{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T17:02:14Z","timestamp":1784739734911,"version":"3.55.0"},"reference-count":19,"publisher":"SAGE Publications","issue":"2","license":[{"start":{"date-parts":[[2018,2,1]],"date-time":"2018-02-01T00:00:00Z","timestamp":1517443200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Journal of Investigative Medicine"],"published-print":{"date-parts":[[2018,2]]},"abstract":"<jats:p>Pleural fibrosis can dramatically lower the quality of life. Numerous studies have reported that epithelial-mesenchymal transition (EMT) regulated by transforming growth factor-\u03b2 (TGF-\u03b2) is involved in fibrosis. However, the molecular mechanism is inadequately understood. Fibroblast-specific protein-1 (S100A4) is a target of TGF-\u03b2 signaling. In our previous study, we have reported that S100A4 is highly expressed in pleural fibrosis. Thus, we suggest that S100A4 took part in the TGF-\u03b2-induced EMT in pleural fibrosis. In this study, we determined the expression of S100A4 and EMT-related markers in Met-5A cells (pleural mesothelial cells) treated with TGF-\u03b2 or TGF-\u03b2 inhibitor by real-time PCR and western blot. In order to explore the role of S100A4, we used siRNA to knock down the expression of S100A4 in cell model. We found that the expression of epithelial cell marker was decreased and the mesenchymal cell marker increased with S100A4 upregulation after treatment with TGF-\u03b2. Moreover, the changes of EMT-related event were restricted when the expression of S100A4 was knocked down. Conversely, S100A4 can partially rescue the EMT-related expression changes induced by TGF-\u03b2 inhibitor. These findings suggest that S100A4 expression is induced by the TGF-\u03b2 pathway, and silencing S100A4 expression can inhibit the process of TGF-\u03b2-induced EMT.<\/jats:p>","DOI":"10.1136\/jim-2017-000542","type":"journal-article","created":{"date-parts":[[2017,11,15]],"date-time":"2017-11-15T20:10:10Z","timestamp":1510776610000},"page":"334-339","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":21,"title":["S100A4 Amplifies Tgf-\u03b2-Induced Epithelial-Mesenchymal Transition in a Pleural Mesothelial Cell Line"],"prefix":"10.1177","volume":"66","author":[{"given":"Qian","family":"Ning","sequence":"first","affiliation":[{"name":"Department of Respiration and Critical Care Medicine, The First Hospital Affiliated to School of Medicine of Xi'an Jiaotong University, Shaanxi, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feiyan","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Respiration and Critical Care Medicine, The First Hospital Affiliated to School of Medicine of Xi'an Jiaotong University, Shaanxi, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Respiration and Critical Care Medicine, The First Hospital Affiliated to School of Medicine of Xi'an Jiaotong University, Shaanxi, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Respiration and Critical Care Medicine, The First Hospital Affiliated to School of Medicine of Xi'an Jiaotong University, Shaanxi, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Yao","sequence":"additional","affiliation":[{"name":"Department of Respiration and Critical Care Medicine, The First Hospital Affiliated to School of Medicine of Xi'an Jiaotong University, Shaanxi, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tinghua","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Respiration and Critical Care Medicine, The First Hospital Affiliated to School of Medicine of Xi'an Jiaotong University, Shaanxi, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhongmin","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Respiration and Critical Care Medicine, The First Hospital Affiliated to School of Medicine of Xi'an Jiaotong University, Shaanxi, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2018,2,1]]},"reference":[{"key":"bibr1-jim-2017-000542","doi-asserted-by":"publisher","DOI":"10.1002\/ppul.23226"},{"key":"bibr2-jim-2017-000542","doi-asserted-by":"publisher","DOI":"10.1111\/1751-2980.12266"},{"key":"bibr3-jim-2017-000542","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajpath.2015.04.014"},{"key":"bibr4-jim-2017-000542","doi-asserted-by":"publisher","DOI":"10.1097\/01.mcp.0000230627.88386.b9"},{"key":"bibr5-jim-2017-000542","doi-asserted-by":"publisher","DOI":"10.1186\/s40169-015-0046-5"},{"key":"bibr6-jim-2017-000542","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-6143.2005.00843.x"},{"key":"bibr7-jim-2017-000542","doi-asserted-by":"publisher","DOI":"10.2353\/ajpath.2010.090526"},{"key":"bibr8-jim-2017-000542","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-10-2350"},{"key":"bibr9-jim-2017-000542","first-page":"507","volume":"86","author":"Schneider M","year":"2008","journal-title":"fibrosis and regeneration in diseases? 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