{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,14]],"date-time":"2025-12-14T11:44:53Z","timestamp":1765712693296,"version":"3.48.0"},"reference-count":28,"publisher":"BMJ","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clin Pathol"],"published-print":{"date-parts":[[2000,4]]},"abstract":"<jats:p>\n                    <jats:italic>Aims<\/jats:italic>\n                    \u2014To investigate the association of cagA positivity and non-opsonic neutrophil activation capacity in wild-type Helicobacter pylori strains with peptic ulcer disease or chronic gastritis only.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:italic>Methods<\/jats:italic>\n                    \u2014\n                    <jats:italic>Helicobacter pylori<\/jats:italic>\n                    were isolated from antral biopsies of 53 consecutive patients with chronic antral gastritis, of whom 24 had peptic ulcer disease endoscopically. The presence of cagA, a marker for the cag pathogenicity island, was determined by polymerase chain reaction with specific oligonucleotide primers, and non-opsonic neutrophil activation capacity by luminol enhanced chemiluminescence.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:italic>Results<\/jats:italic>\n                    \u2014The cagA gene was present in 39 of 53 (73.6%) strains, 20 of which (83.3%) were from the 24 patients with peptic ulcer disease and 19 (65.5%) from the 29 patients with chronic gastritis only. Non-opsonic neutrophil activation was found in 29 (54.7%) strains, 16 of which (66.7%) were from patients with peptic ulcer disease, and 13 (44.8%) from those with chronic gastritis. Non-opsonic neutrophil activation was found more frequently in cagA\n                    <jats:sup>+<\/jats:sup>\n                    than cagA\n                    <jats:sup>\u2212<\/jats:sup>\n                    strains (59% v 42.9%). Whereas four of the 14 cagA\n                    <jats:sup>\u2212<\/jats:sup>\n                    strains and eight of the 24 non-opsonic neutrophil activation negative strains were from patients with peptic ulcer disease, only two of 24 (8.3%) peptic ulcer disease strains expressed neither cagA nor non-opsonic neutrophil activation. The cagA gene and non-opsonic neutrophil activation capacity were co-expressed in 14 of 24 (58.3%) strains from patients with peptic ulcer disease, and in nine of 29 (31%) strains from individuals with chronic gastritis.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:italic>Conclusions<\/jats:italic>\n                    \u2014Positivity for cagA and non-opsonic neutrophil activation occur independently in wild-type\n                    <jats:italic>H pylori<\/jats:italic>\n                    strains. However, co-expression of the two markers enhanced the prediction of peptic ulcer disease.\n                  <\/jats:p>","DOI":"10.1136\/jcp.53.4.318","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T06:37:59Z","timestamp":1027751879000},"page":"318-321","source":"Crossref","is-referenced-by-count":13,"title":["Co-expression in\n                    <i>Helicobacter pylori<\/i>\n                    of cagA and non-opsonic neutrophil activation enhances the association with peptic ulcer disease"],"prefix":"10.1136","volume":"53","author":[{"given":"D","family":"Danielsson","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S 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