoma 1995;18:385\u201397.","journal-title":"Leuk Lymphoma"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"449","DOI":"10.1111\/j.1365-2141.1988.tb02397.x","article-title":"Lymphoid cells and tissue mast cells of bone marrow lesions in systemic mastocytosis: a histological and immunohistological study","volume":"69","author":"Horny, H.-P.; Kaiserling, E.","year":"1998","unstructured":"Horny H-P, Kaiserling E. Lymphoid cells and tissue mast cells of bone marrow lesions in systemic mastocytosis: a histological and immunohistological study. Br J Haematol 1998;69:449\u201355.","journal-title":"Br J Haematol"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1111\/j.1365-2141.1989.tb04286.x","article-title":"In situ immunophenotyping of lymphocytes in human bone marrow: an immunohistochemical study","volume":"71","author":"Horny, H.-P.; Engst, U.; Walz, R.S.","year":"1989","unstructured":"Horny H-P, Engst U, Walz RS, et al. In situ immunophenotyping of lymphocytes in human bone marrow: an immunohistochemical study. Br J Haematol 1989;71:313\u2013 21.","journal-title":"Br J Haematol"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"577","DOI":"10.1177\/29.4.6166661","article-title":"Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures","volume":"29","author":"Hsu, S.; Raine, L.; Fanger, H.","year":"1981","unstructured":"Hsu S, Raine L, Fanger H. Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures. J Histochem Cytochem 1981;29:577\u201380.","journal-title":"J Histochem Cytochem"},{"key":"ref_16","first-page":"733","article-title":"Myeloperoxidase: a specific marker for myeloid cells in paraYn sections","volume":"4","author":"Pinkus, G.S.; Pinkus, J.L.","year":"1991","unstructured":"Pinkus GS, Pinkus JL. Myeloperoxidase: a specific marker for myeloid cells in paraYn sections. Mod Pathol 1991;4: 733\u201341.","journal-title":"Mod Pathol"},{"key":"ref_17","first-page":"265","article-title":"The CD34 haemopoietic progenitor cell associated antigen: biology and clinical applications","volume":"77","author":"Silvestri, F.; Banavali, S.; Baccarani, M.","year":"1992","unstructured":"Silvestri F, Banavali S, Baccarani M, et al. The CD34 haemopoietic progenitor cell associated antigen: biology and clinical applications. Haematologica 1992;77:265\u201373.","journal-title":"Haematologica"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1226","DOI":"10.1182\/blood.V83.5.1226.1226","article-title":"Signaling and induction of enhanced cytoadhesiveness via the haematopoietic progenitor cell surface molecule CD34","volume":"83","author":"Majdic, O.; St\u00f6ckl, J.; Pickl, W.","year":"1994","unstructured":"Majdic O, St\u00f6ckl J, Pickl W. Signaling and induction of enhanced cytoadhesiveness via the haematopoietic progenitor cell surface molecule CD34. Blood 1994;83:1226\u2013 34.","journal-title":"Blood"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1136\/jcp.48.4.291","article-title":"QBEND10 for the diagnosis of myelodysplastic syndromes in routinely-processed bone marrow biopsy specimens","volume":"48","author":"Horny, H.-P.; Wehrmann, M.; Schlicker, H.U.H.","year":"1995","unstructured":"Horny H-P, Wehrmann M, Schlicker HUH, et al. QBEND10 for the diagnosis of myelodysplastic syndromes in routinely-processed bone marrow biopsy specimens. J Clin Pathol 1995;48:291\u20134.","journal-title":"J Clin Pathol"},{"key":"ref_20","first-page":"415","article-title":"Utilization of monoclonal antibody L26 in the identification and confirmation of B-cell lymphomas","volume":"129","author":"Cartun, R.W.; Coles, F.B.; Pastuszak, W.T.","year":"1987","unstructured":"Cartun RW, Coles FB, Pastuszak WT. Utilization of monoclonal antibody L26 in the identification and confirmation of B-cell lymphomas. Am J Pathol 1987;129:415\u201321.","journal-title":"Am J Pathol"},{"key":"ref_21","first-page":"134","article-title":"Reactivity and specificity of L26 (pan-B-cell mAb) on 322 cases of fresh and paraYnembedded lymphoproliferative diseases","author":"Takami, T.; Qi, C.; Yamada, T.","year":"1989","unstructured":"Takami T, Qi C, Yamada T. Reactivity and specificity of L26 (pan-B-cell mAb) on 322 cases of fresh and paraYnembedded lymphoproliferative diseases. In: Knapp W, ed. Abstracts from 4th International Conference on Human Leucocyte DiVerentiation Antigens, Vienna, 21\u201325 Feb 1989. Copenhagen: Munksgaard, 1989:134\u20136."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1093\/ajcp\/93.1.58","article-title":"Swerdlow SH. Detection of B-and T-cells in paraYn-embedded tissue sections. Diagnostic utility of commercially obtained 4KB5 and UCHL-1","volume":"93","author":"Clark, JR, Williams; M.E.","year":"1990","unstructured":"Clark JR, Williams ME, Swerdlow SH. Detection of B-and T-cells in paraYn-embedded tissue sections. Diagnostic utility of commercially obtained 4KB5 and UCHL-1. Am J Clin Pathol 1990;93:58\u201369.","journal-title":"Am J Clin Pathol"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"853","DOI":"10.1182\/blood.V82.3.853.bloodjournal823853","article-title":"mb- 1: a new marker for B-lineage lymphoblastic leukemia","volume":"82","author":"Buccheri, V.; Mihaljevic, B.; Matutes, E.","year":"1993","unstructured":"Buccheri V, Mihaljevic B, Matutes E, et al. mb-1: a new marker for B-lineage lymphoblastic leukemia. Blood 1993;82:853\u20137.","journal-title":"Blood"},{"key":"ref_24","first-page":"1939","article-title":"Transcription and protein expression of mb-1 and B29 genes in human hematopoetic malignancies and cell lines","volume":"7","author":"Verschuren, M.C.M.; Comans-Bitter, W.M.; Kapteijn, C.A.C.","year":"1993","unstructured":"Verschuren MCM, Comans-Bitter WM, Kapteijn CAC, et al. Transcription and protein expression of mb-1 and B29 genes in human hematopoetic malignancies and cell lines. Leukemia 1993;7:1939\u201347.","journal-title":"Leukemia"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1453","DOI":"10.1182\/blood.V86.4.1453.bloodjournal8641453","article-title":"CD79a: a novel marker for B-cell neoplasms in routinely-processed tissue samples","volume":"86","author":"Mason, D.Y.; Cordell, J.L.; Brown, M.H.","year":"1995","unstructured":"Mason DY, Cordell JL, Brown MH, et al. CD79a: a novel marker for B-cell neoplasms in routinely-processed tissue samples. 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