{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T12:08:41Z","timestamp":1777723721702,"version":"3.51.4"},"reference-count":29,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2022,4,1]],"date-time":"2022-04-01T00:00:00Z","timestamp":1648771200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"name":"National Natural Science Foundation Youth Project of China","award":["81800123"],"award-info":[{"award-number":["81800123"]}]},{"name":"National Natural Science Foundation Youth Project of China","award":["81900131"],"award-info":[{"award-number":["81900131"]}]},{"DOI":"10.13039\/501100006606","name":"Tianjin Natural Science Foundation","doi-asserted-by":"crossref","award":["16JCZDJC35300"],"award-info":[{"award-number":["16JCZDJC35300"]}],"id":[{"id":"10.13039\/501100006606","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Scientific Research Project of Tianjin Education Commission","award":["2018KJ043"],"award-info":[{"award-number":["2018KJ043"]}]},{"name":"Scientific Research Project of Tianjin Education Commission","award":["2017KJ195"],"award-info":[{"award-number":["2017KJ195"]}]},{"name":"Scientific Research Project of Tianjin Education Commission","award":["2018KJ045"],"award-info":[{"award-number":["2018KJ045"]}]},{"name":"Science and Technology Project of Tianjin Health Commission Youth Project","award":["QN20006"],"award-info":[{"award-number":["QN20006"]}]},{"name":"Tianjin Municipal Natural Science Foundation Youth Project","award":["18JCQNJC80400"],"award-info":[{"award-number":["18JCQNJC80400"]}]},{"DOI":"10.13039\/501100019065","name":"Tianjin Science and Technology Plan Project","doi-asserted-by":"crossref","award":["20YFZCSY00060"],"award-info":[{"award-number":["20YFZCSY00060"]}],"id":[{"id":"10.13039\/501100019065","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81770110"],"award-info":[{"award-number":["81770110"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Journal of Investigative Medicine"],"published-print":{"date-parts":[[2022,4]]},"abstract":"<jats:p>Iron overload (IO) affected the survival of patients with myelodysplastic syndrome (MDS). Deferasirox (DFX) is widely used in patients with MDS for iron chelation therapy, but is not suitable for MDS patients with severe thrombocytopenia. Eltrombopag (ELT) is a type of thrombopoietin receptor (TPOR) analog used in the treatment of thrombocytopenia. Therefore, we sought to explore the synergistic effects and possible mechanisms of DFX combination with ELT in MDS cells. In our study, the combination of DFX with ELT synergistically inhibited proliferation, induced apoptosis and arrested cell cycle of MDS cells. Through the RNA-sequence and gene set enrichment analysis (GSEA), iron metabolism\u2013related pathway played important roles in apoptosis of SKM-1 cells treated with DFX plus ELT. Transferrin receptor (TFRC) was significantly highly expressed in combination group than that in single agent groups, without affecting TPOR. Furthermore, the apoptosis of the combination group MDS cells could be partially reversed by ferric ammonium citrate (FAC), accompanied with decreased expression of TFRC. These results suggested that the combination of DFX and ELT synergistically induced apoptosis of MDS cells by enhancing iron deprivation\u2013related pathway.<\/jats:p>","DOI":"10.1136\/jim-2021-002147","type":"journal-article","created":{"date-parts":[[2021,12,17]],"date-time":"2021-12-17T12:12:11Z","timestamp":1639743131000},"page":"953-962","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["Deferasirox Combination with Eltrombopag Shows Anti-Myelodysplastic Syndrome Effects by Enhancing iron Deprivation\u2013Related Apoptosis"],"prefix":"10.1177","volume":"70","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3998-9867","authenticated-orcid":false,"given":"Lei","family":"Huang","sequence":"first","affiliation":[{"name":"Hematology, Tianjin Medical University General Hospital, Tianjin, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mengyue","family":"Tian","sequence":"additional","affiliation":[{"name":"Hematology, Tianjin Medical University General Hospital, Tianjin, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2730-1562","authenticated-orcid":false,"given":"Zhaoyun","family":"Liu","sequence":"additional","affiliation":[{"name":"Hematology, Tianjin Medical University General Hospital, Tianjin, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chunyan","family":"Liu","sequence":"additional","affiliation":[{"name":"Hematology, Tianjin Medical University General Hospital, Tianjin, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rong","family":"Fu","sequence":"additional","affiliation":[{"name":"Hematology, Tianjin Medical University General Hospital, Tianjin, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2023,3,13]]},"reference":[{"key":"bibr1-jim-2021-002147","doi-asserted-by":"publisher","DOI":"10.1016\/j.blre.2016.04.003"},{"key":"bibr2-jim-2021-002147","doi-asserted-by":"publisher","DOI":"10.3760\/cma.j.issn.0253-2727.2019.08.021"},{"key":"bibr3-jim-2021-002147","doi-asserted-by":"publisher","DOI":"10.1016\/j.critrevonc.2015.11.017"},{"key":"bibr4-jim-2021-002147","doi-asserted-by":"publisher","DOI":"10.1007\/s10238-019-00592-5"},{"key":"bibr5-jim-2021-002147","doi-asserted-by":"publisher","DOI":"10.1080\/16078454.2019.1666218"},{"key":"bibr6-jim-2021-002147","doi-asserted-by":"publisher","DOI":"10.1007\/s00277-015-2341-z"},{"key":"bibr7-jim-2021-002147","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2018.212332"},{"key":"bibr8-jim-2021-002147","doi-asserted-by":"publisher","DOI":"10.1016\/j.blre.2018.03.002"},{"key":"bibr9-jim-2021-002147","doi-asserted-by":"publisher","DOI":"10.1634\/stemcells.2008-0366"},{"key":"bibr10-jim-2021-002147","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2009-04-219493"},{"key":"bibr11-jim-2021-002147","doi-asserted-by":"publisher","DOI":"10.1080\/10428194.2019.1576873"},{"key":"bibr12-jim-2021-002147","doi-asserted-by":"publisher","DOI":"10.1111\/ejh.12383"},{"key":"bibr13-jim-2021-002147","doi-asserted-by":"publisher","DOI":"10.3109\/10428199509056841"},{"key":"bibr14-jim-2021-002147","doi-asserted-by":"publisher","DOI":"10.3109\/10428199709114174"},{"key":"bibr15-jim-2021-002147","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2014-03-563221"},{"key":"bibr16-jim-2021-002147","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2010.34.1222"},{"key":"bibr17-jim-2021-002147","doi-asserted-by":"publisher","DOI":"10.1016\/S2352-3026(17)30012-1"},{"key":"bibr18-jim-2021-002147","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2018-01-826784"},{"key":"bibr19-jim-2021-002147","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2016-10-740241"},{"key":"bibr20-jim-2021-002147","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2016.146746"},{"key":"bibr21-jim-2021-002147","doi-asserted-by":"publisher","DOI":"10.1080\/10428194.2017.1280604"},{"key":"bibr22-jim-2021-002147","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21207674"},{"key":"bibr23-jim-2021-002147","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2020.593383"},{"key":"bibr24-jim-2021-002147","unstructured":"Luo J, Yan R, He X, Constitutive activation of STAT3 and cyclin D1 overexpression contribute to proliferation, migration and invasion in gastric cancer cells. 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