{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T16:32:55Z","timestamp":1791045175634,"version":"4.1.0"},"reference-count":40,"publisher":"BMJ","issue":"9","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clin Pathol"],"published-print":{"date-parts":[[2003,9]]},"abstract":"<jats:p>\n                    <jats:bold>Aims:<\/jats:bold>\n                    To define epidemiology, clinical disease, and outcome of gemella bacteraemia by 16S rRNA gene sequencing. To examine the usefulness of the Vitek, API, and ATB systems in identifying two gemella species.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Methods:<\/jats:bold>\n                    All \u03b1 haemolytic streptococci other than\n                    <jats:italic>Streptococcus pneumoniae<\/jats:italic>\n                    isolated from blood cultures during a six year period were identified by conventional biochemical methods, the Vitek system, and the API system. 16S rRNA gene sequencing was performed on all isolates identified by both kits as gemella with \u2265 95% confidence or by either kit as any bacterial species with &lt; 95% confidence. The ATB expression system was used to identify the two isolates that were defined as gemella species by 16S rRNA gene sequencing.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Results:<\/jats:bold>\n                    Of the 302 \u03b1 haemolytic streptococci other than\n                    <jats:italic>S pneumoniae<\/jats:italic>\n                    isolated, one was identified as\n                    <jats:italic>Gemella morbillorum<\/jats:italic>\n                    , and another as\n                    <jats:italic>Gemella haemolysans<\/jats:italic>\n                    by 16S rRNA gene sequencing. The patient with monomicrobial\n                    <jats:italic>G morbillorum<\/jats:italic>\n                    bacteraemia was a 66 year old man with community acquired infective endocarditis with septic thromboemboli. The patient with\n                    <jats:italic>G haemolysans<\/jats:italic>\n                    bacteraemia was a 41 year old woman with hospital acquired polymicrobial bacteraemia during the neutropenic period of an autologous bone marrow transplant for non-Hodgkin\u2019s lymphoma, the first case of its kind in the English literature. The API and ATB expression systems only identified the second strain as\n                    <jats:italic>G haemolysans<\/jats:italic>\n                    at 94% and 99% confidence, respectively, whereas the Vitek system identified none of the two strains correctly at &gt; 70% confidence.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Conclusions:<\/jats:bold>\n                    Gemella bacteraemia is uncommon. 16S rRNA gene sequencing is the method of choice for identification of gemella and gemella-like isolates.\n                  <\/jats:p>","DOI":"10.1136\/jcp.56.9.690","type":"journal-article","created":{"date-parts":[[2003,8,27]],"date-time":"2003-08-27T18:39:43Z","timestamp":1062009583000},"page":"690-693","source":"Crossref","is-referenced-by-count":70,"title":["Gemella bacteraemia characterised by 16S ribosomal RNA gene sequencing"],"prefix":"10.1136","volume":"56","author":[{"given":"P C Y","family":"Woo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S K P","family":"Lau","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A M Y","family":"Fung","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S K","family":"Chiu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R W H","family":"Yung","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K Y","family":"Yuen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"239","published-online":{"date-parts":[[2003,8,27]]},"reference":[{"key":"2025112110465817000_56.9.690.1","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1096\/fasebj.7.1.8422957","volume":"7","year":"1993","journal-title":"FASEB J"},{"key":"2025112110465817000_56.9.690.2","doi-asserted-by":"publisher","DOI":"10.1136\/mp.53.4.211"},{"key":"2025112110465817000_56.9.690.3","doi-asserted-by":"publisher","DOI":"10.1136\/mp.54.4.244"},{"key":"2025112110465817000_56.9.690.4","doi-asserted-by":"publisher","DOI":"10.1016\/S0732-8893(01)00206-1"},{"key":"2025112110465817000_56.9.690.5","doi-asserted-by":"publisher","DOI":"10.1016\/S0732-8893(02)00375-9"},{"key":"2025112110465817000_56.9.690.6","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.1.265-267.2002"},{"key":"2025112110465817000_56.9.690.7","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.3.1190-1194.2001"},{"key":"2025112110465817000_56.9.690.8","doi-asserted-by":"crossref","first-page":"740","DOI":"10.1099\/0022-1317-51-9-740","volume":"51","year":"2002","journal-title":"J Med Microbiol"},{"key":"2025112110465817000_56.9.690.9","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.2.613-618.2003"},{"key":"2025112110465817000_56.9.690.10","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.3.1004-1009.2003"},{"key":"2025112110465817000_56.9.690.11","doi-asserted-by":"crossref","first-page":"3515","DOI":"10.1128\/JCM.38.9.3515-3517.2000","volume":"38","year":"2000","journal-title":"J Clin Microbiol"},{"key":"2025112110465817000_56.9.690.12","doi-asserted-by":"publisher","DOI":"10.1016\/S0732-8893(01)00261-9"},{"key":"2025112110465817000_56.9.690.13","doi-asserted-by":"crossref","first-page":"635","DOI":"10.1099\/0022-1317-51-8-635","volume":"51","year":"2002","journal-title":"J Med Microbiol"},{"key":"2025112110465817000_56.9.690.14","doi-asserted-by":"publisher","DOI":"10.1136\/mp.55.3.182"},{"key":"2025112110465817000_56.9.690.15","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.7.2681-2685.2002"},{"key":"2025112110465817000_56.9.690.16","first-page":"289","volume":"192","year":"2001","journal-title":"J Pathol"},{"key":"2025112110465817000_56.9.690.17","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM200109133451119"},{"key":"2025112110465817000_56.9.690.18","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.4.1219-1224.2002"},{"key":"2025112110465817000_56.9.690.19","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.12.4227-4232.2001"},{"key":"2025112110465817000_56.9.690.20","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.3.805-810.2002"},{"key":"2025112110465817000_56.9.690.21","doi-asserted-by":"publisher","DOI":"10.1136\/mp.56.1.29"},{"key":"2025112110465817000_56.9.690.22","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.9.3147-3155.2001"},{"key":"2025112110465817000_56.9.690.23","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1099\/jmm.0.04950-0","volume":"52","year":"2003","journal-title":"J Med Microbiol"},{"key":"2025112110465817000_56.9.690.24","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(00)04131-3"},{"key":"2025112110465817000_56.9.690.25","unstructured":"Murray PR, Baro EJ, Pfaller MA, et al. 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