{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T01:43:04Z","timestamp":1777513384360,"version":"3.51.4"},"reference-count":71,"publisher":"BMJ","issue":"9","license":[{"start":{"date-parts":[[2021,9,29]],"date-time":"2021-09-29T00:00:00Z","timestamp":1632873600000},"content-version":"unspecified","delay-in-days":28,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000738","name":"U.S. Department of Veterans Affairs","doi-asserted-by":"publisher","award":["I01 BX000792-06"],"award-info":[{"award-number":["I01 BX000792-06"]}],"id":[{"id":"10.13039\/100000738","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100011249","name":"Robert H. Lurie Comprehensive Cancer Center","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100011249","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003645","name":"National Cancer Center","doi-asserted-by":"publisher","award":["NCI CA060553"],"award-info":[{"award-number":["NCI CA060553"]}],"id":[{"id":"10.13039\/501100003645","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007155","name":"Walter S. and Lucienne Driskill Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007155","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006595","name":"Unitatea Executiva pentru Finantarea Invatamantului Superior, a Cercetarii, Dezvoltarii si Inovarii","doi-asserted-by":"publisher","award":["PN-III-P1-1.1-TE- 2019-0670"],"award-info":[{"award-number":["PN-III-P1-1.1-TE- 2019-0670"]}],"id":[{"id":"10.13039\/501100006595","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["bmj.com"],"crossmark-restriction":true},"short-container-title":["J Immunother Cancer"],"accepted":{"date-parts":[[2021,9,1]]},"published-print":{"date-parts":[[2021,9]]},"abstract":"<jats:sec>\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>Tissue transglutaminase (TG2), an enzyme overexpressed in cancer cells, promotes metastasis and resistance to chemotherapy. Its distinct effects in cancer versus the host compartments have not been elucidated.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>Here, by using a TG2<jats:sup>-\/-<\/jats:sup> syngeneic ovarian cancer mouse model, we assessed the effects of TG2 deficiency in the host tissues on antitumor immunity and tumor progression. Multicolor flow cytometry was used to phenotype immune cell populations in the peritoneal environment. Cancer cells recovered from malignant ascites were characterized by RNA sequencing, proliferation, and apoptosis assays.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>We observed that host TG2 loss delayed tumor growth and ascites accumulation and caused increased infiltration of CD8<jats:sup>+<\/jats:sup> T cells and decreased numbers of myeloid cells in the peritoneal fluid. Tumor antigen-specific CD8<jats:sup>+<\/jats:sup> T cell cytotoxic responses were enhanced in ascites from TG2<jats:sup>-\/-<\/jats:sup> versus TG2<jats:sup>+\/+<\/jats:sup> mice and CD8<jats:sup>+<\/jats:sup> T cell depletion caused accelerated ascites accumulation in TG2<jats:sup>-\/-<\/jats:sup> mice. CD8<jats:sup>+<\/jats:sup> T cells from tumor-bearing TG2<jats:sup>-\/-<\/jats:sup> mice displayed an effector T cell phenotype, differentiated toward effector memory (T<jats:sub>em<\/jats:sub>). Mechanistically, absence of TG2 augmented signals promoting T cell activation, such as increased cytokine-induced STAT1 and attenuated STAT3 phosphorylation in T cells. Additionally, immune-suppressive myeloid cell populations were reduced in the peritoneal milieu of TG2<jats:sup>-\/-<\/jats:sup> tumor-bearing mice. In response to the more robust immune response caused by loss of TG2, cancer cells growing intraperitoneally exhibited an interferon-\u03b3<\/jats:p>\n                  <jats:p>(IFN-\u03b3) responsive gene signature and underwent apoptosis. In human specimens, stromal, not tumor, TG2 expression correlated indirectly with numbers of tumor-infiltrating lymphocytes.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>Collectively, our data demonstrate decreased tumor burden, increased activation and effector function of T cells, and loss of immunosuppressive signals in the tumor microenvironment of TG2<jats:sup>-\/-<\/jats:sup> mice. We propose that TG2 acts as an attenuator of antitumor T cell immunity and is a new immunomodulatory target.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1136\/jitc-2021-002682","type":"journal-article","created":{"date-parts":[[2021,9,30]],"date-time":"2021-09-30T14:00:53Z","timestamp":1633010453000},"page":"e002682","update-policy":"https:\/\/doi.org\/10.1136\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":26,"title":["Loss of host tissue transglutaminase boosts antitumor T cell immunity by altering STAT1\/STAT3 phosphorylation in ovarian cancer"],"prefix":"10.1136","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3404-4162","authenticated-orcid":false,"given":"Livia Elena","family":"Sima","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology, Northwestern University Feinberg School of Medicine, Chicago, Illinois, USA"},{"name":"Department of Molecular Cell Biology, Institute of Biochemistry of the Romanian Academy, Bucharest, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Siqi","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Medicine; Hematology\/Oncology Division, Northwestern University Feinberg School of Medicine, Chicago, Illinois, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Horacio","family":"Cardenas","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Northwestern University Feinberg School of Medicine, Chicago, Illinois, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1095-8875","authenticated-orcid":false,"given":"Guangyuan","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Northwestern University Feinberg School of Medicine, Chicago, Illinois, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yinu","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Northwestern University Feinberg School of Medicine, Chicago, Illinois, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cristina","family":"Ivan","sequence":"additional","affiliation":[{"name":"Department of Experimental Therapeutics, Center for RNA Interference and Non-Coding RNA, The University of Texas MD Anderson Cancer Center, Houston, Texas, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hao","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Northwestern University Feinberg School of Medicine, Chicago, Illinois, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bin","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Medicine; Hematology\/Oncology Division, Northwestern University Feinberg School of Medicine, Chicago, Illinois, USA"},{"name":"Robert H. 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