{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,26]],"date-time":"2025-09-26T00:15:25Z","timestamp":1758845725031,"version":"3.44.0"},"reference-count":5,"publisher":"BMJ","issue":"10","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clin Pathol"],"published-print":{"date-parts":[[2003,10]]},"abstract":"<jats:p>\n                  <jats:bold>Background:<\/jats:bold> The need for urgent antineutrophil cytoplasmic antibody (ANCA) results when assessing patients with acute renal failure, pulmonary renal syndrome, or mononeuritis multiplex has led to the development of a rapid qualitative ELISA screening assay for antibodies to myeloperoxidase (MPO) and proteinase 3 (PR3).<\/jats:p>\n               <jats:p>\n                  <jats:bold>Aims:<\/jats:bold> To report the use of a rapid qualitative ELISA screen for PR3-ANCA and MPO-ANCA in a regional immunology laboratory and its correlation with standard indirect immunofluorescence (IIF) and quantitative ELISA for PR3-ANCA and MPO-ANCA.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Methods:<\/jats:bold> Over 12 months, 103 samples requiring urgent ANCA testing were screened by a rapid qualitative ELISA and the results compared with IIF and quantitative ELISA assays for PR3-ANCA and MPO-ANCA.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Results:<\/jats:bold> There was an excellent correlation between the rapid qualitative ELISA and standard ANCA IIF and a routine ELISA for MPO\/PR3-ANCA, with sensitivities ranging from 82% to 100%. There were two false negatives, which gave weak to moderately positive values as determined by routine ELISA. However, the clinical relevance of these two cases is doubtful.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Conclusions:<\/jats:bold> The rapid ELISA for anti-MPO and anti-PR3 correlates well with quantitative ELISA and IIF ANCA, and urgent management decisions in patients with suspected small vessel vasculitis can be based with confidence on this test.<\/jats:p>","DOI":"10.1136\/jcp.56.10.775","type":"journal-article","created":{"date-parts":[[2003,9,26]],"date-time":"2003-09-26T16:27:04Z","timestamp":1064593624000},"page":"775-777","source":"Crossref","is-referenced-by-count":4,"title":["Audit of the clinical usefulness of a rapid qualitative ELISA screen for antimyeloperoxidase and antiproteinase 3 antibodies in the assessment of patients with suspected vasculitis"],"prefix":"10.1136","volume":"56","author":[{"given":"A","family":"Aslam","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T L","family":"Newman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S A","family":"Misbah","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[2003,9,26]]},"reference":[{"key":"2025092503393557000_56.10.775.1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1755.2000.00080.x"},{"key":"2025092503393557000_56.10.775.2","doi-asserted-by":"crossref","first-page":"507","DOI":"10.1093\/ajcp\/111.4.507","volume":"111","year":"1999","journal-title":"Am J Clin Pathol"},{"key":"2025092503393557000_56.10.775.3","doi-asserted-by":"publisher","DOI":"10.1093\/ndt\/12.9.1863"},{"key":"2025092503393557000_56.10.775.4","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1755.2000.057003846.x"},{"key":"2025092503393557000_56.10.775.5","doi-asserted-by":"publisher","DOI":"10.1002\/1529-0131(200012)13:6<424::AID-ART14>3.0.CO;2-Q"}],"container-title":["Journal of Clinical Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1136\/jcp.56.10.775","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,9,25]],"date-time":"2025-09-25T10:39:41Z","timestamp":1758796781000},"score":1,"resource":{"primary":{"URL":"https:\/\/jcp.bmj.com\/lookup\/doi\/10.1136\/jcp.56.10.775"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,9,26]]},"references-count":5,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2003,9,26]]},"published-print":{"date-parts":[[2003,10]]}},"alternative-id":["10.1136\/jcp.56.10.775"],"URL":"https:\/\/doi.org\/10.1136\/jcp.56.10.775","relation":{},"ISSN":["0021-9746","1472-4146"],"issn-type":[{"type":"print","value":"0021-9746"},{"type":"electronic","value":"1472-4146"}],"subject":[],"published":{"date-parts":[[2003,9,26]]}}}