{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T00:22:54Z","timestamp":1788308574604,"version":"build-2803163510"},"reference-count":46,"publisher":"BMJ","issue":"3","license":[{"start":{"date-parts":[[2024,3,22]],"date-time":"2024-03-22T00:00:00Z","timestamp":1711065600000},"content-version":"unspecified","delay-in-days":21,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100022721","name":"Villa Joep Foundation","doi-asserted-by":"crossref","award":["IWOV-Actief.51391.180034"],"award-info":[{"award-number":["IWOV-Actief.51391.180034"]}],"id":[{"id":"10.13039\/501100022721","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["bmj.com"],"crossmark-restriction":true},"short-container-title":["J Immunother Cancer"],"accepted":{"date-parts":[[2023,12,18]]},"published-print":{"date-parts":[[2024,3]]},"abstract":"<jats:p>Human leukocyte antigen (HLA) restriction of conventional T-cell targeting introduces complexity in generating T-cell therapy strategies for patients with cancer with diverse HLA-backgrounds. A subpopulation of atypical, major histocompatibility complex-I related protein 1 (MR1)-restricted T-cells, distinctive from mucosal-associated invariant T-cells (MAITs), was recently identified recognizing currently unidentified MR1-presented cancer-specific metabolites. It is hypothesized that the MC.7.G5 MR1T-clone has potential as a pan-cancer, pan-population T-cell immunotherapy approach. These cells are irresponsive to healthy tissue while conferring T-cell receptor(TCR) dependent, HLA-independent cytotoxicity to a wide range of adult cancers. Studies so far are limited to adult malignancies. Here, we investigated the potential of MR1-targeting cellular therapy strategies in pediatric cancer. Bulk RNA sequencing data of primary pediatric tumors were analyzed to assess <jats:italic>MR1<\/jats:italic> expression. <jats:italic>In vitro<\/jats:italic> pediatric tumor models were subsequently screened to evaluate their susceptibility to engineered MC.7.G5 TCR-expressing T-cells. Targeting capacity was correlated with qPCR-based <jats:italic>MR1<\/jats:italic> mRNA and protein overexpression. RNA expression of <jats:italic>MR1<\/jats:italic> in primary pediatric tumors varied widely within and between tumor entities. Notably, embryonal tumors exhibited significantly lower <jats:italic>MR1<\/jats:italic> expression than other pediatric tumors. In line with this, most screened embryonal tumors displayed resistance to MR1T-targeting <jats:italic>in vitro<\/jats:italic>. MR1T susceptibility was observed particularly in pediatric leukemia and diffuse midline glioma models. This study demonstrates potential of MC.7.G5 MR1T-cell immunotherapy in pediatric leukemias and diffuse midline glioma, while activity against embryonal tumors was limited. The dismal prognosis associated with relapsed\/refractory leukemias and high-grade brain tumors highlights the promise to improve survival rates of children with these cancers.<\/jats:p>","DOI":"10.1136\/jitc-2023-007538","type":"journal-article","created":{"date-parts":[[2024,3,22]],"date-time":"2024-03-22T02:05:22Z","timestamp":1711073122000},"page":"e007538","update-policy":"https:\/\/doi.org\/10.1136\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":5,"title":["Targeting pediatric cancers via T-cell recognition of the monomorphic MHC class I-related protein MR1"],"prefix":"10.1136","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8349-121X","authenticated-orcid":false,"given":"Annelisa M.","family":"Cornel","sequence":"first","affiliation":[{"name":"Prinses Maxima Centrum voor Kinderoncologie, Utrecht, The Netherlands"},{"name":"Center for Translational Immunology, UMC Utrecht, Utrecht, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Loutje","family":"van der Sman","sequence":"additional","affiliation":[{"name":"Prinses Maxima Centrum voor Kinderoncologie, Utrecht, The Netherlands"},{"name":"Center for Translational Immunology, UMC Utrecht, Utrecht, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jip T","family":"van Dinter","sequence":"additional","affiliation":[{"name":"Center for Translational Immunology, UMC Utrecht, Utrecht, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marta","family":"Arrabito","sequence":"additional","affiliation":[{"name":"Center for Translational Immunology, UMC Utrecht, Utrecht, The Netherlands"},{"name":"Center of Pediatric Hematology & Oncology, University of Catania, Catania, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ester","family":"Dunnebach","sequence":"additional","affiliation":[{"name":"Prinses Maxima Centrum voor Kinderoncologie, Utrecht, The Netherlands"},{"name":"Center for Translational Immunology, UMC Utrecht, Utrecht, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marliek","family":"van Hoesel","sequence":"additional","affiliation":[{"name":"Prinses Maxima Centrum voor Kinderoncologie, Utrecht, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas A","family":"Kluiver","sequence":"additional","affiliation":[{"name":"Prinses Maxima Centrum voor Kinderoncologie, Utrecht, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana P","family":"Lopes","sequence":"additional","affiliation":[{"name":"Prinses Maxima Centrum voor Kinderoncologie, Utrecht, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"No\u00ebl M M","family":"Dautzenberg","sequence":"additional","affiliation":[{"name":"Center for Translational Immunology, UMC Utrecht, Utrecht, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linde","family":"Dekker","sequence":"additional","affiliation":[{"name":"Center for Translational Immunology, UMC Utrecht, Utrecht, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jorik M","family":"van Rijn","sequence":"additional","affiliation":[{"name":"Center for Translational Immunology, UMC Utrecht, Utrecht, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Denise A M H","family":"van den Beemt","sequence":"additional","affiliation":[{"name":"Prinses Maxima Centrum voor Kinderoncologie, Utrecht, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juliane L","family":"Buhl","sequence":"additional","affiliation":[{"name":"Center for Translational Immunology, UMC Utrecht, Utrecht, The Netherlands"},{"name":"Oncode Institute, Utrecht, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aimee","family":"du Chatinier","sequence":"additional","affiliation":[{"name":"Center for Translational Immunology, UMC Utrecht, Utrecht, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Farnaz","family":"Barneh","sequence":"additional","affiliation":[{"name":"Center for Translational Immunology, UMC Utrecht, Utrecht, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuyan","family":"Lu","sequence":"additional","affiliation":[{"name":"Center for Translational Immunology, UMC Utrecht, Utrecht, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luca","family":"Lo Nigro","sequence":"additional","affiliation":[{"name":"Center of Pediatric Hematology & Oncology, University of Catania, Catania, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anja","family":"Krippner-Heidenreich","sequence":"additional","affiliation":[{"name":"Center for Translational Immunology, UMC Utrecht, Utrecht, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zsolt","family":"Sebesty\u00e9n","sequence":"additional","affiliation":[{"name":"Prinses Maxima Centrum voor Kinderoncologie, Utrecht, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3914-7806","authenticated-orcid":false,"given":"Jurgen","family":"Kuball","sequence":"additional","affiliation":[{"name":"Prinses Maxima Centrum voor Kinderoncologie, Utrecht, The Netherlands"},{"name":"Department of Hematology, UMC Utrecht, Utrecht, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Esther","family":"Hulleman","sequence":"additional","affiliation":[{"name":"Center for Translational Immunology, UMC Utrecht, Utrecht, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jarno","family":"Drost","sequence":"additional","affiliation":[{"name":"Center for Translational Immunology, UMC Utrecht, Utrecht, The Netherlands"},{"name":"Oncode Institute, Utrecht, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sebastiaan","family":"van Heesch","sequence":"additional","affiliation":[{"name":"Center for Translational Immunology, UMC Utrecht, Utrecht, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olaf T","family":"Heidenreich","sequence":"additional","affiliation":[{"name":"Center for Translational Immunology, UMC Utrecht, Utrecht, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weng Chuan","family":"Peng","sequence":"additional","affiliation":[{"name":"Center for Translational Immunology, UMC Utrecht, Utrecht, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefan","family":"Nierkens","sequence":"additional","affiliation":[{"name":"Prinses Maxima Centrum voor Kinderoncologie, Utrecht, The Netherlands"},{"name":"Center for Translational Immunology, UMC Utrecht, Utrecht, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"239","published-online":{"date-parts":[[2024,3,21]]},"reference":[{"key":"2025100312483686000_12.3.e007538.1","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1111\/tan.14390","article-title":"MR1 encompasses at least six allele groups with coding region alterations","volume":"98","author":"Rozemuller","year":"2021","journal-title":"HLA"},{"key":"2025100312483686000_12.3.e007538.2","doi-asserted-by":"crossref","first-page":"4066","DOI":"10.4049\/jimmunol.161.8.4066","article-title":"Genomics, isoforms, expression, and phylogeny of the MHC class I-related MR1 gene","volume":"161","author":"Riegert","year":"1998","journal-title":"J Immunol"},{"key":"2025100312483686000_12.3.e007538.3","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.24476","article-title":"Functionally diverse human T cells recognize non-microbial antigens presented by MR1","volume":"6","author":"Lepore","year":"2017","journal-title":"Elife"},{"key":"2025100312483686000_12.3.e007538.4","doi-asserted-by":"publisher","DOI":"10.1111\/imm.12744"},{"key":"2025100312483686000_12.3.e007538.5","doi-asserted-by":"crossref","DOI":"10.3389\/fimmu.2020.00751","article-title":"MR1-restricted T cells are unprecedented cancer fighters","volume":"11","author":"Vacchini","year":"2020","journal-title":"Front Immunol"},{"key":"2025100312483686000_12.3.e007538.6","doi-asserted-by":"publisher","DOI":"10.1038\/s41590-019-0578-8"},{"key":"2025100312483686000_12.3.e007538.7","doi-asserted-by":"crossref","DOI":"10.3390\/cancers12082145","article-title":"MR1-restricted T cells in cancer immunotherapy","volume":"12","author":"Flores-Villanueva","year":"2020","journal-title":"Cancers (Basel)"},{"key":"2025100312483686000_12.3.e007538.8","doi-asserted-by":"crossref","unstructured":"Vacchini A , Yang Q , Chancellor A , et al . Nucleobase adduct-containing metabolites are MR1 ligands that stimulate self-reactive MR1t cells. BioRxiv 2022. doi:10.1101\/2022.08.26.505411","DOI":"10.1101\/2022.08.26.505411"},{"key":"2025100312483686000_12.3.e007538.9","doi-asserted-by":"publisher","DOI":"10.1016\/j.coi.2020.12.002"},{"key":"2025100312483686000_12.3.e007538.10","doi-asserted-by":"crossref","first-page":"673","DOI":"10.1007\/s10555-019-09836-y","article-title":"Compare and contrast: pediatric cancer versus adult malignancies","volume":"38","author":"Kattner","year":"2019","journal-title":"Cancer Metastasis Rev"},{"key":"2025100312483686000_12.3.e007538.11","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3390\/biomedinformatics3010001","article-title":"Trecode: a FAIR ECO-system for the analysis and archiving of omics data in a combined diagnostic and research setting","volume":"3","author":"Kerstens","year":"2023","journal-title":"BioMedInformatics"},{"key":"2025100312483686000_12.3.e007538.12","doi-asserted-by":"crossref","DOI":"10.1186\/s12885-023-11054-3","article-title":"Systematic discovery of gene fusions in pediatric cancer by integrating RNA-Seq and WGS","volume":"23","author":"van Belzen","year":"2023","journal-title":"BMC Cancer"},{"key":"2025100312483686000_12.3.e007538.13","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bts635"},{"key":"2025100312483686000_12.3.e007538.14","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btt656"},{"key":"2025100312483686000_12.3.e007538.15","doi-asserted-by":"crossref","unstructured":"Kellaway SG , Potluri S , Keane P , et al . Leukemic stem cells hijack lineage inappropriate signalling pathways to promote their growth. BioRxiv 2023. doi:10.1101\/2023.03.10.532081","DOI":"10.1101\/2023.03.10.532081"},{"key":"2025100312483686000_12.3.e007538.16","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3390\/cancers12092668","article-title":"A human organoid model of aggressive hepatoblastoma for disease modeling and drug testing","volume":"12","author":"Saltsman","year":"2020","journal-title":"Cancers (Basel)"},{"key":"2025100312483686000_12.3.e007538.17","doi-asserted-by":"crossref","unstructured":"Kluiver TA , Lu Y , Schubert SA , et al . Multi-dimensional profiling of hepatoblastomas and patient-derived tumor organoids uncovers tumor subpopulations with divergent WNT activation profiles and identifies pan-hepatoblastoma drug sensitivities. BioRxiv 2023. doi:10.1101\/2023.08.28.554783","DOI":"10.1101\/2023.08.28.554783"},{"key":"2025100312483686000_12.3.e007538.18","doi-asserted-by":"crossref","first-page":"840","DOI":"10.1016\/j.stem.2020.07.020","article-title":"Next-generation surrogate Wnts support organoid growth and deconvolute frizzled pleiotropy in vivo","volume":"27","author":"Miao","year":"2020","journal-title":"Cell Stem Cell"},{"key":"2025100312483686000_12.3.e007538.19","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/978-1-0716-2557-6_1","article-title":"Long-term expansion of murine primary hepatocyte organoids","volume":"2544","author":"Kluiver","year":"2022","journal-title":"Methods Mol Biol"},{"key":"2025100312483686000_12.3.e007538.20","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejca.2013.11.015"},{"key":"2025100312483686000_12.3.e007538.21","doi-asserted-by":"crossref","first-page":"109568","DOI":"10.1016\/j.celrep.2021.109568","article-title":"Organoid-based drug screening reveals neddylation as therapeutic target for malignant rhabdoid tumors","volume":"36","author":"Calandrini","year":"2021","journal-title":"Cell Reports"},{"key":"2025100312483686000_12.3.e007538.22","doi-asserted-by":"crossref","DOI":"10.1038\/s41467-020-15155-6","article-title":"An organoid biobank for childhood kidney cancers that captures disease and tissue heterogeneity","volume":"11","author":"Calandrini","year":"2020","journal-title":"Nat Commun"},{"key":"2025100312483686000_12.3.e007538.23","doi-asserted-by":"crossref","first-page":"778","DOI":"10.1097\/NEN.0000000000000216","article-title":"Disrupting NOTCH Slows diffuse intrinsic pontine glioma growth, enhances radiation sensitivity, and shows combinatorial efficacy with bromodomain inhibition","volume":"74","author":"Taylor","year":"2015","journal-title":"J Neuropathol Exp Neurol"},{"key":"2025100312483686000_12.3.e007538.24","doi-asserted-by":"crossref","first-page":"1204","DOI":"10.1038\/s41591-018-0086-7","article-title":"Functional diversity and cooperativity between subclonal populations of pediatric glioblastoma and diffuse intrinsic pontine glioma cells","volume":"24","author":"Vinci","year":"2018","journal-title":"Nat Med"},{"key":"2025100312483686000_12.3.e007538.25","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1016\/j.omtm.2021.03.015","article-title":"Efficient lentiviral transduction method to gene modify cord blood CD8+ T cells for cancer therapy applications","volume":"21","author":"Lo Presti","year":"2021","journal-title":"Mol Ther Methods Clin Dev"},{"key":"2025100312483686000_12.3.e007538.26","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.72.12.9873-9880.1998"},{"key":"2025100312483686000_12.3.e007538.27","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.72.11.8463-8471.1998"},{"key":"2025100312483686000_12.3.e007538.28","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2010-12-325993"},{"key":"2025100312483686000_12.3.e007538.29","doi-asserted-by":"crossref","DOI":"10.1136\/jitc-2022-005002","article-title":"Epigenetic modulation of neuroblastoma enhances T cell and NK cell immunogenicity by inducing a tumor-cell lineage switch","volume":"10","author":"Cornel","year":"2022","journal-title":"J Immunother Cancer"},{"key":"2025100312483686000_12.3.e007538.30","doi-asserted-by":"crossref","DOI":"10.3389\/fimmu.2020.559152","article-title":"Clinical grade production of Wilms\u2019 Tumor-1 loaded cord blood-derived dendritic cells to prevent relapse in pediatric AML after cord blood transplantation","volume":"11","author":"Plantinga","year":"2020","journal-title":"Front Immunol"},{"key":"2025100312483686000_12.3.e007538.31","doi-asserted-by":"crossref","first-page":"1228","DOI":"10.1038\/s43018-022-00427-5","article-title":"Mesenchymal and adrenergic cell lineage States in neuroblastoma possess distinct immunogenic phenotypes","volume":"3","author":"Sengupta","year":"2022","journal-title":"Nat Cancer"},{"key":"2025100312483686000_12.3.e007538.32","doi-asserted-by":"publisher","DOI":"10.1016\/J.CELL.2021.04.048"},{"key":"2025100312483686000_12.3.e007538.33","doi-asserted-by":"crossref","unstructured":"Korotkevich G , Sukhov V , Budin N , et al . Fast gene set enrichment analysis. BioRxiv 2021. doi:10.1101\/060012","DOI":"10.1101\/060012"},{"key":"2025100312483686000_12.3.e007538.34","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3389\/fcell.2021.640633","article-title":"In vitro modeling of embryonal tumors","volume":"9","author":"Custers","year":"2021","journal-title":"Front Cell Dev Biol"},{"key":"2025100312483686000_12.3.e007538.35","doi-asserted-by":"crossref","first-page":"575","DOI":"10.1016\/j.ccell.2019.11.006","article-title":"When cold is hot: immune checkpoint inhibition therapy for rhabdoid tumors","volume":"36","author":"Yarmarkovich","year":"2019","journal-title":"Cancer Cell"},{"key":"2025100312483686000_12.3.e007538.36","doi-asserted-by":"crossref","first-page":"842","DOI":"10.1038\/s41593-020-0628-4","article-title":"Tumor necrosis factor overcomes immune evasion in P53-mutant medulloblastoma","volume":"23","author":"Garancher","year":"2020","journal-title":"Nat Neurosci"},{"key":"2025100312483686000_12.3.e007538.37","doi-asserted-by":"crossref","first-page":"iii363","DOI":"10.1093\/neuonc\/noaa222.375","article-title":"IMMU-19. HDAC inhibitors sensitize MYC-amplified medulloblastoma to immunotherapy by activating the NF-KB pathways","volume":"22","author":"Marquardt","year":"2020","journal-title":"Neuro-Oncology"},{"key":"2025100312483686000_12.3.e007538.38","doi-asserted-by":"crossref","first-page":"1407","DOI":"10.1038\/s41467-021-21675-6","article-title":"Somatic mutations and single-cell transcriptomes reveal the root of malignant rhabdoid tumours","volume":"12","author":"Custers","year":"2021","journal-title":"Nat Commun"},{"key":"2025100312483686000_12.3.e007538.39","doi-asserted-by":"publisher","DOI":"10.1038\/nature25480"},{"key":"2025100312483686000_12.3.e007538.40","doi-asserted-by":"publisher","DOI":"10.1038\/nature25795"},{"key":"2025100312483686000_12.3.e007538.41","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-14-1860"},{"key":"2025100312483686000_12.3.e007538.42","doi-asserted-by":"publisher","DOI":"10.1038\/ng.3899"},{"key":"2025100312483686000_12.3.e007538.43","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1177\/1093526619875228","article-title":"Hepatoblastoma and pediatric hepatocellular carcinoma: an update","volume":"23","author":"Ranganathan","year":"2020","journal-title":"Pediatr Dev Pathol"},{"key":"2025100312483686000_12.3.e007538.44","doi-asserted-by":"crossref","first-page":"2031","DOI":"10.1182\/blood.V77.9.2031.2031","article-title":"Establishment of a human acute myeloid leukemia cell line (Kasumi-1) with 8;21 chromosome translocation","volume":"77","author":"Asou","year":"1991","journal-title":"Blood"},{"key":"2025100312483686000_12.3.e007538.45","doi-asserted-by":"crossref","DOI":"10.1126\/sciimmunol.abc9492","article-title":"Absence of mucosal-associated invariant T cells in a person with a homozygous point mutation in MR1","volume":"5","author":"Howson","year":"2020","journal-title":"Sci Immunol"},{"key":"2025100312483686000_12.3.e007538.46","doi-asserted-by":"crossref","unstructured":"Cornforth TV , Moyo N , Cole S , et al . Conserved allomorphs of MR1 drive specificity of MR1-restricted TCRs. BioRxiv 2023. doi:10.1101\/2023.07.17.548997","DOI":"10.1101\/2023.07.17.548997"}],"container-title":["Journal for ImmunoTherapy of Cancer"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1136\/jitc-2023-007538","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,3]],"date-time":"2025-10-03T19:48:50Z","timestamp":1759520930000},"score":1,"resource":{"primary":{"URL":"https:\/\/jitc.bmj.com\/lookup\/doi\/10.1136\/jitc-2023-007538"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,3]]},"references-count":46,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2024,3,21]]},"published-print":{"date-parts":[[2024,3]]}},"alternative-id":["10.1136\/jitc-2023-007538"],"URL":"https:\/\/doi.org\/10.1136\/jitc-2023-007538","relation":{},"ISSN":["2051-1426"],"issn-type":[{"value":"2051-1426","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,3]]}}}